CA2876178C - Recombinant microorganisms expressing a non-specific acyltransferase make biodiesel - Google Patents

Recombinant microorganisms expressing a non-specific acyltransferase make biodiesel Download PDF

Info

Publication number
CA2876178C
CA2876178C CA2876178A CA2876178A CA2876178C CA 2876178 C CA2876178 C CA 2876178C CA 2876178 A CA2876178 A CA 2876178A CA 2876178 A CA2876178 A CA 2876178A CA 2876178 C CA2876178 C CA 2876178C
Authority
CA
Canada
Prior art keywords
microorganism
biodiesel
clostridium
nucleic acid
substrate
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Active
Application number
CA2876178A
Other languages
English (en)
French (fr)
Other versions
CA2876178A1 (en
Inventor
Fungmin Liew
Michael Koepke
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Lanzatech NZ Inc
Original Assignee
Lanzatech New Zealand Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Family has litigation
First worldwide family litigation filed litigation Critical https://patents.darts-ip.com/?family=49769067&utm_source=google_patent&utm_medium=platform_link&utm_campaign=public_patent_search&patent=CA2876178(C) "Global patent litigation dataset” by Darts-ip is licensed under a Creative Commons Attribution 4.0 International License.
Application filed by Lanzatech New Zealand Ltd filed Critical Lanzatech New Zealand Ltd
Publication of CA2876178A1 publication Critical patent/CA2876178A1/en
Application granted granted Critical
Publication of CA2876178C publication Critical patent/CA2876178C/en
Active legal-status Critical Current
Anticipated expiration legal-status Critical

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/64—Fats; Fatty oils; Ester-type waxes; Higher fatty acids, i.e. having at least seven carbon atoms in an unbroken chain bound to a carboxyl group; Oxidised oils or fats
    • C12P7/6436—Fatty acid esters
    • C12P7/649—Biodiesel, i.e. fatty acid alkyl esters
    • C—CHEMISTRY; METALLURGY
    • C10—PETROLEUM, GAS OR COKE INDUSTRIES; TECHNICAL GASES CONTAINING CARBON MONOXIDE; FUELS; LUBRICANTS; PEAT
    • C10L—FUELS NOT OTHERWISE PROVIDED FOR; NATURAL GAS; SYNTHETIC NATURAL GAS OBTAINED BY PROCESSES NOT COVERED BY SUBCLASSES C10G OR C10K; LIQUIFIED PETROLEUM GAS; USE OF ADDITIVES TO FUELS OR FIRES; FIRE-LIGHTERS
    • C10L1/00—Liquid carbonaceous fuels
    • C10L1/02—Liquid carbonaceous fuels essentially based on components consisting of carbon, hydrogen, and oxygen only
    • C10L1/026—Liquid carbonaceous fuels essentially based on components consisting of carbon, hydrogen, and oxygen only for compression ignition
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/74—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/1025—Acyltransferases (2.3)
    • C12N9/1029—Acyltransferases (2.3) transferring groups other than amino-acyl groups (2.3.1)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y203/00—Acyltransferases (2.3)
    • C12Y203/01—Acyltransferases (2.3) transferring groups other than amino-acyl groups (2.3.1)
    • C12Y203/0102—Diacylglycerol O-acyltransferase (2.3.1.20)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y203/00—Acyltransferases (2.3)
    • C12Y203/01—Acyltransferases (2.3) transferring groups other than amino-acyl groups (2.3.1)
    • C12Y203/01075—Long-chain-alcohol O-fatty-acyltransferase (2.3.1.75)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12R—INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
    • C12R2001/00—Microorganisms ; Processes using microorganisms
    • C12R2001/01—Bacteria or Actinomycetales ; using bacteria or Actinomycetales
    • C12R2001/145—Clostridium
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02E—REDUCTION OF GREENHOUSE GAS [GHG] EMISSIONS, RELATED TO ENERGY GENERATION, TRANSMISSION OR DISTRIBUTION
    • Y02E50/00—Technologies for the production of fuel of non-fossil origin
    • Y02E50/10—Biofuels, e.g. bio-diesel
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
    • Y02P20/00—Technologies relating to chemical industry
    • Y02P20/50—Improvements relating to the production of bulk chemicals
    • Y02P20/52—Improvements relating to the production of bulk chemicals using catalysts, e.g. selective catalysts

