CA2509732C - Methode de distinction et d'isolement de cellules formant des colonies et de cellules souches epitheliales de mammiferes - Google Patents

Methode de distinction et d'isolement de cellules formant des colonies et de cellules souches epitheliales de mammiferes Download PDF

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Publication number
CA2509732C
CA2509732C CA2509732A CA2509732A CA2509732C CA 2509732 C CA2509732 C CA 2509732C CA 2509732 A CA2509732 A CA 2509732A CA 2509732 A CA2509732 A CA 2509732A CA 2509732 C CA2509732 C CA 2509732C
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Canada
Prior art keywords
cells
antibodies
mammary
antibody
cd49f
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Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
CA2509732A
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English (en)
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CA2509732A1 (fr
Inventor
John Stingl
Connie J. Eaves
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British Columbia Cancer Agency BCCA
Stemcell Technologies Canada Inc
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Stemcell Technologies Inc
British Columbia Cancer Agency BCCA
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Priority to CA2509732A priority Critical patent/CA2509732C/fr
Publication of CA2509732A1 publication Critical patent/CA2509732A1/fr
Application granted granted Critical
Publication of CA2509732C publication Critical patent/CA2509732C/fr
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

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    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies
    • C07K16/18—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies against material from animals or humans
    • C07K16/28—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies against material from animals or humans against receptors, cell surface antigens or cell surface determinants
    • C07K16/2839—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies against material from animals or humans against receptors, cell surface antigens or cell surface determinants against the integrin superfamily
    • C07K16/2842—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies against material from animals or humans against receptors, cell surface antigens or cell surface determinants against the integrin superfamily against integrin beta1-subunit-containing molecules, e.g. CD29, CD49
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies
    • C07K16/18—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies against material from animals or humans
    • C07K16/28—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies against material from animals or humans against receptors, cell surface antigens or cell surface determinants
    • C07K16/289—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies against material from animals or humans against receptors, cell surface antigens or cell surface determinants against CD45
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies
    • C07K16/18—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies against material from animals or humans
    • C07K16/28—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies against material from animals or humans against receptors, cell surface antigens or cell surface determinants
    • C07K16/2896—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies against material from animals or humans against receptors, cell surface antigens or cell surface determinants against molecules with a "CD"-designation, not provided for elsewhere
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0625—Epidermal cells, skin cells; Cells of the oral mucosa
    • C12N5/0631—Mammary cells
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/569—Immunoassay; Biospecific binding assay; Materials therefor for microorganisms, e.g. protozoa, bacteria, viruses
    • G01N33/56966—Animal cells

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  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Immunology (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Medicinal Chemistry (AREA)
  • Zoology (AREA)
  • Biotechnology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Biophysics (AREA)
  • Cell Biology (AREA)
  • Wood Science & Technology (AREA)
  • Microbiology (AREA)
  • Urology & Nephrology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Hematology (AREA)
  • Dermatology (AREA)
  • Food Science & Technology (AREA)
  • Virology (AREA)
  • Physics & Mathematics (AREA)
  • Analytical Chemistry (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • General Engineering & Computer Science (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Peptides Or Proteins (AREA)

Abstract

La présente invention concerne une méthode améliorée permettant l'isolement différentiel des cellules souches mammaires de souris et de cellules souches unipotentes (CFU). La méthode comporte la déplétion des cellules non épithéliales de tissus mammaires de souris fraîchement dissociés par incubation avec une composition d'anticorps contenant des anticorps spécifiques des CD45, Ter119, CD31 et éventuellement de la CD140a. Après la formation de conjugués entre les cellules mammaires non épithéliales et les anticorps spécifiques des CD45, Ter119, CD31 et, éventuellement de la CD140a, les conjugués des cellules sont retirés et les cellules épithéliales restantes sont ensuite incubées avec une composition d'anticorps contenant des anticorps spécifiques des CD24 et CD49f. Après la formation de conjugués entre les cellules épithéliales et les anticorps spécifiques des CD24 et CD49f, les cellules souches mammaires de souris et les cellules CFU dont le luminal est restreint peuvent être isolées de façon différentielle. L'invention concerne également les trousses permettant de mener à bien cette méthode et les préparations de cellules préparées en suivant cette méthode.
CA2509732A 2005-06-10 2005-06-10 Methode de distinction et d'isolement de cellules formant des colonies et de cellules souches epitheliales de mammiferes Expired - Lifetime CA2509732C (fr)

Priority Applications (1)

Application Number Priority Date Filing Date Title
CA2509732A CA2509732C (fr) 2005-06-10 2005-06-10 Methode de distinction et d'isolement de cellules formant des colonies et de cellules souches epitheliales de mammiferes

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
CA2509732A CA2509732C (fr) 2005-06-10 2005-06-10 Methode de distinction et d'isolement de cellules formant des colonies et de cellules souches epitheliales de mammiferes

Publications (2)

Publication Number Publication Date
CA2509732A1 CA2509732A1 (fr) 2006-12-10
CA2509732C true CA2509732C (fr) 2013-03-26

Family

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Family Applications (1)

Application Number Title Priority Date Filing Date
CA2509732A Expired - Lifetime CA2509732C (fr) 2005-06-10 2005-06-10 Methode de distinction et d'isolement de cellules formant des colonies et de cellules souches epitheliales de mammiferes

Country Status (1)

Country Link
CA (1) CA2509732C (fr)

Also Published As

Publication number Publication date
CA2509732A1 (fr) 2006-12-10

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