CA2426959C - Animaux transgeniques utilises pour analyser la regulation du gene p450 du cytochrome cyp3a4 - Google Patents

Animaux transgeniques utilises pour analyser la regulation du gene p450 du cytochrome cyp3a4 Download PDF

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Publication number
CA2426959C
CA2426959C CA2426959A CA2426959A CA2426959C CA 2426959 C CA2426959 C CA 2426959C CA 2426959 A CA2426959 A CA 2426959A CA 2426959 A CA2426959 A CA 2426959A CA 2426959 C CA2426959 C CA 2426959C
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CA
Canada
Prior art keywords
nucleic acid
acid molecule
reporter
human
transcription
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Fee Related
Application number
CA2426959A
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English (en)
Other versions
CA2426959A1 (fr
Inventor
Christopher Liddle
Bryan James Goodwin
Graham Robertson
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
The University of Sydney
Original Assignee
The University of Sydney
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from AUPR1161A external-priority patent/AUPR116100A0/en
Priority claimed from AUPR4901A external-priority patent/AUPR490101A0/en
Application filed by The University of Sydney filed Critical The University of Sydney
Publication of CA2426959A1 publication Critical patent/CA2426959A1/fr
Application granted granted Critical
Publication of CA2426959C publication Critical patent/CA2426959C/fr
Anticipated expiration legal-status Critical
Expired - Fee Related legal-status Critical Current

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Classifications

    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K49/00—Preparations for testing in vivo
    • A61K49/0004—Screening or testing of compounds for diagnosis of disorders, assessment of conditions, e.g. renal clearance, gastric emptying, testing for diabetes, allergy, rheuma, pancreas functions
    • A61K49/0008—Screening agents using (non-human) animal models or transgenic animal models or chimeric hosts, e.g. Alzheimer disease animal model, transgenic model for heart failure
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K67/00—Rearing or breeding animals, not otherwise provided for; New or modified breeds of animals
    • A01K67/027—New or modified breeds of vertebrates
    • A01K67/0275—Genetically modified vertebrates, e.g. transgenic
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/85—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
    • C12N15/8509—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells for producing genetically modified animals, e.g. transgenic
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C12N9/0071—Oxidoreductases (1.) acting on paired donors with incorporation of molecular oxygen (1.14)
    • C12N9/0077—Oxidoreductases (1.) acting on paired donors with incorporation of molecular oxygen (1.14) with a reduced iron-sulfur protein as one donor (1.14.15)
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K2217/00—Genetically modified animals
    • A01K2217/05—Animals comprising random inserted nucleic acids (transgenic)
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K2227/00—Animals characterised by species
    • A01K2227/10—Mammal
    • A01K2227/105—Murine
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K2267/00—Animals characterised by purpose
    • A01K2267/03—Animal model, e.g. for test or diseases
    • A01K2267/0393—Animal model comprising a reporter system for screening tests

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Genetics & Genomics (AREA)
  • Chemical & Material Sciences (AREA)
  • Biomedical Technology (AREA)
  • Organic Chemistry (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Health & Medical Sciences (AREA)
  • Biotechnology (AREA)
  • Veterinary Medicine (AREA)
  • General Engineering & Computer Science (AREA)
  • Biochemistry (AREA)
  • Environmental Sciences (AREA)
  • Microbiology (AREA)
  • Animal Behavior & Ethology (AREA)
  • Molecular Biology (AREA)
  • Rheumatology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Public Health (AREA)
  • Plant Pathology (AREA)
  • Epidemiology (AREA)
  • Urology & Nephrology (AREA)
  • Toxicology (AREA)
  • Pathology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Endocrinology (AREA)
  • Animal Husbandry (AREA)
  • Biodiversity & Conservation Biology (AREA)
  • Diabetes (AREA)
  • Medicinal Chemistry (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Investigating Or Analysing Biological Materials (AREA)

Abstract

La présente invention concerne la génération d'animaux transgéniques non humains comprenant une construction de rapporteur en vue de produire une quantité détectable d'une molécule rapporteur fonctionnellement liée à une molécule d'acide nucléique de régulation transcriptionnelle provenant du gène humain CYP3A4 situé entre le début du site de transcription du gène et un endroit situé 13 000 nucléotides en amont du site. Cette invention concerne également l'utilisation de ces animaux pour déterminer l'effet d'un composé, notamment mais pas uniquement, d'un xénobiotique ou d'un stéroïde, sur la régulation de l'expression du gène CYP3A4 chez l'homme.
CA2426959A 2000-11-01 2001-11-01 Animaux transgeniques utilises pour analyser la regulation du gene p450 du cytochrome cyp3a4 Expired - Fee Related CA2426959C (fr)

