CA2437473A1 - Methode pour conserver des cellules et des cibles d'acide nucleique - Google Patents

Methode pour conserver des cellules et des cibles d'acide nucleique Download PDF

Info

Publication number: CA2437473A1
Authority: CA; Canada
Prior art keywords: sample; nucleic acid; composition; proteolytic; agents
Prior art date: 2002-08-20
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.): Abandoned

Application number

CA 2437473

Other languages

English (en)

Inventor

Ray A. Mcmillian

Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)

Becton Dickinson and Co

Original Assignee

Individual

Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)

2002-08-20

Filing date

2003-08-18

Publication date

2004-02-20

2002-08-20 Priority claimed from US10/225,065 external-priority patent/US7029840B2/en

2003-08-18 Application filed by Individual filed Critical Individual

2004-02-20 Publication of CA2437473A1 publication Critical patent/CA2437473A1/fr

Status Abandoned legal-status Critical Current

Links

238000000034 method Methods 0.000 title claims abstract description 76
150000007523 nucleic acids Chemical class 0.000 title claims abstract description 64
108020004707 nucleic acids Proteins 0.000 title claims abstract description 61
102000039446 nucleic acids Human genes 0.000 title claims abstract description 61
238000004321 preservation Methods 0.000 title description 4
239000003795 chemical substances by application Substances 0.000 claims abstract description 58
239000000203 mixture Substances 0.000 claims abstract description 41
KCXVZYZYPLLWCC-UHFFFAOYSA-N EDTA Chemical compound OC(=O)CN(CC(O)=O)CCN(CC(O)=O)CC(O)=O KCXVZYZYPLLWCC-UHFFFAOYSA-N 0.000 claims abstract description 38
PUZPDOWCWNUUKD-UHFFFAOYSA-M sodium fluoride Chemical compound [F-].[Na+] PUZPDOWCWNUUKD-UHFFFAOYSA-M 0.000 claims abstract description 36
230000002797 proteolythic effect Effects 0.000 claims abstract description 31
239000001509 sodium citrate Substances 0.000 claims abstract description 27
NLJMYIDDQXHKNR-UHFFFAOYSA-K sodium citrate Chemical compound O.O.[Na+].[Na+].[Na+].[O-]C(=O)CC(O)(CC([O-])=O)C([O-])=O NLJMYIDDQXHKNR-UHFFFAOYSA-K 0.000 claims abstract description 27
230000003413 degradative effect Effects 0.000 claims abstract description 21
239000011775 sodium fluoride Substances 0.000 claims abstract description 18
235000013024 sodium fluoride Nutrition 0.000 claims abstract description 18
229910021538 borax Inorganic materials 0.000 claims abstract description 16
235000010339 sodium tetraborate Nutrition 0.000 claims abstract description 16
BSVBQGMMJUBVOD-UHFFFAOYSA-N trisodium borate Chemical compound [Na+].[Na+].[Na+].[O-]B([O-])[O-] BSVBQGMMJUBVOD-UHFFFAOYSA-N 0.000 claims abstract description 16
239000002738 chelating agent Substances 0.000 claims abstract description 14
230000000694 effects Effects 0.000 claims abstract description 10
229910052751 metal Inorganic materials 0.000 claims abstract description 7
239000002184 metal Substances 0.000 claims abstract description 7
235000011083 sodium citrates Nutrition 0.000 claims abstract description 5
210000002700 urine Anatomy 0.000 claims description 51
230000005764 inhibitory process Effects 0.000 claims description 10
239000011541 reaction mixture Substances 0.000 claims description 10
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230000002708 enhancing effect Effects 0.000 claims description 5
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108010053770 Deoxyribonucleases Proteins 0.000 claims 2
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108010083644 Ribonucleases Proteins 0.000 claims 2
229910021654 trace metal Inorganic materials 0.000 claims 2
101710163270 Nuclease Proteins 0.000 abstract description 9
150000002739 metals Chemical class 0.000 abstract description 6
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238000003199 nucleic acid amplification method Methods 0.000 description 24
