CA2566924C - Instrument servant a surveiller l'amplification en chaine par polymerase (pcr) d'adn - Google Patents

Instrument servant a surveiller l'amplification en chaine par polymerase (pcr) d'adn Download PDF

Info

Publication number: CA2566924C
Authority: CA; Canada
Prior art keywords: instrument; emission; fresnel lens; disposed along; beam path
Prior art date: 1998-05-16
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.): Expired - Lifetime

Application number

CA2566924A

Other languages

English (en)

Other versions

CA2566924A1 (fr

Inventor

Michael R. Gambini

John G. Atwood

Eugene F. Young

Anthony L. Cerrone

Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)

Applied Biosystems LLC

Original Assignee

Applied Biosystems LLC

Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)

1998-05-16

Filing date

1999-05-17

Publication date

2011-11-29

1999-05-17 Application filed by Applied Biosystems LLC filed Critical Applied Biosystems LLC

1999-05-17 Priority claimed from CA002328609A external-priority patent/CA2328609A1/fr

1999-11-25 Publication of CA2566924A1 publication Critical patent/CA2566924A1/fr

2011-11-29 Application granted granted Critical

2011-11-29 Publication of CA2566924C publication Critical patent/CA2566924C/fr

2019-05-17 Anticipated expiration legal-status Critical

Status Expired - Lifetime legal-status Critical Current

Links

238000003752 polymerase chain reaction Methods 0.000 title claims description 33
238000012544 monitoring process Methods 0.000 title description 9
230000005284 excitation Effects 0.000 claims abstract description 126
238000006243 chemical reaction Methods 0.000 claims abstract description 51
239000004615 ingredient Substances 0.000 claims abstract description 37
230000003287 optical effect Effects 0.000 claims abstract description 32
238000000034 method Methods 0.000 claims description 35
239000003550 marker Substances 0.000 claims description 13
238000012545 processing Methods 0.000 claims description 9
230000004044 response Effects 0.000 claims description 8
108020004414 DNA Proteins 0.000 abstract description 57
102000053602 DNA Human genes 0.000 abstract description 55
239000000975 dye Substances 0.000 abstract description 43
230000010076 replication Effects 0.000 abstract description 6
239000000523 sample Substances 0.000 description 23
230000000694 effects Effects 0.000 description 10
239000000725 suspension Substances 0.000 description 10
108091008695 photoreceptors Proteins 0.000 description 7
230000004568 DNA-binding Effects 0.000 description 6
238000010606 normalization Methods 0.000 description 6
239000007850 fluorescent dye Substances 0.000 description 5
238000010438 heat treatment Methods 0.000 description 5
230000008569 process Effects 0.000 description 5
230000004543 DNA replication Effects 0.000 description 4
XAGFODPZIPBFFR-UHFFFAOYSA-N aluminium Chemical compound [Al] XAGFODPZIPBFFR-UHFFFAOYSA-N 0.000 description 4
229910052782 aluminium Inorganic materials 0.000 description 4
238000012937 correction Methods 0.000 description 4
230000001351 cycling effect Effects 0.000 description 4
238000001506 fluorescence spectroscopy Methods 0.000 description 4
230000007935 neutral effect Effects 0.000 description 4
238000004458 analytical method Methods 0.000 description 3
230000001965 increasing effect Effects 0.000 description 3
238000005259 measurement Methods 0.000 description 3
229920003023 plastic Polymers 0.000 description 3
108020001019 DNA Primers Proteins 0.000 description 2
230000004544 DNA amplification Effects 0.000 description 2
239000003155 DNA primer Substances 0.000 description 2
102000004190 Enzymes Human genes 0.000 description 2
108090000790 Enzymes Proteins 0.000 description 2
239000007900 aqueous suspension Substances 0.000 description 2
238000013480 data collection Methods 0.000 description 2
239000001046 green dye Substances 0.000 description 2
238000005286 illumination Methods 0.000 description 2
238000003384 imaging method Methods 0.000 description 2
230000010354 integration Effects 0.000 description 2
239000000463 material Substances 0.000 description 2
238000011155 quantitative monitoring Methods 0.000 description 2
230000002829 reductive effect Effects 0.000 description 2
230000000717 retained effect Effects 0.000 description 2
239000000126 substance Substances 0.000 description 2
230000007704 transition Effects 0.000 description 2
240000006829 Ficus sundaica Species 0.000 description 1
VVQNEPGJFQJSBK-UHFFFAOYSA-N Methyl methacrylate Chemical compound COC(=O)C(C)=C VVQNEPGJFQJSBK-UHFFFAOYSA-N 0.000 description 1
108091028043 Nucleic acid sequence Proteins 0.000 description 1
238000010222 PCR analysis Methods 0.000 description 1
229920005372 Plexiglas® Polymers 0.000 description 1
230000006978 adaptation Effects 0.000 description 1
230000002238 attenuated effect Effects 0.000 description 1
230000005540 biological transmission Effects 0.000 description 1
238000004061 bleaching Methods 0.000 description 1
230000008859 change Effects 0.000 description 1
230000002925 chemical effect Effects 0.000 description 1
238000009833 condensation Methods 0.000 description 1
230000005494 condensation Effects 0.000 description 1
238000011109 contamination Methods 0.000 description 1
239000002826 coolant Substances 0.000 description 1
230000004069 differentiation Effects 0.000 description 1
238000005516 engineering process Methods 0.000 description 1
230000008020 evaporation Effects 0.000 description 1
238000001704 evaporation Methods 0.000 description 1
GNBHRKFJIUUOQI-UHFFFAOYSA-N fluorescein Chemical compound O1C(=O)C2=CC=CC=C2C21C1=CC=C(O)C=C1OC1=CC(O)=CC=C21 GNBHRKFJIUUOQI-UHFFFAOYSA-N 0.000 description 1
239000003292 glue Substances 0.000 description 1
229910052736 halogen Inorganic materials 0.000 description 1
150000002367 halogens Chemical class 0.000 description 1
238000002347 injection Methods 0.000 description 1
239000007924 injection Substances 0.000 description 1
230000002452 interceptive effect Effects 0.000 description 1
239000007788 liquid Substances 0.000 description 1
238000004519 manufacturing process Methods 0.000 description 1
230000000873 masking effect Effects 0.000 description 1
238000002844 melting Methods 0.000 description 1
230000008018 melting Effects 0.000 description 1
238000012986 modification Methods 0.000 description 1
230000004048 modification Effects 0.000 description 1
239000003068 molecular probe Substances 0.000 description 1
150000007523 nucleic acids Chemical group 0.000 description 1
230000001151 other effect Effects 0.000 description 1
238000013021 overheating Methods 0.000 description 1
238000004806 packaging method and process Methods 0.000 description 1
238000012805 post-processing Methods 0.000 description 1
238000002360 preparation method Methods 0.000 description 1
230000008707 rearrangement Effects 0.000 description 1
230000003362 replicative effect Effects 0.000 description 1
PYWVYCXTNDRMGF-UHFFFAOYSA-N rhodamine B Chemical compound [Cl-].C=12C=CC(=[N+](CC)CC)C=C2OC2=CC(N(CC)CC)=CC=C2C=1C1=CC=CC=C1C(O)=O PYWVYCXTNDRMGF-UHFFFAOYSA-N 0.000 description 1
230000000630 rising effect Effects 0.000 description 1
238000012546 transfer Methods 0.000 description 1

