CA2673577A1 - Detection de procaryotes induisant une corrosion - Google Patents

Detection de procaryotes induisant une corrosion Download PDF

Info

Publication number: CA2673577A1
Authority: CA; Canada
Prior art keywords: sample; dna; seq; nucleic acid; industrial water
Prior art date: 2006-10-17
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.): Abandoned

Application number

CA002673577A

Other languages

English (en)

Inventor

Vanessa Madrid

Andrei Chistoserdov

Michael Chapman

Brian Price

Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)

Individual

Original Assignee

University of Louisiana at Lafayette

Multi Chem Group LLC

Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)

2006-10-17

Filing date

2007-10-17

Publication date

2008-06-05

2007-10-17 Application filed by University of Louisiana at Lafayette, Multi Chem Group LLC filed Critical University of Louisiana at Lafayette

2008-06-05 Publication of CA2673577A1 publication Critical patent/CA2673577A1/fr

Status Abandoned legal-status Critical Current

Links

241000894006 Bacteria Species 0.000 title claims abstract description 83
238000001514 detection method Methods 0.000 title claims abstract description 38
238000005260 corrosion Methods 0.000 title abstract description 30
230000007797 corrosion Effects 0.000 title abstract description 30
230000001939 inductive effect Effects 0.000 title description 2
238000000034 method Methods 0.000 claims abstract description 161
238000003752 polymerase chain reaction Methods 0.000 claims abstract description 74
QAOWNCQODCNURD-UHFFFAOYSA-L Sulfate Chemical compound [O-]S([O-])(=O)=O QAOWNCQODCNURD-UHFFFAOYSA-L 0.000 claims abstract description 58
150000007523 nucleic acids Chemical class 0.000 claims abstract description 43
102000039446 nucleic acids Human genes 0.000 claims abstract description 34
108020004707 nucleic acids Proteins 0.000 claims abstract description 34
108010028074 Hydrogensulfite Reductase Proteins 0.000 claims abstract description 10
239000000523 sample Substances 0.000 claims description 141
108020004414 DNA Proteins 0.000 claims description 117
239000000872 buffer Substances 0.000 claims description 51
238000001914 filtration Methods 0.000 claims description 35
239000000203 mixture Substances 0.000 claims description 27
230000003321 amplification Effects 0.000 claims description 21
239000008235 industrial water Substances 0.000 claims description 21
238000003199 nucleic acid amplification method Methods 0.000 claims description 21
230000027455 binding Effects 0.000 claims description 18
238000012408 PCR amplification Methods 0.000 claims description 15
108091028043 Nucleic acid sequence Proteins 0.000 claims description 10
239000002033 PVDF binder Substances 0.000 claims description 5
229920002981 polyvinylidene fluoride Polymers 0.000 claims description 5
108090000623 proteins and genes Proteins 0.000 abstract description 81
238000003753 real-time PCR Methods 0.000 abstract description 65
239000013049 sediment Substances 0.000 abstract description 65
244000005700 microbiome Species 0.000 abstract description 34
230000000813 microbial effect Effects 0.000 abstract description 23
230000001580 bacterial effect Effects 0.000 abstract description 20
238000000605 extraction Methods 0.000 abstract description 18
230000009467 reduction Effects 0.000 abstract description 15
102000004190 Enzymes Human genes 0.000 abstract description 9
108090000790 Enzymes Proteins 0.000 abstract description 9
238000011534 incubation Methods 0.000 abstract description 9
238000012360 testing method Methods 0.000 abstract description 6
238000012545 processing Methods 0.000 abstract description 5
239000012530 fluid Substances 0.000 abstract description 4
230000037361 pathway Effects 0.000 abstract description 4
241000203069 Archaea Species 0.000 abstract description 2
230000001419 dependent effect Effects 0.000 abstract 1
XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 50
239000012528 membrane Substances 0.000 description 45
238000006243 chemical reaction Methods 0.000 description 40
238000011002 quantification Methods 0.000 description 31
238000003556 assay Methods 0.000 description 30
210000004027 cell Anatomy 0.000 description 23
239000000047 product Substances 0.000 description 23