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239000000654 additive Substances 0.000 description 14
239000000126 substance Substances 0.000 description 14
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230000000996 additive effect Effects 0.000 description 11
IAZDPXIOMUYVGZ-UHFFFAOYSA-N Dimethylsulphoxide Chemical compound CS(C)=O IAZDPXIOMUYVGZ-UHFFFAOYSA-N 0.000 description 10
230000009089 cytolysis Effects 0.000 description 10
PEDCQBHIVMGVHV-UHFFFAOYSA-N Glycerine Chemical compound OCC(O)CO PEDCQBHIVMGVHV-UHFFFAOYSA-N 0.000 description 9
239000013612 plasmid Substances 0.000 description 9
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230000015556 catabolic process Effects 0.000 description 6
KRKNYBCHXYNGOX-UHFFFAOYSA-N citric acid Chemical compound OC(=O)CC(O)(C(O)=O)CC(O)=O KRKNYBCHXYNGOX-UHFFFAOYSA-N 0.000 description 6
238000006731 degradation reaction Methods 0.000 description 6
DEFVIWRASFVYLL-UHFFFAOYSA-N ethylene glycol bis(2-aminoethyl)tetraacetic acid Chemical compound OC(=O)CN(CC(O)=O)CCOCCOCCN(CC(O)=O)CC(O)=O DEFVIWRASFVYLL-UHFFFAOYSA-N 0.000 description 6
SZVJSHCCFOBDDC-UHFFFAOYSA-N ferrosoferric oxide Chemical compound O=[Fe]O[Fe]O[Fe]=O SZVJSHCCFOBDDC-UHFFFAOYSA-N 0.000 description 6
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229930006000 Sucrose Natural products 0.000 description 5
CZMRCDWAGMRECN-UGDNZRGBSA-N Sucrose Chemical compound O[C@H]1[C@H](O)[C@@H](CO)O[C@@]1(CO)O[C@@H]1[C@H](O)[C@@H](O)[C@H](O)[C@@H](CO)O1 CZMRCDWAGMRECN-UGDNZRGBSA-N 0.000 description 5
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DGAQECJNVWCQMB-PUAWFVPOSA-M Ilexoside XXIX Chemical compound C[C@@H]1CC[C@@]2(CC[C@@]3(C(=CC[C@H]4[C@]3(CC[C@@H]5[C@@]4(CC[C@@H](C5(C)C)OS(=O)(=O)[O-])C)C)[C@@H]2[C@]1(C)O)C)C(=O)O[C@H]6[C@@H]([C@H]([C@@H]([C@H](O6)CO)O)O)O.[Na+] DGAQECJNVWCQMB-PUAWFVPOSA-M 0.000 description 4
XEEYBQQBJWHFJM-UHFFFAOYSA-N Iron Chemical compound [Fe] XEEYBQQBJWHFJM-UHFFFAOYSA-N 0.000 description 4
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HLBBKKJFGFRGMU-UHFFFAOYSA-M sodium formate Chemical compound [Na+].[O-]C=O HLBBKKJFGFRGMU-UHFFFAOYSA-M 0.000 description 4
235000019254 sodium formate Nutrition 0.000 description 4
239000003381 stabilizer Substances 0.000 description 4
-1 2-aminophenoxy Chemical group 0.000 description 3
FSVCELGFZIQNCK-UHFFFAOYSA-N N,N-bis(2-hydroxyethyl)glycine Chemical compound OCCN(CCO)CC(O)=O FSVCELGFZIQNCK-UHFFFAOYSA-N 0.000 description 3
239000007998 bicine buffer Substances 0.000 description 3
230000003247 decreasing effect Effects 0.000 description 3
238000001514 detection method Methods 0.000 description 3
QLBHNVFOQLIYTH-UHFFFAOYSA-L dipotassium;2-[2-[bis(carboxymethyl)amino]ethyl-(carboxylatomethyl)amino]acetate Chemical compound [K+].[K+].OC(=O)CN(CC([O-])=O)CCN(CC(O)=O)CC([O-])=O QLBHNVFOQLIYTH-UHFFFAOYSA-L 0.000 description 3
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IVLXQGJVBGMLRR-UHFFFAOYSA-N 2-aminoacetic acid;hydron;chloride Chemical compound Cl.NCC(O)=O IVLXQGJVBGMLRR-UHFFFAOYSA-N 0.000 description 2
BTBUEUYNUDRHOZ-UHFFFAOYSA-N Borate Chemical compound [O-]B([O-])[O-] BTBUEUYNUDRHOZ-UHFFFAOYSA-N 0.000 description 2
KRKNYBCHXYNGOX-UHFFFAOYSA-K Citrate Chemical compound [O-]C(=O)CC(O)(CC([O-])=O)C([O-])=O KRKNYBCHXYNGOX-UHFFFAOYSA-K 0.000 description 2
CHJJGSNFBQVOTG-UHFFFAOYSA-N N-methyl-guanidine Natural products CNC(N)=N CHJJGSNFBQVOTG-UHFFFAOYSA-N 0.000 description 2
241000588653 Neisseria Species 0.000 description 2
206010036790 Productive cough Diseases 0.000 description 2
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XSQUKJJJFZCRTK-UHFFFAOYSA-N Urea Chemical compound NC(N)=O XSQUKJJJFZCRTK-UHFFFAOYSA-N 0.000 description 2
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230000002411 adverse Effects 0.000 description 2
210000001175 cerebrospinal fluid Anatomy 0.000 description 2
