CA2512701C - Methode amelioree pour la transcription inverse - Google Patents

Methode amelioree pour la transcription inverse Download PDF

Info

Publication number
CA2512701C
CA2512701C CA2512701A CA2512701A CA2512701C CA 2512701 C CA2512701 C CA 2512701C CA 2512701 A CA2512701 A CA 2512701A CA 2512701 A CA2512701 A CA 2512701A CA 2512701 C CA2512701 C CA 2512701C
Authority
CA
Canada
Prior art keywords
mrna
reverse transcriptase
reverse transcription
temperature
metal ions
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Fee Related
Application number
CA2512701A
Other languages
English (en)
Other versions
CA2512701A1 (fr
Inventor
Yoshihide Hayashizaki
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Dnaform KK
RIKEN
Original Assignee
Dnaform KK
RIKEN
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from JP19633196A external-priority patent/JP3709490B2/ja
Application filed by Dnaform KK, RIKEN filed Critical Dnaform KK
Priority claimed from CA002211367A external-priority patent/CA2211367C/fr
Publication of CA2512701A1 publication Critical patent/CA2512701A1/fr
Application granted granted Critical
Publication of CA2512701C publication Critical patent/CA2512701C/fr
Anticipated expiration legal-status Critical
Expired - Fee Related legal-status Critical Current

Links

Landscapes

  • Enzymes And Modification Thereof (AREA)
CA2512701A 1996-07-25 1997-07-24 Methode amelioree pour la transcription inverse Expired - Fee Related CA2512701C (fr)

Applications Claiming Priority (5)

Application Number Priority Date Filing Date Title
JP196329/1996 1996-07-25
JP196331/1996 1996-07-25
JP19633196A JP3709490B2 (ja) 1996-07-25 1996-07-25 Rnaの熱安定性の改良方法
JP19632996 1996-07-25
CA002211367A CA2211367C (fr) 1996-07-25 1997-07-24 Methode amelioree pour la transcription inverse

Related Parent Applications (1)

Application Number Title Priority Date Filing Date
CA002211367A Division CA2211367C (fr) 1996-07-25 1997-07-24 Methode amelioree pour la transcription inverse

Publications (2)

Publication Number Publication Date
CA2512701A1 CA2512701A1 (fr) 1998-01-25
CA2512701C true CA2512701C (fr) 2010-11-23

Family

ID=35311265

Family Applications (1)

Application Number Title Priority Date Filing Date
CA2512701A Expired - Fee Related CA2512701C (fr) 1996-07-25 1997-07-24 Methode amelioree pour la transcription inverse

Country Status (1)

Country Link
CA (1) CA2512701C (fr)

Also Published As

Publication number Publication date
CA2512701A1 (fr) 1998-01-25

Similar Documents

Publication Publication Date Title
CA2211367C (fr) Methode amelioree pour la transcription inverse
US6458556B1 (en) Method for enhancing enzyme activity at elevated temperature
EP1705253B1 (fr) Compositions et procédés d'amplification en chaine par polymerase-transcriptase inverse (RT-PCR)
US6300069B1 (en) Generation and amplification of nucleic acids from ribonucleic acids
US7205128B2 (en) Method for synthesis of the second strand of cDNA
US6183998B1 (en) Method for reversible modification of thermostable enzymes
US8568982B2 (en) Methods of nucleic acid synthesis using particular crowding agents and concentrations
EP3029146B1 (fr) Inhibition réduite de rt-pcr en une étape
WO1998044161A9 (fr) Compositions et procedes d'amplification en chaine par polymerase -transcriptase inverse (rt-pcr)
US9353409B2 (en) Compositions and methods for RT-PCR
JP3206894B2 (ja) 酵素を熱活性化する方法
CA2512701C (fr) Methode amelioree pour la transcription inverse
AU737546B2 (en) Stabilized aqueous nucleoside triphosphate solutions
US20160097086A1 (en) Compositions and Methods for RT-PCR
JP3536052B2 (ja) 改良された逆転写方法
JP3709490B2 (ja) Rnaの熱安定性の改良方法
EP4022083B1 (fr) Compositions et procédés pour rt-pcr multiplex et analyse génétique
JP3709471B2 (ja) 酵素を熱活性化する方法
JP2004180677A (ja) 酵素を熱活性化する方法
JP2001352974A (ja) 酵素を熱活性化する方法
JP2006051041A (ja) 酵素を熱活性化する方法

Legal Events

Date Code Title Description
EEER Examination request
MKLA Lapsed

Effective date: 20140724