CA2658926C - Vecteur cosmide servant a transformer un vegetal et son utilisation - Google Patents

Vecteur cosmide servant a transformer un vegetal et son utilisation Download PDF

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Publication number
CA2658926C
CA2658926C CA2658926A CA2658926A CA2658926C CA 2658926 C CA2658926 C CA 2658926C CA 2658926 A CA2658926 A CA 2658926A CA 2658926 A CA2658926 A CA 2658926A CA 2658926 C CA2658926 C CA 2658926C
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Canada
Prior art keywords
plasmid
vector
nucleotide sequence
gene
seq
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CA2658926A
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CA2658926A1 (fr
Inventor
Yoshimitsu Takakura
Toshihiko Komari
Yuji Ishida
Toshiyuki Komori
Yukoh Hiei
Toshiki Mine
Teruyuki Imayama
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Kaneka Corp
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Japan Tobacco Inc
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8201—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation
    • C12N15/8202—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation by biological means, e.g. cell mediated or natural vector
    • C12N15/8205—Agrobacterium mediated transformation

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Organic Chemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • Plant Pathology (AREA)
  • Cell Biology (AREA)
  • Biophysics (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Physics & Mathematics (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)

Abstract

La présente invention concerne un vecteur innovant pour la transformation de végétaux. Le vecteur est un cosmide qui a une longueur totale inférieure ou égale à 15 kb et qui possède les propriétés suivantes : (1) le vecteur contient une origine de réplication du plasmide IncP mais il ne contient pas d'origine de réplication d'autres plasmides ; (2) le vecteur contient le gène trfA1 du plasmide IncP ; (3) le vecteur contient oriT du plasmide IncP ; (4) le vecteur contient le gène incC1 du plasmide IncP ; (5) le vecteur contient un site cos d'un phage lambda et le site cos se trouve à l'extérieur de l'ADN-T ; (6) le vecteur contient un gène de résistance à un médicament qui peut être exprimé chez Escherichia coli et chez une bactérie appartenant au genre Agrobacterium ; (7) le vecteur contient une séquence de bordure droite de l'ADN-T d'une bactérie appartenant au genre Agrobacterium ; (8) le vecteur contient une séquence de bordure gauche de l'ADN-T d'une bactérie appartenant au genre Agrobacterium ; (9) le vecteur contient un gène marqueur sélectif de la transformation des végétaux, le gène marqueur sélectif se trouvant entre les séquences mentionnées en (7) et (8) et pouvant être exprimé dans un végétal ; et (10) le vecteur contient un site de reconnaissance d'enzyme de restriction pour le clonage d'un gène étranger, le site de reconnaissance d'enzyme de restriction se trouvant entre les séquences mentionnées en (7) et (8).
CA2658926A 2006-06-23 2007-06-25 Vecteur cosmide servant a transformer un vegetal et son utilisation Active CA2658926C (fr)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
JPPCT/JP2006/312633 2006-06-23
PCT/JP2006/312633 WO2008001414A1 (fr) 2006-06-23 2006-06-23 Vecteur cosmide destiné à transformer une plante et son procédé d'utilisation
PCT/JP2007/062720 WO2007148819A1 (fr) 2006-06-23 2007-06-25 Cosmide vecteur pour la transformation de végétaux et son utilisation

Publications (2)

Publication Number Publication Date
CA2658926A1 CA2658926A1 (fr) 2007-12-27
CA2658926C true CA2658926C (fr) 2016-01-12

Family

ID=38833552

Family Applications (1)

Application Number Title Priority Date Filing Date
CA2658926A Active CA2658926C (fr) 2006-06-23 2007-06-25 Vecteur cosmide servant a transformer un vegetal et son utilisation

Country Status (6)

Country Link
US (2) US8298819B2 (fr)
EP (1) EP2042602B1 (fr)
CN (1) CN101484580B (fr)
AU (1) AU2007261964B2 (fr)
CA (1) CA2658926C (fr)
WO (2) WO2008001414A1 (fr)

