CA2444480A1 - Methodes et compositions pour produire des peptides recombinants - Google Patents

Methodes et compositions pour produire des peptides recombinants Download PDF

Info

Publication number
CA2444480A1
CA2444480A1 CA002444480A CA2444480A CA2444480A1 CA 2444480 A1 CA2444480 A1 CA 2444480A1 CA 002444480 A CA002444480 A CA 002444480A CA 2444480 A CA2444480 A CA 2444480A CA 2444480 A1 CA2444480 A1 CA 2444480A1
Authority
CA
Canada
Prior art keywords
protein
solution
solubilization
polypeptide
stabilizing compound
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Granted
Application number
CA002444480A
Other languages
English (en)
Other versions
CA2444480C (fr
Inventor
Lucia Irene Gonzalez-Villasenor
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Individual
Original Assignee
Individual
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Individual filed Critical Individual
Publication of CA2444480A1 publication Critical patent/CA2444480A1/fr
Application granted granted Critical
Publication of CA2444480C publication Critical patent/CA2444480C/fr
Anticipated expiration legal-status Critical
Expired - Fee Related legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/46—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates
    • C07K14/47—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates from mammals
    • C07K14/4701—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates from mammals not used
    • C07K14/4716—Muscle proteins, e.g. myosin, actin
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/107—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length by chemical modification of precursor peptides
    • C07K1/113—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length by chemical modification of precursor peptides without change of the primary structure
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P21/00—Preparation of peptides or proteins
    • C12P21/02—Preparation of peptides or proteins having a known sequence of two or more amino acids, e.g. glutathione

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • Zoology (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Medicinal Chemistry (AREA)
  • Wood Science & Technology (AREA)
  • General Chemical & Material Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Engineering & Computer Science (AREA)
  • Biophysics (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Toxicology (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Analytical Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Peptides Or Proteins (AREA)

Abstract

L'invention concerne un procédé de solubilisation et de récupération, dans une forme bioactive et isolée dont la configuration est maintenue à l'état natif, un peptide cible provenant d'un organisme hôte dans lequel le polypeptide hétérologue est sous forme insoluble. De manière générale, ce procédé consiste à : (I) dissoudre la cellule hôte pour obtenir un lysat, (II) récupérer le précipité de lysat qui renferme le polypeptide, (III) remettre en suspension le précipité de lysat dans une solution de solubilisation non tamponnée exempte d'agent dénaturant pour obtenir une préparation de solubilisation qui contient à la fois l'hydroxyde de sodium entre environ 8 et environ 10 mM et un peptide cible entre environ 1 et environ 4 mg de peptide par ml de solution de solubilisation, la préparation de solubilisation obtenue présentant un pH compris entre environ 9 et environ 11, 2; et enfin, (IV) récupérer le surnageant dans la préparation de solubilisation qui contient le peptide cible non dénaturé. Des composés stabilisants ainsi que des détergents peuvent être éventuellement utilisés. Par ailleurs, l'invention concerne des protéines insolubles isolées sous forme bioactive dont la configuration est à l'état natif.
CA2444480A 2001-02-23 2002-02-22 Methodes et compositions pour produire des peptides recombinants Expired - Fee Related CA2444480C (fr)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US27083901P 2001-02-23 2001-02-23
US60/270,839 2001-02-23
PCT/US2002/005256 WO2003102013A2 (fr) 2001-02-23 2002-02-22 Procedes et compositions de production de peptides recombinants

Publications (2)

Publication Number Publication Date
CA2444480A1 true CA2444480A1 (fr) 2002-08-23
CA2444480C CA2444480C (fr) 2014-05-27

Family

ID=29711700

Family Applications (1)

Application Number Title Priority Date Filing Date
CA2444480A Expired - Fee Related CA2444480C (fr) 2001-02-23 2002-02-22 Methodes et compositions pour produire des peptides recombinants

Country Status (3)

Country Link
US (1) US6936437B2 (fr)
CA (1) CA2444480C (fr)
WO (1) WO2003102013A2 (fr)

