CA2425483A1 - Assay method utilizing induced luminescence - Google Patents

Assay method utilizing induced luminescence Download PDF

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Publication number
CA2425483A1
CA2425483A1 CA002425483A CA2425483A CA2425483A1 CA 2425483 A1 CA2425483 A1 CA 2425483A1 CA 002425483 A CA002425483 A CA 002425483A CA 2425483 A CA2425483 A CA 2425483A CA 2425483 A1 CA2425483 A1 CA 2425483A1
Authority
CA
Canada
Prior art keywords
analyte
chemiluminescent compound
amount
medium
photosensitizer
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Granted
Application number
CA002425483A
Other languages
French (fr)
Other versions
CA2425483C (en
Inventor
Edwin F. Ullman
Hrair Kirakossian
John S. Pease
Yuri Daniloff
Daniel B. Wagner
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Siemens Healthcare Diagnostics GmbH Germany
Original Assignee
Individual
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from US07/704,569 external-priority patent/US6251581B1/en
Priority claimed from US07/718,490 external-priority patent/US5340716A/en
Application filed by Individual filed Critical Individual
Priority claimed from CA002069145A external-priority patent/CA2069145C/en
Publication of CA2425483A1 publication Critical patent/CA2425483A1/en
Application granted granted Critical
Publication of CA2425483C publication Critical patent/CA2425483C/en
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

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Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/569—Immunoassay; Biospecific binding assay; Materials therefor for microorganisms, e.g. protozoa, bacteria, viruses
    • G01N33/56966—Animal cells
    • G01N33/56977—HLA or MHC typing

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Immunology (AREA)
  • Engineering & Computer Science (AREA)
  • Hematology (AREA)
  • Cell Biology (AREA)
  • Chemical & Material Sciences (AREA)
  • Urology & Nephrology (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • Virology (AREA)
  • Physics & Mathematics (AREA)
  • Biotechnology (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Zoology (AREA)
  • Food Science & Technology (AREA)
  • Medicinal Chemistry (AREA)
  • Microbiology (AREA)
  • Analytical Chemistry (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Investigating Or Analysing Materials By The Use Of Chemical Reactions (AREA)

Abstract

Methods are disclosed for determining an analyte in a medium suspected of containing the analyte. One method comprises treating a medium suspected of containing an analyte under conditions such that the analyte, if present, causes a photosensitizer and a chemiluminescent compound to come into close proximity. The photosensitizer generates singlet oxygen and activates the chemiluminescent compound when it is in close proximity. The activated chemiluminescent compound subsequently produces light. The amount of light produced is related to the amount of analyte in the medium. Preferably, at least one of the photosensitizer and chemiluminescent compound is associated with a surface which is usually a suspendible particle, and a specific binding pair member is bound thereto. Another method comprises providing (1) a medium suspected of containing the analyte, (2) a label reagent comprising a first specific binding pair (sbp) member associated with a photochemically activatable chemiluminescent compound wherein the first sbp member is capable of binding to the analyte or to a second sbp member to form a complex related to the presence of the analyte. This method further comprises photochemically activating the chemiluminescent compound. The amount of luminescence generated by the chemiluminescent compound is detected. The amount thereof is related to the amount of analyte in the medium. Compositions and kits are also disclosed.
CA002425483A 1991-05-22 1992-05-21 Assay method utilizing induced luminescence Expired - Lifetime CA2425483C (en)

Applications Claiming Priority (5)

Application Number Priority Date Filing Date Title
US704,569 1991-05-22
US07/704,569 US6251581B1 (en) 1991-05-22 1991-05-22 Assay method utilizing induced luminescence
US07/718,490 US5340716A (en) 1991-06-20 1991-06-20 Assay method utilizing photoactivated chemiluminescent label
US718,490 1991-06-20
CA002069145A CA2069145C (en) 1991-05-22 1992-05-21 Assay method utilizing induced luminescence

Related Parent Applications (1)

Application Number Title Priority Date Filing Date
CA002069145A Division CA2069145C (en) 1991-05-22 1992-05-21 Assay method utilizing induced luminescence

Publications (2)

Publication Number Publication Date
CA2425483A1 true CA2425483A1 (en) 1992-11-23
CA2425483C CA2425483C (en) 2008-07-29

Family

ID=27169086

Family Applications (1)

Application Number Title Priority Date Filing Date
CA002425483A Expired - Lifetime CA2425483C (en) 1991-05-22 1992-05-21 Assay method utilizing induced luminescence

Country Status (1)

Country Link
CA (1) CA2425483C (en)

Cited By (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN110736740A (en) * 2018-07-18 2020-01-31 博阳生物科技（上海）有限公司 A homogeneous chemiluminescence POCT detection method and device using the detection method
CN111665235A (en) * 2019-03-08 2020-09-15 上海索昕生物科技有限公司 Chemiluminescent microarray chip and application thereof
CN113125705A (en) * 2019-12-31 2021-07-16 博阳生物科技（上海）有限公司 Homogeneous detection kit for myoglobin and application thereof
CN113125703A (en) * 2019-12-31 2021-07-16 博阳生物科技（上海）有限公司 Homogeneous detection kit for myoglobin and application thereof
CN113495064A (en) * 2020-03-19 2021-10-12 财团法人工业技术研究院 Detection reagent, detection device and method for detecting primary amide compound

Cited By (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN110736740A (en) * 2018-07-18 2020-01-31 博阳生物科技（上海）有限公司 A homogeneous chemiluminescence POCT detection method and device using the detection method
CN111665235A (en) * 2019-03-08 2020-09-15 上海索昕生物科技有限公司 Chemiluminescent microarray chip and application thereof
CN113125705A (en) * 2019-12-31 2021-07-16 博阳生物科技（上海）有限公司 Homogeneous detection kit for myoglobin and application thereof
CN113125703A (en) * 2019-12-31 2021-07-16 博阳生物科技（上海）有限公司 Homogeneous detection kit for myoglobin and application thereof
CN113125703B (en) * 2019-12-31 2023-08-04 科美诊断技术(苏州)有限公司 Myoglobin homogeneous detection kit and application thereof
CN113125705B (en) * 2019-12-31 2023-08-04 科美诊断技术(苏州)有限公司 Myoglobin homogeneous detection kit and application thereof
CN113495064A (en) * 2020-03-19 2021-10-12 财团法人工业技术研究院 Detection reagent, detection device and method for detecting primary amide compound
CN113495064B (en) * 2020-03-19 2024-05-03 财团法人工业技术研究院 Detection reagent, detection device, and method for detecting primary amide compounds

Also Published As

Publication number Publication date
CA2425483C (en) 2008-07-29

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