CA2651907C - Systeme bipartite, procede et composition permettant l'expression constitutive et inductible de niveaux eleves de proteines etrangeres dans des plantes - Google Patents

Systeme bipartite, procede et composition permettant l'expression constitutive et inductible de niveaux eleves de proteines etrangeres dans des plantes Download PDF

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Publication number
CA2651907C
CA2651907C CA2651907A CA2651907A CA2651907C CA 2651907 C CA2651907 C CA 2651907C CA 2651907 A CA2651907 A CA 2651907A CA 2651907 A CA2651907 A CA 2651907A CA 2651907 C CA2651907 C CA 2651907C
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Prior art keywords
rna
virus
nucleic acid
gfp
construct
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Expired - Fee Related
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CA2651907A
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CA2651907A1 (fr
Inventor
George Lomonossoff
Li Liu
Carmen Canizares
Marc-Andre D'aoust
Louis-Philippe Vezina
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Plant Bioscience Ltd
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Plant Bioscience Ltd
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8201—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation
    • C12N15/8202—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation by biological means, e.g. cell mediated or natural vector
    • C12N15/8203—Virus mediated transformation
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8216—Methods for controlling, regulating or enhancing expression of transgenes in plant cells
    • C12N15/8218—Antisense, co-suppression, viral induced gene silencing [VIGS], post-transcriptional induced gene silencing [PTGS]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8242—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits
    • C12N15/8257—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits for the production of primary gene products, e.g. pharmaceutical products, interferon
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8242—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits
    • C12N15/8257—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits for the production of primary gene products, e.g. pharmaceutical products, interferon
    • C12N15/8258—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits for the production of primary gene products, e.g. pharmaceutical products, interferon for the production of oral vaccines (antigens) or immunoglobulins

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Molecular Biology (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Organic Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Biochemistry (AREA)
  • Plant Pathology (AREA)
  • Biophysics (AREA)
  • Microbiology (AREA)
  • Physics & Mathematics (AREA)
  • General Health & Medical Sciences (AREA)
  • Cell Biology (AREA)
  • Medicinal Chemistry (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Virology (AREA)
  • Immunology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)

Abstract

La présente invention concerne un système combiné de vecteur transgène/virus permettant l'expression de protéines hétérologues dans des plantes. Bien qu'elle soit exemplifiée relativement au phytovirus bipartite à ARN Cowpect mosaic virus (CPMV), il est possible d'utiliser avantageusement d'autres systèmes viraux bipartites selon le procédé, le système et la composition décrits en détail dans ce présent document.
CA2651907A 2006-05-22 2006-05-22 Systeme bipartite, procede et composition permettant l'expression constitutive et inductible de niveaux eleves de proteines etrangeres dans des plantes Expired - Fee Related CA2651907C (fr)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
PCT/IB2006/003548 WO2007135480A1 (fr) 2006-05-22 2006-05-22 Système bipartite, procédé et composition permettant l'expression constitutive et inductible de niveaux élevés de protéines étrangères dans des plantes

Publications (2)

Publication Number Publication Date
CA2651907A1 CA2651907A1 (fr) 2007-11-29
CA2651907C true CA2651907C (fr) 2016-12-20

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CA2651907A Expired - Fee Related CA2651907C (fr) 2006-05-22 2006-05-22 Systeme bipartite, procede et composition permettant l'expression constitutive et inductible de niveaux eleves de proteines etrangeres dans des plantes

Country Status (4)

Country Link
US (1) US8519113B2 (fr)
EP (1) EP2029755A1 (fr)
CA (1) CA2651907C (fr)
WO (1) WO2007135480A1 (fr)