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Genetics & Genomics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Oil, Petroleum & Natural Gas (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • General Chemical & Material Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • Medicinal Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Enzymes And Modification Thereof (AREA)
CA2876178A 2012-06-21 2013-06-21 Recombinant microorganisms expressing a non-specific acyltransferase make biodiesel Active CA2876178C (en)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US201261662467P 2012-06-21 2012-06-21
US61/662,467 2012-06-21
PCT/NZ2013/000108 WO2013191567A1 (en) 2012-06-21 2013-06-21 Recombinant microorganisms make biodiesel

Publications (2)

Publication Number Publication Date
CA2876178A1 CA2876178A1 (en) 2013-12-27
CA2876178C true CA2876178C (en) 2017-02-28

Family

ID=49769067

Family Applications (1)

Application Number Title Priority Date Filing Date
CA2876178A Active CA2876178C (en) 2012-06-21 2013-06-21 Recombinant microorganisms expressing a non-specific acyltransferase make biodiesel

Country Status (13)

Country Link
US (1) US9347076B2 (2)
EP (1) EP2864470B2 (2)
JP (1) JP6466836B2 (2)
KR (1) KR102097844B1 (2)
CN (1) CN104640977B (2)
BR (1) BR112014031876B1 (2)
CA (1) CA2876178C (2)
ES (1) ES2655214T5 (2)
IN (1) IN2014DN10507A (2)
MY (1) MY164882A (2)
NO (1) NO2953671T3 (2)
PL (1) PL2864470T5 (2)
WO (1) WO2013191567A1 (2)

