CA2720460C - Procedes et dispositifs bases sur une nouvelle forme de duplex d'acides nucleiques sur une surface - Google Patents
Procedes et dispositifs bases sur une nouvelle forme de duplex d'acides nucleiques sur une surface Download PDFInfo
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- CA2720460C CA2720460C CA2720460A CA2720460A CA2720460C CA 2720460 C CA2720460 C CA 2720460C CA 2720460 A CA2720460 A CA 2720460A CA 2720460 A CA2720460 A CA 2720460A CA 2720460 C CA2720460 C CA 2720460C
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- oligonucleotide
- hybridization device
- biomolecular hybridization
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- oligonucleotides
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- B01J19/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
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- B—PERFORMING OPERATIONS; TRANSPORTING
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Landscapes
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
Abstract
L'invention porte sur des dispositifs d'hybridation biomoléculaire comprenant un substrat avec une surface attachée de manière permanente et covalente de groupes fonctionnels et une monocouche adsorbée d'oligonucléotides simple brin non modifiés, dont la totalité sont de 10 à environ 24 bases de longueur en tant que film saturé d'oligonucléotides contraints sur la surface par l'intermédiaire d'un contact adsorptif de surface de phosphate non covalent direct de sensiblement tous les groupes phosphate de chaque oligonucléotide. Les oligonucléotides contraints sont efficaces pour s'hybrider de manière dissociable à un acide nucléique simple brin complémentaire avec un appariement de bases asymétrique, non hélicoïdal et sans dissociation des oligonucléotides à partir de la surface du dispositif. L'invention porte également sur des procédés pour hybrider les acides nucléiques cibles à l'état de solution à des acides nucléiques sondes et pour identifier une séquence nucléotidique à laquelle une protéine de liaison à un nucléotide se lie à l'aide des dispositifs d'hybridation biomoléculaire.
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US12/080,720 US10272409B2 (en) | 2001-07-11 | 2008-04-04 | Methods and devices based upon a novel form of nucleic acid duplex on a surface |
| US12/080,720 | 2008-04-04 | ||
| PCT/US2009/002088 WO2009145836A2 (fr) | 2008-04-04 | 2009-04-03 | Procédés et dispositifs basés sur une nouvelle forme de duplex d'acides nucléiques sur une surface |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CA2720460A1 CA2720460A1 (fr) | 2009-12-03 |
| CA2720460C true CA2720460C (fr) | 2019-10-29 |
Family
ID=41377835
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CA2720460A Expired - Fee Related CA2720460C (fr) | 2008-04-04 | 2009-04-03 | Procedes et dispositifs bases sur une nouvelle forme de duplex d'acides nucleiques sur une surface |
Country Status (6)
| Country | Link |
|---|---|
| US (2) | US10272409B2 (fr) |
| EP (1) | EP2283162A4 (fr) |
| JP (2) | JP2011516062A (fr) |
| AU (1) | AU2009251843B2 (fr) |
| CA (1) | CA2720460C (fr) |
| WO (1) | WO2009145836A2 (fr) |
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| JP2010510247A (ja) * | 2006-11-21 | 2010-04-02 | アプライド バイオシステムズ インコーポレイテッド | 酸化物層上に不動態化表面を形成するための中間体および方法およびこれにより製造される製品 |
| US8173198B2 (en) * | 2008-07-23 | 2012-05-08 | Life Technologies Corporation | Deposition of metal oxides onto surfaces as an immobilization vehicle for carboxylated or phophated particles or polymers |
| US8771951B2 (en) | 2009-11-16 | 2014-07-08 | Genomics Usa, Inc. | Methods for PCR and HLA typing using raw blood |
| US11851705B2 (en) | 2015-12-28 | 2023-12-26 | PathogenDX Inc | Microarray based multiplex pathogen analysis for plants, agriculture, food, and water |
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- 2009-04-03 JP JP2011502988A patent/JP2011516062A/ja active Pending
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- 2009-04-03 AU AU2009251843A patent/AU2009251843B2/en not_active Ceased
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| Publication number | Publication date |
|---|---|
| JP6185958B2 (ja) | 2017-08-23 |
| JP2011516062A (ja) | 2011-05-26 |
| CA2720460A1 (fr) | 2009-12-03 |
| US10888830B2 (en) | 2021-01-12 |
| US20190209996A1 (en) | 2019-07-11 |
| WO2009145836A2 (fr) | 2009-12-03 |
| EP2283162A4 (fr) | 2012-06-27 |
| US10272409B2 (en) | 2019-04-30 |
| AU2009251843A1 (en) | 2009-12-03 |
| US20090011949A1 (en) | 2009-01-08 |
| WO2009145836A3 (fr) | 2010-01-21 |
| WO2009145836A8 (fr) | 2010-11-04 |
| AU2009251843B2 (en) | 2015-12-03 |
| JP2015180206A (ja) | 2015-10-15 |
| EP2283162A2 (fr) | 2011-02-16 |
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