230000007613 environmental effect Effects 0.000 description 22
101150095420 dsr gene Proteins 0.000 description 21
108091093088 Amplicon Proteins 0.000 description 20
238000007400 DNA extraction Methods 0.000 description 20
239000013612 plasmid Substances 0.000 description 20
238000007399 DNA isolation Methods 0.000 description 19
239000007788 liquid Substances 0.000 description 19
241000218691 Cupressaceae Species 0.000 description 17
241000605762 Desulfovibrio vulgaris Species 0.000 description 16
239000011324 bead Substances 0.000 description 15
108010067770 Endopeptidase K Proteins 0.000 description 12
230000015572 biosynthetic process Effects 0.000 description 12
230000005764 inhibitory process Effects 0.000 description 12
241000186539 Desulfotomaculum ruminis Species 0.000 description 11
238000012544 monitoring process Methods 0.000 description 11
239000000243 solution Substances 0.000 description 11
102000053602 DNA Human genes 0.000 description 10
239000004599 antimicrobial Substances 0.000 description 10
230000002860 competitive effect Effects 0.000 description 10
108090000854 Oxidoreductases Proteins 0.000 description 9
238000004458 analytical method Methods 0.000 description 9
239000002689 soil Substances 0.000 description 9
102000004316 Oxidoreductases Human genes 0.000 description 8
239000000539 dimer Substances 0.000 description 8
230000003287 optical effect Effects 0.000 description 8
238000010791 quenching Methods 0.000 description 8
230000000171 quenching effect Effects 0.000 description 8
239000000975 dye Substances 0.000 description 7
239000007850 fluorescent dye Substances 0.000 description 7
239000003068 molecular probe Substances 0.000 description 7
238000002360 preparation method Methods 0.000 description 7
238000012546 transfer Methods 0.000 description 7
ZYFVNVRFVHJEIU-UHFFFAOYSA-N PicoGreen Chemical compound CN(C)CCCN(CCCN(C)C)C1=CC(=CC2=[N+](C3=CC=CC=C3S2)C)C2=CC=CC=C2N1C1=CC=CC=C1 ZYFVNVRFVHJEIU-UHFFFAOYSA-N 0.000 description 6
238000005119 centrifugation Methods 0.000 description 6
239000003153 chemical reaction reagent Substances 0.000 description 6
238000010790 dilution Methods 0.000 description 6
239000012895 dilution Substances 0.000 description 6
230000000694 effects Effects 0.000 description 6
238000001506 fluorescence spectroscopy Methods 0.000 description 6
238000009396 hybridization Methods 0.000 description 6
238000004519 manufacturing process Methods 0.000 description 6
230000008018 melting Effects 0.000 description 6
238000002844 melting Methods 0.000 description 6
239000002245 particle Substances 0.000 description 6
238000005070 sampling Methods 0.000 description 6
108091032973 (ribonucleotides)n+m Proteins 0.000 description 5
108010032655 Adenylyl-sulfate reductase Proteins 0.000 description 5
KCXVZYZYPLLWCC-UHFFFAOYSA-N EDTA Chemical compound OC(=O)CN(CC(O)=O)CCN(CC(O)=O)CC(O)=O KCXVZYZYPLLWCC-UHFFFAOYSA-N 0.000 description 5
238000000137 annealing Methods 0.000 description 5
238000013461 design Methods 0.000 description 5
239000011521 glass Substances 0.000 description 5
239000003112 inhibitor Substances 0.000 description 5
239000003550 marker Substances 0.000 description 5
230000002503 metabolic effect Effects 0.000 description 5
239000013642 negative control Substances 0.000 description 5
238000004321 preservation Methods 0.000 description 5
238000013207 serial dilution Methods 0.000 description 5
238000003860 storage Methods 0.000 description 5
229910021653 sulphate ion Inorganic materials 0.000 description 5
239000002028 Biomass Substances 0.000 description 4
LZZYPRNAOMGNLH-UHFFFAOYSA-M Cetrimonium bromide Chemical compound [Br-].CCCCCCCCCCCCCCCC[N+](C)(C)C LZZYPRNAOMGNLH-UHFFFAOYSA-M 0.000 description 4
238000007900 DNA-DNA hybridization Methods 0.000 description 4
KFZMGEQAYNKOFK-UHFFFAOYSA-N Isopropanol Chemical compound CC(C)O KFZMGEQAYNKOFK-UHFFFAOYSA-N 0.000 description 4
108091034117 Oligonucleotide Proteins 0.000 description 4
238000002944 PCR assay Methods 0.000 description 4
238000011529 RT qPCR Methods 0.000 description 4
FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 description 4
LSNNMFCWUKXFEE-UHFFFAOYSA-N Sulfurous acid Chemical compound OS(O)=O LSNNMFCWUKXFEE-UHFFFAOYSA-N 0.000 description 4
238000000246 agarose gel electrophoresis Methods 0.000 description 4