Families Citing this family (40)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB201112231D0 (en) * 2011-07-15 2011-08-31 Verdant Bioproducts Ltd Micro-organism
US20130323820A1 (en) 2012-06-01 2013-12-05 Lanzatech New Zealand Limited Recombinant microorganisms and uses therefor
US9347076B2 (en) 2012-06-21 2016-05-24 Lanzatech New Zealand Limited Recombinant microorganisms that make biodiesel
CN105940099A (zh) 2014-01-28 2016-09-14 朗泽科技新西兰有限公司 产生重组微生物的方法
JP6309304B2 (ja) * 2014-02-24 2018-04-11 積水化学工業株式会社 組換え細胞、並びに、１，４−ブタンジオールの生産方法
PL3209786T3 (pl) 2014-10-22 2023-10-30 Lanzatech Nz, Inc. Sposoby prowadzone w reaktorach wielostopniowych
EP4180806A1 (en) 2014-10-22 2023-05-17 LanzaTech NZ, Inc. Bioreactor and gas testing unit and method
US10131884B2 (en) 2015-02-23 2018-11-20 Lanzatech New Zealand Limited Recombinant acetogenic bacterium for the conversion of methane to products
BR112017025309A2 (pt) * 2015-05-27 2018-07-31 Lanzatech New Zealand Ltd micro-organismos geneticamente modificados para a fabricação de produtos derivados de corismato
MX391661B (es) 2015-10-13 2025-03-21 Lanzatech Nz Inc Bacteria diseñada por ingeniería genética que comprende una trayectoria de fermentación que genera energía
CA3010412C (en) 2015-12-03 2020-04-14 Lanzatech New Zealand Limited Arginine supplementation to improve efficiency in gas fermenting acetogens
US10358661B2 (en) 2015-12-28 2019-07-23 Lanzatech New Zealand Limited Microorganism with modified hydrogenase activity
ES2955708T3 (es) 2016-02-01 2023-12-05 Lanzatech Nz Inc Proceso integrado de fermentación y electrólisis
CA3015665C (en) 2016-02-26 2020-09-22 Lanzatech New Zealand Limited Crispr/cas systems for c1-fixing bacteria
EP3464559A4 (en) 2016-05-14 2020-02-12 Lanzatech, Inc. MODIFIED ALDEHYDE / FERREDOXIN OXYDOREDUCTASE MICROORGANISM AND RELATED METHODS
CN107217017B (zh) * 2017-05-27 2020-12-08 青岛农业大学 一株不动杆菌及其在石油降解中的应用
EP3679149B1 (en) 2017-09-08 2022-06-29 Lanzatech, Inc. Processes for metabolite production using hydrogen rich c1-containing substrates
MY196897A (en) 2017-12-19 2023-05-09 Lanzatech Inc Microorganisms and methods for the biological production of ethylene glycol
CN111683731B (zh) 2018-02-12 2022-09-02 朗泽科技有限公司 用于提高碳转化效率的工艺
EP3781668B1 (en) 2018-04-20 2023-10-04 Lanzatech, Inc. Fermentation process for producing ethanol
CA3120151C (en) 2018-11-19 2024-04-30 Lanzatech, Inc. Integration of fermentation and gasification
CN112969795A (zh) 2019-02-08 2021-06-15 朗泽科技有限公司 用于回收沸点接近的产物的方法
CN112955560A (zh) 2019-07-11 2021-06-11 朗泽科技有限公司 优化气体利用的方法
CN111088193B (zh) * 2020-01-10 2022-09-09 南京工业大学 一种提高食甲基丁酸杆菌电转频率的方法
US11932818B2 (en) 2020-03-16 2024-03-19 Lanzatech, Inc. Tail gas of gas fermentation to dry gasification feedstock
AU2021237368B2 (en) 2020-03-18 2025-05-08 Lanzatech, Inc. Fermentative production of 2-phenylethanol from gaseous substrates
EP4143325A4 (en) 2020-04-29 2024-09-11 Lanzatech, Inc. FERMENTATIVE PRODUCTION OF BETA-KETOADIPATE FROM GASEOUS SUBSTRATES
CA3224707A1 (en) 2020-06-06 2021-12-09 Lanzatech, Inc. Microorganism with knock-in at acetolactate decarboxylase gene locus
CN116348603B (zh) 2021-02-08 2024-07-02 朗泽科技有限公司 重组微生物及其用途
JP2024513395A (ja) 2021-04-09 2024-03-25 ランザテク，インコーポレイテッド 供給原料を提供するためのプロセス及び装置
US12241105B2 (en) 2021-07-20 2025-03-04 Lanzatech, Inc. Recombinant microorganisms and uses therefor
TW202307202A (zh) 2021-08-06 2023-02-16 美商朗澤科技有限公司 用於改良乙二醇之生物產生的微生物及方法
US12091648B2 (en) 2021-11-03 2024-09-17 Lanzatech, Inc. System and method for generating bubbles in a vessel
US12280331B2 (en) 2022-04-29 2025-04-22 Lanzatech, Inc. Low residence time gas separator
US12077800B2 (en) 2022-06-16 2024-09-03 Lanzatech, Inc. Liquid distributor system and process of liquid distribution
WO2023250382A1 (en) 2022-06-21 2023-12-28 Lanzatech, Inc. Microorganisms and methods for the continuous co-production of tandem repeat proteins and chemical products from c1-substrates
AU2023289395A1 (en) 2022-06-21 2025-01-09 Lanzatech, Inc. Microorganisms and methods for the continuous co-production of high-value, specialized proteins and chemical products from c1-substrates
WO2024036187A1 (en) 2022-08-10 2024-02-15 Lanzatech, Inc. Carbon sequestration in soils with production of chemical products
WO2024253883A1 (en) 2023-06-05 2024-12-12 Lanzatech, Inc. Integrated gas fermentation and carbon black processes
WO2024253882A1 (en) 2023-06-05 2024-12-12 Lanzatech, Inc. Integrated gas fermentation

