CA2846690A1 - Polypeptides ayant une activite glucoamylase et polynucleotides codant pour ces polypeptides - Google Patents

Polypeptides ayant une activite glucoamylase et polynucleotides codant pour ces polypeptides Download PDF

Info

Publication number: CA2846690A1
Authority: CA; Canada
Prior art keywords: polypeptide; seq; polynucleotide; starch; alpha
Prior art date: 2011-08-26
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.): Abandoned

Application number

CA2846690A

Other languages

English (en)

Inventor

Tianqi Sun

Ming Li

Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)

Novozymes AS

Original Assignee

Novozymes AS

Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)

2011-08-26

Filing date

2012-08-24

Publication date

2013-03-07

2012-08-24 Application filed by Novozymes AS filed Critical Novozymes AS

2013-03-07 Publication of CA2846690A1 publication Critical patent/CA2846690A1/fr

Status Abandoned legal-status Critical Current

Links

108090000765 processed proteins & peptides Proteins 0.000 title claims abstract description 267
102000004196 processed proteins & peptides Human genes 0.000 title claims abstract description 265
229920001184 polypeptide Polymers 0.000 title claims abstract description 264
102100022624 Glucoamylase Human genes 0.000 title claims abstract description 102
108010073178 Glucan 1,4-alpha-Glucosidase Proteins 0.000 title claims abstract description 99
108091033319 polynucleotide Proteins 0.000 title claims abstract description 98
102000040430 polynucleotide Human genes 0.000 title claims abstract description 98
239000002157 polynucleotide Substances 0.000 title claims abstract description 98
230000000694 effects Effects 0.000 title claims abstract description 77
238000000034 method Methods 0.000 claims abstract description 111
150000007523 nucleic acids Chemical class 0.000 claims abstract description 23
102000039446 nucleic acids Human genes 0.000 claims abstract description 22
108020004707 nucleic acids Proteins 0.000 claims abstract description 22
229920002472 Starch Polymers 0.000 claims description 86
239000008107 starch Substances 0.000 claims description 86
235000019698 starch Nutrition 0.000 claims description 86
108090000637 alpha-Amylases Proteins 0.000 claims description 76
102000004139 alpha-Amylases Human genes 0.000 claims description 69
150000001413 amino acids Chemical class 0.000 claims description 63
229940024171 alpha-amylase Drugs 0.000 claims description 62
238000000855 fermentation Methods 0.000 claims description 59
230000004151 fermentation Effects 0.000 claims description 59
239000000463 material Substances 0.000 claims description 50
239000002773 nucleotide Substances 0.000 claims description 47
125000003729 nucleotide group Chemical group 0.000 claims description 47
108091026890 Coding region Proteins 0.000 claims description 45
230000003197 catalytic effect Effects 0.000 claims description 41
FWMNVWWHGCHHJJ-SKKKGAJSSA-N 4-amino-1-[(2r)-6-amino-2-[[(2r)-2-[[(2r)-2-[[(2r)-2-amino-3-phenylpropanoyl]amino]-3-phenylpropanoyl]amino]-4-methylpentanoyl]amino]hexanoyl]piperidine-4-carboxylic acid Chemical compound C([C@H](C(=O)N[C@H](CC(C)C)C(=O)N[C@H](CCCCN)C(=O)N1CCC(N)(CC1)C(O)=O)NC(=O)[C@H](N)CC=1C=CC=CC=1)C1=CC=CC=C1 FWMNVWWHGCHHJJ-SKKKGAJSSA-N 0.000 claims description 38
230000008569 process Effects 0.000 claims description 37
239000002299 complementary DNA Substances 0.000 claims description 32
239000000203 mixture Substances 0.000 claims description 31
238000006467 substitution reaction Methods 0.000 claims description 28
238000004519 manufacturing process Methods 0.000 claims description 25
238000012217 deletion Methods 0.000 claims description 23
230000037430 deletion Effects 0.000 claims description 23
239000012634 fragment Substances 0.000 claims description 20
238000003780 insertion Methods 0.000 claims description 20
230000037431 insertion Effects 0.000 claims description 20
108010038196 saccharide-binding proteins Proteins 0.000 claims description 19
239000013604 expression vector Substances 0.000 claims description 15
239000006188 syrup Substances 0.000 claims description 12
235000020357 syrup Nutrition 0.000 claims description 12
230000000295 complement effect Effects 0.000 claims description 10
230000009261 transgenic effect Effects 0.000 claims description 10
239000007858 starting material Substances 0.000 claims description 6
239000013598 vector Substances 0.000 abstract description 36
108020001778 catalytic domains Proteins 0.000 abstract description 13
210000004027 cell Anatomy 0.000 description 131
108090000623 proteins and genes Proteins 0.000 description 81
241000196324 Embryophyta Species 0.000 description 71
235000001014 amino acid Nutrition 0.000 description 64
LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 description 58
229940024606 amino acid Drugs 0.000 description 57
102000004190 Enzymes Human genes 0.000 description 55
108090000790 Enzymes Proteins 0.000 description 55
229940088598 enzyme Drugs 0.000 description 55
239000000047 product Substances 0.000 description 47
108020004414 DNA Proteins 0.000 description 46
240000004808 Saccharomyces cerevisiae Species 0.000 description 41
235000014680 Saccharomyces cerevisiae Nutrition 0.000 description 41
WQZGKKKJIJFFOK-VFUOTHLCSA-N beta-D-glucose Chemical compound OC[C@H]1O[C@@H](O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-VFUOTHLCSA-N 0.000 description 38
WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 description 36
230000002538 fungal effect Effects 0.000 description 32
239000008103 glucose Substances 0.000 description 29
108010076504 Protein Sorting Signals Proteins 0.000 description 28
XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 28
241000193830 Bacillus <bacterium> Species 0.000 description 25
125000003275 alpha amino acid group Chemical group 0.000 description 23
239000000523 sample Substances 0.000 description 23
102000004169 proteins and genes Human genes 0.000 description 22
238000006243 chemical reaction Methods 0.000 description 21
239000002609 medium Substances 0.000 description 21
235000018102 proteins Nutrition 0.000 description 20
230000009466 transformation Effects 0.000 description 19
241000228245 Aspergillus niger Species 0.000 description 17
-1 H2 and CO2) Chemical class 0.000 description 17
230000010076 replication Effects 0.000 description 17
101710146708 Acid alpha-amylase Proteins 0.000 description 16
239000004382 Amylase Substances 0.000 description 16
230000001580 bacterial effect Effects 0.000 description 16
239000013612 plasmid Substances 0.000 description 15
238000003752 polymerase chain reaction Methods 0.000 description 15
239000000243 solution Substances 0.000 description 15
239000000126 substance Substances 0.000 description 15
239000002002 slurry Substances 0.000 description 14
108010065511 Amylases Proteins 0.000 description 13
240000006439 Aspergillus oryzae Species 0.000 description 13
235000002247 Aspergillus oryzae Nutrition 0.000 description 13
239000008186 active pharmaceutical agent Substances 0.000 description 13
238000003556 assay Methods 0.000 description 13
102000013142 Amylases Human genes 0.000 description 12
ZHNUHDYFZUAESO-UHFFFAOYSA-N Formamide Chemical compound NC=O ZHNUHDYFZUAESO-UHFFFAOYSA-N 0.000 description 12
241000223218 Fusarium Species 0.000 description 12
241000499912 Trichoderma reesei Species 0.000 description 12
235000019418 amylase Nutrition 0.000 description 12
UHPMCKVQTMMPCG-UHFFFAOYSA-N 5,8-dihydroxy-2-methoxy-6-methyl-7-(2-oxopropyl)naphthalene-1,4-dione Chemical compound CC1=C(CC(C)=O)C(O)=C2C(=O)C(OC)=CC(=O)C2=C1O UHPMCKVQTMMPCG-UHFFFAOYSA-N 0.000 description 11
QTBSBXVTEAMEQO-UHFFFAOYSA-N Acetic acid Chemical compound CC(O)=O QTBSBXVTEAMEQO-UHFFFAOYSA-N 0.000 description 11
239000008367 deionised water Substances 0.000 description 11
229910021641 deionized water Inorganic materials 0.000 description 11
210000001938 protoplast Anatomy 0.000 description 11
239000000758 substrate Substances 0.000 description 11
YBJHBAHKTGYVGT-ZKWXMUAHSA-N (+)-Biotin Chemical compound N1C(=O)N[C@@H]2[C@H](CCCCC(=O)O)SC[C@@H]21 YBJHBAHKTGYVGT-ZKWXMUAHSA-N 0.000 description 10
241000228212 Aspergillus Species 0.000 description 10
108020004711 Nucleic Acid Probes Proteins 0.000 description 10
240000007594 Oryza sativa Species 0.000 description 10
235000007164 Oryza sativa Nutrition 0.000 description 10
241000228341 Talaromyces Species 0.000 description 10
239000002853 nucleic acid probe Substances 0.000 description 10
230000001105 regulatory effect Effects 0.000 description 10
235000009566 rice Nutrition 0.000 description 10
241000894007 species Species 0.000 description 10
CSCPPACGZOOCGX-UHFFFAOYSA-N Acetone Chemical compound CC(C)=O CSCPPACGZOOCGX-UHFFFAOYSA-N 0.000 description 9
OKKJLVBELUTLKV-UHFFFAOYSA-N Methanol Chemical compound OC OKKJLVBELUTLKV-UHFFFAOYSA-N 0.000 description 9
230000027455 binding Effects 0.000 description 9
KRKNYBCHXYNGOX-UHFFFAOYSA-N citric acid Chemical compound OC(=O)CC(O)(C(O)=O)CC(O)=O KRKNYBCHXYNGOX-UHFFFAOYSA-N 0.000 description 9
238000011534 incubation Methods 0.000 description 9
OWEGMIWEEQEYGQ-UHFFFAOYSA-N 100676-05-9 Natural products OC1C(O)C(O)C(CO)OC1OCC1C(O)C(O)C(O)C(OC2C(OC(O)C(O)C2O)CO)O1 OWEGMIWEEQEYGQ-UHFFFAOYSA-N 0.000 description 8
229920001817 Agar Polymers 0.000 description 8
241000351920 Aspergillus nidulans Species 0.000 description 8
108010059892 Cellulase Proteins 0.000 description 8
241000233866 Fungi Species 0.000 description 8
108050008938 Glucoamylases Proteins 0.000 description 8
GUBGYTABKSRVRQ-PICCSMPSSA-N Maltose Natural products O[C@@H]1[C@@H](O)[C@H](O)[C@@H](CO)O[C@@H]1O[C@@H]1[C@@H](CO)OC(O)[C@H](O)[C@H]1O GUBGYTABKSRVRQ-PICCSMPSSA-N 0.000 description 8
239000002253 acid Substances 0.000 description 8
239000008272 agar Substances 0.000 description 8
239000000872 buffer Substances 0.000 description 8
239000012876 carrier material Substances 0.000 description 8
230000004927 fusion Effects 0.000 description 8
238000009396 hybridization Methods 0.000 description 8
JVTAAEKCZFNVCJ-UHFFFAOYSA-N lactic acid Chemical compound CC(O)C(O)=O JVTAAEKCZFNVCJ-UHFFFAOYSA-N 0.000 description 8
210000001519 tissue Anatomy 0.000 description 8
238000013518 transcription Methods 0.000 description 8
230000035897 transcription Effects 0.000 description 8
244000061456 Solanum tuberosum Species 0.000 description 7
235000002595 Solanum tuberosum Nutrition 0.000 description 7
108020004999 messenger RNA Proteins 0.000 description 7
230000035772 mutation Effects 0.000 description 7
239000002245 particle Substances 0.000 description 7
DLFVBJFMPXGRIB-UHFFFAOYSA-N Acetamide Chemical compound CC(N)=O DLFVBJFMPXGRIB-UHFFFAOYSA-N 0.000 description 6
101000757144 Aspergillus niger Glucoamylase Proteins 0.000 description 6
241000972773 Aulopiformes Species 0.000 description 6
235000014469 Bacillus subtilis Nutrition 0.000 description 6
241000894006 Bacteria Species 0.000 description 6
RGHNJXZEOKUKBD-SQOUGZDYSA-N D-gluconic acid Chemical compound OC[C@@H](O)[C@@H](O)[C@H](O)[C@@H](O)C(O)=O RGHNJXZEOKUKBD-SQOUGZDYSA-N 0.000 description 6
241000193385 Geobacillus stearothermophilus Species 0.000 description 6
239000004366 Glucose oxidase Substances 0.000 description 6
108010015776 Glucose oxidase Proteins 0.000 description 6
WHUUTDBJXJRKMK-UHFFFAOYSA-N Glutamic acid Natural products OC(=O)C(N)CCC(O)=O WHUUTDBJXJRKMK-UHFFFAOYSA-N 0.000 description 6
WHUUTDBJXJRKMK-VKHMYHEASA-N L-glutamic acid Chemical compound OC(=O)[C@@H](N)CCC(O)=O WHUUTDBJXJRKMK-VKHMYHEASA-N 0.000 description 6
LRHPLDYGYMQRHN-UHFFFAOYSA-N N-Butanol Chemical compound CCCCO LRHPLDYGYMQRHN-UHFFFAOYSA-N 0.000 description 6
108091028043 Nucleic acid sequence Proteins 0.000 description 6
241000959173 Rasamsonia emersonii Species 0.000 description 6
AUNGANRZJHBGPY-SCRDCRAPSA-N Riboflavin Chemical compound OC[C@@H](O)[C@@H](O)[C@@H](O)CN1C=2C=C(C)C(C)=CC=2N=C2C1=NC(=O)NC2=O AUNGANRZJHBGPY-SCRDCRAPSA-N 0.000 description 6
HEMHJVSKTPXQMS-UHFFFAOYSA-M Sodium hydroxide Chemical compound [OH-].[Na+] HEMHJVSKTPXQMS-UHFFFAOYSA-M 0.000 description 6
208000037065 Subacute sclerosing leukoencephalitis Diseases 0.000 description 6
206010042297 Subacute sclerosing panencephalitis Diseases 0.000 description 6
108010048241 acetamidase Proteins 0.000 description 6
235000013405 beer Nutrition 0.000 description 6
229940041514 candida albicans extract Drugs 0.000 description 6
235000013339 cereals Nutrition 0.000 description 6
238000004520 electroporation Methods 0.000 description 6
230000002068 genetic effect Effects 0.000 description 6
229940116332 glucose oxidase Drugs 0.000 description 6
235000019420 glucose oxidase Nutrition 0.000 description 6
239000003550 marker Substances 0.000 description 6
230000004048 modification Effects 0.000 description 6
238000012986 modification Methods 0.000 description 6
239000000843 powder Substances 0.000 description 6
235000019515 salmon Nutrition 0.000 description 6
239000012138 yeast extract Substances 0.000 description 6
244000063299 Bacillus subtilis Species 0.000 description 5
108010025880 Cyclomaltodextrin glucanotransferase Proteins 0.000 description 5
241000221779 Fusarium sambucinum Species 0.000 description 5
DHMQDGOQFOQNFH-UHFFFAOYSA-N Glycine Chemical compound NCC(O)=O DHMQDGOQFOQNFH-UHFFFAOYSA-N 0.000 description 5
