CA2545441A1 - Objets codes a reseaux de diffraction pour experiences multiplex - Google Patents
Objets codes a reseaux de diffraction pour experiences multiplex Download PDFInfo
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- CA2545441A1 CA2545441A1 CA002545441A CA2545441A CA2545441A1 CA 2545441 A1 CA2545441 A1 CA 2545441A1 CA 002545441 A CA002545441 A CA 002545441A CA 2545441 A CA2545441 A CA 2545441A CA 2545441 A1 CA2545441 A1 CA 2545441A1
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- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
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Landscapes
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- Investigating, Analyzing Materials By Fluorescence Or Luminescence (AREA)
- Investigating Or Analysing Materials By Optical Means (AREA)
Applications Claiming Priority (13)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US60/519,932 | 2003-09-14 | ||
| US51993203P | 2003-11-14 | 2003-11-14 | |
| US10/763,995 US7164533B2 (en) | 2003-01-22 | 2004-01-22 | Hybrid random bead/chip based microarray |
| US10/763,995 | 2004-01-22 | ||
| US55544904P | 2004-03-22 | 2004-03-22 | |
| US60/555,449 | 2004-03-22 | ||
| US60242704P | 2004-08-18 | 2004-08-18 | |
| US60/602,427 | 2004-08-18 | ||
| US61120504P | 2004-09-17 | 2004-09-17 | |
| US60/611,205 | 2004-09-17 | ||
| US61167604P | 2004-09-20 | 2004-09-20 | |
| US60/611,676 | 2004-09-20 | ||
| PCT/US2004/038416 WO2005050207A2 (fr) | 2003-11-14 | 2004-11-15 | Objets codes a reseaux de diffraction pour experiences multiplex |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CA2545441A1 true CA2545441A1 (fr) | 2005-06-02 |
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Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CA002545441A Abandoned CA2545441A1 (fr) | 2003-11-14 | 2004-11-15 | Objets codes a reseaux de diffraction pour experiences multiplex |
Country Status (3)
| Country | Link |
|---|---|
| EP (1) | EP1723419A2 (fr) |
| CA (1) | CA2545441A1 (fr) |
| WO (1) | WO2005050207A2 (fr) |
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| US7441703B2 (en) | 2002-08-20 | 2008-10-28 | Illumina, Inc. | Optical reader for diffraction grating-based encoded optical identification elements |
| US7872804B2 (en) | 2002-08-20 | 2011-01-18 | Illumina, Inc. | Encoded particle having a grating with variations in the refractive index |
| US7164533B2 (en) | 2003-01-22 | 2007-01-16 | Cyvera Corporation | Hybrid random bead/chip based microarray |
| US7923260B2 (en) | 2002-08-20 | 2011-04-12 | Illumina, Inc. | Method of reading encoded particles |
| US7619819B2 (en) | 2002-08-20 | 2009-11-17 | Illumina, Inc. | Method and apparatus for drug product tracking using encoded optical identification elements |
| US7901630B2 (en) | 2002-08-20 | 2011-03-08 | Illumina, Inc. | Diffraction grating-based encoded microparticle assay stick |
| US7508608B2 (en) | 2004-11-17 | 2009-03-24 | Illumina, Inc. | Lithographically fabricated holographic optical identification element |
| US7399643B2 (en) | 2002-09-12 | 2008-07-15 | Cyvera Corporation | Method and apparatus for aligning microbeads in order to interrogate the same |
| EP1540591A1 (fr) | 2002-09-12 | 2005-06-15 | Cyvera Corporation | Microparticules codees sur la base d'un reseau de diffraction pour experimentations multiplexees |
| US7092160B2 (en) | 2002-09-12 | 2006-08-15 | Illumina, Inc. | Method of manufacturing of diffraction grating-based optical identification element |
| US7433123B2 (en) | 2004-02-19 | 2008-10-07 | Illumina, Inc. | Optical identification element having non-waveguide photosensitive substrate with diffraction grating therein |
| EP2194485B1 (fr) | 2004-11-16 | 2012-10-17 | Illumina, Inc. | Procédés et appareils pour la lecture de microbilles codées |
| WO2006055735A2 (fr) | 2004-11-16 | 2006-05-26 | Illumina, Inc | Scanner dote d'un modulateur spatial de lumiere |
| US7604173B2 (en) | 2004-11-16 | 2009-10-20 | Illumina, Inc. | Holographically encoded elements for microarray and other tagging labeling applications, and method and apparatus for making and reading the same |
| US7623624B2 (en) | 2005-11-22 | 2009-11-24 | Illumina, Inc. | Method and apparatus for labeling using optical identification elements characterized by X-ray diffraction |
| US7830575B2 (en) | 2006-04-10 | 2010-11-09 | Illumina, Inc. | Optical scanner with improved scan time |
| CA2667119C (fr) * | 2006-10-18 | 2016-04-12 | Axela Inc. | Mesure de multiples analytes sur une large plage de concentrations au moyen d'une diffraction optique |
| WO2009111033A2 (fr) | 2008-03-05 | 2009-09-11 | Axela Inc. | Détection de biomarqueurs et complexes de biomarqueurs |
| CN110082501B (zh) * | 2019-04-29 | 2021-05-28 | 中南大学 | 地质岩芯空间姿态复原装置 |
Family Cites Families (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4011435A (en) * | 1974-02-01 | 1977-03-08 | Ncr Corporation | Optical indicia marking and detection system |
| US6340588B1 (en) * | 1995-04-25 | 2002-01-22 | Discovery Partners International, Inc. | Matrices with memories |
| US6096496A (en) * | 1997-06-19 | 2000-08-01 | Frankel; Robert D. | Supports incorporating vertical cavity emitting lasers and tracking apparatus for use in combinatorial synthesis |
| US20030129654A1 (en) * | 1999-04-15 | 2003-07-10 | Ilya Ravkin | Coded particles for multiplexed analysis of biological samples |
| JP4999249B2 (ja) * | 2000-03-22 | 2012-08-15 | アクセラ インコーポレーテッド | 複数アナライトのアッセイ方法およびアッセイ装置 |
| US20040126875A1 (en) * | 2002-09-12 | 2004-07-01 | Putnam Martin A. | Assay stick |
| CA2499037A1 (fr) * | 2002-09-12 | 2004-03-25 | Cyvera Corporation | Procede et appareil d'etiquetage utilisant des elements d'identification optique codes a reseau de diffraction |
-
2004
- 2004-11-15 CA CA002545441A patent/CA2545441A1/fr not_active Abandoned
- 2004-11-15 WO PCT/US2004/038416 patent/WO2005050207A2/fr not_active Ceased
- 2004-11-15 EP EP04811206A patent/EP1723419A2/fr not_active Ceased
Also Published As
| Publication number | Publication date |
|---|---|
| EP1723419A2 (fr) | 2006-11-22 |
| WO2005050207A2 (fr) | 2005-06-02 |
| WO2005050207A3 (fr) | 2005-12-29 |
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