Landscapes

Investigating, Analyzing Materials By Fluorescence Or Luminescence (AREA)
Investigating Or Analysing Materials By The Use Of Chemical Reactions (AREA)

CA2566924A 1998-05-16 1999-05-17 Instrument servant a surveiller l'amplification en chaine par polymerase (pcr) d'adn Expired - Lifetime CA2566924C (fr)

Applications Claiming Priority (5)

Application Number	Priority Date	Filing Date	Title
US8576598P	1998-05-16	1998-05-16
US60/085,765		1998-05-16
US9278498P	1998-07-14	1998-07-14
US60/092,784		1998-07-14
CA002328609A CA2328609A1 (fr)	1998-05-16	1999-05-17	Instrument servant a surveiller l'amplification en chaine par polymerase (pcr) d'adn

Related Parent Applications (1)

Application Number	Title	Priority Date	Filing Date
CA002328609A Division CA2328609A1 (fr)	1998-05-16	1999-05-17	Instrument servant a surveiller l'amplification en chaine par polymerase (pcr) d'adn

Publications (2)

Publication Number	Publication Date
CA2566924A1 CA2566924A1 (fr)	1999-11-25
CA2566924C true CA2566924C (fr)	2011-11-29

Family

ID=37682479

Family Applications (1)

Application Number	Title	Priority Date	Filing Date
CA2566924A Expired - Lifetime CA2566924C (fr)	1998-05-16	1999-05-17	Instrument servant a surveiller l'amplification en chaine par polymerase (pcr) d'adn

Country Status (1)

Country	Link
CA (1)	CA2566924C (fr)

1999
- 1999-05-17 CA CA2566924A patent/CA2566924C/fr not_active Expired - Lifetime

Also Published As

Publication number	Publication date
CA2566924A1 (fr)	1999-11-25

Legal Events

Date	Code	Title	Description
2006-11-09	EEER	Examination request
2019-07-21	MKEX	Expiry	Effective date: 20190517

Publication	Publication Date	Title
US9273353B2 (en)	2016-03-01	Instrument for monitoring polymerase chain reaction of DNA
US9671342B2 (en)	2017-06-06	Instrument for monitoring polymerase chain reaction of DNA
US8361785B2 (en)	2013-01-29	Optical instrument comprising multi-notch beam splitter
US7295316B2 (en)	2007-11-13	Fluorescent detector with automatic changing filters
CA2566924C (fr)	2011-11-29	Instrument servant a surveiller l'amplification en chaine par polymerase (pcr) d'adn
EP1619491B1 (fr)	2009-03-11	Instrument optique servant à surveiller des réactions de polymérase en chaîne d'ADN