125000003412 L-alanyl group Chemical group [H]N([H])[C@@](C([H])([H])[H])(C(=O)[*])[H] 0.000 description 5
241000209510 Liliopsida Species 0.000 description 5
102000004316 Oxidoreductases Human genes 0.000 description 5
108090000854 Oxidoreductases Proteins 0.000 description 5
102000003992 Peroxidases Human genes 0.000 description 5
102000012288 Phosphopyruvate Hydratase Human genes 0.000 description 5
108010022181 Phosphopyruvate Hydratase Proteins 0.000 description 5
238000002835 absorbance Methods 0.000 description 5
102000020006 aldose 1-epimerase Human genes 0.000 description 5
108091022872 aldose 1-epimerase Proteins 0.000 description 5
125000000539 amino acid group Chemical group 0.000 description 5
229960002685 biotin Drugs 0.000 description 5
235000020958 biotin Nutrition 0.000 description 5
239000011616 biotin Substances 0.000 description 5
230000008859 change Effects 0.000 description 5
239000003153 chemical reaction reagent Substances 0.000 description 5
238000010367 cloning Methods 0.000 description 5
238000009837 dry grinding Methods 0.000 description 5
150000004676 glycans Chemical class 0.000 description 5
239000008187 granular material Substances 0.000 description 5
230000010354 integration Effects 0.000 description 5
244000005700 microbiome Species 0.000 description 5
150000002482 oligosaccharides Polymers 0.000 description 5
229940072417 peroxidase Drugs 0.000 description 5
108040007629 peroxidase activity proteins Proteins 0.000 description 5
230000008488 polyadenylation Effects 0.000 description 5
229920001282 polysaccharide Polymers 0.000 description 5
239000005017 polysaccharide Substances 0.000 description 5
239000013615 primer Substances 0.000 description 5
238000003259 recombinant expression Methods 0.000 description 5
239000012266 salt solution Substances 0.000 description 5
235000000346 sugar Nutrition 0.000 description 5
238000001238 wet grinding Methods 0.000 description 5
IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 description 4
241000194108 Bacillus licheniformis Species 0.000 description 4
108010008885 Cellulose 1,4-beta-Cellobiosidase Proteins 0.000 description 4
241000588724 Escherichia coli Species 0.000 description 4
229930091371 Fructose Natural products 0.000 description 4
239000005715 Fructose Substances 0.000 description 4
RFSUNEUAIZKAJO-ARQDHWQXSA-N Fructose Chemical compound OC[C@H]1O[C@](O)(CO)[C@@H](O)[C@@H]1O RFSUNEUAIZKAJO-ARQDHWQXSA-N 0.000 description 4
241000223221 Fusarium oxysporum Species 0.000 description 4
241000567178 Fusarium venenatum Species 0.000 description 4
241001480714 Humicola insolens Species 0.000 description 4
MHAJPDPJQMAIIY-UHFFFAOYSA-N Hydrogen peroxide Chemical compound OO MHAJPDPJQMAIIY-UHFFFAOYSA-N 0.000 description 4
108010029541 Laccase Proteins 0.000 description 4
108090001060 Lipase Proteins 0.000 description 4
102000004882 Lipase Human genes 0.000 description 4
239000004367 Lipase Substances 0.000 description 4
101710117655 Maltogenic alpha-amylase Proteins 0.000 description 4
102000035195 Peptidases Human genes 0.000 description 4
108091005804 Peptidases Proteins 0.000 description 4
241000235403 Rhizomucor miehei Species 0.000 description 4
CDBYLPFSWZWCQE-UHFFFAOYSA-L Sodium Carbonate Chemical compound [Na+].[Na+].[O-]C([O-])=O CDBYLPFSWZWCQE-UHFFFAOYSA-L 0.000 description 4
FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 description 4
239000004098 Tetracycline Substances 0.000 description 4
241000223259 Trichoderma Species 0.000 description 4
108700015934 Triose-phosphate isomerases Proteins 0.000 description 4
240000008042 Zea mays Species 0.000 description 4
235000002017 Zea mays subsp mays Nutrition 0.000 description 4
239000008351 acetate buffer Substances 0.000 description 4
WQZGKKKJIJFFOK-DVKNGEFBSA-N alpha-D-glucose Chemical compound OC[C@H]1O[C@H](O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-DVKNGEFBSA-N 0.000 description 4
230000000721 bacterilogical effect Effects 0.000 description 4
108010051210 beta-Fructofuranosidase Proteins 0.000 description 4
108010047754 beta-Glucosidase Proteins 0.000 description 4
102000006995 beta-Glucosidase Human genes 0.000 description 4
229910052799 carbon Inorganic materials 0.000 description 4
229940106157 cellulase Drugs 0.000 description 4
230000021615 conjugation Effects 0.000 description 4
239000000499 gel Substances 0.000 description 4
108010061330 glucan 1,4-alpha-maltohydrolase Proteins 0.000 description 4
235000013922 glutamic acid Nutrition 0.000 description 4
239000004220 glutamic acid Substances 0.000 description 4
238000002744 homologous recombination Methods 0.000 description 4
230000006801 homologous recombination Effects 0.000 description 4
229940088597 hormone Drugs 0.000 description 4
239000005556 hormone Substances 0.000 description 4
239000001573 invertase Substances 0.000 description 4
235000011073 invertase Nutrition 0.000 description 4
238000002955 isolation Methods 0.000 description 4
239000004310 lactic acid Substances 0.000 description 4
235000014655 lactic acid Nutrition 0.000 description 4
235000019421 lipase Nutrition 0.000 description 4
239000007788 liquid Substances 0.000 description 4
230000007935 neutral effect Effects 0.000 description 4
235000015097 nutrients Nutrition 0.000 description 4
238000000746 purification Methods 0.000 description 4
230000035484 reaction time Effects 0.000 description 4
230000008929 regeneration Effects 0.000 description 4
238000011069 regeneration method Methods 0.000 description 4
238000012216 screening Methods 0.000 description 4
239000007787 solid Substances 0.000 description 4
229960002180 tetracycline Drugs 0.000 description 4
229930101283 tetracycline Natural products 0.000 description 4
235000019364 tetracycline Nutrition 0.000 description 4
150000003522 tetracyclines Chemical class 0.000 description 4
230000001131 transforming effect Effects 0.000 description 4
238000013519 translation Methods 0.000 description 4
235000020985 whole grains Nutrition 0.000 description 4
MTCFGRXMJLQNBG-REOHCLBHSA-N (2S)-2-Amino-3-hydroxypropansäure Chemical compound OC[C@H](N)C(O)=O MTCFGRXMJLQNBG-REOHCLBHSA-N 0.000 description 3
JAHNSTQSQJOJLO-UHFFFAOYSA-N 2-(3-fluorophenyl)-1h-imidazole Chemical compound FC1=CC=CC(C=2NC=CN=2)=C1 JAHNSTQSQJOJLO-UHFFFAOYSA-N 0.000 description 3
108010011619 6-Phytase Proteins 0.000 description 3
QTBSBXVTEAMEQO-UHFFFAOYSA-M Acetate Chemical compound CC([O-])=O QTBSBXVTEAMEQO-UHFFFAOYSA-M 0.000 description 3
229920000936 Agarose Polymers 0.000 description 3
102000004400 Aminopeptidases Human genes 0.000 description 3
108090000915 Aminopeptidases Proteins 0.000 description 3
108010037870 Anthranilate Synthase Proteins 0.000 description 3
102000004580 Aspartic Acid Proteases Human genes 0.000 description 3
108010017640 Aspartic Acid Proteases Proteins 0.000 description 3
101000775727 Bacillus amyloliquefaciens Alpha-amylase Proteins 0.000 description 3
102100026189 Beta-galactosidase Human genes 0.000 description 3
OKTJSMMVPCPJKN-UHFFFAOYSA-N Carbon Chemical compound [C] OKTJSMMVPCPJKN-UHFFFAOYSA-N 0.000 description 3
108010006303 Carboxypeptidases Proteins 0.000 description 3
102000005367 Carboxypeptidases Human genes 0.000 description 3
102000016938 Catalase Human genes 0.000 description 3
108010053835 Catalase Proteins 0.000 description 3
102000030523 Catechol oxidase Human genes 0.000 description 3
108010031396 Catechol oxidase Proteins 0.000 description 3
108010022172 Chitinases Proteins 0.000 description 3
102000012286 Chitinases Human genes 0.000 description 3
FBPFZTCFMRRESA-FSIIMWSLSA-N D-Glucitol Natural products OC[C@H](O)[C@H](O)[C@@H](O)[C@H](O)CO FBPFZTCFMRRESA-FSIIMWSLSA-N 0.000 description 3
AUNGANRZJHBGPY-UHFFFAOYSA-N D-Lyxoflavin Natural products OCC(O)C(O)C(O)CN1C=2C=C(C)C(C)=CC=2N=C2C1=NC(=O)NC2=O AUNGANRZJHBGPY-UHFFFAOYSA-N 0.000 description 3
RGHNJXZEOKUKBD-UHFFFAOYSA-N D-gluconic acid Natural products OCC(O)C(O)C(O)C(O)C(O)=O RGHNJXZEOKUKBD-UHFFFAOYSA-N 0.000 description 3
108010053770 Deoxyribonucleases Proteins 0.000 description 3
102000016911 Deoxyribonucleases Human genes 0.000 description 3
IAZDPXIOMUYVGZ-UHFFFAOYSA-N Dimethylsulphoxide Chemical compound CS(C)=O IAZDPXIOMUYVGZ-UHFFFAOYSA-N 0.000 description 3
101710121765 Endo-1,4-beta-xylanase Proteins 0.000 description 3
108090000371 Esterases Proteins 0.000 description 3
241000146406 Fusarium heterosporum Species 0.000 description 3
102000048120 Galactokinases Human genes 0.000 description 3
108700023157 Galactokinases Proteins 0.000 description 3
PEDCQBHIVMGVHV-UHFFFAOYSA-N Glycerine Chemical compound OCC(O)CO PEDCQBHIVMGVHV-UHFFFAOYSA-N 0.000 description 3
240000005979 Hordeum vulgare Species 0.000 description 3
235000007340 Hordeum vulgare Nutrition 0.000 description 3
125000000570 L-alpha-aspartyl group Chemical group [H]OC(=O)C([H])([H])[C@]([H])(N([H])[H])C(*)=O 0.000 description 3
DCXYFEDJOCDNAF-REOHCLBHSA-N L-asparagine Chemical compound OC(=O)[C@@H](N)CC(N)=O DCXYFEDJOCDNAF-REOHCLBHSA-N 0.000 description 3
102100024295 Maltase-glucoamylase Human genes 0.000 description 3
108010054377 Mannosidases Proteins 0.000 description 3
102000001696 Mannosidases Human genes 0.000 description 3
241000226677 Myceliophthora Species 0.000 description 3
229930182555 Penicillin Natural products 0.000 description 3
JGSARLDLIJGVTE-MBNYWOFBSA-N Penicillin G Chemical compound N([C@H]1[C@H]2SC([C@@H](N2C1=O)C(O)=O)(C)C)C(=O)CC1=CC=CC=C1 JGSARLDLIJGVTE-MBNYWOFBSA-N 0.000 description 3
241000228143 Penicillium Species 0.000 description 3
239000001888 Peptone Substances 0.000 description 3
108010080698 Peptones Proteins 0.000 description 3
108010083644 Ribonucleases Proteins 0.000 description 3
102000006382 Ribonucleases Human genes 0.000 description 3
241000235070 Saccharomyces Species 0.000 description 3
240000006394 Sorghum bicolor Species 0.000 description 3
235000011684 Sorghum saccharatum Nutrition 0.000 description 3
241000194017 Streptococcus Species 0.000 description 3
241000187747 Streptomyces Species 0.000 description 3
229930006000 Sucrose Natural products 0.000 description 3
CZMRCDWAGMRECN-UGDNZRGBSA-N Sucrose Chemical compound O[C@H]1[C@H](O)[C@@H](CO)O[C@@]1(CO)O[C@@H]1[C@H](O)[C@@H](O)[C@H](O)[C@@H](CO)O1 CZMRCDWAGMRECN-UGDNZRGBSA-N 0.000 description 3
241000223258 Thermomyces lanuginosus Species 0.000 description 3
108060008539 Transglutaminase Proteins 0.000 description 3
102000005924 Triose-Phosphate Isomerase Human genes 0.000 description 3
IXKSXJFAGXLQOQ-XISFHERQSA-N WHWLQLKPGQPMY Chemical compound C([C@@H](C(=O)N[C@@H](CC=1C2=CC=CC=C2NC=1)C(=O)N[C@@H](CC(C)C)C(=O)N[C@@H](CCC(N)=O)C(=O)N[C@@H](CC(C)C)C(=O)N1CCC[C@H]1C(=O)NCC(=O)N[C@@H](CCC(N)=O)C(=O)N[C@@H](CC(O)=O)C(=O)N1CCC[C@H]1C(=O)N[C@@H](CCSC)C(=O)N[C@@H](CC=1C=CC(O)=CC=1)C(O)=O)NC(=O)[C@@H](N)CC=1C2=CC=CC=C2NC=1)C1=CNC=N1 IXKSXJFAGXLQOQ-XISFHERQSA-N 0.000 description 3
230000002378 acidificating effect Effects 0.000 description 3
150000001298 alcohols Chemical class 0.000 description 3
OENHQHLEOONYIE-UKMVMLAPSA-N all-trans beta-carotene Natural products CC=1CCCC(C)(C)C=1/C=C/C(/C)=C/C=C/C(/C)=C/C=C/C=C(C)C=CC=C(C)C=CC1=C(C)CCCC1(C)C OENHQHLEOONYIE-UKMVMLAPSA-N 0.000 description 3
102000005840 alpha-Galactosidase Human genes 0.000 description 3
108010030291 alpha-Galactosidase Proteins 0.000 description 3
108010028144 alpha-Glucosidases Proteins 0.000 description 3
229960000723 ampicillin Drugs 0.000 description 3
AVKUERGKIZMTKX-NJBDSQKTSA-N ampicillin Chemical compound C1([C@@H](N)C(=O)N[C@H]2[C@H]3SC([C@@H](N3C2=O)C(O)=O)(C)C)=CC=CC=C1 AVKUERGKIZMTKX-NJBDSQKTSA-N 0.000 description 3
230000003321 amplification Effects 0.000 description 3
239000003242 anti bacterial agent Substances 0.000 description 3
229940088710 antibiotic agent Drugs 0.000 description 3
238000003149 assay kit Methods 0.000 description 3
108010005774 beta-Galactosidase Proteins 0.000 description 3
239000011648 beta-carotene Substances 0.000 description 3
TUPZEYHYWIEDIH-WAIFQNFQSA-N beta-carotene Natural products CC(=C/C=C/C=C(C)/C=C/C=C(C)/C=C/C1=C(C)CCCC1(C)C)C=CC=C(/C)C=CC2=CCCCC2(C)C TUPZEYHYWIEDIH-WAIFQNFQSA-N 0.000 description 3
235000013734 beta-carotene Nutrition 0.000 description 3
229960002747 betacarotene Drugs 0.000 description 3
210000004899 c-terminal region Anatomy 0.000 description 3
108010089934 carbohydrase Proteins 0.000 description 3
230000015556 catabolic process Effects 0.000 description 3
238000012512 characterization method Methods 0.000 description 3
230000002759 chromosomal effect Effects 0.000 description 3
238000003776 cleavage reaction Methods 0.000 description 3
239000002361 compost Substances 0.000 description 3
150000001875 compounds Chemical class 0.000 description 3
108010005400 cutinase Proteins 0.000 description 3
235000013399 edible fruits Nutrition 0.000 description 3
241001233957 eudicotyledons Species 0.000 description 3
108010038658 exo-1,4-beta-D-xylosidase Proteins 0.000 description 3
230000006870 function Effects 0.000 description 3
239000007789 gas Substances 0.000 description 3
239000000174 gluconic acid Substances 0.000 description 3
229950006191 gluconic acid Drugs 0.000 description 3
235000012208 gluconic acid Nutrition 0.000 description 3
102000006602 glyceraldehyde-3-phosphate dehydrogenase Human genes 0.000 description 3
