CA2726850C - Procede de synthese de variants de polynucleotides - Google Patents

Procede de synthese de variants de polynucleotides Download PDF

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Publication number
CA2726850C
CA2726850C CA2726850A CA2726850A CA2726850C CA 2726850 C CA2726850 C CA 2726850C CA 2726850 A CA2726850 A CA 2726850A CA 2726850 A CA2726850 A CA 2726850A CA 2726850 C CA2726850 C CA 2726850C
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sequence
polynucleotide
amplicons
amino acid
variants
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CA2726850A1 (fr
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Jeffrey Colbeck
Benjamin Mijts
Lorraine Jean Giver
Richard J. Fox
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Codexis Inc
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Codexis Inc
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/102—Mutagenizing nucleic acids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/102—Mutagenizing nucleic acids
    • C12N15/1031—Mutagenizing nucleic acids mutagenesis by gene assembly, e.g. assembly by oligonucleotide extension PCR
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034—Isolating an individual clone by screening libraries
    • C12N15/1093—General methods of preparing gene libraries, not provided for in other subgroups
    • C—CHEMISTRY; METALLURGY
    • C40—COMBINATORIAL TECHNOLOGY
    • C40B—COMBINATORIAL CHEMISTRY; LIBRARIES, e.g. CHEMICAL LIBRARIES
    • C40B40/00—Libraries per se, e.g. arrays, mixtures
    • C40B40/04—Libraries containing only organic compounds
    • C40B40/06—Libraries containing nucleotides or polynucleotides, or derivatives thereof
    • C—CHEMISTRY; METALLURGY
    • C40—COMBINATORIAL TECHNOLOGY
    • C40B—COMBINATORIAL CHEMISTRY; LIBRARIES, e.g. CHEMICAL LIBRARIES
    • C40B50/00—Methods of creating libraries, e.g. combinatorial synthesis
    • C40B50/06—Biochemical methods, e.g. using enzymes or whole viable microorganisms

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  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biomedical Technology (AREA)
  • Wood Science & Technology (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biochemistry (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • Plant Pathology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

La présente invention porte sur des procédés pour générer des bibliothèques de variants de polynucléotides comprenant des différences de nucléotides définies par rapport à un polynucléotide de référence.
CA2726850A 2008-06-13 2009-06-11 Procede de synthese de variants de polynucleotides Active CA2726850C (fr)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US6158108P 2008-06-13 2008-06-13
US61/061,581 2008-06-13
PCT/US2009/047046 WO2009152336A1 (fr) 2008-06-13 2009-06-11 Procédé de synthèse de variants de polynucléotides

Publications (2)

Publication Number Publication Date
CA2726850A1 CA2726850A1 (fr) 2009-12-17
CA2726850C true CA2726850C (fr) 2015-06-02

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Family Applications (1)

Application Number Title Priority Date Filing Date
CA2726850A Active CA2726850C (fr) 2008-06-13 2009-06-11 Procede de synthese de variants de polynucleotides

Country Status (7)

Country Link
EP (2) EP3023494B1 (fr)
CN (1) CN102066561B (fr)
CA (1) CA2726850C (fr)
DK (2) DK3023494T3 (fr)
ES (2) ES2705694T3 (fr)
HU (2) HUE029228T2 (fr)
WO (1) WO2009152336A1 (fr)

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Also Published As

Publication number Publication date
ES2705694T3 (es) 2019-03-26
CN102066561A (zh) 2011-05-18
EP2285958A4 (fr) 2012-02-01
HUE041367T2 (hu) 2019-05-28
WO2009152336A1 (fr) 2009-12-17
CN102066561B (zh) 2013-09-25
CA2726850A1 (fr) 2009-12-17
EP2285958B1 (fr) 2016-03-09
EP3023494A1 (fr) 2016-05-25
EP2285958A1 (fr) 2011-02-23
HUE029228T2 (en) 2017-02-28
DK2285958T3 (en) 2016-03-29
DK3023494T3 (en) 2019-02-25
ES2575005T3 (es) 2016-06-23
EP3023494B1 (fr) 2018-11-28

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