SWSQBOPZIKWTGO-UHFFFAOYSA-N dimethylaminoamidine Natural products CN(C)C(N)=N SWSQBOPZIKWTGO-UHFFFAOYSA-N 0.000 description 2
238000006073 displacement reaction Methods 0.000 description 2
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VGTPCRGMBIAPIM-UHFFFAOYSA-M sodium thiocyanate Chemical compound [Na+].[S-]C#N VGTPCRGMBIAPIM-UHFFFAOYSA-M 0.000 description 2
210000003802 sputum Anatomy 0.000 description 2
208000024794 sputum Diseases 0.000 description 2
LWIHDJKSTIGBAC-UHFFFAOYSA-K tripotassium phosphate Chemical compound [K+].[K+].[K+].[O-]P([O-])([O-])=O LWIHDJKSTIGBAC-UHFFFAOYSA-K 0.000 description 2
108010039627 Aprotinin Proteins 0.000 description 1
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FYYHWMGAXLPEAU-UHFFFAOYSA-N Magnesium Chemical compound [Mg] FYYHWMGAXLPEAU-UHFFFAOYSA-N 0.000 description 1
PWHULOQIROXLJO-UHFFFAOYSA-N Manganese Chemical compound [Mn] PWHULOQIROXLJO-UHFFFAOYSA-N 0.000 description 1
241000187479 Mycobacterium tuberculosis Species 0.000 description 1
108091028043 Nucleic acid sequence Proteins 0.000 description 1
108090000783 Renin Proteins 0.000 description 1
102100028255 Renin Human genes 0.000 description 1
ABBQHOQBGMUPJH-UHFFFAOYSA-M Sodium salicylate Chemical compound [Na+].OC1=CC=CC=C1C([O-])=O ABBQHOQBGMUPJH-UHFFFAOYSA-M 0.000 description 1
108090000631 Trypsin Proteins 0.000 description 1
102000004142 Trypsin Human genes 0.000 description 1
HCHKCACWOHOZIP-UHFFFAOYSA-N Zinc Chemical compound [Zn] HCHKCACWOHOZIP-UHFFFAOYSA-N 0.000 description 1
230000001133 acceleration Effects 0.000 description 1
239000012491 analyte Substances 0.000 description 1
230000000844 anti-bacterial effect Effects 0.000 description 1
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238000003556 assay Methods 0.000 description 1
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239000003112 inhibitor Substances 0.000 description 1
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238000012423 maintenance Methods 0.000 description 1
229910052748 manganese Inorganic materials 0.000 description 1
239000011572 manganese Substances 0.000 description 1
230000001404 mediated effect Effects 0.000 description 1
238000007899 nucleic acid hybridization Methods 0.000 description 1
229950000964 pepstatin Drugs 0.000 description 1
108010091212 pepstatin Proteins 0.000 description 1
FAXGPCHRFPCXOO-LXTPJMTPSA-N pepstatin A Chemical compound OC(=O)C[C@H](O)[C@H](CC(C)C)NC(=O)[C@H](C)NC(=O)C[C@H](O)[C@H](CC(C)C)NC(=O)[C@H](C(C)C)NC(=O)[C@H](C(C)C)NC(=O)CC(C)C FAXGPCHRFPCXOO-LXTPJMTPSA-N 0.000 description 1
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238000005057 refrigeration Methods 0.000 description 1
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230000007017 scission Effects 0.000 description 1
238000000926 separation method Methods 0.000 description 1
210000002966 serum Anatomy 0.000 description 1
BAZAXWOYCMUHIX-UHFFFAOYSA-M sodium perchlorate Chemical compound [Na+].[O-]Cl(=O)(=O)=O BAZAXWOYCMUHIX-UHFFFAOYSA-M 0.000 description 1
229910001488 sodium perchlorate Inorganic materials 0.000 description 1
229960004025 sodium salicylate Drugs 0.000 description 1
239000002689 soil Substances 0.000 description 1
239000007787 solid Substances 0.000 description 1
230000006641 stabilisation Effects 0.000 description 1
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239000006228 supernatant Substances 0.000 description 1
238000013518 transcription Methods 0.000 description 1
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HRXKRNGNAMMEHJ-UHFFFAOYSA-K trisodium citrate Chemical compound [Na+].[Na+].[Na+].[O-]C(=O)CC(O)(CC([O-])=O)C([O-])=O HRXKRNGNAMMEHJ-UHFFFAOYSA-K 0.000 description 1
239000012588 trypsin Substances 0.000 description 1
238000010792 warming Methods 0.000 description 1
XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 1
239000011701 zinc Substances 0.000 description 1
229910052725 zinc Inorganic materials 0.000 description 1