108020004445 glyceraldehyde-3-phosphate dehydrogenase Proteins 0.000 description 3
229910001385 heavy metal Inorganic materials 0.000 description 3
150000002576 ketones Chemical class 0.000 description 3
230000001404 mediated effect Effects 0.000 description 3
LVHBHZANLOWSRM-UHFFFAOYSA-N methylenebutanedioic acid Natural products OC(=O)CC(=C)C(O)=O LVHBHZANLOWSRM-UHFFFAOYSA-N 0.000 description 3
238000010369 molecular cloning Methods 0.000 description 3
238000002703 mutagenesis Methods 0.000 description 3
231100000350 mutagenesis Toxicity 0.000 description 3
238000003199 nucleic acid amplification method Methods 0.000 description 3
150000007524 organic acids Chemical class 0.000 description 3
235000005985 organic acids Nutrition 0.000 description 3
230000002351 pectolytic effect Effects 0.000 description 3
229940049954 penicillin Drugs 0.000 description 3
235000019319 peptone Nutrition 0.000 description 3
229940085127 phytase Drugs 0.000 description 3
238000012545 processing Methods 0.000 description 3
230000004044 response Effects 0.000 description 3
235000019192 riboflavin Nutrition 0.000 description 3
229960002477 riboflavin Drugs 0.000 description 3
239000002151 riboflavin Substances 0.000 description 3
230000007017 scission Effects 0.000 description 3
230000028327 secretion Effects 0.000 description 3
239000000600 sorbitol Substances 0.000 description 3
239000005720 sucrose Substances 0.000 description 3
230000002103 transcriptional effect Effects 0.000 description 3
102000003601 transglutaminase Human genes 0.000 description 3
229940088594 vitamin Drugs 0.000 description 3
229930003231 vitamin Natural products 0.000 description 3
235000013343 vitamin Nutrition 0.000 description 3
239000011782 vitamin Substances 0.000 description 3
239000007221 ypg medium Substances 0.000 description 3
OENHQHLEOONYIE-JLTXGRSLSA-N β-Carotene Chemical compound CC=1CCCC(C)(C)C=1\C=C\C(\C)=C\C=C\C(\C)=C\C=C\C=C(/C)\C=C\C=C(/C)\C=C\C1=C(C)CCCC1(C)C OENHQHLEOONYIE-JLTXGRSLSA-N 0.000 description 3
JLIDBLDQVAYHNE-YKALOCIXSA-N (+)-Abscisic acid Chemical compound OC(=O)/C=C(/C)\C=C\[C@@]1(O)C(C)=CC(=O)CC1(C)C JLIDBLDQVAYHNE-YKALOCIXSA-N 0.000 description 2
101150028074 2 gene Proteins 0.000 description 2
OSJPPGNTCRNQQC-UWTATZPHSA-N 3-phospho-D-glyceric acid Chemical compound OC(=O)[C@H](O)COP(O)(O)=O OSJPPGNTCRNQQC-UWTATZPHSA-N 0.000 description 2
RLFWWDJHLFCNIJ-UHFFFAOYSA-N 4-aminoantipyrine Chemical compound CN1C(C)=C(N)C(=O)N1C1=CC=CC=C1 RLFWWDJHLFCNIJ-UHFFFAOYSA-N 0.000 description 2
RZVAJINKPMORJF-UHFFFAOYSA-N Acetaminophen Chemical compound CC(=O)NC1=CC=C(O)C=C1 RZVAJINKPMORJF-UHFFFAOYSA-N 0.000 description 2
101710197633 Actin-1 Proteins 0.000 description 2
102000007698 Alcohol dehydrogenase Human genes 0.000 description 2
108010021809 Alcohol dehydrogenase Proteins 0.000 description 2
102100034044 All-trans-retinol dehydrogenase [NAD(+)] ADH1B Human genes 0.000 description 2
101710193111 All-trans-retinol dehydrogenase [NAD(+)] ADH4 Proteins 0.000 description 2
101100163849 Arabidopsis thaliana ARS1 gene Proteins 0.000 description 2
241001225321 Aspergillus fumigatus Species 0.000 description 2
241001480052 Aspergillus japonicus Species 0.000 description 2
241000122821 Aspergillus kawachii Species 0.000 description 2
101000690713 Aspergillus niger Alpha-glucosidase Proteins 0.000 description 2
241000222400 Athelia Species 0.000 description 2
241001328122 Bacillus clausii Species 0.000 description 2
108010029675 Bacillus licheniformis alpha-amylase Proteins 0.000 description 2
241000193388 Bacillus thuringiensis Species 0.000 description 2
108091005658 Basic proteases Proteins 0.000 description 2
241000219310 Beta vulgaris subsp. vulgaris Species 0.000 description 2
240000002791 Brassica napus Species 0.000 description 2
125000001433 C-terminal amino-acid group Chemical group 0.000 description 2
244000025254 Cannabis sativa Species 0.000 description 2
241000123346 Chrysosporium Species 0.000 description 2
102000053602 DNA Human genes 0.000 description 2
102000010911 Enzyme Precursors Human genes 0.000 description 2
108010062466 Enzyme Precursors Proteins 0.000 description 2
241000206602 Eukaryota Species 0.000 description 2
241000145614 Fusarium bactridioides Species 0.000 description 2
241000567163 Fusarium cerealis Species 0.000 description 2
241000223194 Fusarium culmorum Species 0.000 description 2
241000223195 Fusarium graminearum Species 0.000 description 2
241001112697 Fusarium reticulatum Species 0.000 description 2
108700028146 Genetic Enhancer Elements Proteins 0.000 description 2
101100080316 Geobacillus stearothermophilus nprT gene Proteins 0.000 description 2
108010050375 Glucose 1-Dehydrogenase Proteins 0.000 description 2
241000223198 Humicola Species 0.000 description 2
102000004157 Hydrolases Human genes 0.000 description 2
108090000604 Hydrolases Proteins 0.000 description 2
206010020649 Hyperkeratosis Diseases 0.000 description 2
108010028688 Isoamylase Proteins 0.000 description 2
102000004195 Isomerases Human genes 0.000 description 2
108090000769 Isomerases Proteins 0.000 description 2
102100027612 Kallikrein-11 Human genes 0.000 description 2
QNAYBMKLOCPYGJ-REOHCLBHSA-N L-alanine Chemical compound C[C@H](N)C(O)=O QNAYBMKLOCPYGJ-REOHCLBHSA-N 0.000 description 2
125000002842 L-seryl group Chemical group O=C([*])[C@](N([H])[H])([H])C([H])([H])O[H] 0.000 description 2
AYFVYJQAPQTCCC-GBXIJSLDSA-N L-threonine Chemical compound C[C@@H](O)[C@H](N)C(O)=O AYFVYJQAPQTCCC-GBXIJSLDSA-N 0.000 description 2
108090000364 Ligases Proteins 0.000 description 2
102000003960 Ligases Human genes 0.000 description 2
102000004317 Lyases Human genes 0.000 description 2
108090000856 Lyases Proteins 0.000 description 2
CSNNHWWHGAXBCP-UHFFFAOYSA-L Magnesium sulfate Chemical compound [Mg+2].[O-][S+2]([O-])([O-])[O-] CSNNHWWHGAXBCP-UHFFFAOYSA-L 0.000 description 2
240000003183 Manihot esculenta Species 0.000 description 2
235000016735 Manihot esculenta subsp esculenta Nutrition 0.000 description 2
102100037812 Medium-wave-sensitive opsin 1 Human genes 0.000 description 2
108090000157 Metallothionein Proteins 0.000 description 2
244000061176 Nicotiana tabacum Species 0.000 description 2
235000002637 Nicotiana tabacum Nutrition 0.000 description 2
241000233654 Oomycetes Species 0.000 description 2
ISWSIDIOOBJBQZ-UHFFFAOYSA-N Phenol Chemical compound OC1=CC=CC=C1 ISWSIDIOOBJBQZ-UHFFFAOYSA-N 0.000 description 2
241000222395 Phlebia Species 0.000 description 2
241000235648 Pichia Species 0.000 description 2
240000004713 Pisum sativum Species 0.000 description 2
241000589516 Pseudomonas Species 0.000 description 2
108020004511 Recombinant DNA Proteins 0.000 description 2
101100097319 Schizosaccharomyces pombe (strain 972 / ATCC 24843) ala1 gene Proteins 0.000 description 2
235000007238 Secale cereale Nutrition 0.000 description 2
244000082988 Secale cereale Species 0.000 description 2
241000187432 Streptomyces coelicolor Species 0.000 description 2
KDYFGRWQOYBRFD-UHFFFAOYSA-N Succinic acid Natural products OC(=O)CCC(O)=O KDYFGRWQOYBRFD-UHFFFAOYSA-N 0.000 description 2
235000021536 Sugar beet Nutrition 0.000 description 2
241000222354 Trametes Species 0.000 description 2
102000004357 Transferases Human genes 0.000 description 2
108090000992 Transferases Proteins 0.000 description 2
108700019146 Transgenes Proteins 0.000 description 2
241000378866 Trichoderma koningii Species 0.000 description 2
235000021307 Triticum Nutrition 0.000 description 2
244000098338 Triticum aestivum Species 0.000 description 2
101710152431 Trypsin-like protease Proteins 0.000 description 2
240000006677 Vicia faba Species 0.000 description 2
235000010749 Vicia faba Nutrition 0.000 description 2
241000700605 Viruses Species 0.000 description 2
235000005824 Zea mays ssp. parviglumis Nutrition 0.000 description 2
235000016383 Zea mays subsp huehuetenangensis Nutrition 0.000 description 2
238000000246 agarose gel electrophoresis Methods 0.000 description 2
235000004279 alanine Nutrition 0.000 description 2
230000004075 alteration Effects 0.000 description 2
101150078331 ama-1 gene Proteins 0.000 description 2
229940091771 aspergillus fumigatus Drugs 0.000 description 2
229940097012 bacillus thuringiensis Drugs 0.000 description 2
108010019077 beta-Amylase Proteins 0.000 description 2
230000003115 biocidal effect Effects 0.000 description 2
230000015572 biosynthetic process Effects 0.000 description 2
238000009835 boiling Methods 0.000 description 2
KDYFGRWQOYBRFD-NUQCWPJISA-N butanedioic acid Chemical compound O[14C](=O)CC[14C](O)=O KDYFGRWQOYBRFD-NUQCWPJISA-N 0.000 description 2
150000001720 carbohydrates Chemical class 0.000 description 2
235000014633 carbohydrates Nutrition 0.000 description 2
239000001913 cellulose Substances 0.000 description 2
229920002678 cellulose Polymers 0.000 description 2
239000007795 chemical reaction product Substances 0.000 description 2
238000000224 chemical solution deposition Methods 0.000 description 2
210000003763 chloroplast Anatomy 0.000 description 2
210000000349 chromosome Anatomy 0.000 description 2
235000005822 corn Nutrition 0.000 description 2
235000013365 dairy product Nutrition 0.000 description 2
230000000593 degrading effect Effects 0.000 description 2
238000001514 detection method Methods 0.000 description 2
239000008121 dextrose Substances 0.000 description 2
238000004821 distillation Methods 0.000 description 2
210000002257 embryonic structure Anatomy 0.000 description 2
230000002255 enzymatic effect Effects 0.000 description 2
239000003797 essential amino acid Substances 0.000 description 2
235000020776 essential amino acid Nutrition 0.000 description 2
108010000165 exo-1,3-alpha-glucanase Proteins 0.000 description 2
238000000605 extraction Methods 0.000 description 2
238000001914 filtration Methods 0.000 description 2
235000013305 food Nutrition 0.000 description 2
235000021433 fructose syrup Nutrition 0.000 description 2
108020001507 fusion proteins Proteins 0.000 description 2
102000037865 fusion proteins Human genes 0.000 description 2
230000012010 growth Effects 0.000 description 2
239000000413 hydrolysate Substances 0.000 description 2
230000002209 hydrophobic effect Effects 0.000 description 2
230000001965 increasing effect Effects 0.000 description 2
230000001939 inductive effect Effects 0.000 description 2
238000001802 infusion Methods 0.000 description 2
229960000448 lactic acid Drugs 0.000 description 2
238000007834 ligase chain reaction Methods 0.000 description 2
235000009973 maize Nutrition 0.000 description 2
239000011159 matrix material Substances 0.000 description 2
238000005259 measurement Methods 0.000 description 2
238000003801 milling Methods 0.000 description 2
229910052757 nitrogen Inorganic materials 0.000 description 2
230000037361 pathway Effects 0.000 description 2
230000004481 post-translational protein modification Effects 0.000 description 2
FGIUAXJPYTZDNR-UHFFFAOYSA-N potassium nitrate Chemical compound [K+].[O-][N+]([O-])=O FGIUAXJPYTZDNR-UHFFFAOYSA-N 0.000 description 2
239000008057 potassium phosphate buffer Substances 0.000 description 2
235000012015 potatoes Nutrition 0.000 description 2
238000002360 preparation method Methods 0.000 description 2
101150054232 pyrG gene Proteins 0.000 description 2
239000002994 raw material Substances 0.000 description 2
239000001054 red pigment Substances 0.000 description 2
238000011160 research Methods 0.000 description 2
230000002441 reversible effect Effects 0.000 description 2
238000012552 review Methods 0.000 description 2
230000003248 secreting effect Effects 0.000 description 2
238000000926 separation method Methods 0.000 description 2
229910000029 sodium carbonate Inorganic materials 0.000 description 2
239000011780 sodium chloride Substances 0.000 description 2
238000002415 sodium dodecyl sulfate polyacrylamide gel electrophoresis Methods 0.000 description 2
VWDWKYIASSYTQR-UHFFFAOYSA-N sodium nitrate Chemical compound [Na+].[O-][N+]([O-])=O VWDWKYIASSYTQR-UHFFFAOYSA-N 0.000 description 2
239000002689 soil Substances 0.000 description 2
239000003381 stabilizer Substances 0.000 description 2
239000012089 stop solution Substances 0.000 description 2
150000008163 sugars Chemical class 0.000 description 2
230000008961 swelling Effects 0.000 description 2
229910021654 trace metal Inorganic materials 0.000 description 2
238000010361 transduction Methods 0.000 description 2
230000026683 transduction Effects 0.000 description 2
238000012546 transfer Methods 0.000 description 2
238000011426 transformation method Methods 0.000 description 2
239000012137 tryptone Substances 0.000 description 2
230000009105 vegetative growth Effects 0.000 description 2
DNIAPMSPPWPWGF-VKHMYHEASA-N (+)-propylene glycol Chemical compound C[C@H](O)CO DNIAPMSPPWPWGF-VKHMYHEASA-N 0.000 description 1
