CA2465594A1 - Compositions de microparticules marquees et procedes associes - Google Patents
Compositions de microparticules marquees et procedes associes Download PDFInfo
- Publication number
- CA2465594A1 CA2465594A1 CA002465594A CA2465594A CA2465594A1 CA 2465594 A1 CA2465594 A1 CA 2465594A1 CA 002465594 A CA002465594 A CA 002465594A CA 2465594 A CA2465594 A CA 2465594A CA 2465594 A1 CA2465594 A1 CA 2465594A1
- Authority
- CA
- Canada
- Prior art keywords
- composition
- molecular tags
- molecular
- binding
- separation
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
- 239000000203 mixture Substances 0.000 title claims abstract 60
- 239000011859 microparticle Substances 0.000 title claims abstract 24
- 238000000034 method Methods 0.000 title claims abstract 4
- 239000012491 analyte Substances 0.000 claims abstract 10
- 238000003776 cleavage reaction Methods 0.000 claims abstract 6
- 230000007017 scission Effects 0.000 claims abstract 6
- 238000000926 separation method Methods 0.000 claims 20
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 claims 9
- 230000003287 optical effect Effects 0.000 claims 9
- 229910052760 oxygen Inorganic materials 0.000 claims 9
- 239000001301 oxygen Substances 0.000 claims 9
- IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 claims 8
- 239000003504 photosensitizing agent Substances 0.000 claims 6
- 230000003213 activating effect Effects 0.000 claims 5
- 210000004027 cell Anatomy 0.000 claims 5
- 230000005281 excited state Effects 0.000 claims 5
- GNBHRKFJIUUOQI-UHFFFAOYSA-N fluorescein Chemical compound O1C(=O)C2=CC=CC=C2C21C1=CC=C(O)C=C1OC1=CC(O)=CC=C21 GNBHRKFJIUUOQI-UHFFFAOYSA-N 0.000 claims 5
- ZOXJGFHDIHLPTG-UHFFFAOYSA-N Boron Chemical compound [B] ZOXJGFHDIHLPTG-UHFFFAOYSA-N 0.000 claims 4
- OKTJSMMVPCPJKN-UHFFFAOYSA-N Carbon Chemical compound [C] OKTJSMMVPCPJKN-UHFFFAOYSA-N 0.000 claims 4
- OAICVXFJPJFONN-UHFFFAOYSA-N Phosphorus Chemical compound [P] OAICVXFJPJFONN-UHFFFAOYSA-N 0.000 claims 4
- 239000004952 Polyamide Substances 0.000 claims 4
- NINIDFKCEFEMDL-UHFFFAOYSA-N Sulfur Chemical compound [S] NINIDFKCEFEMDL-UHFFFAOYSA-N 0.000 claims 4
- 150000001336 alkenes Chemical class 0.000 claims 4
- 125000004429 atom Chemical group 0.000 claims 4
- 229920001400 block copolymer Polymers 0.000 claims 4
- 229910052796 boron Inorganic materials 0.000 claims 4
- 229910052799 carbon Inorganic materials 0.000 claims 4
- 238000013375 chromatographic separation Methods 0.000 claims 4
- 238000001514 detection method Methods 0.000 claims 4
- 239000007850 fluorescent dye Substances 0.000 claims 4
- 229910052739 hydrogen Inorganic materials 0.000 claims 4
- 239000001257 hydrogen Substances 0.000 claims 4
- 125000004435 hydrogen atom Chemical class [H]* 0.000 claims 4
- 150000002460 imidazoles Chemical class 0.000 claims 4
- 229910052757 nitrogen Inorganic materials 0.000 claims 4
- 229920001542 oligosaccharide Polymers 0.000 claims 4
- 150000002482 oligosaccharides Chemical class 0.000 claims 4
- 150000002894 organic compounds Chemical class 0.000 claims 4
- 150000002916 oxazoles Chemical class 0.000 claims 4
- 229910052698 phosphorus Inorganic materials 0.000 claims 4
- 239000011574 phosphorus Substances 0.000 claims 4
- 229920002647 polyamide Polymers 0.000 claims 4
- 229920000728 polyester Polymers 0.000 claims 4
- 229920000570 polyether Polymers 0.000 claims 4
- 229920000642 polymer Polymers 0.000 claims 4
- 229920001184 polypeptide Polymers 0.000 claims 4
- 229920002635 polyurethane Polymers 0.000 claims 4
- 239000004814 polyurethane Substances 0.000 claims 4
- 102000004196 processed proteins & peptides Human genes 0.000 claims 4
- 108090000765 processed proteins & peptides Proteins 0.000 claims 4