Applications Claiming Priority (5)

Application Number Priority Date Filing Date Title
AUPR1161A AUPR116100A0 (en) 2000-11-01 2000-11-01 P450 gene regulation
AUPR1161 2000-11-01
AUPR4901 2001-05-10
AUPR4901A AUPR490101A0 (en) 2001-05-10 2001-05-10 P450 gene regulation ii
PCT/AU2001/001407 WO2002036784A1 (fr) 2000-11-01 2001-11-01 Animaux transgeniques utilises pour analyser la regulation du gene p450 du cytochrome cyp3a4

Publications (2)

Publication Number Publication Date
CA2426959A1 CA2426959A1 (fr) 2002-05-10
CA2426959C true CA2426959C (fr) 2011-10-04

Family

ID=27664007

Family Applications (1)

Application Number Title Priority Date Filing Date
CA2426959A Expired - Fee Related CA2426959C (fr) 2000-11-01 2001-11-01 Animaux transgeniques utilises pour analyser la regulation du gene p450 du cytochrome cyp3a4

Country Status (5)

Country Link
EP (1) EP1337650A4 (fr)
JP (2) JP2004512054A (fr)
AU (2) AU2002213660A1 (fr)
CA (1) CA2426959C (fr)
WO (1) WO2002036784A1 (fr)

Families Citing this family (10)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
AUPP362898A0 (en) 1998-05-21 1998-06-11 University Of Sydney, The Xenobiotic induction of gene expression
AUPR116100A0 (en) 2000-11-01 2000-11-23 University Of Sydney, The P450 gene regulation
EP1390744B1 (fr) * 2001-04-12 2012-09-26 Xenogen Corporation Isolation et identification d'elements regulateurs de transcription murins et humains associes a l'expression de cytochromes
GB0216203D0 (en) * 2002-07-12 2002-08-21 Imp Cancer Res Tech Mondulation of cytochrome P450 reductase activity
US7700822B2 (en) 2002-07-12 2010-04-20 Cancer Research Technology Limited Modulation of cytochrome P450 reductase activity
WO2006064197A2 (fr) * 2004-12-13 2006-06-22 Iti Scotland Limited Animaux transgeniques pour evaluer le metabolisme et la toxicite d'un medicament
US7759541B2 (en) 2004-12-13 2010-07-20 Iti Life Sciences Transgenic animals for assessing drug metabolism and toxicity
KR100811184B1 (ko) * 2005-10-21 2008-03-07 삼성전자주식회사 아우터 인코더 및 그 방법
US20150284812A1 (en) 2012-10-19 2015-10-08 National University Corporation Tottori University Method for evaluating both induction of drug-metabolizing enzyme and cytotoxicity, and vector and cell for use in said method
JP6868237B2 (ja) * 2016-10-11 2021-05-12 国立大学法人鳥取大学 ヒトの薬物代謝酵素誘導及び薬物動態予測が可能な新規薬物代謝酵素誘導評価方法

Family Cites Families (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
AU3211699A (en) * 1998-03-27 1999-10-18 Glaxo Group Limited Orphan nuclear receptor
AUPP362898A0 (en) * 1998-05-21 1998-06-11 University Of Sydney, The Xenobiotic induction of gene expression
EP1272846A4 (fr) * 2000-04-12 2004-04-28 Puracyp Compositions et procedes d'induction de proteines impliquees dans le metabolisme xenobiotique
EP1390744B1 (fr) * 2001-04-12 2012-09-26 Xenogen Corporation Isolation et identification d'elements regulateurs de transcription murins et humains associes a l'expression de cytochromes

Also Published As

Publication number Publication date
AU2002213660A1 (en) 2002-05-15
EP1337650A4 (fr) 2005-12-21
AU2007249083A1 (en) 2008-01-10
JP5543771B2 (ja) 2014-07-09
JP2010148508A (ja) 2010-07-08
CA2426959A1 (fr) 2002-05-10
WO2002036784A1 (fr) 2002-05-10
EP1337650A1 (fr) 2003-08-27
AU2007249083B8 (en) 2011-02-03
AU2007249083B2 (en) 2010-12-16
JP2004512054A (ja) 2004-04-22

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