CA2520982A1 - Procede pour determiner des donnees relatives a la phase du cycle cellulaire - Google Patents

Procede pour determiner des donnees relatives a la phase du cycle cellulaire Download PDF

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Publication number
CA2520982A1
CA2520982A1 CA002520982A CA2520982A CA2520982A1 CA 2520982 A1 CA2520982 A1 CA 2520982A1 CA 002520982 A CA002520982 A CA 002520982A CA 2520982 A CA2520982 A CA 2520982A CA 2520982 A1 CA2520982 A1 CA 2520982A1
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CA
Canada
Prior art keywords
cell
cells
cell cycle
image data
object area
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Abandoned
Application number
CA002520982A
Other languages
English (en)
Inventor
Nicholas Solvatore Arini
Ian David Goodyer
Alla Borisovna Zaltsman
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
GE Healthcare UK Ltd
Original Assignee
Individual
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Individual filed Critical Individual
Publication of CA2520982A1 publication Critical patent/CA2520982A1/fr
Abandoned legal-status Critical Current

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Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64—Fluorescence; Phosphorescence
    • G01N21/645—Specially adapted constructive features of fluorimeters
    • G01N21/6452—Individual samples arranged in a regular 2D-array, e.g. multiwell plates
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
    • G01N15/10—Investigating individual particles
    • G01N15/14—Optical investigation techniques, e.g. flow cytometry
    • G01N15/1468—Optical investigation techniques, e.g. flow cytometry with spatial resolution of the texture or inner structure of the particle
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64—Fluorescence; Phosphorescence
    • G01N21/6428—Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes"
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64—Fluorescence; Phosphorescence
    • G01N21/645—Specially adapted constructive features of fluorimeters
    • G01N21/6456—Spatial resolved fluorescence measurements; Imaging
    • G01N21/6458—Fluorescence microscopy
    • G—PHYSICS
    • G06—COMPUTING OR CALCULATING; COUNTING
    • G06V—IMAGE OR VIDEO RECOGNITION OR UNDERSTANDING
    • G06V20/00—Scenes; Scene-specific elements
    • G06V20/60—Type of objects
    • G06V20/69—Microscopic objects, e.g. biological cells or cellular parts
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
    • G01N15/10—Investigating individual particles
    • G01N15/14—Optical investigation techniques, e.g. flow cytometry
    • G01N2015/1477—Multiparameters
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
    • G01N15/10—Investigating individual particles
    • G01N15/14—Optical investigation techniques, e.g. flow cytometry
    • G01N2015/1493—Particle size

Landscapes

  • Health & Medical Sciences (AREA)
  • Physics & Mathematics (AREA)
  • Chemical & Material Sciences (AREA)
  • General Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Immunology (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Analytical Chemistry (AREA)
  • Biochemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • Dispersion Chemistry (AREA)
  • Optics & Photonics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • Multimedia (AREA)
  • Theoretical Computer Science (AREA)
  • Investigating, Analyzing Materials By Fluorescence Or Luminescence (AREA)
  • Investigating Or Analysing Materials By The Use Of Chemical Reactions (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

L'invention concerne un procédé permettant de déterminer des données relatives à la phase du cycle cellulaire de cellules comprenant au moins un rapporteur luminescent pouvant émettre un rayonnement, ce(s) rapporteur(s) comprenant un premier rapporteur luminescent pouvant indiquer au moins une phase du cycle cellulaire. Le procédé comporte les étapes consistant à : stocker des données de classification permettant de classer les cellules individuelles dans des phases différentes du cycle cellulaire au moyen d'un procédé de classification automatisé ; recevoir des données d'image produites par la détection du rayonnement émis par le(s) rapporteur(s) luminescent(s) ; analyser les données d'image afin d'identifier dans les données d'image des zones d'objet correspondant à des cellules individuelles ; analyser les données d'image sur la base des zones d'objet identifiées afin de déterminer, pour une cellule sélectionnée, une ou plusieurs mesures incluant la mesure d'un paramètre ayant trait à au moins une composante cytoplasmique de la cellule ; et appliquer les données de classification auxdites mesures pour classer la cellule sélectionnée dans une sous-population sélectionnée d'une pluralité de sous-populations de cellules, chaque sous-population comportant des cellules se trouvant dans une phase différente du cycle cellulaire.
CA002520982A 2003-04-02 2003-09-08 Procede pour determiner des donnees relatives a la phase du cycle cellulaire Abandoned CA2520982A1 (fr)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
GBGB0307684.1A GB0307684D0 (en) 2003-04-02 2003-04-02 Determining cell cycle phase data
GB0307684.1 2003-04-02
PCT/GB2003/003876 WO2004088573A1 (fr) 2003-04-02 2003-09-08 Procede pour determiner des donnees relatives a la phase du cycle cellulaire

