CA2809828C - Element regulateur pour la production de proteines heterologues dans l'organe fructifere de champignons filamenteux - Google Patents
Element regulateur pour la production de proteines heterologues dans l'organe fructifere de champignons filamenteux Download PDFInfo
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- CA2809828C CA2809828C CA2809828A CA2809828A CA2809828C CA 2809828 C CA2809828 C CA 2809828C CA 2809828 A CA2809828 A CA 2809828A CA 2809828 A CA2809828 A CA 2809828A CA 2809828 C CA2809828 C CA 2809828C
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Classifications
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/37—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from fungi
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/80—Vectors or expression systems specially adapted for eukaryotic hosts for fungi
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Genetics & Genomics (AREA)
- Organic Chemistry (AREA)
- Engineering & Computer Science (AREA)
- Biophysics (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Biochemistry (AREA)
- Zoology (AREA)
- Molecular Biology (AREA)
- Wood Science & Technology (AREA)
- General Health & Medical Sciences (AREA)
- General Engineering & Computer Science (AREA)
- Mycology (AREA)
- Biomedical Technology (AREA)
- Biotechnology (AREA)
- Physics & Mathematics (AREA)
- Plant Pathology (AREA)
- Gastroenterology & Hepatology (AREA)
- Microbiology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Medicinal Chemistry (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Abstract
L'invention concerne des compositions et des méthodes pouvant réguler l'expression de séquences nucléotidiques chez des champignons. Ces compositions sont de nouvelles séquences nucléotidiques pour un promoteur à préférence tissulaire, isolé du gène de la lectine d'Agaricus bisporus. Ces séquences s'expriment, de préférence, dans le tissu de l'organe fructifère. L'invention concerne également une méthode utilisant ces séquences régulatrices pour exprimer une séquence nucléotidique chez des champignons. La méthode consiste à transformer une cellule fongique de sorte qu'elle comprenne une séquence nucléotidique liée de manière fonctionnelle à une ou plusieurs des séquences régulatrices de la présente invention; et à régénérer un champignon transformé de manière stable à partir de la cellule transformée.
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US87092010A | 2010-08-30 | 2010-08-30 | |
| US12/870,920 | 2010-08-30 | ||
| PCT/US2011/049741 WO2012030827A1 (fr) | 2010-08-30 | 2011-08-30 | Élément régulateur pour la production de protéines hétérologues dans l'organe fructifère de champignons filamenteux |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CA2809828A1 CA2809828A1 (fr) | 2012-03-08 |
| CA2809828C true CA2809828C (fr) | 2019-01-29 |
Family
ID=45773239
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CA2809828A Expired - Fee Related CA2809828C (fr) | 2010-08-30 | 2011-08-30 | Element regulateur pour la production de proteines heterologues dans l'organe fructifere de champignons filamenteux |
Country Status (3)
| Country | Link |
|---|---|
| CN (1) | CN103180433B (fr) |
| CA (1) | CA2809828C (fr) |
| WO (1) | WO2012030827A1 (fr) |
Families Citing this family (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US10131916B2 (en) | 2010-08-30 | 2018-11-20 | Intrexon Corporation | Regulatory element for heterologous protein production in the fruiting body of filamentous fungi |
| CN104611337B (zh) * | 2015-02-21 | 2018-02-13 | 吉林农业大学 | 低温诱导型启动子pcp1及应用 |
| CN105602953A (zh) * | 2016-03-09 | 2016-05-25 | 浙江农林大学 | 双孢蘑菇诱导型启动子和表达载体及其应用 |
| CN107090020B (zh) * | 2017-06-09 | 2020-10-16 | 福建省平潭县水产良种实验有限公司 | 食用菌凝集素及其在抑制棕囊藻生长中的应用 |
Family Cites Families (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US6555655B1 (en) * | 1999-05-04 | 2003-04-29 | Monsanto Technology, Llc | Coleopteran-toxic polypeptide compositions and insect-resistant transgenic plants |
| GB0225390D0 (en) * | 2002-10-31 | 2002-12-11 | Horticulture Res Internat | Selective expression in filamentous fungi |
| US8012706B2 (en) * | 2005-05-03 | 2011-09-06 | Institut Pasteur | Methods for detecting virulent Plasmodium, for evaluating Plasmodium virulence, and for screening new drugs employing the 3′UTR of Plasmodium SUB2 and the Plasmodium SUB2 serine protease |
| CN101440377A (zh) * | 2008-11-11 | 2009-05-27 | 中国热带农业科学院热带生物技术研究所 | 一种在果实中特异表达白藜芦醇的植物表达载体 |
-
2011
- 2011-08-30 WO PCT/US2011/049741 patent/WO2012030827A1/fr not_active Ceased
- 2011-08-30 CA CA2809828A patent/CA2809828C/fr not_active Expired - Fee Related
- 2011-08-30 CN CN201180051472.4A patent/CN103180433B/zh not_active Expired - Fee Related
Also Published As
| Publication number | Publication date |
|---|---|
| WO2012030827A1 (fr) | 2012-03-08 |
| CN103180433A (zh) | 2013-06-26 |
| CN103180433B (zh) | 2015-05-13 |
| CA2809828A1 (fr) | 2012-03-08 |
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