DIGQNXIGRZPYDK-WKSCXVIASA-N (2R)-6-amino-2-[[2-[[(2S)-2-[[2-[[(2R)-2-[[(2S)-2-[[(2R,3S)-2-[[2-[[(2S)-2-[[2-[[(2S)-2-[[(2S)-2-[[(2R)-2-[[(2S,3S)-2-[[(2R)-2-[[(2S)-2-[[(2S)-2-[[(2S)-2-[[2-[[(2S)-2-[[(2R)-2-[[2-[[2-[[2-[(2-amino-1-hydroxyethylidene)amino]-3-carboxy-1-hydroxypropylidene]amino]-1-hydroxy-3-sulfanylpropylidene]amino]-1-hydroxyethylidene]amino]-1-hydroxy-3-sulfanylpropylidene]amino]-1,3-dihydroxypropylidene]amino]-1-hydroxyethylidene]amino]-1-hydroxypropylidene]amino]-1,3-dihydroxypropylidene]amino]-1,3-dihydroxypropylidene]amino]-1-hydroxy-3-sulfanylpropylidene]amino]-1,3-dihydroxybutylidene]amino]-1-hydroxy-3-sulfanylpropylidene]amino]-1-hydroxypropylidene]amino]-1,3-dihydroxypropylidene]amino]-1-hydroxyethylidene]amino]-1,5-dihydroxy-5-iminopentylidene]amino]-1-hydroxy-3-sulfanylpropylidene]amino]-1,3-dihydroxybutylidene]amino]-1-hydroxy-3-sulfanylpropylidene]amino]-1,3-dihydroxypropylidene]amino]-1-hydroxyethylidene]amino]-1-hydroxy-3-sulfanylpropylidene]amino]-1-hydroxyethylidene]amino]hexanoic acid Chemical compound C[C@@H]([C@@H](C(=N[C@@H](CS)C(=N[C@@H](C)C(=N[C@@H](CO)C(=NCC(=N[C@@H](CCC(=N)O)C(=NC(CS)C(=N[C@H]([C@H](C)O)C(=N[C@H](CS)C(=N[C@H](CO)C(=NCC(=N[C@H](CS)C(=NCC(=N[C@H](CCCCN)C(=O)O)O)O)O)O)O)O)O)O)O)O)O)O)O)N=C([C@H](CS)N=C([C@H](CO)N=C([C@H](CO)N=C([C@H](C)N=C(CN=C([C@H](CO)N=C([C@H](CS)N=C(CN=C(C(CS)N=C(C(CC(=O)O)N=C(CN)O)O)O)O)O)O)O)O)O)O)O)O DIGQNXIGRZPYDK-WKSCXVIASA-N 0.000 description 1
OCUSNPIJIZCRSZ-ZTZWCFDHSA-N (2s)-2-amino-3-methylbutanoic acid;(2s)-2-amino-4-methylpentanoic acid;(2s,3s)-2-amino-3-methylpentanoic acid Chemical compound CC(C)[C@H](N)C(O)=O.CC[C@H](C)[C@H](N)C(O)=O.CC(C)C[C@H](N)C(O)=O OCUSNPIJIZCRSZ-ZTZWCFDHSA-N 0.000 description 1
FQVLRGLGWNWPSS-BXBUPLCLSA-N (4r,7s,10s,13s,16r)-16-acetamido-13-(1h-imidazol-5-ylmethyl)-10-methyl-6,9,12,15-tetraoxo-7-propan-2-yl-1,2-dithia-5,8,11,14-tetrazacycloheptadecane-4-carboxamide Chemical compound N1C(=O)[C@@H](NC(C)=O)CSSC[C@@H](C(N)=O)NC(=O)[C@H](C(C)C)NC(=O)[C@H](C)NC(=O)[C@@H]1CC1=CN=CN1 FQVLRGLGWNWPSS-BXBUPLCLSA-N 0.000 description 1
108091032973 (ribonucleotides)n+m Proteins 0.000 description 1
YPFDHNVEDLHUCE-UHFFFAOYSA-N 1,3-propanediol Substances OCCCO YPFDHNVEDLHUCE-UHFFFAOYSA-N 0.000 description 1
RXMWXENJQAINCC-DMTCNVIQSA-N 2,5-didehydro-D-gluconic acid Chemical compound OCC(=O)[C@@H](O)[C@H](O)C(=O)C(O)=O RXMWXENJQAINCC-DMTCNVIQSA-N 0.000 description 1
RXMWXENJQAINCC-UHFFFAOYSA-N 2,5-diketo-D-gluconic acid Natural products OCC(=O)C(O)C(O)C(=O)C(O)=O RXMWXENJQAINCC-UHFFFAOYSA-N 0.000 description 1
JKMHFZQWWAIEOD-UHFFFAOYSA-N 2-[4-(2-hydroxyethyl)piperazin-1-yl]ethanesulfonic acid Chemical compound OCC[NH+]1CCN(CCS([O-])(=O)=O)CC1 JKMHFZQWWAIEOD-UHFFFAOYSA-N 0.000 description 1
OSBLTNPMIGYQGY-UHFFFAOYSA-N 2-amino-2-(hydroxymethyl)propane-1,3-diol;2-[2-[bis(carboxymethyl)amino]ethyl-(carboxymethyl)amino]acetic acid;boric acid Chemical compound OB(O)O.OCC(N)(CO)CO.OC(=O)CN(CC(O)=O)CCN(CC(O)=O)CC(O)=O OSBLTNPMIGYQGY-UHFFFAOYSA-N 0.000 description 1
HOSWPDPVFBCLSY-UHFFFAOYSA-N 2-azaniumyl-4-oxobutanoate Chemical compound OC(=O)C(N)CC=O HOSWPDPVFBCLSY-UHFFFAOYSA-N 0.000 description 1
INEWUCPYEUEQTN-UHFFFAOYSA-N 3-(cyclohexylamino)-2-hydroxy-1-propanesulfonic acid Chemical compound OS(=O)(=O)CC(O)CNC1CCCCC1 INEWUCPYEUEQTN-UHFFFAOYSA-N 0.000 description 1
BTJIUGUIPKRLHP-UHFFFAOYSA-N 4-nitrophenol Chemical compound OC1=CC=C([N+]([O-])=O)C=C1 BTJIUGUIPKRLHP-UHFFFAOYSA-N 0.000 description 1
101710163881 5,6-dihydroxyindole-2-carboxylic acid oxidase Proteins 0.000 description 1
ZCYVEMRRCGMTRW-UHFFFAOYSA-N 7553-56-2 Chemical compound [I] ZCYVEMRRCGMTRW-UHFFFAOYSA-N 0.000 description 1
241001019659 Acremonium <Plectosphaerellaceae> Species 0.000 description 1
229930024421 Adenine Natural products 0.000 description 1
GFFGJBXGBJISGV-UHFFFAOYSA-N Adenine Chemical compound NC1=NC=NC2=C1N=CN2 GFFGJBXGBJISGV-UHFFFAOYSA-N 0.000 description 1
241000589158 Agrobacterium Species 0.000 description 1
241000589155 Agrobacterium tumefaciens Species 0.000 description 1
241000743339 Agrostis Species 0.000 description 1
108010088751 Albumins Proteins 0.000 description 1
102000009027 Albumins Human genes 0.000 description 1
102100034035 Alcohol dehydrogenase 1A Human genes 0.000 description 1
229920000945 Amylopectin Polymers 0.000 description 1
229920000856 Amylose Polymers 0.000 description 1
241000534414 Anotopterus nikparini Species 0.000 description 1
241000219195 Arabidopsis thaliana Species 0.000 description 1
239000004475 Arginine Substances 0.000 description 1
241000235349 Ascomycota Species 0.000 description 1
DCXYFEDJOCDNAF-UHFFFAOYSA-N Asparagine Natural products OC(=O)C(N)CC(N)=O DCXYFEDJOCDNAF-UHFFFAOYSA-N 0.000 description 1
241000228215 Aspergillus aculeatus Species 0.000 description 1
241001513093 Aspergillus awamori Species 0.000 description 1
101000961203 Aspergillus awamori Glucoamylase Proteins 0.000 description 1
241000892910 Aspergillus foetidus Species 0.000 description 1
101000924385 Aspergillus niger Acid alpha-amylase Proteins 0.000 description 1
101000756530 Aspergillus niger Endo-1,4-beta-xylanase B Proteins 0.000 description 1
101900127796 Aspergillus oryzae Glucoamylase Proteins 0.000 description 1
101900318521 Aspergillus oryzae Triosephosphate isomerase Proteins 0.000 description 1
241001530056 Athelia rolfsii Species 0.000 description 1
241000223651 Aureobasidium Species 0.000 description 1
235000007319 Avena orientalis Nutrition 0.000 description 1
244000075850 Avena orientalis Species 0.000 description 1
108090001008 Avidin Proteins 0.000 description 1
108090000145 Bacillolysin Proteins 0.000 description 1
241000193744 Bacillus amyloliquefaciens Species 0.000 description 1
241000193752 Bacillus circulans Species 0.000 description 1
241000193749 Bacillus coagulans Species 0.000 description 1
241000193422 Bacillus lentus Species 0.000 description 1
101000695691 Bacillus licheniformis Beta-lactamase Proteins 0.000 description 1
241000194107 Bacillus megaterium Species 0.000 description 1
241000194110 Bacillus sp. (in: Bacteria) Species 0.000 description 1
108010045681 Bacillus stearothermophilus neutral protease Proteins 0.000 description 1
241000221198 Basidiomycota Species 0.000 description 1
102100030981 Beta-alanine-activating enzyme Human genes 0.000 description 1
241000222478 Bjerkandera adusta Species 0.000 description 1
235000011293 Brassica napus Nutrition 0.000 description 1
235000011299 Brassica oleracea var botrytis Nutrition 0.000 description 1
240000003259 Brassica oleracea var. botrytis Species 0.000 description 1
235000004977 Brassica sinapistrum Nutrition 0.000 description 1
241000219193 Brassicaceae Species 0.000 description 1
241000193764 Brevibacillus brevis Species 0.000 description 1
239000008000 CHES buffer Substances 0.000 description 1
101100327917 Caenorhabditis elegans chup-1 gene Proteins 0.000 description 1
101100520142 Caenorhabditis elegans pin-2 gene Proteins 0.000 description 1
UXVMQQNJUSDDNG-UHFFFAOYSA-L Calcium chloride Chemical compound [Cl-].[Cl-].[Ca+2] UXVMQQNJUSDDNG-UHFFFAOYSA-L 0.000 description 1
208000003643 Callosities Diseases 0.000 description 1
241000589876 Campylobacter Species 0.000 description 1
241000222120 Candida <Saccharomycetales> Species 0.000 description 1
102000007132 Carboxyl and Carbamoyl Transferases Human genes 0.000 description 1
108010072957 Carboxyl and Carbamoyl Transferases Proteins 0.000 description 1
108010084185 Cellulases Proteins 0.000 description 1
102000005575 Cellulases Human genes 0.000 description 1
102100037633 Centrin-3 Human genes 0.000 description 1
241000146399 Ceriporiopsis Species 0.000 description 1
241001466517 Ceriporiopsis aneirina Species 0.000 description 1
241001277875 Ceriporiopsis rivulosa Species 0.000 description 1
229920002101 Chitin Polymers 0.000 description 1
229920001661 Chitosan Polymers 0.000 description 1
241000701248 Chlorella virus Species 0.000 description 1
241000985909 Chrysosporium keratinophilum Species 0.000 description 1
241001674013 Chrysosporium lucknowense Species 0.000 description 1
241001556045 Chrysosporium merdarium Species 0.000 description 1
241000080524 Chrysosporium queenslandicum Species 0.000 description 1
241001674001 Chrysosporium tropicum Species 0.000 description 1
241000355696 Chrysosporium zonatum Species 0.000 description 1
241000233652 Chytridiomycota Species 0.000 description 1
108020004638 Circular DNA Proteins 0.000 description 1
241000193403 Clostridium Species 0.000 description 1
108020004705 Codon Proteins 0.000 description 1
108700010070 Codon Usage Proteins 0.000 description 1
241000222511 Coprinus Species 0.000 description 1
244000251987 Coprinus macrorhizus Species 0.000 description 1
235000001673 Coprinus macrorhizus Nutrition 0.000 description 1
241000222356 Coriolus Species 0.000 description 1
241001337994 Cryptococcus <scale insect> Species 0.000 description 1
241001559589 Cullen Species 0.000 description 1
102000018832 Cytochromes Human genes 0.000 description 1
108010052832 Cytochromes Proteins 0.000 description 1
RGHNJXZEOKUKBD-SQOUGZDYSA-M D-gluconate Chemical compound OC[C@@H](O)[C@@H](O)[C@H](O)[C@@H](O)C([O-])=O RGHNJXZEOKUKBD-SQOUGZDYSA-M 0.000 description 1
230000004544 DNA amplification Effects 0.000 description 1
238000007400 DNA extraction Methods 0.000 description 1
239000003155 DNA primer Substances 0.000 description 1
239000003298 DNA probe Substances 0.000 description 1
238000001712 DNA sequencing Methods 0.000 description 1
102000016928 DNA-directed DNA polymerase Human genes 0.000 description 1
108010014303 DNA-directed DNA polymerase Proteins 0.000 description 1
101100342470 Dictyostelium discoideum pkbA gene Proteins 0.000 description 1
108090000204 Dipeptidase 1 Proteins 0.000 description 1
KCXVZYZYPLLWCC-UHFFFAOYSA-N EDTA Chemical compound OC(=O)CN(CC(O)=O)CCN(CC(O)=O)CC(O)=O KCXVZYZYPLLWCC-UHFFFAOYSA-N 0.000 description 1
101710132690 Endo-1,4-beta-xylanase A Proteins 0.000 description 1
241000194033 Enterococcus Species 0.000 description 1
101100385973 Escherichia coli (strain K12) cycA gene Proteins 0.000 description 1
VGGSQFUCUMXWEO-UHFFFAOYSA-N Ethene Chemical compound C=C VGGSQFUCUMXWEO-UHFFFAOYSA-N 0.000 description 1
239000005977 Ethylene Substances 0.000 description 1
LLQPHQFNMLZJMP-UHFFFAOYSA-N Fentrazamide Chemical compound N1=NN(C=2C(=CC=CC=2)Cl)C(=O)N1C(=O)N(CC)C1CCCCC1 LLQPHQFNMLZJMP-UHFFFAOYSA-N 0.000 description 1
241000234642 Festuca Species 0.000 description 1
241000192125 Firmicutes Species 0.000 description 1
241000589565 Flavobacterium Species 0.000 description 1
241001014439 Fusarium sarcochroum Species 0.000 description 1
241000223192 Fusarium sporotrichioides Species 0.000 description 1
241001465753 Fusarium torulosum Species 0.000 description 1
241000605909 Fusobacterium Species 0.000 description 1
101150108358 GLAA gene Proteins 0.000 description 1
241000146398 Gelatoporia subvermispora Species 0.000 description 1
206010064571 Gene mutation Diseases 0.000 description 1
108700007698 Genetic Terminator Regions Proteins 0.000 description 1
241000626621 Geobacillus Species 0.000 description 1
101100001650 Geobacillus stearothermophilus amyM gene Proteins 0.000 description 1
101100369308 Geobacillus stearothermophilus nprS gene Proteins 0.000 description 1
101000892220 Geobacillus thermodenitrificans (strain NG80-2) Long-chain-alcohol dehydrogenase 1 Proteins 0.000 description 1
239000005980 Gibberellic acid Substances 0.000 description 1
108010044091 Globulins Proteins 0.000 description 1
102000006395 Globulins Human genes 0.000 description 1
229920001503 Glucan Polymers 0.000 description 1
102000004366 Glucosidases Human genes 0.000 description 1
108010056771 Glucosidases Proteins 0.000 description 1
108010068370 Glutens Proteins 0.000 description 1
239000004471 Glycine Substances 0.000 description 1
244000068988 Glycine max Species 0.000 description 1
235000010469 Glycine max Nutrition 0.000 description 1
239000007995 HEPES buffer Substances 0.000 description 1
101150009006 HIS3 gene Proteins 0.000 description 1
101100295959 Halobacterium salinarum (strain ATCC 700922 / JCM 11081 / NRC-1) arcB gene Proteins 0.000 description 1
101100246753 Halobacterium salinarum (strain ATCC 700922 / JCM 11081 / NRC-1) pyrF gene Proteins 0.000 description 1
241000589989 Helicobacter Species 0.000 description 1
241000238631 Hexapoda Species 0.000 description 1
101000780443 Homo sapiens Alcohol dehydrogenase 1A Proteins 0.000 description 1
101000773364 Homo sapiens Beta-alanine-activating enzyme Proteins 0.000 description 1
101000880522 Homo sapiens Centrin-3 Proteins 0.000 description 1
101000882901 Homo sapiens Claudin-2 Proteins 0.000 description 1
101001035458 Humicola insolens Endoglucanase-5 Proteins 0.000 description 1
VEXZGXHMUGYJMC-UHFFFAOYSA-N Hydrochloric acid Chemical compound Cl VEXZGXHMUGYJMC-UHFFFAOYSA-N 0.000 description 1
244000017020 Ipomoea batatas Species 0.000 description 1
235000002678 Ipomoea batatas Nutrition 0.000 description 1
239000007836 KH2PO4 Substances 0.000 description 1
241000235649 Kluyveromyces Species 0.000 description 1
ODKSFYDXXFIFQN-BYPYZUCNSA-P L-argininium(2+) Chemical compound NC(=[NH2+])NCCC[C@H]([NH3+])C(O)=O ODKSFYDXXFIFQN-BYPYZUCNSA-P 0.000 description 1
CKLJMWTZIZZHCS-REOHCLBHSA-N L-aspartic acid Chemical compound OC(=O)[C@@H](N)CC(O)=O CKLJMWTZIZZHCS-REOHCLBHSA-N 0.000 description 1
ZDXPYRJPNDTMRX-VKHMYHEASA-N L-glutamine Chemical compound OC(=O)[C@@H](N)CCC(N)=O ZDXPYRJPNDTMRX-VKHMYHEASA-N 0.000 description 1
HNDVDQJCIGZPNO-YFKPBYRVSA-N L-histidine Chemical compound OC(=O)[C@@H](N)CC1=CN=CN1 HNDVDQJCIGZPNO-YFKPBYRVSA-N 0.000 description 1
125000003440 L-leucyl group Chemical group O=C([*])[C@](N([H])[H])([H])C([H])([H])C(C([H])([H])[H])([H])C([H])([H])[H] 0.000 description 1