- 150000003346 selenoethers Chemical class 0.000 claims 4
- 229910052717 sulfur Inorganic materials 0.000 claims 4
- 239000011593 sulfur Substances 0.000 claims 4
- 150000003557 thiazoles Chemical class 0.000 claims 4
- 150000003568 thioethers Chemical class 0.000 claims 4
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims 4
- BZTDTCNHAFUJOG-UHFFFAOYSA-N 6-carboxyfluorescein Chemical compound C12=CC=C(O)C=C2OC2=CC(O)=CC=C2C11OC(=O)C2=CC=C(C(=O)O)C=C21 BZTDTCNHAFUJOG-UHFFFAOYSA-N 0.000 claims 2
- 108091034117 Oligonucleotide Proteins 0.000 claims 2
- 239000012528 membrane Substances 0.000 claims 2
- NJYVEMPWNAYQQN-UHFFFAOYSA-N 5-carboxyfluorescein Chemical compound C12=CC=C(O)C=C2OC2=CC(O)=CC=C2C21OC(=O)C1=CC(C(=O)O)=CC=C21 NJYVEMPWNAYQQN-UHFFFAOYSA-N 0.000 claims 1
- JLCPHMBAVCMARE-UHFFFAOYSA-N [3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-[[3-[[3-[[3-[[3-[[3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-hydroxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methyl [5-(6-aminopurin-9-yl)-2-(hydroxymethyl)oxolan-3-yl] hydrogen phosphate Polymers Cc1cn(C2CC(OP(O)(=O)OCC3OC(CC3OP(O)(=O)OCC3OC(CC3O)n3cnc4c3nc(N)[nH]c4=O)n3cnc4c3nc(N)[nH]c4=O)C(COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3CO)n3cnc4c(N)ncnc34)n3ccc(N)nc3=O)n3cnc4c(N)ncnc34)n3ccc(N)nc3=O)n3ccc(N)nc3=O)n3ccc(N)nc3=O)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cc(C)c(=O)[nH]c3=O)n3cc(C)c(=O)[nH]c3=O)n3ccc(N)nc3=O)n3cc(C)c(=O)[nH]c3=O)n3cnc4c3nc(N)[nH]c4=O)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)O2)c(=O)[nH]c1=O JLCPHMBAVCMARE-UHFFFAOYSA-N 0.000 claims 1
- 239000003446 ligand Substances 0.000 claims 1
- 102000006240 membrane receptors Human genes 0.000 claims 1
- 108020004084 membrane receptors Proteins 0.000 claims 1
- 108091033319 polynucleotide Proteins 0.000 claims 1
- 102000040430 polynucleotide Human genes 0.000 claims 1
- 239000002157 polynucleotide Substances 0.000 claims 1
- 102000004169 proteins and genes Human genes 0.000 claims 1
- 108090000623 proteins and genes Proteins 0.000 claims 1
- 102000005962 receptors Human genes 0.000 claims 1
- 108020003175 receptors Proteins 0.000 claims 1
- 230000004913 activation Effects 0.000 abstract 1
- 238000004458 analytical method Methods 0.000 abstract 1
- 238000003556 assay Methods 0.000 abstract 1
- 239000003153 chemical reaction reagent Substances 0.000 abstract 1
- 230000001939 inductive effect Effects 0.000 abstract 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07D—HETEROCYCLIC COMPOUNDS
- C07D495/00—Heterocyclic compounds containing in the condensed system at least one hetero ring having sulfur atoms as the only ring hetero atoms
- C07D495/02—Heterocyclic compounds containing in the condensed system at least one hetero ring having sulfur atoms as the only ring hetero atoms in which the condensed system contains two hetero rings
- C07D495/04—Ortho-condensed systems
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07D—HETEROCYCLIC COMPOUNDS
- C07D405/00—Heterocyclic compounds containing both one or more hetero rings having oxygen atoms as the only ring hetero atoms, and one or more rings having nitrogen as the only ring hetero atom
- C07D405/02—Heterocyclic compounds containing both one or more hetero rings having oxygen atoms as the only ring hetero atoms, and one or more rings having nitrogen as the only ring hetero atom containing two hetero rings
- C07D405/12—Heterocyclic compounds containing both one or more hetero rings having oxygen atoms as the only ring hetero atoms, and one or more rings having nitrogen as the only ring hetero atom containing two hetero rings linked by a chain containing hetero atoms as chain links