Families Citing this family (14)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US9617551B2 (en) * 2010-07-29 2017-04-11 Dow Agrosciences Llc Method of increasing plant transformation frequency using modified strains of Agrobacteria
JP2016111926A (ja) 2013-03-29 2016-06-23 日本たばこ産業株式会社 植物の形質転換方法に使用するためのアグロバクテリウム細菌
CA2931140C (fr) * 2014-02-05 2022-08-30 Fraunhofer-Gesellschaft Zur Forderung Der Angewandten Forschung E.V. Sequencage sans erreur d'adn
WO2015118640A1 (fr) 2014-02-06 2015-08-13 日本たばこ産業株式会社 Procédé d'acquisition de cellule transformée végétale
CN106793760B (zh) 2014-06-27 2021-03-09 日本烟草产业株式会社 病毒抗性烟草及其制备方法
JP2018027020A (ja) * 2014-12-24 2018-02-22 日本たばこ産業株式会社 アグロバクテリウム菌を用いた、ソルガム属植物への遺伝子導入方法およびソルガム属植物の形質転換植物の作成方法
GB201514510D0 (en) * 2015-08-14 2015-09-30 Nemesis Bioscience Ltd Delivery vehicle
DK3341483T3 (da) 2015-08-28 2020-03-16 Pioneer Hi Bred Int Ochrobactrum-medieret transformation af planter
WO2018038249A1 (fr) 2016-08-26 2018-03-01 日本たばこ産業株式会社 Tabac résistant aux virus et son procédé de sélection
CN108997484B (zh) 2017-06-07 2020-07-24 中国农业科学院作物科学研究所 小麦TaWox5基因在提高小麦转化效率中的应用
EP3833764A4 (fr) * 2018-08-07 2021-10-13 CJ Cheiljedang Corporation Molécules d'acide nucléique comprenant un brin codant pour le variant inc
CN108866231B (zh) * 2018-08-15 2021-06-25 江西省农业科学院水稻研究所 东野型水稻胞质雄性不育恢复基因Rf(DW)8的检测引物、试剂盒及应用与检测方法
US20240052358A1 (en) 2020-12-28 2024-02-15 Kaneka Corporation Method of producing transformed cell or plant body of maize, nucleic acid construct, and method of introducing nucleic acid into cell or plant body of maize
CN117987436B (zh) * 2024-04-03 2024-06-25 南京鸿明生物科技有限公司 一种双链靶dna序列的制备方法

Family Cites Families (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5733744A (en) * 1995-01-13 1998-03-31 Cornell Research Foundation, Inc. Binary BAC vector
AU694393B2 (en) 1996-09-26 1998-07-16 National Institute Of Agrobiological Resources, Ministry Of Agriculture, Forestry And Fisheries New high capacity binary shuttle vector
JP3350753B2 (ja) 1996-09-26 2002-11-25 科学技術振興事業団 新規大容量バイナリーシャトルベクター
CA2301707A1 (fr) * 1997-08-25 1999-03-04 Nunhems Zaden B.V. Transformation de plantes provoquee par une agrobacterie amelioree
US20030188344A1 (en) * 2000-12-12 2003-10-02 Lynn David G. Compositions and methods for agrobacterium transformation of plants
JP4068377B2 (ja) 2002-03-29 2008-03-26 株式会社ニデック 真空蒸着装置
US7812222B2 (en) 2003-08-13 2010-10-12 Japan Tobacco Inc. Method of transducing gene into plant material
WO2005040374A1 (fr) 2003-10-24 2005-05-06 Japan Tobacco Inc. Procede de selection de fragment d'adn genomique

Also Published As

Publication number Publication date
AU2007261964A1 (en) 2007-12-27
EP2042602A4 (fr) 2010-04-21
US8298819B2 (en) 2012-10-30
EP2042602B1 (fr) 2012-12-26
EP2042602A1 (fr) 2009-04-01
CN101484580B (zh) 2012-01-25
CN101484580A (zh) 2009-07-15
US20100132068A1 (en) 2010-05-27
US20130091599A1 (en) 2013-04-11
CA2658926A1 (fr) 2007-12-27
WO2007148819A1 (fr) 2007-12-27
AU2007261964B2 (en) 2014-10-02
WO2008001414A1 (fr) 2008-01-03
WO2007148819A9 (fr) 2008-12-11

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