238000011088 calibration curve Methods 0.000 description 4
230000015556 catabolic process Effects 0.000 description 4
239000006285 cell suspension Substances 0.000 description 4
238000012512 characterization method Methods 0.000 description 4
230000000875 corresponding effect Effects 0.000 description 4
238000006731 degradation reaction Methods 0.000 description 4
239000007789 gas Substances 0.000 description 4
230000014509 gene expression Effects 0.000 description 4
230000001965 increasing effect Effects 0.000 description 4
239000000463 material Substances 0.000 description 4
238000005259 measurement Methods 0.000 description 4
VNWKTOKETHGBQD-UHFFFAOYSA-N methane Chemical compound C VNWKTOKETHGBQD-UHFFFAOYSA-N 0.000 description 4
239000013641 positive control Substances 0.000 description 4
102000004169 proteins and genes Human genes 0.000 description 4
238000003757 reverse transcription PCR Methods 0.000 description 4
230000035945 sensitivity Effects 0.000 description 4
239000001488 sodium phosphate Substances 0.000 description 4
229910000162 sodium phosphate Inorganic materials 0.000 description 4
238000012421 spiking Methods 0.000 description 4
RYFMWSXOAZQYPI-UHFFFAOYSA-K trisodium phosphate Chemical compound [Na+].[Na+].[Na+].[O-]P([O-])([O-])=O RYFMWSXOAZQYPI-UHFFFAOYSA-K 0.000 description 4
239000003643 water by type Substances 0.000 description 4
241000588724 Escherichia coli Species 0.000 description 3
230000010757 Reduction Activity Effects 0.000 description 3
108010006785 Taq Polymerase Proteins 0.000 description 3
JLCPHMBAVCMARE-UHFFFAOYSA-N [3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-[[3-[[3-[[3-[[3-[[3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-hydroxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methyl [5-(6-aminopurin-9-yl)-2-(hydroxymethyl)oxolan-3-yl] hydrogen phosphate Polymers Cc1cn(C2CC(OP(O)(=O)OCC3OC(CC3OP(O)(=O)OCC3OC(CC3O)n3cnc4c3nc(N)[nH]c4=O)n3cnc4c3nc(N)[nH]c4=O)C(COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3CO)n3cnc4c(N)ncnc34)n3ccc(N)nc3=O)n3cnc4c(N)ncnc34)n3ccc(N)nc3=O)n3ccc(N)nc3=O)n3ccc(N)nc3=O)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cc(C)c(=O)[nH]c3=O)n3cc(C)c(=O)[nH]c3=O)n3ccc(N)nc3=O)n3cc(C)c(=O)[nH]c3=O)n3cnc4c3nc(N)[nH]c4=O)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)O2)c(=O)[nH]c1=O JLCPHMBAVCMARE-UHFFFAOYSA-N 0.000 description 3
QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 description 3
210000000349 chromosome Anatomy 0.000 description 3
230000003247 decreasing effect Effects 0.000 description 3
239000012153 distilled water Substances 0.000 description 3
230000009977 dual effect Effects 0.000 description 3
238000002474 experimental method Methods 0.000 description 3
239000011536 extraction buffer Substances 0.000 description 3
239000012634 fragment Substances 0.000 description 3
239000013505 freshwater Substances 0.000 description 3
239000000499 gel Substances 0.000 description 3
230000002068 genetic effect Effects 0.000 description 3
239000004021 humic acid Substances 0.000 description 3
XEEYBQQBJWHFJM-UHFFFAOYSA-N iron Substances [Fe] XEEYBQQBJWHFJM-UHFFFAOYSA-N 0.000 description 3
229910052751 metal Inorganic materials 0.000 description 3
239000002184 metal Substances 0.000 description 3
229910052760 oxygen Inorganic materials 0.000 description 3
239000001301 oxygen Substances 0.000 description 3
238000000746 purification Methods 0.000 description 3
238000011160 research Methods 0.000 description 3
UEUXEKPTXMALOB-UHFFFAOYSA-J tetrasodium;2-[2-[bis(carboxylatomethyl)amino]ethyl-(carboxylatomethyl)amino]acetate Chemical compound [Na+].[Na+].[Na+].[Na+].[O-]C(=O)CN(CC([O-])=O)CCN(CC([O-])=O)CC([O-])=O UEUXEKPTXMALOB-UHFFFAOYSA-J 0.000 description 3
230000000007 visual effect Effects 0.000 description 3
238000013382 DNA quantification Methods 0.000 description 2
108010014303 DNA-directed DNA polymerase Proteins 0.000 description 2
102000016928 DNA-directed DNA polymerase Human genes 0.000 description 2
102000004533 Endonucleases Human genes 0.000 description 2
108010042407 Endonucleases Proteins 0.000 description 2
VTLYFUHAOXGGBS-UHFFFAOYSA-N Fe3+ Chemical compound [Fe+3] VTLYFUHAOXGGBS-UHFFFAOYSA-N 0.000 description 2
108010010803 Gelatin Proteins 0.000 description 2
102000004020 Oxygenases Human genes 0.000 description 2