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07D—HETEROCYCLIC COMPOUNDS
- C07D493/00—Heterocyclic compounds containing oxygen atoms as the only ring hetero atoms in the condensed system
- C07D493/02—Heterocyclic compounds containing oxygen atoms as the only ring hetero atoms in the condensed system in which the condensed system contains two hetero rings
- C07D493/10—Spiro-condensed systems
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
- G01N33/54313—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals the carrier being characterised by its particulate form
-
- C—CHEMISTRY; METALLURGY
- C40—COMBINATORIAL TECHNOLOGY
- C40B—COMBINATORIAL CHEMISTRY; LIBRARIES, e.g. CHEMICAL LIBRARIES
- C40B40/00—Libraries per se, e.g. arrays, mixtures
Landscapes
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Health & Medical Sciences (AREA)
- Immunology (AREA)
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Urology & Nephrology (AREA)
- Molecular Biology (AREA)
- Biomedical Technology (AREA)
- Hematology (AREA)
- Physics & Mathematics (AREA)
- Biochemistry (AREA)
- Cell Biology (AREA)
- Food Science & Technology (AREA)
- Medicinal Chemistry (AREA)
- Biotechnology (AREA)
- Analytical Chemistry (AREA)
- Microbiology (AREA)
- General Health & Medical Sciences (AREA)
- General Physics & Mathematics (AREA)
- Pathology (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Solid-Sorbent Or Filter-Aiding Compositions (AREA)
- Investigating Or Analysing Materials By The Use Of Chemical Reactions (AREA)
Abstract
L'invention concerne des compositions et des procédés de détection de nombreux mélanges cibles à analyser dans un échantillon au moyen de microparticules présentant des marqueurs moléculaires qui sont fixés par des liaisons pouvant être coupées. De manière générale, un mélange d'analyse est formé et comprend un échantillon et un réactif comprenant de nombreuses microparticules dans un état permettant à des complexes stables de se former entre les fractions de liaisons sur lesdites surfaces des microparticules et lesdits mélanges à analyser. Selon un aspect de l'invention, une seconde composition de liaison est ajoutée de sorte que des complexes se forment parmi les fractions de liaison liées aux microparticules, lesdits mélanges à analyser, et les secondes fractions de liaison de la seconde composition de liaison. Lesdites secondes fractions présentent des fractions induisant le clivage fixées qui provoquent le clivage desdites liaisons et la libération des marqueurs moléculaires à la suite de l'activation. Lesdits marqueurs moléculaires libérés sont séparés et la présence et/ou la quantité des mélanges cibles à analyser sont déterminées sur la base de l'analyse des marqueurs moléculaires libérés et séparés.
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US33776801P | 2001-11-09 | 2001-11-09 | |
| US60/337,768 | 2001-11-09 | ||
| PCT/US2002/035907 WO2003042699A1 (fr) | 2001-11-09 | 2002-11-08 | Compositions de microparticules marquees et procedes associes |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CA2465594A1 true CA2465594A1 (fr) | 2003-05-22 |
| CA2465594C CA2465594C (fr) | 2012-02-07 |
Family
ID=23321917
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CA2465594A Expired - Fee Related CA2465594C (fr) | 2001-11-09 | 2002-11-08 | Compositions de microparticules marquees et procedes associes |
Country Status (4)
| Country | Link |
|---|---|
| EP (1) | EP1456657A4 (fr) |
| JP (1) | JP2005509859A (fr) |
| CA (1) | CA2465594C (fr) |
| WO (1) | WO2003042699A1 (fr) |
Families Citing this family (12)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20040229294A1 (en) | 2002-05-21 | 2004-11-18 | Po-Ying Chan-Hui | ErbB surface receptor complexes as biomarkers |