KDXKERNSBIXSRK-YFKPBYRVSA-N L-lysine Chemical compound NCCCC[C@H](N)C(O)=O KDXKERNSBIXSRK-YFKPBYRVSA-N 0.000 description 1
125000001176 L-lysyl group Chemical group [H]N([H])[C@]([H])(C(=O)[*])C([H])([H])C([H])([H])C([H])([H])C(N([H])[H])([H])[H] 0.000 description 1
FFEARJCKVFRZRR-BYPYZUCNSA-N L-methionine Chemical compound CSCC[C@H](N)C(O)=O FFEARJCKVFRZRR-BYPYZUCNSA-N 0.000 description 1
FBOZXECLQNJBKD-ZDUSSCGKSA-N L-methotrexate Chemical compound C=1N=C2N=C(N)N=C(N)C2=NC=1CN(C)C1=CC=C(C(=O)N[C@@H](CCC(O)=O)C(O)=O)C=C1 FBOZXECLQNJBKD-ZDUSSCGKSA-N 0.000 description 1
COLNVLDHVKWLRT-QMMMGPOBSA-N L-phenylalanine Chemical compound OC(=O)[C@@H](N)CC1=CC=CC=C1 COLNVLDHVKWLRT-QMMMGPOBSA-N 0.000 description 1
125000000769 L-threonyl group Chemical group [H]N([H])[C@]([H])(C(=O)[*])[C@](O[H])(C([H])([H])[H])[H] 0.000 description 1
QIVBCDIJIAJPQS-VIFPVBQESA-N L-tryptophane Chemical compound C1=CC=C2C(C[C@H](N)C(O)=O)=CNC2=C1 QIVBCDIJIAJPQS-VIFPVBQESA-N 0.000 description 1
OUYCCCASQSFEME-QMMMGPOBSA-N L-tyrosine Chemical compound OC(=O)[C@@H](N)CC1=CC=C(O)C=C1 OUYCCCASQSFEME-QMMMGPOBSA-N 0.000 description 1
125000003798 L-tyrosyl group Chemical group [H]N([H])[C@]([H])(C(=O)[*])C([H])([H])C1=C([H])C([H])=C(O[H])C([H])=C1[H] 0.000 description 1
125000003580 L-valyl group Chemical group [H]N([H])[C@]([H])(C(=O)[*])C(C([H])([H])[H])(C([H])([H])[H])[H] 0.000 description 1
241000235087 Lachancea kluyveri Species 0.000 description 1
241000186660 Lactobacillus Species 0.000 description 1
241000194036 Lactococcus Species 0.000 description 1
101710094902 Legumin Proteins 0.000 description 1
108010036940 Levansucrase Proteins 0.000 description 1
241000209082 Lolium Species 0.000 description 1
241000219745 Lupinus Species 0.000 description 1
235000007688 Lycopersicon esculentum Nutrition 0.000 description 1
KDXKERNSBIXSRK-UHFFFAOYSA-N Lysine Natural products NCCCCC(N)C(O)=O KDXKERNSBIXSRK-UHFFFAOYSA-N 0.000 description 1
239000004472 Lysine Substances 0.000 description 1
101150068888 MET3 gene Proteins 0.000 description 1
241001344133 Magnaporthe Species 0.000 description 1
229920002774 Maltodextrin Polymers 0.000 description 1
239000005913 Maltodextrin Substances 0.000 description 1
229920000057 Mannan Polymers 0.000 description 1
102000003792 Metallothionein Human genes 0.000 description 1
108060004795 Methyltransferase Proteins 0.000 description 1
241000235395 Mucor Species 0.000 description 1
MKWKNSIESPFAQN-UHFFFAOYSA-N N-cyclohexyl-2-aminoethanesulfonic acid Chemical compound OS(=O)(=O)CCNC1CCCCC1 MKWKNSIESPFAQN-UHFFFAOYSA-N 0.000 description 1
125000001429 N-terminal alpha-amino-acid group Chemical group 0.000 description 1
125000000729 N-terminal amino-acid group Chemical group 0.000 description 1
238000005481 NMR spectroscopy Methods 0.000 description 1
241000233892 Neocallimastix Species 0.000 description 1
229930193140 Neomycin Natural products 0.000 description 1
241000221960 Neurospora Species 0.000 description 1
241000221961 Neurospora crassa Species 0.000 description 1
101100022915 Neurospora crassa (strain ATCC 24698 / 74-OR23-1A / CBS 708.71 / DSM 1257 / FGSC 987) cys-11 gene Proteins 0.000 description 1
108091005507 Neutral proteases Proteins 0.000 description 1
102000035092 Neutral proteases Human genes 0.000 description 1
108090000913 Nitrate Reductases Proteins 0.000 description 1
239000000020 Nitrocellulose Substances 0.000 description 1
102000015636 Oligopeptides Human genes 0.000 description 1
108010038807 Oligopeptides Proteins 0.000 description 1
108700026244 Open Reading Frames Proteins 0.000 description 1
102100037214 Orotidine 5'-phosphate decarboxylase Human genes 0.000 description 1
108010055012 Orotidine-5'-phosphate decarboxylase Proteins 0.000 description 1
229910019142 PO4 Inorganic materials 0.000 description 1
241001236817 Paecilomyces <Clavicipitaceae> Species 0.000 description 1
241000194109 Paenibacillus lautus Species 0.000 description 1
102100026367 Pancreatic alpha-amylase Human genes 0.000 description 1
206010034133 Pathogen resistance Diseases 0.000 description 1
108010087702 Penicillinase Proteins 0.000 description 1
241000222385 Phanerochaete Species 0.000 description 1
241000222393 Phanerochaete chrysosporium Species 0.000 description 1
244000100170 Phaseolus lunatus Species 0.000 description 1
235000010617 Phaseolus lunatus Nutrition 0.000 description 1
235000010627 Phaseolus vulgaris Nutrition 0.000 description 1
244000046052 Phaseolus vulgaris Species 0.000 description 1
108091000080 Phosphotransferase Proteins 0.000 description 1
241000425347 Phyla <beetle> Species 0.000 description 1
241000364051 Pima Species 0.000 description 1
241000235379 Piromyces Species 0.000 description 1
235000010582 Pisum sativum Nutrition 0.000 description 1
235000015622 Pisum sativum var macrocarpon Nutrition 0.000 description 1
241000222350 Pleurotus Species 0.000 description 1
241000209048 Poa Species 0.000 description 1
241000209049 Poa pratensis Species 0.000 description 1
241000209504 Poaceae Species 0.000 description 1
241000276498 Pollachius virens Species 0.000 description 1
239000004365 Protease Substances 0.000 description 1
102000001253 Protein Kinase Human genes 0.000 description 1
108020004518 RNA Probes Proteins 0.000 description 1
239000003391 RNA probe Substances 0.000 description 1
102000018120 Recombinases Human genes 0.000 description 1
108010091086 Recombinases Proteins 0.000 description 1
241000235402 Rhizomucor Species 0.000 description 1
101000968489 Rhizomucor miehei Lipase Proteins 0.000 description 1
101100394989 Rhodopseudomonas palustris (strain ATCC BAA-98 / CGA009) hisI gene Proteins 0.000 description 1
101900084120 Saccharomyces cerevisiae Triosephosphate isomerase Proteins 0.000 description 1
235000001006 Saccharomyces cerevisiae var diastaticus Nutrition 0.000 description 1
244000206963 Saccharomyces cerevisiae var. diastaticus Species 0.000 description 1
241000204893 Saccharomyces douglasii Species 0.000 description 1
241001407717 Saccharomyces norbensis Species 0.000 description 1
241000235343 Saccharomycetales Species 0.000 description 1
240000000111 Saccharum officinarum Species 0.000 description 1
235000007201 Saccharum officinarum Nutrition 0.000 description 1
241000607142 Salmonella Species 0.000 description 1
241000222480 Schizophyllum Species 0.000 description 1
241000235346 Schizosaccharomyces Species 0.000 description 1
101100022918 Schizosaccharomyces pombe (strain 972 / ATCC 24843) sua1 gene Proteins 0.000 description 1
MTCFGRXMJLQNBG-UHFFFAOYSA-N Serine Natural products OCC(N)C(O)=O MTCFGRXMJLQNBG-UHFFFAOYSA-N 0.000 description 1
240000003768 Solanum lycopersicum Species 0.000 description 1
241000191940 Staphylococcus Species 0.000 description 1
108091081024 Start codon Proteins 0.000 description 1
241000264435 Streptococcus dysgalactiae subsp. equisimilis Species 0.000 description 1
241000194048 Streptococcus equi Species 0.000 description 1
101100309436 Streptococcus mutans serotype c (strain ATCC 700610 / UA159) ftf gene Proteins 0.000 description 1
241000193996 Streptococcus pyogenes Species 0.000 description 1
241000194054 Streptococcus uberis Species 0.000 description 1
241000958303 Streptomyces achromogenes Species 0.000 description 1
241001468227 Streptomyces avermitilis Species 0.000 description 1
101100370749 Streptomyces coelicolor (strain ATCC BAA-471 / A3(2) / M145) trpC1 gene Proteins 0.000 description 1
241000187392 Streptomyces griseus Species 0.000 description 1
101100242848 Streptomyces hygroscopicus bar gene Proteins 0.000 description 1
241000187398 Streptomyces lividans Species 0.000 description 1
108090000787 Subtilisin Proteins 0.000 description 1
QAOWNCQODCNURD-UHFFFAOYSA-L Sulfate Chemical compound [O-]S([O-])(=O)=O QAOWNCQODCNURD-UHFFFAOYSA-L 0.000 description 1
108700005078 Synthetic Genes Proteins 0.000 description 1
239000008051 TBE buffer Substances 0.000 description 1
244000152045 Themeda triandra Species 0.000 description 1
101100157012 Thermoanaerobacterium saccharolyticum (strain DSM 8691 / JW/SL-YS485) xynB gene Proteins 0.000 description 1
241000228178 Thermoascus Species 0.000 description 1
235000009430 Thespesia populnea Nutrition 0.000 description 1
241001494489 Thielavia Species 0.000 description 1
241001495429 Thielavia terrestris Species 0.000 description 1
AYFVYJQAPQTCCC-UHFFFAOYSA-N Threonine Natural products CC(O)C(N)C(O)=O AYFVYJQAPQTCCC-UHFFFAOYSA-N 0.000 description 1
239000004473 Threonine Substances 0.000 description 1
108010022394 Threonine synthase Proteins 0.000 description 1
241001149964 Tolypocladium Species 0.000 description 1
241000222357 Trametes hirsuta Species 0.000 description 1
241000222355 Trametes versicolor Species 0.000 description 1
241000223262 Trichoderma longibrachiatum Species 0.000 description 1
241000223261 Trichoderma viride Species 0.000 description 1
229920004890 Triton X-100 Polymers 0.000 description 1
239000013504 Triton X-100 Substances 0.000 description 1
QIVBCDIJIAJPQS-UHFFFAOYSA-N Tryptophan Natural products C1=CC=C2C(CC(N)C(O)=O)=CNC2=C1 QIVBCDIJIAJPQS-UHFFFAOYSA-N 0.000 description 1
101150050575 URA3 gene Proteins 0.000 description 1
108090000848 Ubiquitin Proteins 0.000 description 1
102000044159 Ubiquitin Human genes 0.000 description 1
241000202898 Ureaplasma Species 0.000 description 1
241000409279 Xerochrysium dermatitidis Species 0.000 description 1
108700040099 Xylose isomerases Proteins 0.000 description 1
241000235015 Yarrowia lipolytica Species 0.000 description 1
241000758405 Zoopagomycotina Species 0.000 description 1
JUGOREOARAHOCO-UHFFFAOYSA-M acetylcholine chloride Chemical compound [Cl-].CC(=O)OCC[N+](C)(C)C JUGOREOARAHOCO-UHFFFAOYSA-M 0.000 description 1
238000010306 acid treatment Methods 0.000 description 1
229960000643 adenine Drugs 0.000 description 1
108010045649 agarase Proteins 0.000 description 1
238000012867 alanine scanning Methods 0.000 description 1
235000015107 ale Nutrition 0.000 description 1
108010050181 aleurone Proteins 0.000 description 1
101150069003 amdS gene Proteins 0.000 description 1
150000001408 amides Chemical class 0.000 description 1
238000012870 ammonium sulfate precipitation Methods 0.000 description 1
238000004458 analytical method Methods 0.000 description 1
238000000137 annealing Methods 0.000 description 1
230000000433 anti-nutritional effect Effects 0.000 description 1
230000000890 antigenic effect Effects 0.000 description 1
101150009206 aprE gene Proteins 0.000 description 1
101150008194 argB gene Proteins 0.000 description 1
ODKSFYDXXFIFQN-UHFFFAOYSA-N arginine Natural products OC(=O)C(N)CCCNC(N)=N ODKSFYDXXFIFQN-UHFFFAOYSA-N 0.000 description 1
210000004507 artificial chromosome Anatomy 0.000 description 1
235000009582 asparagine Nutrition 0.000 description 1
229960001230 asparagine Drugs 0.000 description 1
235000003704 aspartic acid Nutrition 0.000 description 1
235000020054 awamori Nutrition 0.000 description 1
229940054340 bacillus coagulans Drugs 0.000 description 1
230000008901 benefit Effects 0.000 description 1
OQFSQFPPLPISGP-UHFFFAOYSA-N beta-carboxyaspartic acid Natural products OC(=O)C(N)C(C(O)=O)C(O)=O OQFSQFPPLPISGP-UHFFFAOYSA-N 0.000 description 1
GUBGYTABKSRVRQ-QUYVBRFLSA-N beta-maltose Chemical compound OC[C@H]1O[C@H](O[C@H]2[C@H](O)[C@@H](O)[C@H](O)O[C@@H]2CO)[C@H](O)[C@@H](O)[C@@H]1O GUBGYTABKSRVRQ-QUYVBRFLSA-N 0.000 description 1
235000013361 beverage Nutrition 0.000 description 1
239000003139 biocide Substances 0.000 description 1
230000009141 biological interaction Effects 0.000 description 1
OWMVSZAMULFTJU-UHFFFAOYSA-N bis-tris Chemical compound OCCN(CCO)C(CO)(CO)CO OWMVSZAMULFTJU-UHFFFAOYSA-N 0.000 description 1
238000009395 breeding Methods 0.000 description 1
230000001488 breeding effect Effects 0.000 description 1
238000013124 brewing process Methods 0.000 description 1
102220359125 c.48G>A Human genes 0.000 description 1
239000001110 calcium chloride Substances 0.000 description 1
235000011148 calcium chloride Nutrition 0.000 description 1
229910001628 calcium chloride Inorganic materials 0.000 description 1
125000003178 carboxy group Chemical group [H]OC(*)=O 0.000 description 1
230000034303 cell budding Effects 0.000 description 1
239000013592 cell lysate Substances 0.000 description 1
230000010307 cell transformation Effects 0.000 description 1
210000002421 cell wall Anatomy 0.000 description 1
230000001413 cellular effect Effects 0.000 description 1
235000010980 cellulose Nutrition 0.000 description 1
238000005119 centrifugation Methods 0.000 description 1
235000013351 cheese Nutrition 0.000 description 1
239000003795 chemical substances by application Substances 0.000 description 1
229960005091 chloramphenicol Drugs 0.000 description 1
WIIZWVCIJKGZOK-RKDXNWHRSA-N chloramphenicol Chemical compound ClC(Cl)C(=O)N[C@H](CO)[C@H](O)C1=CC=C([N+]([O-])=O)C=C1 WIIZWVCIJKGZOK-RKDXNWHRSA-N 0.000 description 1
238000011098 chromatofocusing Methods 0.000 description 1
238000004587 chromatography analysis Methods 0.000 description 1
239000003283 colorimetric indicator Substances 0.000 description 1
238000009833 condensation Methods 0.000 description 1
230000005494 condensation Effects 0.000 description 1
239000000470 constituent Substances 0.000 description 1