108090000417 Oxygenases Proteins 0.000 description 2
108091005804 Peptidases Proteins 0.000 description 2
102000035195 Peptidases Human genes 0.000 description 2
238000009825 accumulation Methods 0.000 description 2
239000002253 acid Substances 0.000 description 2
125000003275 alpha amino acid group Chemical group 0.000 description 2
238000013459 approach Methods 0.000 description 2
230000008901 benefit Effects 0.000 description 2
230000003115 biocidal effect Effects 0.000 description 2
239000003139 biocide Substances 0.000 description 2
230000005587 bubbling Effects 0.000 description 2
238000010367 cloning Methods 0.000 description 2
239000002299 complementary DNA Substances 0.000 description 2
230000002596 correlated effect Effects 0.000 description 2
238000013211 curve analysis Methods 0.000 description 2
SUYVUBYJARFZHO-RRKCRQDMSA-N dATP Chemical compound C1=NC=2C(N)=NC=NC=2N1[C@H]1C[C@H](O)[C@@H](COP(O)(=O)OP(O)(=O)OP(O)(O)=O)O1 SUYVUBYJARFZHO-RRKCRQDMSA-N 0.000 description 2
SUYVUBYJARFZHO-UHFFFAOYSA-N dATP Natural products C1=NC=2C(N)=NC=NC=2N1C1CC(O)C(COP(O)(=O)OP(O)(=O)OP(O)(O)=O)O1 SUYVUBYJARFZHO-UHFFFAOYSA-N 0.000 description 2
RGWHQCVHVJXOKC-SHYZEUOFSA-J dCTP(4-) Chemical compound O=C1N=C(N)C=CN1[C@@H]1O[C@H](COP([O-])(=O)OP([O-])(=O)OP([O-])([O-])=O)[C@@H](O)C1 RGWHQCVHVJXOKC-SHYZEUOFSA-J 0.000 description 2
HAAZLUGHYHWQIW-KVQBGUIXSA-N dGTP Chemical compound C1=NC=2C(=O)NC(N)=NC=2N1[C@H]1C[C@H](O)[C@@H](COP(O)(=O)OP(O)(=O)OP(O)(O)=O)O1 HAAZLUGHYHWQIW-KVQBGUIXSA-N 0.000 description 2
NHVNXKFIZYSCEB-XLPZGREQSA-N dTTP Chemical compound O=C1NC(=O)C(C)=CN1[C@@H]1O[C@H](COP(O)(=O)OP(O)(=O)OP(O)(O)=O)[C@@H](O)C1 NHVNXKFIZYSCEB-XLPZGREQSA-N 0.000 description 2
238000011161 development Methods 0.000 description 2
238000010494 dissociation reaction Methods 0.000 description 2
230000005593 dissociations Effects 0.000 description 2
238000005553 drilling Methods 0.000 description 2
238000005516 engineering process Methods 0.000 description 2
239000002509 fulvic acid Substances 0.000 description 2
239000008273 gelatin Substances 0.000 description 2
229920000159 gelatin Polymers 0.000 description 2
235000019322 gelatine Nutrition 0.000 description 2
235000011852 gelatine desserts Nutrition 0.000 description 2
239000003673 groundwater Substances 0.000 description 2
238000010438 heat treatment Methods 0.000 description 2
239000012535 impurity Substances 0.000 description 2
229910052742 iron Inorganic materials 0.000 description 2
-1 iron ions Chemical class 0.000 description 2
238000002955 isolation Methods 0.000 description 2
238000009630 liquid culture Methods 0.000 description 2
239000008239 natural water Substances 0.000 description 2
238000005375 photometry Methods 0.000 description 2
238000006116 polymerization reaction Methods 0.000 description 2
235000019833 protease Nutrition 0.000 description 2
238000004445 quantitative analysis Methods 0.000 description 2
239000002901 radioactive waste Substances 0.000 description 2
238000011084 recovery Methods 0.000 description 2
239000011780 sodium chloride Substances 0.000 description 2
241000894007 species Species 0.000 description 2
238000010186 staining Methods 0.000 description 2
238000004174 sulfur cycle Methods 0.000 description 2
238000003786 synthesis reaction Methods 0.000 description 2
230000002277 temperature effect Effects 0.000 description 2
238000011179 visual inspection Methods 0.000 description 2
108020004465 16S ribosomal RNA Proteins 0.000 description 1
QKNYBSVHEMOAJP-UHFFFAOYSA-N 2-amino-2-(hydroxymethyl)propane-1,3-diol;hydron;chloride Chemical compound Cl.OCC(N)(CO)CO QKNYBSVHEMOAJP-UHFFFAOYSA-N 0.000 description 1
FWBHETKCLVMNFS-UHFFFAOYSA-N 4',6-Diamino-2-phenylindol Chemical compound C1=CC(C(=N)N)=CC=C1C1=CC2=CC=C(C(N)=N)C=C2N1 FWBHETKCLVMNFS-UHFFFAOYSA-N 0.000 description 1
CYDQOEWLBCCFJZ-UHFFFAOYSA-N 4-(4-fluorophenyl)oxane-4-carboxylic acid Chemical compound C=1C=C(F)C=CC=1C1(C(=O)O)CCOCC1 CYDQOEWLBCCFJZ-UHFFFAOYSA-N 0.000 description 1
241000588813 Alcaligenes faecalis Species 0.000 description 1
NLXLAEXVIDQMFP-UHFFFAOYSA-N Ammonia chloride Chemical compound [NH4+].[Cl-] NLXLAEXVIDQMFP-UHFFFAOYSA-N 0.000 description 1
108020000946 Bacterial DNA Proteins 0.000 description 1
101000623895 Bos taurus Mucin-15 Proteins 0.000 description 1
101100278586 Cereibacter sphaeroides dmsA gene Proteins 0.000 description 1