| EP1494028A1 (fr) * | 2003-07-03 | 2005-01-05 | Labsoft Diagnostics AG | Séparation immuno-magnétique des cellulles cibles spécifiques |
| US20050266578A1 (en) * | 2004-05-06 | 2005-12-01 | Gruenke Larry D | Methods and systems for detection of macrolides |
| ATE541054T1 (de) * | 2004-05-12 | 2012-01-15 | Nanosphere Inc | Bio-barcode erkennung für zielmoleküle |
| WO2007061098A1 (fr) * | 2005-11-25 | 2007-05-31 | Japan Science And Technology Agency | Analyseur et procede d’analyse |
| EP2235536A4 (fr) | 2007-12-20 | 2011-05-04 | Lab Corp America Holdings | Procédés de diagnostic du her-2 |
| WO2009149257A1 (fr) | 2008-06-04 | 2009-12-10 | The University Of Chicago | Chemistrode : dispositif micro-fluidique à base de cônes et procédé de stimulation et d'échantillonnage avec résolution chimique, spatiale et temporelle élevée |
| ES2637411T3 (es) | 2008-12-01 | 2017-10-13 | Laboratory Corporation Of America Holdings | Métodos y ensayos para medir p95 Y/O p95 en una muestra y anticuerpos específicos para p95 |
| EP2387711B1 (fr) | 2009-01-15 | 2015-04-22 | Laboratory Corporation of America Holdings | Procédés permettant de déterminer la réponse d'un patient par mesure de her-3 |
| WO2017161030A1 (fr) | 2016-03-15 | 2017-09-21 | Laboratory Corporation Of America Holdings | Procédés d'évaluation d'interactions de protéines entre des cellules |
| CN107764906A (zh) * | 2016-08-22 | 2018-03-06 | 洛阳惠中兽药有限公司 | 一种加米霉素的含量检测方法 |
| CN116626022A (zh) * | 2018-08-13 | 2023-08-22 | 上海索昕生物科技有限公司 | 一种用于化学发光分析的微球组合物及其应用 |
Family Cites Families (10)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5310687A (en) * | 1984-10-31 | 1994-05-10 | Igen, Inc. | Luminescent metal chelate labels and means for detection |
| US5935779A (en) * | 1988-11-03 | 1999-08-10 | Igen International Inc. | Methods for improved particle electrochemiluminescence assay |
| US5326692B1 (en) * | 1992-05-13 | 1996-04-30 | Molecular Probes Inc | Fluorescent microparticles with controllable enhanced stokes shift |
| US5846703A (en) * | 1990-05-15 | 1998-12-08 | Diatron Corporation | Fluorescence immunoassays using fluorescent dyes free of aggregation and serum binding |
| US5736410A (en) * | 1992-09-14 | 1998-04-07 | Sri International | Up-converting reporters for biological and other assays using laser excitation techniques |
| WO1996033411A1 (fr) * | 1995-04-18 | 1996-10-24 | Igen, Inc. | L'electrochimioluminescence de chelates de metaux de terres rares |
| JP2002504689A (ja) * | 1998-02-18 | 2002-02-12 | デイド・ベーリング・インコーポレイテッド | 複数の検体の検出において使用するための化学ルミネセンス組成物 |
| US6322980B1 (en) * | 1999-04-30 | 2001-11-27 | Aclara Biosciences, Inc. | Single nucleotide detection using degradation of a fluorescent sequence |
| US7041459B2 (en) * | 2001-05-21 | 2006-05-09 | Monogram Biosciences, Inc. | Analyzing phosphorylated proteins |
| CA2445053A1 (fr) * | 2001-05-21 | 2002-11-28 | Aclara Biosciences, Inc. | Procedes et compositions pour l'analyse de proteines |
-
2002
- 2002-11-08 JP JP2003544481A patent/JP2005509859A/ja not_active Withdrawn
- 2002-11-08 CA CA2465594A patent/CA2465594C/fr not_active Expired - Fee Related
- 2002-11-08 WO PCT/US2002/035907 patent/WO2003042699A1/fr not_active Ceased
- 2002-11-08 EP EP02778794A patent/EP1456657A4/fr not_active Withdrawn
Also Published As
| Publication number | Publication date |
|---|---|
| EP1456657A4 (fr) | 2008-03-12 |
| CA2465594C (fr) | 2012-02-07 |
| WO2003042699A1 (fr) | 2003-05-22 |
| EP1456657A1 (fr) | 2004-09-15 |
| JP2005509859A (ja) | 2005-04-14 |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| EEER | Examination request | ||
| MKLA | Lapsed |
Effective date: 20171108 |