CA2869472C - Preparation d'echantillon pour cytometrie de flux - Google Patents

Preparation d'echantillon pour cytometrie de flux Download PDF

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Publication number
CA2869472C
CA2869472C CA2869472A CA2869472A CA2869472C CA 2869472 C CA2869472 C CA 2869472C CA 2869472 A CA2869472 A CA 2869472A CA 2869472 A CA2869472 A CA 2869472A CA 2869472 C CA2869472 C CA 2869472C
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Canada
Prior art keywords
sample
background signal
reducing
molecule
nucleic acid
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CA2869472A
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English (en)
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CA2869472A1 (fr
Inventor
Xiao Li
Yongqiang Zhang
Kenneth Anthony Kopher
John D. Mantlo
William Alfred Pope
Song Shi
Axel A. Yup
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Becton Dickinson and Co
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Becton Dickinson and Co
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Publication of CA2869472A1 publication Critical patent/CA2869472A1/fr
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/02—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving viable microorganisms
    • C12Q1/04—Determining presence or kind of microorganism; Use of selective media for testing antibiotics or bacteriocides; Compositions containing a chemical indicator therefor
    • C12Q1/06—Quantitative determination
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6806—Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay

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  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Engineering & Computer Science (AREA)
  • Analytical Chemistry (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Genetics & Genomics (AREA)
  • Immunology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Toxicology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Plural Heterocyclic Compounds (AREA)

Abstract

La présente invention concerne des procédés et des réactifs qui permettent d'identifier et d'analyser au moins un micro-organisme (par exemple des bactéries) dans un échantillon, et de réduire l'intensité de signal de bruit de fond obtenue lors de l'analyse de l'échantillon par cytométrie de flux. L'échantillon est préparé par la combinaison de l'échantillon avec une molécule réduisant le signal de bruit de fond ou avec un marqueur d'acide nucléique lié de façon covalente à un extincteur de luminescence. Une partie de la matière particulaire dans l'échantillon peut être facultativement éliminée avec une résine avant le marquage par un marqueur d'acide nucléique.
CA2869472A 2012-04-05 2013-04-04 Preparation d'echantillon pour cytometrie de flux Active CA2869472C (fr)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US201261620823P 2012-04-05 2012-04-05
US61/620,823 2012-04-05
PCT/US2013/035291 WO2013152203A1 (fr) 2012-04-05 2013-04-04 Préparation d'échantillon pour cytométrie de flux

Publications (2)

Publication Number Publication Date
CA2869472A1 CA2869472A1 (fr) 2013-10-10
CA2869472C true CA2869472C (fr) 2017-07-11

Family

ID=49301050

Family Applications (1)

Application Number Title Priority Date Filing Date
CA2869472A Active CA2869472C (fr) 2012-04-05 2013-04-04 Preparation d'echantillon pour cytometrie de flux

Country Status (7)

Country Link
EP (1) EP2834367A4 (fr)
JP (1) JP6401146B2 (fr)
CN (1) CN104379760B (fr)
AU (1) AU2013243378B2 (fr)
CA (1) CA2869472C (fr)
IN (1) IN2014DN08957A (fr)
WO (1) WO2013152203A1 (fr)

Families Citing this family (13)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US11001881B2 (en) 2006-08-24 2021-05-11 California Institute Of Technology Methods for detecting analytes
US8048626B2 (en) 2006-07-28 2011-11-01 California Institute Of Technology Multiplex Q-PCR arrays
US11525156B2 (en) 2006-07-28 2022-12-13 California Institute Of Technology Multiplex Q-PCR arrays
US11560588B2 (en) 2006-08-24 2023-01-24 California Institute Of Technology Multiplex Q-PCR arrays
US9933341B2 (en) 2012-04-05 2018-04-03 Becton, Dickinson And Company Sample preparation for flow cytometry
CN105189774A (zh) * 2013-03-13 2015-12-23 贝克顿·迪金森公司 改进复合基质中微生物的分析的方法
CN104677809B (zh) * 2013-11-29 2018-01-02 中国人民解放军第二军医大学 隐球菌活力检测试剂盒及检测方法
US9708647B2 (en) 2015-03-23 2017-07-18 Insilixa, Inc. Multiplexed analysis of nucleic acid hybridization thermodynamics using integrated arrays
US9499861B1 (en) 2015-09-10 2016-11-22 Insilixa, Inc. Methods and systems for multiplex quantitative nucleic acid amplification
WO2017155858A1 (fr) 2016-03-07 2017-09-14 Insilixa, Inc. Identification de séquence d'acide nucléique à l'aide d'une extension de base unique cyclique en phase solide
CN107643238A (zh) * 2017-09-19 2018-01-30 中山大学附属第医院 一种定量检测血液循环微粒浓度的方法
US12196652B2 (en) * 2018-08-01 2025-01-14 Sartorius Bioanalytical Instruments, Inc. Methods, kits and stain compositions for flow cytometry evaluation of unassociated virus-size particles using multiple fluorogenic dyes
CN113924041B (zh) 2019-03-14 2024-12-03 因斯利克萨公司 基于时间门控的荧光检测的方法和系统

Family Cites Families (10)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5436134A (en) * 1993-04-13 1995-07-25 Molecular Probes, Inc. Cyclic-substituted unsymmetrical cyanine dyes
WO1996022531A1 (fr) * 1995-01-16 1996-07-25 Erkki Soini Procede de dosage multiparametrique biospecifique
US20030013849A1 (en) * 1999-10-29 2003-01-16 Ward William W. Renilla reniformis green fluorescent protein
RU2172512C1 (ru) * 2000-02-01 2001-08-20 Закрытое акционерное общество Научно-производственное объединение "ФоМос" Цветной спектрозональный галогенсеребряный фотографический материал
US6323337B1 (en) * 2000-05-12 2001-11-27 Molecular Probes, Inc. Quenching oligonucleotides
WO2002042267A2 (fr) * 2000-11-17 2002-05-30 Biomoda, Inc. Compositions et procedes pour detecter des etats precancereux dans des echantillons cellulaires et tissulaires au moyen de la 5, 10, 15, 20-tetrakis (carboxyphenyl) porphine
US7205100B2 (en) * 2002-10-10 2007-04-17 Advanced Analytical Technologies, Inc. Methods for reducing background fluorescence
US8481302B2 (en) * 2008-11-03 2013-07-09 General Electric Company Total bacteria monitoring system
GB201005939D0 (en) * 2010-04-09 2010-05-26 Biostatus Ltd Method of analysing a cell or other biological material containing nucleic acid
WO2011160887A1 (fr) * 2010-06-21 2011-12-29 Siemens Healthcare Diagnostics Inc. Désactivateur soluble pour réduire le bruit de fond dans les dosages de pcr quantitative

Also Published As

Publication number Publication date
CA2869472A1 (fr) 2013-10-10
AU2013243378B2 (en) 2019-04-11
WO2013152203A1 (fr) 2013-10-10
EP2834367A4 (fr) 2016-03-23
EP2834367A1 (fr) 2015-02-11
JP2015514403A (ja) 2015-05-21
IN2014DN08957A (fr) 2015-05-22
JP6401146B2 (ja) 2018-10-03
AU2013243378A1 (en) 2014-10-30
CN104379760B (zh) 2018-03-16
CN104379760A (zh) 2015-02-25

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