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US7683238B2 (en) 2002-11-12 2010-03-23 iBio, Inc. and Fraunhofer USA, Inc. Production of pharmaceutically active proteins in sprouted seedlings
US7692063B2 (en) 2002-11-12 2010-04-06 Ibio, Inc. Production of foreign nucleic acids and polypeptides in sprout systems
ES2531125T3 (es) 2003-02-03 2015-03-10 Ibio Inc Sistema para la expresión de genes en plantas
EP1769068B1 (fr) 2004-02-20 2014-12-31 iBio, Inc. Systemes et methodes d'expression clonale dans des plantes
WO2009076778A1 (fr) 2007-11-27 2009-06-25 Medicago Inc. Particules pseudovirales (vlp) de la grippe recombinées produites dans des plantes transgéniques exprimant l'hémagglutinine
GB0800272D0 (en) * 2008-01-08 2008-02-13 Plant Bioscience Ltd Protein expression systems
SG187500A1 (en) 2008-01-21 2013-02-28 Medicago Inc Recombinant influenza virus-like particles (vlps) produced in transgenic plants expressing hemagglutinin
IN2012DN00324A (fr) 2009-06-15 2015-05-08 Plant Bioscience Ltd
HUE039100T2 (hu) 2009-06-24 2018-12-28 Medicago Inc Hemagglutinint tartalmazó kimer influenzavírus-jellegû részecskék
KR101773431B1 (ko) 2009-09-22 2017-09-12 메디카고 인코포레이티드 식물-유래 ｖｌｐ의 제조방법
US8637653B2 (en) * 2009-12-22 2014-01-28 The United States Of America, As Represented By The Secretary Of Agriculture Infectious plant viral vector and an artificial bipartite plant viral vector an infectious plant viral vector and an artificial bipartite plant viral vector
GB201002434D0 (en) 2010-02-12 2010-03-31 Plant Bioscience Ltd Non-infectious RNA source
FI20100248A0 (fi) 2010-06-11 2010-06-11 Kristiina Maekinen Menetelmä proteiinien tuottamiseksi
WO2012058762A1 (fr) 2010-11-04 2012-05-10 Medicago Inc. Système d'expression végétale
CA2824155A1 (fr) 2011-01-17 2012-07-26 Philip Morris Products S.A. Vecteurs pour l'expression d'acides amines dans des plantes
PT2665818T (pt) 2011-01-17 2017-07-05 Philip Morris Products Sa Expressão de proteínas em plantas
TWI620816B (zh) 2011-03-23 2018-04-11 苜蓿股份有限公司 植物衍生蛋白回收方法
GB201107468D0 (en) * 2011-05-04 2011-06-15 Plant Bioscience Ltd Protein expression systems
JP6011759B2 (ja) * 2011-09-16 2016-10-19 国立研究開発法人農業・食品産業技術総合研究機構 サイレンシング抑制因子およびその取得方法
NZ622731A (en) 2011-09-30 2016-04-29 Medicago Inc Increasing virus-like particle yield in plants
US11390878B2 (en) 2011-09-30 2022-07-19 Medicago Inc. Increasing protein yield in plants
ES2803508T3 (es) 2013-03-28 2021-01-27 Medicago Inc Producción de partículas similares al virus de la gripe en plantas
US12467058B2 (en) 2013-03-28 2025-11-11 Aramis Biotechnologies Inc. Influenza virus-like particle production in plants
CN103319609A (zh) * 2013-07-05 2013-09-25 刘德虎 人碱性成纤维细胞生长因子融合基因及其应用
CN103333256B (zh) * 2013-07-05 2014-11-05 中国农业科学院生物技术研究所 人酸性成纤维细胞生长因子融合蛋白及其编码基因和应用
RU2699982C2 (ru) * 2014-01-10 2019-09-11 Медикаго Инк. Энхансерные элементы cpmv
US10563213B2 (en) 2014-03-27 2020-02-18 Medicago Inc. Modified CPMV enhancer elements
RU2731295C2 (ru) 2014-07-11 2020-09-01 Медикаго Инк. Модификация продуцирования белков в растениях
AU2016208999A1 (en) 2015-01-23 2017-07-13 Medicago Inc. Rotavirus-like particle production in plants
CN110506118A (zh) * 2017-02-02 2019-11-26 杜克大学 用于控制基因表达的组合物和方法
GB201721600D0 (en) 2017-12-21 2018-02-07 Plant Bioscience Ltd Metabolic engineering
GB201808617D0 (en) 2018-05-25 2018-07-11 Plant Bioscience Ltd Scaffold modification
CN109576305B (zh) * 2018-12-14 2020-10-23 中国农业科学院郑州果树研究所 一种携带增强型绿色荧光蛋白报告基因的南瓜花叶病毒重组载体及其构建方法
WO2020181354A1 (fr) * 2019-03-14 2020-09-17 Mitsubishi Tanabe Pharma Corporation Activateur d'expression de plante endogène
GB201908431D0 (en) 2019-06-12 2019-07-24 Plant Bioscience Ltd Biosynthetic genes and polypeptides
GB201909104D0 (en) 2019-06-25 2019-08-07 Plant Bioscience Ltd Transferase enzymes
CN111979262A (zh) * 2020-06-08 2020-11-24 杭州师范大学 植物改造
WO2022260849A1 (fr) 2021-06-09 2022-12-15 Nant Holdings Ip, Llc Procédés et systèmes de production d'une protéine d'intérêt dans une plante
GB202209501D0 (en) 2022-06-29 2022-08-10 Plant Bioscience Ltd Biosynthetic enzymes
KR20250082120A (ko) * 2023-11-29 2025-06-09 서강대학교산학협력단 검은눈콩 모자이크 바이러스의 바이러스 유사 입자의 제조방법 및 이의 활용

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Also Published As

Publication number Publication date
US8519113B2 (en) 2013-08-27
CA2651907A1 (fr) 2007-11-29
US20090181460A1 (en) 2009-07-16
EP2029755A1 (fr) 2009-03-04
WO2007135480A1 (fr) 2007-11-29

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