CA2712213C - Methode pour realiser une separation bidimensionnelle limitee de proteines et d'autres matieres biologiques - Google Patents

Methode pour realiser une separation bidimensionnelle limitee de proteines et d'autres matieres biologiques Download PDF

Info

Publication number
CA2712213C
CA2712213C CA2712213A CA2712213A CA2712213C CA 2712213 C CA2712213 C CA 2712213C CA 2712213 A CA2712213 A CA 2712213A CA 2712213 A CA2712213 A CA 2712213A CA 2712213 C CA2712213 C CA 2712213C
Authority
CA
Canada
Prior art keywords
proteins
protein
separation
zone
separation stage
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Active
Application number
CA2712213A
Other languages
English (en)
Other versions
CA2712213A1 (fr
Inventor
Tiemin Huang
Jiaqi Wu
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
ProteinSimple
Original Assignee
ProteinSimple
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by ProteinSimple filed Critical ProteinSimple
Priority to CA2712213A priority Critical patent/CA2712213C/fr
Priority to CA3123719A priority patent/CA3123719A1/fr
Publication of CA2712213A1 publication Critical patent/CA2712213A1/fr
Application granted granted Critical
Publication of CA2712213C publication Critical patent/CA2712213C/fr
Active legal-status Critical Current
Anticipated expiration legal-status Critical

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
    • G01N30/02—Column chromatography
    • G01N30/88—Integrated analysis systems specially adapted therefor, not covered by a single one of the groups G01N30/04 - G01N30/86
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N27/00—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
    • G01N27/26—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating electrochemical variables; by using electrolysis or electrophoresis
    • G01N27/416—Systems
    • G01N27/447—Systems using electrophoresis
    • G01N27/44704—Details; Accessories
    • G01N27/44717—Arrangements for investigating the separated zones, e.g. localising zones
    • G01N27/44721—Arrangements for investigating the separated zones, e.g. localising zones by optical means
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N27/00—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
    • G01N27/26—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating electrochemical variables; by using electrolysis or electrophoresis
    • G01N27/416—Systems
    • G01N27/447—Systems using electrophoresis
    • G01N27/44756—Apparatus specially adapted therefor
    • G01N27/44795—Isoelectric focusing
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
    • G01N30/02—Column chromatography
    • G01N30/62—Detectors specially adapted therefor
    • G01N30/74—Optical detectors
    • G01N2030/746—Optical detectors detecting along the line of flow, e.g. axial
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
    • G01N30/02—Column chromatography
    • G01N30/88—Integrated analysis systems specially adapted therefor, not covered by a single one of the groups G01N30/04 - G01N30/86
    • G01N2030/8809—Integrated analysis systems specially adapted therefor, not covered by a single one of the groups G01N30/04 - G01N30/86 analysis specially adapted for the sample
    • G01N2030/8813—Integrated analysis systems specially adapted therefor, not covered by a single one of the groups G01N30/04 - G01N30/86 analysis specially adapted for the sample biological materials
    • G01N2030/8831—Integrated analysis systems specially adapted therefor, not covered by a single one of the groups G01N30/04 - G01N30/86 analysis specially adapted for the sample biological materials involving peptides or proteins

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Molecular Biology (AREA)
  • Physics & Mathematics (AREA)
  • Analytical Chemistry (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • General Physics & Mathematics (AREA)
  • Immunology (AREA)
  • Pathology (AREA)
  • Electrochemistry (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Peptides Or Proteins (AREA)
  • Investigating Or Analysing Biological Materials (AREA)

Abstract

Un procédé et un appareil permettent deffectuer une focalisation isoélectrique capillaire suivie dune mobilisation des zones focalisées par un écoulement hydrodynamique induit ou une mobilisation chimique. Ces deux dimensions de séparation sont intégrées à une détection délectrophorèse sur un canal entier en temps réel et une injection déchantillon automatique pour obtenir une résolution de séparation supérieure à celle pouvant être obtenue à laide dagencements bidimensionnels capillaires orthogonaux connus.
CA2712213A 2010-08-05 2010-08-05 Methode pour realiser une separation bidimensionnelle limitee de proteines et d'autres matieres biologiques Active CA2712213C (fr)

Priority Applications (2)

Application Number Priority Date Filing Date Title
CA2712213A CA2712213C (fr) 2010-08-05 2010-08-05 Methode pour realiser une separation bidimensionnelle limitee de proteines et d'autres matieres biologiques
CA3123719A CA3123719A1 (fr) 2010-08-05 2010-08-05 Methode pour realiser une separation bidimensionnelle limitee de proteines et d'autres matieres biologiques

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
CA2712213A CA2712213C (fr) 2010-08-05 2010-08-05 Methode pour realiser une separation bidimensionnelle limitee de proteines et d'autres matieres biologiques

Related Child Applications (1)