Families Citing this family (17)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US9274101B2 (en) * 2001-04-20 2016-03-01 Biolog, Inc. Methods and kits for obtaining a metabolic profile of living animal cells
US20030162164A1 (en) * 2001-04-20 2003-08-28 Biolog, Inc. Comparative phenotype analysis of cells, including testing of biologically active compounds
SI21273A (sl) * 2002-07-31 2004-02-29 LEK farmacevtska dru�ba d.d. Priprava inkluzijskih teles z visokim deležem pravilno zvitega prekurzorja heterolognega proteina
US20120178910A1 (en) * 2009-09-23 2012-07-12 Medarex, Inc. Cation exchange chromatography (methods)
WO2011100369A2 (fr) * 2010-02-09 2011-08-18 The Trustees Of Columbia University In The City Of New York Procédés de modification d'expression et de solubilité de polypeptides
US20130171128A1 (en) * 2010-03-02 2013-07-04 Amgen Inc. Reducing viscosity of pharmaceutical formulations
JP6081908B2 (ja) * 2010-03-31 2017-02-15 アーエムシルク ゲーエムベーハー 不溶性の標的タンパク質の分離
WO2011131491A1 (fr) * 2010-04-22 2011-10-27 Scil Proteins Gmbh Procédé de production d'une protéine recombinante soluble
US20130078275A1 (en) * 2010-05-25 2013-03-28 The Board Of Trustees Of The University Of Illinois Novel systems, vectors, and methods for delivery of biomolecules to eukaryotic cells
CA2815342A1 (fr) * 2010-10-20 2012-04-26 Medimmune, Llc Methodes de traitement de corps d'inclusion
US9512181B2 (en) 2011-05-27 2016-12-06 Tetragenetics, Inc. Fusion proteins of ciliate granule lattice proteins, granular protein particles thereof, and uses therefor
US20150056653A1 (en) * 2011-07-29 2015-02-26 Georgia State University Research Foundation, Inc. Protein production method
AU2012326170B2 (en) * 2011-10-18 2016-12-22 Coherus Biosciences, Inc. Etanercept formulations stabilized with metal ions
US10065987B2 (en) 2013-02-12 2018-09-04 Bristol-Myers Squibb Company High pH protein refolding methods
US9982012B2 (en) 2013-03-29 2018-05-29 Dr. Reddy's Laboratories Limited Refolding of granulocyte colony stimulating factor
CN106243186B (zh) * 2015-06-15 2020-12-25 张鹏 可独立用于蛋白质复性或作为蛋白质复性前导操作的循环运作方法
US20220387551A1 (en) * 2019-10-20 2022-12-08 Sl Technology, Inc. Methods using sonication to increase bioactivity of soy peptides

Family Cites Families (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5523215A (en) 1985-03-28 1996-06-04 Chiron Corporation Enhanced purification and expression of insoluble recombinant proteins
US4748234A (en) * 1985-06-26 1988-05-31 Cetus Corporation Process for recovering refractile bodies containing heterologous proteins from microbial hosts
US4677196A (en) 1985-09-06 1987-06-30 International Minerals & Chemical Corp. Purification and activation of proteins from insoluble inclusion bodies
US5530100A (en) * 1990-05-07 1996-06-25 Rhone-Poulenc Rorer Pharmaceuticals Inc. Methods for purification of recombinantly produced proteins
CA2130280C (fr) * 1993-08-24 1999-08-31 Lilian Lee Methode de purification de la troponine i cardiaque
US5834210A (en) * 1997-05-23 1998-11-10 Spectral Diagnostics, Inc. Stable troponin subunits and complexes

Also Published As

Publication number Publication date
US20030166062A1 (en) 2003-09-04
WO2003102013A3 (fr) 2005-03-03
WO2003102013A2 (fr) 2003-12-11
CA2444480C (fr) 2014-05-27
US6936437B2 (en) 2005-08-30

Similar Documents

Publication Publication Date Title
CA2444480C (fr) Methodes et compositions pour produire des peptides recombinants
JPS6091986A (ja) ポリペプチドの産生を増強する発現用プラスミド及び該プラスミドを含む形質転換宿主細胞
US11697803B2 (en) Method of protein purification from E.coli
US20160060291A1 (en) Process for renaturation of polypeptides
WO1996007744A1 (fr) Procede de production amelioree des facteurs de croissance proches de l'insuline
US7189811B2 (en) Process for solubilization of recombinant proteins expressed as inclusion body
US20050227920A1 (en) Methods for production of recombinant vascular endothelial cell growth inhibitor
US6916914B1 (en) Purification of somatotropin from transformed microorganisms
JPH08502963A (ja) ソマトトロピン修飾物
US6605706B1 (en) Method for producing a correctly folded, biological active recombinant protein
EP0482124B1 (fr) Analogues de somatotrophine
US20120214965A1 (en) Glargine proinsulin and methods of producing glargine insulin analogs therefrom
EP0282318A2 (fr) Analogue de l'hormone de croissance bovine
US20160024168A1 (en) Aspart proinsulin compositions and methods of producing aspart insulin analogs
US6734164B2 (en) Process for the stabilization of proteins in complex mixtures during their storage in aqueous solvents
US20130210716A1 (en) Lis-pro proinsulin compositions and methods of producing lis-pro insulin analogs therefrom
EP1592703A1 (fr) Methode d'isolation de proteines dans des conditions anoxiques
US20110237509A1 (en) hGH AND METHODS FOR PREPARING hGH
CN1726281B (zh) 在原核宿主中生产il-21
TR2025011974A1 (tr) Yeni̇ varyant i̇nsuli̇nler ve bunlarin hazirlanma yöntemi̇
JPH09262093A (ja) 蛋白質の活性化方法
Wilhelmi The Comparative Biochemistry Of Pituitary Growth Hormone: The Seventh Edsel B. Ford Lecture
Strainienė Studies of the refolding processes of recombinant growth hormones
HK1023781B (en) Method for producing a correctly folded, biological active recombinant protein
JPH09121886A (ja) ニューロトロフィン３類の製造方法

Legal Events

Date Code Title Description
EEER Examination request
MKLA Lapsed

Effective date: 20190222