238000010276 construction Methods 0.000 description 1
238000007796 conventional method Methods 0.000 description 1
210000000805 cytoplasm Anatomy 0.000 description 1
SUYVUBYJARFZHO-RRKCRQDMSA-N dATP Chemical compound C1=NC=2C(N)=NC=NC=2N1[C@H]1C[C@H](O)[C@@H](COP(O)(=O)OP(O)(=O)OP(O)(O)=O)O1 SUYVUBYJARFZHO-RRKCRQDMSA-N 0.000 description 1
SUYVUBYJARFZHO-UHFFFAOYSA-N dATP Natural products C1=NC=2C(N)=NC=NC=2N1C1CC(O)C(COP(O)(=O)OP(O)(=O)OP(O)(O)=O)O1 SUYVUBYJARFZHO-UHFFFAOYSA-N 0.000 description 1
RGWHQCVHVJXOKC-SHYZEUOFSA-J dCTP(4-) Chemical compound O=C1N=C(N)C=CN1[C@@H]1O[C@H](COP([O-])(=O)OP([O-])(=O)OP([O-])([O-])=O)[C@@H](O)C1 RGWHQCVHVJXOKC-SHYZEUOFSA-J 0.000 description 1
HAAZLUGHYHWQIW-KVQBGUIXSA-N dGTP Chemical compound C1=NC=2C(=O)NC(N)=NC=2N1[C@H]1C[C@H](O)[C@@H](COP(O)(=O)OP(O)(=O)OP(O)(O)=O)O1 HAAZLUGHYHWQIW-KVQBGUIXSA-N 0.000 description 1
NHVNXKFIZYSCEB-XLPZGREQSA-N dTTP Chemical compound O=C1NC(=O)C(C)=CN1[C@@H]1O[C@H](COP(O)(=O)OP(O)(=O)OP(O)(O)=O)[C@@H](O)C1 NHVNXKFIZYSCEB-XLPZGREQSA-N 0.000 description 1
101150005799 dagA gene Proteins 0.000 description 1
238000006731 degradation reaction Methods 0.000 description 1
239000005547 deoxyribonucleotide Substances 0.000 description 1
125000002637 deoxyribonucleotide group Chemical group 0.000 description 1
238000013461 design Methods 0.000 description 1
FCRACOPGPMPSHN-UHFFFAOYSA-N desoxyabscisic acid Natural products OC(=O)C=C(C)C=CC1C(C)=CC(=O)CC1(C)C FCRACOPGPMPSHN-UHFFFAOYSA-N 0.000 description 1
238000000502 dialysis Methods 0.000 description 1
238000002050 diffraction method Methods 0.000 description 1
230000008034 disappearance Effects 0.000 description 1
NEKNNCABDXGBEN-UHFFFAOYSA-L disodium;4-(4-chloro-2-methylphenoxy)butanoate;4-(2,4-dichlorophenoxy)butanoate Chemical compound [Na+].[Na+].CC1=CC(Cl)=CC=C1OCCCC([O-])=O.[O-]C(=O)CCCOC1=CC=C(Cl)C=C1Cl NEKNNCABDXGBEN-UHFFFAOYSA-L 0.000 description 1
239000012153 distilled water Substances 0.000 description 1
230000035622 drinking Effects 0.000 description 1
238000007876 drug discovery Methods 0.000 description 1
238000002003 electron diffraction Methods 0.000 description 1
230000008030 elimination Effects 0.000 description 1
238000003379 elimination reaction Methods 0.000 description 1
108010091371 endoglucanase 1 Proteins 0.000 description 1
108010091384 endoglucanase 2 Proteins 0.000 description 1
108010092413 endoglucanase V Proteins 0.000 description 1
238000005516 engineering process Methods 0.000 description 1
238000006911 enzymatic reaction Methods 0.000 description 1
238000001952 enzyme assay Methods 0.000 description 1
210000002615 epidermis Anatomy 0.000 description 1
239000000262 estrogen Substances 0.000 description 1
125000001495 ethyl group Chemical group [H]C([H])([H])C([H])([H])* 0.000 description 1
210000003527 eukaryotic cell Anatomy 0.000 description 1
238000001704 evaporation Methods 0.000 description 1
230000008020 evaporation Effects 0.000 description 1
230000007717 exclusion Effects 0.000 description 1
238000002474 experimental method Methods 0.000 description 1
239000000284 extract Substances 0.000 description 1
235000021001 fermented dairy product Nutrition 0.000 description 1
239000000835 fiber Substances 0.000 description 1
239000004459 forage Substances 0.000 description 1
239000000446 fuel Substances 0.000 description 1
239000010437 gem Substances 0.000 description 1
IXORZMNAPKEEDV-UHFFFAOYSA-N gibberellic acid GA3 Natural products OC(=O)C1C2(C3)CC(=C)C3(O)CCC2C2(C=CC3O)C1C3(C)C(=O)O2 IXORZMNAPKEEDV-UHFFFAOYSA-N 0.000 description 1
IXORZMNAPKEEDV-OBDJNFEBSA-N gibberellin A3 Chemical compound C([C@@]1(O)C(=C)C[C@@]2(C1)[C@H]1C(O)=O)C[C@H]2[C@]2(C=C[C@@H]3O)[C@H]1[C@]3(C)C(=O)O2 IXORZMNAPKEEDV-OBDJNFEBSA-N 0.000 description 1
229940050410 gluconate Drugs 0.000 description 1
ZDXPYRJPNDTMRX-UHFFFAOYSA-N glutamine Natural products OC(=O)C(N)CCC(N)=O ZDXPYRJPNDTMRX-UHFFFAOYSA-N 0.000 description 1
230000013595 glycosylation Effects 0.000 description 1
238000006206 glycosylation reaction Methods 0.000 description 1
PCHJSUWPFVWCPO-UHFFFAOYSA-N gold Chemical compound [Au] PCHJSUWPFVWCPO-UHFFFAOYSA-N 0.000 description 1
229910052737 gold Inorganic materials 0.000 description 1
239000010931 gold Substances 0.000 description 1
HNDVDQJCIGZPNO-UHFFFAOYSA-N histidine Natural products OC(=O)C(N)CC1=CN=CN1 HNDVDQJCIGZPNO-UHFFFAOYSA-N 0.000 description 1
230000007062 hydrolysis Effects 0.000 description 1
238000006460 hydrolysis reaction Methods 0.000 description 1
230000003301 hydrolyzing effect Effects 0.000 description 1
108010002685 hygromycin-B kinase Proteins 0.000 description 1
238000001727 in vivo Methods 0.000 description 1
230000017730 intein-mediated protein splicing Effects 0.000 description 1
230000003834 intracellular effect Effects 0.000 description 1
229910052740 iodine Inorganic materials 0.000 description 1
239000011630 iodine Substances 0.000 description 1
238000005342 ion exchange Methods 0.000 description 1
230000002427 irreversible effect Effects 0.000 description 1
238000001155 isoelectric focusing Methods 0.000 description 1
229960000318 kanamycin Drugs 0.000 description 1
229930027917 kanamycin Natural products 0.000 description 1
SBUJHOSQTJFQJX-NOAMYHISSA-N kanamycin Chemical compound O[C@@H]1[C@@H](O)[C@H](O)[C@@H](CN)O[C@@H]1O[C@H]1[C@H](O)[C@@H](O[C@@H]2[C@@H]([C@@H](N)[C@H](O)[C@@H](CO)O2)O)[C@H](N)C[C@@H]1N SBUJHOSQTJFQJX-NOAMYHISSA-N 0.000 description 1
229930182823 kanamycin A Natural products 0.000 description 1
229940039696 lactobacillus Drugs 0.000 description 1
235000015095 lager Nutrition 0.000 description 1
239000010985 leather Substances 0.000 description 1
235000021374 legumes Nutrition 0.000 description 1
235000021440 light beer Nutrition 0.000 description 1
101150039489 lysZ gene Proteins 0.000 description 1
229910052943 magnesium sulfate Inorganic materials 0.000 description 1
235000019341 magnesium sulphate Nutrition 0.000 description 1
229940035034 maltodextrin Drugs 0.000 description 1
235000012054 meals Nutrition 0.000 description 1
239000012528 membrane Substances 0.000 description 1
230000002503 metabolic effect Effects 0.000 description 1
229930182817 methionine Natural products 0.000 description 1
125000001360 methionine group Chemical group N[C@@H](CCSC)C(=O)* 0.000 description 1
229960000485 methotrexate Drugs 0.000 description 1
230000000813 microbial effect Effects 0.000 description 1
230000002906 microbiologic effect Effects 0.000 description 1
238000000520 microinjection Methods 0.000 description 1
210000003470 mitochondria Anatomy 0.000 description 1
235000013379 molasses Nutrition 0.000 description 1
229910000402 monopotassium phosphate Inorganic materials 0.000 description 1
235000019796 monopotassium phosphate Nutrition 0.000 description 1
239000004570 mortar (masonry) Substances 0.000 description 1
229960004927 neomycin Drugs 0.000 description 1
101150095344 niaD gene Proteins 0.000 description 1
229930027945 nicotinamide-adenine dinucleotide Natural products 0.000 description 1
BOPGDPNILDQYTO-NNYOXOHSSA-N nicotinamide-adenine dinucleotide Chemical compound C1=CCC(C(=O)N)=CN1[C@H]1[C@H](O)[C@H](O)[C@@H](COP(O)(=O)OP(O)(=O)OC[C@@H]2[C@H]([C@@H](O)[C@@H](O2)N2C3=NC=NC(N)=C3N=C2)O)O1 BOPGDPNILDQYTO-NNYOXOHSSA-N 0.000 description 1
229920001220 nitrocellulos Polymers 0.000 description 1
101150105920 npr gene Proteins 0.000 description 1
230000031787 nutrient reservoir activity Effects 0.000 description 1
235000016709 nutrition Nutrition 0.000 description 1
229920001542 oligosaccharide Polymers 0.000 description 1
210000000056 organ Anatomy 0.000 description 1
108090000021 oryzin Proteins 0.000 description 1
230000003647 oxidation Effects 0.000 description 1
238000007254 oxidation reaction Methods 0.000 description 1
235000019629 palatability Nutrition 0.000 description 1
210000002824 peroxisome Anatomy 0.000 description 1
235000020030 perry Nutrition 0.000 description 1
238000002823 phage display Methods 0.000 description 1
JTJMJGYZQZDUJJ-UHFFFAOYSA-N phencyclidine Chemical compound C1CCCCN1C1(C=2C=CC=CC=2)CCCCC1 JTJMJGYZQZDUJJ-UHFFFAOYSA-N 0.000 description 1
COLNVLDHVKWLRT-UHFFFAOYSA-N phenylalanine Natural products OC(=O)C(N)CC1=CC=CC=C1 COLNVLDHVKWLRT-UHFFFAOYSA-N 0.000 description 1
NBIIXXVUZAFLBC-UHFFFAOYSA-K phosphate Chemical compound [O-]P([O-])([O-])=O NBIIXXVUZAFLBC-UHFFFAOYSA-K 0.000 description 1
239000010452 phosphate Substances 0.000 description 1
108010082527 phosphinothricin N-acetyltransferase Proteins 0.000 description 1
230000026731 phosphorylation Effects 0.000 description 1
238000006366 phosphorylation reaction Methods 0.000 description 1
102000020233 phosphotransferase Human genes 0.000 description 1
238000005222 photoaffinity labeling Methods 0.000 description 1
239000003375 plant hormone Substances 0.000 description 1
230000037039 plant physiology Effects 0.000 description 1
238000002264 polyacrylamide gel electrophoresis Methods 0.000 description 1
229920000166 polytrimethylene carbonate Polymers 0.000 description 1
235000020004 porter Nutrition 0.000 description 1
230000001124 posttranscriptional effect Effects 0.000 description 1
GNSKLFRGEWLPPA-UHFFFAOYSA-M potassium dihydrogen phosphate Chemical compound [K+].OP(O)([O-])=O GNSKLFRGEWLPPA-UHFFFAOYSA-M 0.000 description 1
235000010333 potassium nitrate Nutrition 0.000 description 1
238000011533 pre-incubation Methods 0.000 description 1
238000001556 precipitation Methods 0.000 description 1
239000002243 precursor Substances 0.000 description 1
125000002924 primary amino group Chemical group [H]N([H])* 0.000 description 1
210000001236 prokaryotic cell Anatomy 0.000 description 1
108060006613 prolamin Proteins 0.000 description 1
230000001902 propagating effect Effects 0.000 description 1
235000019419 proteases Nutrition 0.000 description 1
108060006633 protein kinase Proteins 0.000 description 1
238000001742 protein purification Methods 0.000 description 1
101150108007 prs gene Proteins 0.000 description 1
101150086435 prs1 gene Proteins 0.000 description 1
101150070305 prsA gene Proteins 0.000 description 1
239000011535 reaction buffer Substances 0.000 description 1
108020003175 receptors Proteins 0.000 description 1
102000005962 receptors Human genes 0.000 description 1
230000006798 recombination Effects 0.000 description 1
238000005215 recombination Methods 0.000 description 1
235000020071 rectified spirit Nutrition 0.000 description 1
230000022532 regulation of transcription, DNA-dependent Effects 0.000 description 1
230000003362 replicative effect Effects 0.000 description 1
238000010839 reverse transcription Methods 0.000 description 1
108020004418 ribosomal RNA Proteins 0.000 description 1
101150025220 sacB gene Proteins 0.000 description 1
150000003839 salts Chemical class 0.000 description 1
102000012498 secondary active transmembrane transporter activity proteins Human genes 0.000 description 1
108040003878 secondary active transmembrane transporter activity proteins Proteins 0.000 description 1
101150091813 shfl gene Proteins 0.000 description 1
238000002741 site-directed mutagenesis Methods 0.000 description 1
235000010344 sodium nitrate Nutrition 0.000 description 1
238000010563 solid-state fermentation Methods 0.000 description 1
229960000268 spectinomycin Drugs 0.000 description 1
UNFWWIHTNXNPBV-WXKVUWSESA-N spectinomycin Chemical compound O([C@@H]1[C@@H](NC)[C@@H](O)[C@H]([C@@H]([C@H]1O1)O)NC)[C@]2(O)[C@H]1O[C@H](C)CC2=O UNFWWIHTNXNPBV-WXKVUWSESA-N 0.000 description 1
238000001694 spray drying Methods 0.000 description 1
238000010561 standard procedure Methods 0.000 description 1
238000003860 storage Methods 0.000 description 1
235000015106 stout Nutrition 0.000 description 1
229940115922 streptococcus uberis Drugs 0.000 description 1
239000000725 suspension Substances 0.000 description 1
230000002194 synthesizing effect Effects 0.000 description 1
238000012360 testing method Methods 0.000 description 1
238000001890 transfection Methods 0.000 description 1
230000014616 translation Effects 0.000 description 1
238000011282 treatment Methods 0.000 description 1
101150016309 trpC gene Proteins 0.000 description 1
WFKWXMTUELFFGS-UHFFFAOYSA-N tungsten Chemical compound [W] WFKWXMTUELFFGS-UHFFFAOYSA-N 0.000 description 1
229910052721 tungsten Inorganic materials 0.000 description 1
239000010937 tungsten Substances 0.000 description 1
OUYCCCASQSFEME-UHFFFAOYSA-N tyrosine Natural products OC(=O)C(N)CC1=CC=C(O)C=C1 OUYCCCASQSFEME-UHFFFAOYSA-N 0.000 description 1
238000011144 upstream manufacturing Methods 0.000 description 1
210000003934 vacuole Anatomy 0.000 description 1
230000002792 vascular Effects 0.000 description 1
235000015112 vegetable and seed oil Nutrition 0.000 description 1
239000003643 water by type Substances 0.000 description 1
239000002023 wood Substances 0.000 description 1
101150110790 xylB gene Proteins 0.000 description 1
210000005253 yeast cell Anatomy 0.000 description 1
235000013618 yogurt Nutrition 0.000 description 1
NWONKYPBYAMBJT-UHFFFAOYSA-L zinc sulfate Chemical compound [Zn+2].[O-]S([O-])(=O)=O NWONKYPBYAMBJT-UHFFFAOYSA-L 0.000 description 1
229910000368 zinc sulfate Inorganic materials 0.000 description 1
239000011686 zinc sulphate Substances 0.000 description 1
235000009529 zinc sulphate Nutrition 0.000 description 1

