CA2428441A1 - Procedes, dispositifs, reseaux et kits permettant de detecter et d'analyser des biomolecules - Google Patents

Procedes, dispositifs, reseaux et kits permettant de detecter et d'analyser des biomolecules Download PDF

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Publication number
CA2428441A1
CA2428441A1 CA002428441A CA2428441A CA2428441A1 CA 2428441 A1 CA2428441 A1 CA 2428441A1 CA 002428441 A CA002428441 A CA 002428441A CA 2428441 A CA2428441 A CA 2428441A CA 2428441 A1 CA2428441 A1 CA 2428441A1
Authority
CA
Canada
Prior art keywords
membranes
biomolecules
membrane
protein
sample
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Abandoned
Application number
CA002428441A
Other languages
English (en)
Inventor
Vladimir Knezevic
Michael R. Emmert-Buck
Galina Baibakova
Dan-Paul Hartmann
Stephen Hewitt
Capre Mitchell
Kevin Gardner
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
20 20 GeneSystems Inc
National Institutes of Health NIH
Original Assignee
Individual
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from US09/718,990 external-priority patent/US6602661B1/en
Priority claimed from US09/753,574 external-priority patent/US20020012920A1/en
Application filed by Individual filed Critical Individual
Publication of CA2428441A1 publication Critical patent/CA2428441A1/fr
Abandoned legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C40—COMBINATORIAL TECHNOLOGY
    • C40B—COMBINATORIAL CHEMISTRY; LIBRARIES, e.g. CHEMICAL LIBRARIES
    • C40B30/00—Methods of screening libraries
    • C40B30/04—Methods of screening libraries by measuring the ability to specifically bind a target molecule, e.g. antibody-antigen binding, receptor-ligand binding
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N27/00—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
    • G01N27/26—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating electrochemical variables; by using electrolysis or electrophoresis
    • G01N27/416—Systems
    • G01N27/447—Systems using electrophoresis
    • G01N27/44704—Details; Accessories
    • G01N27/44717—Arrangements for investigating the separated zones, e.g. localising zones
    • G01N27/44739—Collecting the separated zones, e.g. blotting to a membrane or punching of gel spots
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/68—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
    • G01N33/6803—General methods of protein analysis not limited to specific proteins or families of proteins
    • G01N33/6845—Methods of identifying protein-protein interactions in protein mixtures

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Molecular Biology (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Immunology (AREA)
  • General Health & Medical Sciences (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • Medicinal Chemistry (AREA)
  • General Physics & Mathematics (AREA)
  • Analytical Chemistry (AREA)
  • Pathology (AREA)
  • Urology & Nephrology (AREA)
  • Hematology (AREA)
  • Organic Chemistry (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Biomedical Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Biophysics (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • Cell Biology (AREA)
  • Electrochemistry (AREA)
  • Microbiology (AREA)
  • General Chemical & Material Sciences (AREA)
  • Food Science & Technology (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

L'invention concerne des dispositifs, des réseaux, des kits et des procédés permettant de détecter des biomolécules dans une section tissulaire (telle qu'un échantillon frais ou d'archives, un micro-réseau tissulaire ou des cellules rassemblées au moyen d'une procédure LCM) ou dans d'autres échantillons sensiblement bidimensionnels (tels qu'un gel électrophorétique ou un micro-réseau d'ADNc) par création de "copies à carbone" des biomolécules eluées à partir de l'échantillon et par visualisation des biomolécules sur les copies, au moyen d'au moins une molécule de détention (par exemple, des anticorps ou des sondes d'ADN) présentant une affinité spécifique pour les biomolécules à étudier. L'invention concerne également des procédés spécifiques permettant d'identifier le schéma des biomolécules (par exemple, des protéines et des acides nucléiques) dans les échantillons. L'invention concerne, en outre, d'autres procédés spécifiques permettant d'identifier et d'analyser des protéines et d'autres molécules biologiques produites par des cellules et/ou un tissu, notamment des cellules et/ou un tissu humains. L'invention concerne enfin une pluralité de membranes préparées et/ou traitées de manière différente, ces membranes pouvant être utilisées dans les procédés selon l'invention et permettant d'identifier et d'analyser des biomolécules.
CA002428441A 2000-11-20 2001-11-20 Procedes, dispositifs, reseaux et kits permettant de detecter et d'analyser des biomolecules Abandoned CA2428441A1 (fr)

Applications Claiming Priority (11)

Application Number Priority Date Filing Date Title
US09/718,990 US6602661B1 (en) 1999-07-26 2000-11-20 Methods and arrays for detecting biomolecules
US09/718,990 2000-11-20
US09/753,574 US20020012920A1 (en) 2000-11-20 2001-01-04 Method and kit for proteomic identification
US09/753,574 2001-01-04
US28625801P 2001-04-25 2001-04-25
US60/286,258 2001-04-25
US29647501P 2001-06-08 2001-06-08
US60/296,475 2001-06-08
US30403101P 2001-07-09 2001-07-09
US60/304,031 2001-07-09
PCT/US2001/044009 WO2002048674A2 (fr) 2000-11-20 2001-11-20 Procedes, dispositifs, reseaux et kits permettant de detecter et d'analyser des biomolecules

Publications (1)

Publication Number Publication Date
CA2428441A1 true CA2428441A1 (fr) 2002-06-20

Family

ID=27540697

Family Applications (1)

