CA2440183A1 - Determination of analytes by means of fluorescence correlation spectroscopy - Google Patents

Determination of analytes by means of fluorescence correlation spectroscopy Download PDF

Info

Publication number
CA2440183A1
CA2440183A1 CA002440183A CA2440183A CA2440183A1 CA 2440183 A1 CA2440183 A1 CA 2440183A1 CA 002440183 A CA002440183 A CA 002440183A CA 2440183 A CA2440183 A CA 2440183A CA 2440183 A1 CA2440183 A1 CA 2440183A1
Authority
CA
Canada
Prior art keywords
support
luminescence
determination
matrix
measurement
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Abandoned
Application number
CA002440183A
Other languages
English (en)
French (fr)
Inventor
Rudolf Rigler
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Gnothis Holding SA
Original Assignee
Individual
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from DE10111420A external-priority patent/DE10111420A1/de
Application filed by Individual filed Critical Individual
Publication of CA2440183A1 publication Critical patent/CA2440183A1/en
Abandoned legal-status Critical Current

Links

Classifications

    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/508—Rigid containers without fluid transport within
    • B01L3/5085—Rigid containers without fluid transport within for multiple samples, e.g. microtitration plates
    • B01L3/50851—Rigid containers without fluid transport within for multiple samples, e.g. microtitration plates specially adapted for heating or cooling samples
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64—Fluorescence; Phosphorescence
    • G01N21/6408—Fluorescence; Phosphorescence with measurement of decay time, time resolved fluorescence
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64—Fluorescence; Phosphorescence
    • G01N21/645—Specially adapted constructive features of fluorimeters
    • G01N21/6452—Individual samples arranged in a regular 2D-array, e.g. multiwell plates
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64—Fluorescence; Phosphorescence
    • G01N21/6428—Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes"
    • G01N2021/6439—Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes" with indicators, stains, dyes, tags, labels, marks
    • G01N2021/6441—Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes" with indicators, stains, dyes, tags, labels, marks with two or more labels
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64—Fluorescence; Phosphorescence
    • G01N21/645—Specially adapted constructive features of fluorimeters
    • G01N2021/6463—Optics
    • G01N2021/6478—Special lenses
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64—Fluorescence; Phosphorescence
    • G01N21/645—Specially adapted constructive features of fluorimeters
    • G01N21/6456—Spatial resolved fluorescence measurements; Imaging
    • G01N21/6458—Fluorescence microscopy

Landscapes

  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • General Health & Medical Sciences (AREA)
  • Analytical Chemistry (AREA)
  • General Physics & Mathematics (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Biochemistry (AREA)
  • Physics & Mathematics (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • Immunology (AREA)
  • Pathology (AREA)
  • Hematology (AREA)
  • Clinical Laboratory Science (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Investigating, Analyzing Materials By Fluorescence Or Luminescence (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Investigating Or Analysing Materials By The Use Of Chemical Reactions (AREA)
CA002440183A 2001-03-09 2002-03-08 Determination of analytes by means of fluorescence correlation spectroscopy Abandoned CA2440183A1 (en)

Applications Claiming Priority (5)

Application Number Priority Date Filing Date Title
DE10111420.6 2001-03-09
DE10111420A DE10111420A1 (de) 2001-03-09 2001-03-09 Bestimmung von Analyten durch Fluoreszenz-Korrelationsspektroskopie
US33794201P 2001-12-06 2001-12-06
US60/337,942 2001-12-06
PCT/EP2002/002582 WO2002073172A2 (de) 2001-03-09 2002-03-08 Bestimmung von analyten durch fluoreszenz-korrelationsspektroskopie

Publications (1)

Publication Number Publication Date
CA2440183A1 true CA2440183A1 (en) 2002-09-19

Family

ID=26008727

Family Applications (1)

Application Number Title Priority Date Filing Date
CA002440183A Abandoned CA2440183A1 (en) 2001-03-09 2002-03-08 Determination of analytes by means of fluorescence correlation spectroscopy

Country Status (5)

Country Link
EP (1) EP1373871A2 (de)
JP (1) JP2004527742A (de)
AU (1) AU2002256658A1 (de)
CA (1) CA2440183A1 (de)
WO (1) WO2002073172A2 (de)

Family Cites Families (12)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5252294A (en) * 1988-06-01 1993-10-12 Messerschmitt-Bolkow-Blohm Gmbh Micromechanical structure
DE4030699C1 (de) * 1990-09-28 1991-10-10 Bruker Analytische Messtechnik Gmbh, 7512 Rheinstetten, De
EP0679251B1 (de) * 1993-01-18 1998-04-08 Evotec BioSystems GmbH Verfahren und vorrichtung zur bewertung der fitness von biopolymeren
CA2159830C (en) * 1994-04-29 2001-07-03 Applied Biosystems, Llc System for real time detection of nucleic acid amplification products
DE19533092A1 (de) * 1995-09-07 1997-03-13 Basf Ag Vorrichtung zur parallelisierten Zweiphotonen-Fluoreszenz-Korrelations-Spektroskopie (TPA-FCS) und deren Verwendung zum Wirkstoff-Screening
EP2332957B1 (de) * 1996-02-09 2015-04-08 Cornell Research Foundation, Inc. Detektion von Unterschieden in der Sequenz von Nukleinsäuren mittels der Ligasedetektionsreaktion und adressierbaren Arrays
DE19739120A1 (de) * 1997-09-06 1999-03-11 Univ Schiller Jena Verfahren zur Bestimmung der Antioxidanzkapazität von Proben
DE19748211A1 (de) * 1997-10-31 1999-05-06 Zeiss Carl Fa Optisches Array-System und Reader für Mikrotiterplatten
EP0963790A3 (de) * 1998-06-09 2000-08-16 Peter J. Krauser Mikrotiterplatte
JP2000166599A (ja) * 1998-12-11 2000-06-20 Masataka Kaneshiro 標的核酸の定量分析方法
WO2000066985A1 (en) * 1999-04-29 2000-11-09 Evotec Biosystems Ag A method of characterizing fluorescent molecules or other particles using generating functions
AU4307700A (en) * 1999-06-26 2001-01-31 Packard Instrument Company, Inc. Microplate reader

Also Published As

Publication number Publication date
WO2002073172A2 (de) 2002-09-19
WO2002073172A3 (de) 2003-10-30
EP1373871A2 (de) 2004-01-02
AU2002256658A1 (en) 2002-09-24
JP2004527742A (ja) 2004-09-09

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