Classifications

- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
- C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
- C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
- C12N9/2405—Glucanases
- C12N9/2408—Glucanases acting on alpha -1,4-glucosidic bonds
- C12N9/2411—Amylases
- C12N9/2428—Glucan 1,4-alpha-glucosidase (3.2.1.3), i.e. glucoamylase
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES, NOT OTHERWISE PROVIDED FOR; PREPARATION OR TREATMENT THEREOF
- A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
- A23L33/10—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives
- A23L33/17—Amino acids, peptides or proteins
- A23L33/18—Peptides; Protein hydrolysates
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12C—BEER; PREPARATION OF BEER BY FERMENTATION; PREPARATION OF MALT FOR MAKING BEER; PREPARATION OF HOPS FOR MAKING BEER
- C12C5/00—Other raw materials for the preparation of beer
- C12C5/004—Enzymes
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P19/00—Preparation of compounds containing saccharide radicals
- C12P19/02—Monosaccharides
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P19/00—Preparation of compounds containing saccharide radicals
- C12P19/14—Preparation of compounds containing saccharide radicals produced by the action of a carbohydrase (EC 3.2.x), e.g. by alpha-amylase, e.g. by cellulase, hemicellulase
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Y—ENZYMES
- C12Y302/00—Hydrolases acting on glycosyl compounds, i.e. glycosylases (3.2)
- C12Y302/01—Glycosidases, i.e. enzymes hydrolysing O- and S-glycosyl compounds (3.2.1)
- C12Y302/01003—Glucan 1,4-alpha-glucosidase (3.2.1.3), i.e. glucoamylase