Publications (1)

Publication Number Publication Date
CA2520982A1 true CA2520982A1 (fr) 2004-10-14

Family

ID=9956096

Family Applications (1)

Application Number Title Priority Date Filing Date
CA002520982A Abandoned CA2520982A1 (fr) 2003-04-02 2003-09-08 Procede pour determiner des donnees relatives a la phase du cycle cellulaire

Country Status (6)

Country Link
EP (1) EP1609111A1 (fr)
JP (1) JP2006521785A (fr)
AU (1) AU2003260795B2 (fr)
CA (1) CA2520982A1 (fr)
GB (2) GB0307684D0 (fr)
WO (1) WO2004088573A1 (fr)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP1857538B1 (fr) 2006-05-18 2016-09-14 Olympus Corporation Appareil de microscope et procédé d'observation des cellules

Families Citing this family (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US8045782B2 (en) * 2004-12-07 2011-10-25 Ge Healthcare Niagara, Inc. Method of, and apparatus and computer software for, implementing image analysis protocols
US7817841B2 (en) * 2005-11-12 2010-10-19 General Electric Company Time-lapse cell cycle analysis of unstained nuclei
JP5265092B2 (ja) * 2005-11-22 2013-08-14 オリンパス株式会社 微弱光検体の検査方法
JP5408839B2 (ja) * 2006-10-20 2014-02-05 オリンパス株式会社 細胞周期解析方法
JP2008164551A (ja) * 2006-12-29 2008-07-17 Osaka Univ 細胞の評価方法、細胞測定用システム、及び細胞測定用プログラム
ITTO20070771A1 (it) * 2007-10-29 2009-04-30 Silicon Biosystems Spa Metodo e apparato per la identificazione e manipolazione di particelle
JP6134348B2 (ja) 2015-03-31 2017-05-24 シスメックス株式会社 細胞撮像装置及び細胞撮像方法
JP6953632B2 (ja) * 2018-07-09 2021-10-27 オリンパス株式会社 光分析装置、光分析方法および学習済みモデル
JP7416134B1 (ja) * 2022-07-01 2024-01-17 Ｔｏｐｐａｎホールディングス株式会社 検出装置

Family Cites Families (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4812394A (en) * 1983-10-18 1989-03-14 University Of California Flow cytomeric measurement of DNA and incorporated nucleoside analogs
US5548661A (en) * 1991-07-12 1996-08-20 Price; Jeffrey H. Operator independent image cytometer
WO2001011341A2 (fr) * 1999-08-05 2001-02-15 Cellomics, Inc. Systeme criblage de cellules
WO2001042786A2 (fr) * 1999-12-09 2001-06-14 Cellomics, Inc. Systeme de criblage a base de cellules
GB0123856D0 (en) * 2001-10-05 2001-11-28 Amersham Pharm Biotech Uk Ltd Method for determining cell cycle position

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP1857538B1 (fr) 2006-05-18 2016-09-14 Olympus Corporation Appareil de microscope et procédé d'observation des cellules

Also Published As

Publication number Publication date
AU2003260795A1 (en) 2004-10-25
JP2006521785A (ja) 2006-09-28
EP1609111A1 (fr) 2005-12-28
WO2004088573A1 (fr) 2004-10-14
AU2003260795B2 (en) 2008-11-06
GB0307684D0 (en) 2003-05-07
GB0327651D0 (en) 2003-12-31

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Legal Events

Date Code Title Description
EEER Examination request
FZDE Discontinued