UDMBCSSLTHHNCD-UHFFFAOYSA-N Coenzym Q(11) Natural products C1=NC=2C(N)=NC=NC=2N1C1OC(COP(O)(O)=O)C(O)C1O UDMBCSSLTHHNCD-UHFFFAOYSA-N 0.000 description 1
ZAQJHHRNXZUBTE-NQXXGFSBSA-N D-ribulose Chemical compound OC[C@@H](O)[C@@H](O)C(=O)CO ZAQJHHRNXZUBTE-NQXXGFSBSA-N 0.000 description 1
YAHZABJORDUQGO-NQXXGFSBSA-N D-ribulose 1,5-bisphosphate Chemical compound OP(=O)(O)OC[C@@H](O)[C@@H](O)C(=O)COP(O)(O)=O YAHZABJORDUQGO-NQXXGFSBSA-N 0.000 description 1
ZAQJHHRNXZUBTE-UHFFFAOYSA-N D-threo-2-Pentulose Natural products OCC(O)C(O)C(=O)CO ZAQJHHRNXZUBTE-UHFFFAOYSA-N 0.000 description 1
230000004568 DNA-binding Effects 0.000 description 1
241000205142 Desulfobacterium autotrophicum Species 0.000 description 1
241000605716 Desulfovibrio Species 0.000 description 1
101100278590 Desulfovibrio vulgaris (strain ATCC 29579 / DSM 644 / NCIMB 8303 / VKM B-1760 / Hildenborough) dsvA gene Proteins 0.000 description 1
101100278592 Desulfovibrio vulgaris (strain ATCC 29579 / DSM 644 / NCIMB 8303 / VKM B-1760 / Hildenborough) dsvB gene Proteins 0.000 description 1
DGAQECJNVWCQMB-PUAWFVPOSA-M Ilexoside XXIX Chemical compound C[C@@H]1CC[C@@]2(CC[C@@]3(C(=CC[C@H]4[C@]3(CC[C@@H]5[C@@]4(CC[C@@H](C5(C)C)OS(=O)(=O)[O-])C)C)[C@@H]2[C@]1(C)O)C)C(=O)O[C@H]6[C@@H]([C@H]([C@@H]([C@H](O6)CO)O)O)O.[Na+] DGAQECJNVWCQMB-PUAWFVPOSA-M 0.000 description 1
CSNNHWWHGAXBCP-UHFFFAOYSA-L Magnesium sulfate Chemical class [Mg+2].[O-][S+2]([O-])([O-])[O-] CSNNHWWHGAXBCP-UHFFFAOYSA-L 0.000 description 1
229920002274 Nalgene Polymers 0.000 description 1
238000009004 PCR Kit Methods 0.000 description 1
238000002123 RNA extraction Methods 0.000 description 1
241000863430 Shewanella Species 0.000 description 1
241000237854 Sipunculus nudus Species 0.000 description 1
101800002927 Small subunit Proteins 0.000 description 1
NINIDFKCEFEMDL-UHFFFAOYSA-N Sulfur Chemical compound [S] NINIDFKCEFEMDL-UHFFFAOYSA-N 0.000 description 1
241001533234 Sulfurimonas denitrificans Species 0.000 description 1
UCKMPCXJQFINFW-UHFFFAOYSA-N Sulphide Chemical compound [S-2] UCKMPCXJQFINFW-UHFFFAOYSA-N 0.000 description 1
QAOWNCQODCNURD-KHWXYDKHSA-N ac1l2g1h Chemical compound O[35S](O)(=O)=O QAOWNCQODCNURD-KHWXYDKHSA-N 0.000 description 1
DPKHZNPWBDQZCN-UHFFFAOYSA-N acridine orange free base Chemical compound C1=CC(N(C)C)=CC2=NC3=CC(N(C)C)=CC=C3C=C21 DPKHZNPWBDQZCN-UHFFFAOYSA-N 0.000 description 1
UDMBCSSLTHHNCD-KQYNXXCUSA-N adenosine 5'-monophosphate Chemical compound C1=NC=2C(N)=NC=NC=2N1[C@@H]1O[C@H](COP(O)(O)=O)[C@@H](O)[C@H]1O UDMBCSSLTHHNCD-KQYNXXCUSA-N 0.000 description 1
229960003190 adenosine monophosphate Drugs 0.000 description 1
LNQVTSROQXJCDD-UHFFFAOYSA-N adenosine monophosphate Natural products C1=NC=2C(N)=NC=NC=2N1C1OC(CO)C(OP(O)(O)=O)C1O LNQVTSROQXJCDD-UHFFFAOYSA-N 0.000 description 1
239000011543 agarose gel Substances 0.000 description 1
229940005347 alcaligenes faecalis Drugs 0.000 description 1
235000019270 ammonium chloride Nutrition 0.000 description 1
239000007900 aqueous suspension Substances 0.000 description 1
DZBUGLKDJFMEHC-UHFFFAOYSA-N benzoquinolinylidene Natural products C1=CC=CC2=CC3=CC=CC=C3N=C21 DZBUGLKDJFMEHC-UHFFFAOYSA-N 0.000 description 1
238000010876 biochemical test Methods 0.000 description 1
229940041514 candida albicans extract Drugs 0.000 description 1
210000002421 cell wall Anatomy 0.000 description 1
239000003638 chemical reducing agent Substances 0.000 description 1
239000013611 chromosomal DNA Substances 0.000 description 1
238000000658 coextraction Methods 0.000 description 1
238000010835 comparative analysis Methods 0.000 description 1
230000000295 complement effect Effects 0.000 description 1
150000001875 compounds Chemical class 0.000 description 1
239000000356 contaminant Substances 0.000 description 1
238000011109 contamination Methods 0.000 description 1
230000001627 detrimental effect Effects 0.000 description 1
229940079920 digestives acid preparations Drugs 0.000 description 1
ZPWVASYFFYYZEW-UHFFFAOYSA-L dipotassium hydrogen phosphate Chemical compound [K+].[K+].OP([O-])([O-])=O ZPWVASYFFYYZEW-UHFFFAOYSA-L 0.000 description 1
229910000396 dipotassium phosphate Inorganic materials 0.000 description 1
235000019797 dipotassium phosphate Nutrition 0.000 description 1
239000006185 dispersion Substances 0.000 description 1