Landscapes

Chemical & Material Sciences (AREA)
Life Sciences & Earth Sciences (AREA)
Organic Chemistry (AREA)
Engineering & Computer Science (AREA)
Health & Medical Sciences (AREA)
Zoology (AREA)
Wood Science & Technology (AREA)
Bioinformatics & Cheminformatics (AREA)
Genetics & Genomics (AREA)
Biochemistry (AREA)
General Health & Medical Sciences (AREA)
General Engineering & Computer Science (AREA)
Biotechnology (AREA)
Microbiology (AREA)
General Chemical & Material Sciences (AREA)
Molecular Biology (AREA)
Food Science & Technology (AREA)
Chemical Kinetics & Catalysis (AREA)
Mycology (AREA)
Proteomics, Peptides & Aminoacids (AREA)
Nutrition Science (AREA)
Polymers & Plastics (AREA)
Medicinal Chemistry (AREA)
Biomedical Technology (AREA)
Micro-Organisms Or Cultivation Processes Thereof (AREA)
Enzymes And Modification Thereof (AREA)

CA2846690A 2011-08-26 2012-08-24 Polypeptides ayant une activite glucoamylase et polynucleotides codant pour ces polypeptides Abandoned CA2846690A1 (fr)

Applications Claiming Priority (3)

Application Number	Priority Date	Filing Date	Title
CNPCT/CN2011/078999		2011-08-26
CN2011078999		2011-08-26
PCT/CN2012/080553 WO2013029496A1 (fr)	2011-08-26	2012-08-24	Polypeptides ayant une activité glucoamylase et polynucléotides codant pour ces polypeptides

Publications (1)

Publication Number	Publication Date
CA2846690A1 true CA2846690A1 (fr)	2013-03-07

Family

ID=47755316

Family Applications (1)

Application Number	Title	Priority Date	Filing Date
CA2846690A Abandoned CA2846690A1 (fr)	2011-08-26	2012-08-24	Polypeptides ayant une activite glucoamylase et polynucleotides codant pour ces polypeptides

Country Status (3)

Country	Link
EP (1)	EP2748188A4 (fr)
CA (1)	CA2846690A1 (fr)
WO (1)	WO2013029496A1 (fr)

Families Citing this family (14)

* Cited by examiner, † Cited by third party
Publication number	Priority date	Publication date	Assignee	Title
US10208297B2 (en)	2014-01-22	2019-02-19	Novozymes A/S	Polypeptides with lipase activity and polynucleotides encoding same for cleaning
EP3521434A1 (fr)	2014-03-12	2019-08-07	Novozymes A/S	Polypeptides à activité lipase et polynucléotides codant pour ceux-ci
WO2015158237A1 (fr)	2014-04-15	2015-10-22	Novozymes A/S	Polypeptides à activité lipase et polynucléotides codant pour ceux-ci
MX383142B (es)	2014-12-19	2025-03-13	Novozymes As	Composiciones que comprenden polipéptidos que tienen actividad xilanasa y polipéptidos que tienen actividad arabinofuranosidasa.
BR112017021489A2 (pt)	2015-05-27	2018-07-03	Novozymes A/S	polipeptídeo tendo atividade de celobio-hidrolase, composição, formulação de caldo inteiro ou composição de cultura de células, polinucleotídeo, construto de ácido nucleico ou vetor de expressão, célula hospedeira recombinante, métodos de produção do polipeptídeo.
MX387681B (es)	2015-07-02	2025-03-18	Novozymes As	Composiciones de pienso para animales y uso de las mismas.
US10479984B2 (en)	2015-09-22	2019-11-19	Novozymes A/S	Polypeptides having cellobiohydrolase activity and polynucleotides encoding same
US11965189B2 (en)	2016-03-24	2024-04-23	Novozymes A/S	Cellobiohydrolase variants and polynucleotides encoding same
CN109196098A (zh)	2016-05-24	2019-01-11	诺维信公司	包含具有半乳聚糖酶活性的多肽和具有β-半乳糖苷酶活性的多肽的组合物
US10986850B2 (en)	2016-12-21	2021-04-27	Novozymes A/S	Polypeptides having lysozyme activity, polynucleotides encoding same and uses and compositions thereof
WO2018224035A1 (fr)	2017-06-09	2018-12-13	Novozymes A/S	Polypeptide, utilisation et procédé d'hydrolyse de protéine
EP3673039B1 (fr) *	2017-09-29	2025-03-19	International N&H Denmark ApS	Production de moût de brasserie ayant des sucres fermentescibles accrus
JP2025501588A (ja) *	2021-12-24	2025-01-22	ダニスコ・ユーエス・インク	ハイブリッドグルコアミラーゼ及びその使用方法
EP4525615A2 (fr)	2022-05-14	2025-03-26	Novozymes A/S	Compositions et procédés de prévention, de traitement, de suppression et/ou d'élimination d'infestations et d'infections phytopathogènes

Family Cites Families (39)

* Cited by examiner, † Cited by third party
Publication number	Priority date	Publication date	Assignee	Title
DK135983D0 (da)	1983-03-25	1983-03-25	Novo Industri As	Maltogen amylaseenzymprodukt og fremgangsmade til dets fremstilling og anvendelse
DK122686D0 (da)	1986-03-17	1986-03-17	Novo Industri As	Fremstilling af proteiner
WO1989001969A1 (fr)	1987-09-04	1989-03-09	Novo-Nordisk A/S	Procede de production de proteines dans aspergillus et promoteurs destines a exprimer ce champignon
US5223409A (en)	1988-09-02	1993-06-29	Protein Engineering Corp.	Directed evolution of novel binding proteins
NZ237549A (en)	1990-03-23	1993-06-25	Gist Brocades Nv	Production of enhanced levels of enzymes in the seeds of transgenic plants and the use of these seeds
US6395966B1 (en)	1990-08-09	2002-05-28	Dekalb Genetics Corp.	Fertile transgenic maize plants containing a gene encoding the pat protein
IL99552A0 (en)	1990-09-28	1992-08-18	Ixsys Inc	Compositions containing procaryotic cells,a kit for the preparation of vectors useful for the coexpression of two or more dna sequences and methods for the use thereof
US5231017A (en)	1991-05-17	1993-07-27	Solvay Enzymes, Inc.	Process for producing ethanol
DE4343591A1 (de)	1993-12-21	1995-06-22	Evotec Biosystems Gmbh	Verfahren zum evolutiven Design und Synthese funktionaler Polymere auf der Basis von Formenelementen und Formencodes
US5605793A (en)	1994-02-17	1997-02-25	Affymax Technologies N.V.	Methods for in vitro recombination
FI964808A0 (fi)	1994-06-03	1996-12-02	Novo Nordisk Biotech Inc	Puhdistetut Myceliophthora lakkaasit ja niitä koodaavat nukleiinihapot
CN101659926A (zh)	1994-06-30	2010-03-03	诺沃奇梅兹有限公司	非毒性、非产毒性、非致病性镰孢属表达系统及所用启动子和终止子
AR000862A1 (es)	1995-02-03	1997-08-06	Novozymes As	Variantes de una ó-amilasa madre, un metodo para producir la misma, una estructura de adn y un vector de expresion, una celula transformada por dichaestructura de adn y vector, un aditivo para detergente, composicion detergente, una composicion para lavado de ropa y una composicion para la eliminacion del
KR100511499B1 (ko)	1995-02-03	2005-12-21	노보자임스 에이/에스	소정 특성을 가지는 알파-아밀라제 돌연변이체를 디자인하는 방법
US6093562A (en)	1996-02-05	2000-07-25	Novo Nordisk A/S	Amylase variants
ES2432519T3 (es)	1996-04-30	2013-12-04	Novozymes A/S	Mutantes de alfa-amilasa
US6187576B1 (en)	1997-10-13	2001-02-13	Novo Nordisk A/S	α-amylase mutants
CN1261567C (zh) *	1997-11-26	2006-06-28	诺维信公司	热稳定的葡糖淀粉酶
CN1292028B (zh)	1998-02-27	2013-08-14	诺维信公司	麦芽α淀粉酶变体
CN1197965C (zh)	1998-10-26	2005-04-20	诺维信公司	在丝状真菌细胞内构建和筛选目的dna文库
CN100510096C (zh)	1999-03-22	2009-07-08	诺沃奇梅兹有限公司	用于在真菌细胞中表达基因的启动子
WO2000060059A2 (fr)	1999-03-30	2000-10-12	NovozymesA/S	Variantes d'alpha amylase
EP2308980A3 (fr)	2000-08-01	2011-04-27	Novozymes A/S	Mutants d'alpha-amylase dotés de propriétés altérées
US7151204B2 (en)	2001-01-09	2006-12-19	Monsanto Technology Llc	Maize chloroplast aldolase promoter compositions and methods for use thereof
ES2358991T3 (es)	2002-02-08	2011-05-17	Genencor International, Inc.	Procedimientos para producir productos finales a partir de sustratos de carbono.
WO2003066826A2 (fr)	2002-02-08	2003-08-14	Genencor International, Inc.	Methodes de production d'ethanol a partir de substrats de carbone
CN102210376B (zh)	2003-03-10	2014-12-31	波伊特研究股份有限公司	利用生淀粉生产乙醇的方法
US20040219649A1 (en)	2003-03-10	2004-11-04	Novozymes A/S	Alcohol product processes
US20060270007A1 (en)	2003-06-13	2006-11-30	Nielsen Anders V	Method for producing glucoamylases and their uses
WO2005003311A2 (fr)	2003-06-25	2005-01-13	Novozymes A/S	Enzymes de traitement d'amidon
ES2383366T3 (es)	2003-06-25	2012-06-20	Novozymes A/S	Enzimas para el tratamiento de almidón
EP1687419B1 (fr) *	2003-10-28	2010-02-03	Novozymes North America, Inc.	Enzymes hybrides
EP1831388A1 (fr) *	2004-12-22	2007-09-12	Novozymes A/S	Procede relatif a de l'amidon
EP1831384B1 (fr)	2004-12-22	2015-08-12	Novozymes A/S	Polypeptides presentant l'activite d'une glucoamylase, et polynucleotides encodant ces polypeptides
US20080280328A1 (en) *	2005-11-18	2008-11-13	Novozymes A/S	Glucoamylase Variants
PL2479267T3 (pl)	2006-12-21	2017-06-30	Basf Enzymes Llc	Amylazy i glukoamylazy, kwasy nukleinowe kodujące te związki oraz sposoby wytwarzania tych związków oraz stosowania ich
DK2483295T3 (en) *	2009-09-29	2016-02-22	Novozymes Inc	Polypeptides having cellulolytic enhancing activity and polynucleotides encoding them
ES2534066T3 (es) *	2009-11-30	2015-04-17	Novozymes A/S	Polipéptidos que tienen actividad de glucoamilasa y polinucleótidos que codifican los mismos
CA2847236A1 (fr) *	2011-09-09	2013-03-14	Novozymes A/S	Polypeptides ayant une activite alpha-amylase et polynucleotides codant pour ceux-ci

2012
- 2012-08-24 EP EP12828855.2A patent/EP2748188A4/fr not_active Withdrawn
- 2012-08-24 WO PCT/CN2012/080553 patent/WO2013029496A1/fr not_active Ceased
- 2012-08-24 CA CA2846690A patent/CA2846690A1/fr not_active Abandoned

Also Published As

Publication number	Publication date
EP2748188A4 (fr)	2015-03-18
WO2013029496A1 (fr)	2013-03-07
EP2748188A1 (fr)	2014-07-02

Legal Events

Date	Code	Title	Description
2017-08-24	EEER	Examination request	Effective date: 20170816
2019-10-07	FZDE	Dead	Effective date: 20190826

Publication	Publication Date	Title
US10316307B2 (en)	2019-06-11	Polypeptides having glucoamylase activity and polynucleotides encoding same
US10125380B2 (en)	2018-11-13	Polypeptides having glucoamylase activity and polynucleotides encoding same
EP2729572B1 (fr)	2019-03-20	Variants d'alpha-amylase et polynucléotides codant ces variants
CA2782036C (fr)	2019-01-15	Polypeptides possedant une activite de glucoamylase et polynucleotides codant pour ceux-ci
AU2010276470B2 (en)	2015-05-14	Polypeptides having glucoamylase activity and polynucleotides encoding same
CA2846690A1 (fr)	2013-03-07	Polypeptides ayant une activite glucoamylase et polynucleotides codant pour ces polypeptides
US8697412B2 (en)	2014-04-15	Polypeptides having glucoamylase activity and polynucleotides encoding same
US9309505B2 (en)	2016-04-12	Polypeptides having glucoamylase activity and polynucleotides encoding same
US9650620B2 (en)	2017-05-16	Polypeptides having glucoamylase activity and polynucleotides encoding same
CN103764670A (zh)	2014-04-30	具有葡糖淀粉酶活性的多肽和编码它的多核苷酸
US9567574B2 (en)	2017-02-14	Polypeptides having glucoamylase activity and polynucleotides encoding same
WO2013034097A1 (fr)	2013-03-14	Polypeptides ayant une activité glucoamylase et polynucléotides codant pour ceux-ci
WO2013029494A1 (fr)	2013-03-07	Polypeptides ayant une activité glucoamylase et polynucléotides codant pour ces polypeptides