Landscapes

Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Investigating Or Analysing Biological Materials (AREA)

CA 2437473 2002-08-20 2003-08-18 Methode pour conserver des cellules et des cibles d'acide nucleique Abandoned CA2437473A1 (fr)

Applications Claiming Priority (2)

Application Number	Priority Date	Filing Date	Title
US10/225,065 US7029840B2 (en)	2000-11-15	2002-08-20	Method for preservation of cells and nucleic acid targets
US10/225,065		2002-08-20

Publications (1)

Publication Number	Publication Date
CA2437473A1 true CA2437473A1 (fr)	2004-02-20

Family

ID=31946293

Family Applications (1)

Application Number	Title	Priority Date	Filing Date
CA 2437473 Abandoned CA2437473A1 (fr)	2002-08-20	2003-08-18	Methode pour conserver des cellules et des cibles d'acide nucleique

Country Status (2)

Country	Link
JP (1)	JP2004159648A (fr)
CA (1)	CA2437473A1 (fr)

Families Citing this family (2)

* Cited by examiner, † Cited by third party
Publication number	Priority date	Publication date	Assignee	Title
JP2011250757A (ja)	2010-06-03	2011-12-15	Olympus Corp	生体試料中の核酸検出方法
WO2012008581A1 (fr) *	2010-07-16	2012-01-19	日本碍子株式会社	Support qui porte des informations d'identification pour l'identification d'un sujet d'identification et son utilisation

2003
- 2003-08-18 CA CA 2437473 patent/CA2437473A1/fr not_active Abandoned
- 2003-08-20 JP JP2003296742A patent/JP2004159648A/ja active Pending

Also Published As

Publication number	Publication date
JP2004159648A (ja)	2004-06-10

Legal Events

Date	Code	Title	Description
2008-08-11	EEER	Examination request
2012-06-20	FZDE	Dead

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US7029840B2 (en)	2006-04-18	Method for preservation of cells and nucleic acid targets
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