Application Number Title Priority Date Filing Date
CA3123719A Division CA3123719A1 (fr) 2010-08-05 2010-08-05 Methode pour realiser une separation bidimensionnelle limitee de proteines et d'autres matieres biologiques

Publications (2)

Publication Number Publication Date
CA2712213A1 CA2712213A1 (fr) 2012-02-05
CA2712213C true CA2712213C (fr) 2021-08-24

Family

ID=45566691

Family Applications (2)

Application Number Title Priority Date Filing Date
CA2712213A Active CA2712213C (fr) 2010-08-05 2010-08-05 Methode pour realiser une separation bidimensionnelle limitee de proteines et d'autres matieres biologiques
CA3123719A Pending CA3123719A1 (fr) 2010-08-05 2010-08-05 Methode pour realiser une separation bidimensionnelle limitee de proteines et d'autres matieres biologiques

Family Applications After (1)

Application Number Title Priority Date Filing Date
CA3123719A Pending CA3123719A1 (fr) 2010-08-05 2010-08-05 Methode pour realiser une separation bidimensionnelle limitee de proteines et d'autres matieres biologiques

Country Status (1)

Country Link
CA (2) CA2712213C (fr)

Also Published As

Publication number Publication date
CA2712213A1 (fr) 2012-02-05
CA3123719A1 (fr) 2012-02-05

Similar Documents

Publication Publication Date Title
US10107782B2 (en) Method to perform limited two dimensional separation of proteins and other biologicals
CA2657317C (fr) Procede et appareil permettant de selectionner et d'extraire precisement un element focalise dans des micro-canaux realises par focalisation isoelectrique
JP7570403B2 (ja) 等電点電気泳動デバイスおよび固定具
US10866246B2 (en) Devices, methods and kits for sample characterization
JP6422131B2 (ja) 分離分析用キャピラリーデバイス、分離分析用マイクロ流体チップ、タンパク質又はペプチド分析方法、電気泳動装置、及び分離分析用マイクロ流体チップ電気泳動装置
US20130213811A1 (en) Microscale western blot
EP3226993B1 (fr) Appareil et procédé pour séparer des molécules
Bergmann et al. Potential of on-line isotachophoresis-capillary zone electrophoresis with hydrodynamic counterflow in the analysis of various basic proteins and recombinant human interleukin-3
US20130140182A1 (en) PROTEIN FRACTIONATION BASED ON pI
CA2712213C (fr) Methode pour realiser une separation bidimensionnelle limitee de proteines et d'autres matieres biologiques
US12594557B2 (en) Isoelectric focusing devices and fixtures
Jenkins Clinical applications of capillary electrophoresis: Status at the new millennium
Wu et al. Analysis of proteins by CE, CIEF, and microfluidic devices with whole-column-imaging detection
Mikuš et al. Column coupling electrophoresis in biomedical analysis
Schwartz et al. Separation of peptides and proteins
Zhan Development of Isoelectric Focusing Techniques for Protein Analyses
Barahona Development of a microfluidic, segmented-flow, single molecule, enzyme activity assay and improvement of separation efficiency of basic proteins by application of a waterproofing agent as a coating in capillary electrophoresis
Kilár Capillary Isoelectric Focusing
Castro Barahona Development of a microfluidic, segmented-flow, single molecule, enzyme activity assay and improvement of separation efficiency of basic proteins by application of a water-proofing agent as a coating in capillary electrophoresis

Legal Events

Date Code Title Description
EEER Examination request

Effective date: 20150707

MPN Maintenance fee for patent paid

Free format text: FEE DESCRIPTION TEXT: MF (PATENT, 14TH ANNIV.) - STANDARD

Year of fee payment: 14

U00 Fee paid

Free format text: ST27 STATUS EVENT CODE: A-4-4-U10-U00-U101 (AS PROVIDED BY THE NATIONAL OFFICE); EVENT TEXT: MAINTENANCE REQUEST RECEIVED

Effective date: 20240726

U11 Full renewal or maintenance fee paid

Free format text: ST27 STATUS EVENT CODE: A-4-4-U10-U11-U102 (AS PROVIDED BY THE NATIONAL OFFICE); EVENT TEXT: MAINTENANCE FEE PAYMENT PAID IN FULL

Effective date: 20240726

MPN Maintenance fee for patent paid

Free format text: FEE DESCRIPTION TEXT: MF (PATENT, 15TH ANNIV.) - STANDARD

Year of fee payment: 15

U00 Fee paid

Free format text: ST27 STATUS EVENT CODE: A-4-4-U10-U00-U101 (AS PROVIDED BY THE NATIONAL OFFICE); EVENT TEXT: MAINTENANCE REQUEST RECEIVED

Effective date: 20250801

U11 Full renewal or maintenance fee paid

Free format text: ST27 STATUS EVENT CODE: A-4-4-U10-U11-U102 (AS PROVIDED BY THE NATIONAL OFFICE); EVENT TEXT: MAINTENANCE FEE PAYMENT PAID IN FULL

Effective date: 20250829