238000009826 distribution Methods 0.000 description 1
101150052245 dsrA gene Proteins 0.000 description 1
101150116515 dsrB gene Proteins 0.000 description 1
239000002532 enzyme inhibitor Substances 0.000 description 1
230000002349 favourable effect Effects 0.000 description 1
238000000855 fermentation Methods 0.000 description 1
230000004151 fermentation Effects 0.000 description 1
IMBKASBLAKCLEM-UHFFFAOYSA-L ferrous ammonium sulfate (anhydrous) Chemical compound [NH4+].[NH4+].[Fe+2].[O-]S([O-])(=O)=O.[O-]S([O-])(=O)=O IMBKASBLAKCLEM-UHFFFAOYSA-L 0.000 description 1
238000001502 gel electrophoresis Methods 0.000 description 1
230000036541 health Effects 0.000 description 1
238000000703 high-speed centrifugation Methods 0.000 description 1
229930195733 hydrocarbon Natural products 0.000 description 1
150000002430 hydrocarbons Chemical class 0.000 description 1
238000011081 inoculation Methods 0.000 description 1
230000004807 localization Effects 0.000 description 1
239000011159 matrix material Substances 0.000 description 1
230000001404 mediated effect Effects 0.000 description 1
239000000155 melt Substances 0.000 description 1
230000037353 metabolic pathway Effects 0.000 description 1
238000007479 molecular analysis Methods 0.000 description 1
238000010369 molecular cloning Methods 0.000 description 1
239000003147 molecular marker Substances 0.000 description 1
150000002825 nitriles Chemical class 0.000 description 1
238000010899 nucleation Methods 0.000 description 1
239000003129 oil well Substances 0.000 description 1
239000005416 organic matter Substances 0.000 description 1
239000008188 pellet Substances 0.000 description 1
238000013081 phylogenetic analysis Methods 0.000 description 1
230000035479 physiological effects, processes and functions Effects 0.000 description 1
239000004033 plastic Substances 0.000 description 1
230000008569 process Effects 0.000 description 1
230000002285 radioactive effect Effects 0.000 description 1
230000029058 respiratory gaseous exchange Effects 0.000 description 1
238000000926 separation method Methods 0.000 description 1
238000012163 sequencing technique Methods 0.000 description 1
210000002966 serum Anatomy 0.000 description 1
229910052708 sodium Inorganic materials 0.000 description 1
239000011734 sodium Substances 0.000 description 1
239000001509 sodium citrate Substances 0.000 description 1
NLJMYIDDQXHKNR-UHFFFAOYSA-K sodium citrate Chemical compound O.O.[Na+].[Na+].[Na+].[O-]C(=O)CC(O)(CC([O-])=O)C([O-])=O NLJMYIDDQXHKNR-UHFFFAOYSA-K 0.000 description 1
239000001540 sodium lactate Substances 0.000 description 1
235000011088 sodium lactate Nutrition 0.000 description 1
229940005581 sodium lactate Drugs 0.000 description 1
239000011684 sodium molybdate Substances 0.000 description 1
235000015393 sodium molybdate Nutrition 0.000 description 1
TVXXNOYZHKPKGW-UHFFFAOYSA-N sodium molybdate (anhydrous) Chemical compound [Na+].[Na+].[O-][Mo]([O-])(=O)=O TVXXNOYZHKPKGW-UHFFFAOYSA-N 0.000 description 1
239000007787 solid Substances 0.000 description 1
238000011895 specific detection Methods 0.000 description 1
238000010561 standard procedure Methods 0.000 description 1
230000001954 sterilising effect Effects 0.000 description 1
238000004659 sterilization and disinfection Methods 0.000 description 1
LSNNMFCWUKXFEE-UHFFFAOYSA-L sulfite Chemical compound [O-]S([O-])=O LSNNMFCWUKXFEE-UHFFFAOYSA-L 0.000 description 1
229910052717 sulfur Inorganic materials 0.000 description 1
239000011593 sulfur Substances 0.000 description 1
239000006228 supernatant Substances 0.000 description 1
239000000725 suspension Substances 0.000 description 1
230000002195 synergetic effect Effects 0.000 description 1
ABZLKHKQJHEPAX-UHFFFAOYSA-N tetramethylrhodamine Chemical compound C=12C=CC(N(C)C)=CC2=[O+]C2=CC(N(C)C)=CC=C2C=1C1=CC=CC=C1C([O-])=O ABZLKHKQJHEPAX-UHFFFAOYSA-N 0.000 description 1
230000001988 toxicity Effects 0.000 description 1
231100000419 toxicity Toxicity 0.000 description 1
238000012549 training Methods 0.000 description 1
241001300301 uncultured bacterium Species 0.000 description 1
239000010863 uranium mill tailing Substances 0.000 description 1
238000005406 washing Methods 0.000 description 1
239000002699 waste material Substances 0.000 description 1
239000012138 yeast extract Substances 0.000 description 1