Application Number Title Priority Date Filing Date
CA002428441A Abandoned CA2428441A1 (fr) 2000-11-20 2001-11-20 Procedes, dispositifs, reseaux et kits permettant de detecter et d'analyser des biomolecules

Country Status (5)

Country Link
EP (1) EP1358314A4 (fr)
JP (1) JP2004518949A (fr)
AU (2) AU4323602A (fr)
CA (1) CA2428441A1 (fr)
WO (1) WO2002048674A2 (fr)

Families Citing this family (22)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US7838222B2 (en) * 1999-07-26 2010-11-23 United States of America/ NIH Methods, devices and kits for multiplex blotting of biological samples from multi-well plates
GB2391867A (en) * 2002-08-13 2004-02-18 Smartbead Technologies Ltd Analysis system using coded supports
JP4007269B2 (ja) * 2003-07-29 2007-11-14 オムロン株式会社 生体分子の形状転写方法、チップ基板の製造方法及びバイオチップの製造方法
US8012693B2 (en) 2003-12-16 2011-09-06 3M Innovative Properties Company Analysis of chemically crosslinked cellular samples
AU2005230642B2 (en) * 2004-04-05 2008-06-05 Proteome Systems Ltd. Improved diagnostic testing apparatus
EP1756568A1 (fr) * 2004-04-05 2007-02-28 Proteome Systems Intellectual Property Pty Ltd. Appareil de test diagnostique ameliore
GB2435510A (en) 2006-02-23 2007-08-29 Mologic Ltd Enzyme detection product and methods
GB2435511A (en) 2006-02-23 2007-08-29 Mologic Ltd Protease detection
GB2435512A (en) 2006-02-23 2007-08-29 Mologic Ltd A binding assay and assay device
US8075755B2 (en) * 2007-12-13 2011-12-13 Bio-Rad Laboratories, Inc. Polymeric sorbent sheets as ion reservoirs for electroblotting
JP2010096529A (ja) * 2008-10-14 2010-04-30 Toppan Printing Co Ltd 転写用積層体、電気泳動兼転写用積層体および電気泳動兼転写用チップ
US8524507B2 (en) 2009-08-19 2013-09-03 The Cleveland Clinic Foundation Method for detecting a target molecule in a biological sample
EP2400304A1 (fr) * 2010-06-22 2011-12-28 Centro de Investigación Cooperativa En Biomateriales ( CIC biomaGUNE) Procédé de caractérisation d'interactions intermoléculaires
JP2013178140A (ja) * 2012-02-28 2013-09-09 Sharp Corp 核酸−タンパク質複合体の単離装置
IN2013DE03479A (fr) 2013-11-29 2015-06-05 Ge Healthcare Bio Sciences Ab
WO2015078781A1 (fr) * 2013-11-29 2015-06-04 Ge Healthcare Bio-Sciences Ab Système intégré pour électrobuvardage
GB201411624D0 (en) * 2014-06-30 2014-08-13 Ge Healthcare Uk Ltd Spatial molecular profiling of solid biological masses and profile storage
WO2019024990A1 (fr) * 2017-08-01 2019-02-07 Albert-Ludwigs-Universität Freiburg Séparation électrophorétique de biomolécules
WO2019217396A1 (fr) * 2018-05-08 2019-11-14 Yale University Membrane de capture de protéine et son procédé d'utilisation
CN112354572B (zh) * 2019-07-11 2022-02-11 北京理工大学 一种多维微流控电泳芯片及检测装置，检测方法
CN114636818B (zh) * 2022-05-19 2022-07-29 天津德祥生物技术有限公司 一种包被液及含有该包被液的红细胞膜包被液及其应用
CN117168955B (zh) * 2023-09-13 2026-04-21 四川大学华西医院 一种改良的空腔脏器组织冰冻包埋方法

Family Cites Families (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5438128A (en) * 1992-02-07 1995-08-01 Millipore Corporation Method for rapid purifiction of nucleic acids using layered ion-exchange membranes
US5843657A (en) * 1994-03-01 1998-12-01 The United States Of America As Represented By The Department Of Health And Human Services Isolation of cellular material under microscopic visualization
GB9624927D0 (en) * 1996-11-29 1997-01-15 Oxford Glycosciences Uk Ltd Gels and their use
US5993627A (en) * 1997-06-24 1999-11-30 Large Scale Biology Corporation Automated system for two-dimensional electrophoresis
US6013165A (en) * 1998-05-22 2000-01-11 Lynx Therapeutics, Inc. Electrophoresis apparatus and method
US6135942A (en) * 1998-11-30 2000-10-24 Leptin; Maria Nucleic acids proteins of a D. melanogaster insulin-like gene and uses thereof
JP2002535676A (ja) * 1999-01-28 2002-10-22 ユニヴェルシテ ドゥ ジュネーブ ポリペプチドを同定または特徴付けるための方法およびキット

Also Published As

Publication number Publication date
AU4323602A (en) 2002-06-24
EP1358314A4 (fr) 2008-06-25
AU2002243236B2 (en) 2007-11-08
WO2002048674A2 (fr) 2002-06-20
EP1358314A2 (fr) 2003-11-05
JP2004518949A (ja) 2004-06-24
WO2002048674A3 (fr) 2002-09-06

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Legal Events

Date Code Title Description
EEER Examination request
FZDE Discontinued