Classifications

- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6806—Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6844—Nucleic acid amplification reactions
- C12Q1/686—Polymerase chain reaction [PCR]
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
- C12Q1/6888—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms
- C12Q1/689—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms for bacteria

Landscapes

Chemical & Material Sciences (AREA)
Life Sciences & Earth Sciences (AREA)
Organic Chemistry (AREA)
Proteomics, Peptides & Aminoacids (AREA)
Analytical Chemistry (AREA)
Zoology (AREA)
Wood Science & Technology (AREA)
Health & Medical Sciences (AREA)
Engineering & Computer Science (AREA)
Microbiology (AREA)
Immunology (AREA)
Molecular Biology (AREA)
Biotechnology (AREA)
Biophysics (AREA)
Physics & Mathematics (AREA)
Biochemistry (AREA)
Bioinformatics & Cheminformatics (AREA)
General Engineering & Computer Science (AREA)
General Health & Medical Sciences (AREA)
Genetics & Genomics (AREA)
Chemical Kinetics & Catalysis (AREA)
Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

CA002673577A 2006-10-17 2007-10-17 Detection de procaryotes induisant une corrosion Abandoned CA2673577A1 (fr)

Applications Claiming Priority (5)

Application Number	Priority Date	Filing Date	Title
US85222706P	2006-10-17	2006-10-17
US60/852,227		2006-10-17
US92543507P	2007-04-20	2007-04-20
US60/925,435		2007-04-20
PCT/US2007/022147 WO2008066614A2 (fr)	2006-10-17	2007-10-17	Détection de procaryotes induisant une corrosion

Publications (1)

Publication Number	Publication Date
CA2673577A1 true CA2673577A1 (fr)	2008-06-05

Family

ID=39402651

Family Applications (1)

Application Number	Title	Priority Date	Filing Date
CA002673577A Abandoned CA2673577A1 (fr)	2006-10-17	2007-10-17	Detection de procaryotes induisant une corrosion

Country Status (3)

Country	Link
CA (1)	CA2673577A1 (fr)
GB (1)	GB2456116A (fr)
WO (1)	WO2008066614A2 (fr)

Families Citing this family (1)

* Cited by examiner, † Cited by third party
Publication number	Priority date	Publication date	Assignee	Title
MX2020009985A (es) *	2018-03-26	2020-10-12	Buckman Laboratories Int Inc	Metodos para cuantificar la carga biologica en sustancias.

Family Cites Families (1)

* Cited by examiner, † Cited by third party
Publication number	Priority date	Publication date	Assignee	Title
ITMI20022479A1 (it) *	2002-11-22	2004-05-23	Enitecnologie Spa	Metodo per l'identificazione di batteri solforiduttori.

2007
- 2007-10-17 CA CA002673577A patent/CA2673577A1/fr not_active Abandoned
- 2007-10-17 WO PCT/US2007/022147 patent/WO2008066614A2/fr not_active Ceased
2009
- 2009-05-14 GB GB0908301A patent/GB2456116A/en not_active Withdrawn

Also Published As

Publication number	Publication date
WO2008066614A3 (fr)	2008-09-12
GB0908301D0 (en)	2009-06-24
GB2456116A (en)	2009-07-08
WO2008066614A2 (fr)	2008-06-05

Legal Events

Date	Code	Title	Description
2010-10-18	FZDE	Discontinued

Publication	Publication Date	Title
Lemarchand et al.	2004	Molecular biology and DNA microarray technology for microbial quality monitoring of water
Shen et al.	2013	Denitrification performance and microbial diversity in a packed-bed bioreactor using biodegradable polymer as carbon source and biofilm support
Schwartz et al.	1998	Formation and bacterial composition of young, natural biofilms obtained from public bank-filtered drinking water systems
Lin et al.	2016	Bacterial regrowth in water reclamation and distribution systems revealed by viable bacterial detection assays
JP2004533204A (ja)	2004-11-04	水性媒体の微生物学的品質の管理方法及びそれ用のキット
CN106661625B (zh)	2021-10-26	检测不存在微生物的方法和试剂盒
NO326359B1 (no)	2008-11-17	Fremgangsmate ved pavisning av forskjellige nukleotidsekvenser i en enkelt prove samt sett for utforelse av fremgangsmaten
CN104169436A (zh)	2014-11-26	革兰氏阴性菌中负责对碳青霉烯高水平抗性的kpc基因的扩增和检测的分子测定
Oude Elferink et al.	1998	Detection and quantification of microorganisms in anaerobic bioreactors
Zhu et al.	2017	Molecular microbiology techniques
Sunar et al.	2014	Molecular techniques to characterize the invA genes of Salmonella spp. for pathogen inactivation study in composting
EP1563101B1 (fr)	2008-04-23	Procede d'identification de bacteries sulfo-reductrices
WO2011116497A1 (fr)	2011-09-29	Compositions et procédés pour la détection rapide de legionella pneumophila
US20170218433A1 (en)	2017-08-03	Pcr amplification methods for detecting and quantifying sulfate-reducing bacteria in oilfield fluids
WO2008066614A2 (fr)	2008-06-05	Détection de procaryotes induisant une corrosion
Gillespie	2016	Current status of molecular microbiological techniques for the analysis of drinking water
Leach et al.	2009	Detection and enumeration of haloacetic acid‐degrading bacteria in drinking water distribution systems using dehalogenase genes
Shanker et al.	2023	Novel primer designing and PCR-AFLP approach for an expeditious detection of coliforms in potable waters
Liu et al.	2012	Biotreatment of o-nitrobenzaldehyde manufacturing wastewater and changes in activated sludge flocs in a sequencing batch reactor
WO2022229690A1 (fr)	2022-11-03	Amorces pour la détection différentielle de coliformes fécaux dans un échantillon d'eau par pcr multiplex et leurs utilisations
Lobato et al.	2026	Development of High-Specificity Primers for the Detection of Sulfate-Reducing Bacteria from the Petroleum Environment Through qPCR
US20160265035A1 (en)	2016-09-15	Pcr amplification methods, primers, and probes for detecting and quantifying sulfate-reducing bacteria
US10501790B2 (en)	2019-12-10	PCR amplification methods for detecting and quantifying sulfate-reducing bacteria in oilfield fluids
Hassan et al.	2024	Phycoremediation Efficacy of Different Microalgae Species in Treating Poultry Slaughterhouse Wastewater in Bauchi Local Government Area, Bauchi State, Nigeria
JP3861140B2 (ja)	2006-12-20	複合生物系試料における硫酸還元菌を特異的に検出するためのオリゴクレオチドプライマー