Family Cites Families (21)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5173429A (en) 1990-11-09 1992-12-22 The Board Of Trustees Of The University Of Arkansas Clostridiumm ljungdahlii, an anaerobic ethanol and acetate producing microorganism
US5593886A (en) 1992-10-30 1997-01-14 Gaddy; James L. Clostridium stain which produces acetic acid from waste gases
UA72220C2 (uk) 1998-09-08 2005-02-15 Байоенджініерінг Рісорсиз, Інк. Незмішувана з водою суміш розчинник/співрозчинник для екстрагування оцтової кислоти, спосіб одержання оцтової кислоти (варіанти), спосіб анаеробного мікробного бродіння для одержання оцтової кислоти (варіанти), модифікований розчинник та спосіб його одержання
DK1303629T3 (da) 2000-07-25 2006-10-30 Emmaus Foundation Inc Fremgangsmåde til forögelse af ethanolproduktion fra mikrobiel fermetering
DE102004052115A1 (de) * 2004-10-26 2006-04-27 Westfälische Wilhelms-Universität Münster Mikroorganismen zur Herstellung von Fettsäureestern, Verfahren zu deren Herstellung und deren Verwendung
EP2024491B1 (en) * 2006-05-19 2014-11-19 LS9, Inc. Production of fatty acids and derivatives thereof
US7704723B2 (en) 2006-08-31 2010-04-27 The Board Of Regents For Oklahoma State University Isolation and characterization of novel clostridial species
CA3178593A1 (en) * 2007-03-28 2008-10-02 Genomatica, Inc. Enhanced production of fatty acid derivatives
US20100199548A1 (en) * 2007-07-06 2010-08-12 Ls9, Inc. Systems and methods for the production of fatty esters
CN102876609A (zh) 2007-11-13 2013-01-16 兰扎泰克新西兰有限公司 新型菌及其使用方法
GB0801787D0 (en) * 2008-01-31 2008-03-05 Reclaim Resources Ltd Apparatus and method for treating waste
US8048654B2 (en) * 2010-06-09 2011-11-01 Joule Unlimited Technologies, Inc. Methods and compositions for the recombinant biosynthesis of fatty acids and esters
US20100071259A1 (en) * 2008-08-18 2010-03-25 Ls9, Inc. Systems and methods for production of mixed fatty esters
CA2758298A1 (en) * 2009-04-10 2010-10-14 Ls9, Inc. Production of commercial biodiesel from genetically modified microorganisms
BRPI1009980A2 (pt) 2009-05-01 2015-08-25 Univ California Produtos de ésteres de ácidos graxos a partir de polímeros de biomassa
US8143037B2 (en) 2010-03-19 2012-03-27 Coskata, Inc. Ethanologenic Clostridium species, Clostridium coskatii
CN103282505A (zh) * 2010-08-26 2013-09-04 新西兰郎泽科技公司 通过发酵产生乙醇和乙烯的方法
US20110236941A1 (en) * 2010-10-22 2011-09-29 Lanzatech New Zealand Limited Recombinant microorganism and methods of production thereof
BR112013021524A2 (pt) 2011-02-25 2017-06-13 Lanzatech New Zealand Ltd micro-organismos recombinantes e usos dos mesmos
CN102492672A (zh) * 2011-11-26 2012-06-13 宁波大学 一种催化二酰甘油酰基化的酶
US9347076B2 (en) 2012-06-21 2016-05-24 Lanzatech New Zealand Limited Recombinant microorganisms that make biodiesel

Also Published As

Publication number Publication date
MY164882A (en) 2018-01-30
ES2655214T5 (es) 2021-10-11
IN2014DN10507A (2) 2015-08-21
CA2876178A1 (en) 2013-12-27
JP2015519918A (ja) 2015-07-16
WO2013191567A1 (en) 2013-12-27
CN104640977A (zh) 2015-05-20
EP2864470A4 (en) 2015-12-16
EP2864470A1 (en) 2015-04-29
EP2864470B2 (en) 2020-12-23
EP2864470B1 (en) 2017-10-04
PL2864470T5 (pl) 2021-05-17
CN104640977B (zh) 2019-04-12
KR20150023651A (ko) 2015-03-05
US9347076B2 (en) 2016-05-24
JP6466836B2 (ja) 2019-02-06
ES2655214T3 (es) 2018-02-19
KR102097844B1 (ko) 2020-04-06
NO2953671T3 (2) 2018-03-17
BR112014031876B1 (pt) 2022-05-10
BR112014031876A2 (pt) 2017-08-01
PL2864470T3 (pl) 2018-05-30
US20130344547A1 (en) 2013-12-26

Similar Documents

Publication Publication Date Title
CA2876178C (en) Recombinant microorganisms expressing a non-specific acyltransferase make biodiesel
CA2813431C (en) Production of butanol from carbon monoxide by a recombinant microorganism
CA3051235C (en) Genetically engineered bacterium comprising energy-generating fermentation pathway
CA2995872C (en) Recombinant clostridium bacterium and uses thereof in acetone production
CA2874832C (en) Recombinant microorganisms and uses therefor
CA2882276E (en) Recombinant microorganisms expressing a stereospecific diol dehydratase enzyme
EP4162056A1 (en) Microorganism with knock-in at acetolactate decarboxylase gene locus
JP2014161252A (ja) 組換え細胞

Legal Events

Date Code Title Description
EEER Examination request

Effective date: 20141209

W00 Other event occurred

Free format text: ST27 STATUS EVENT CODE: A-4-4-W10-W00-W100 (AS PROVIDED BY THE NATIONAL OFFICE); EVENT TEXT: LETTER SENT

Effective date: 20251009

H13 Ip right lapsed

Free format text: ST27 STATUS EVENT CODE: N-4-6-H10-H13-H100 (AS PROVIDED BY THE NATIONAL OFFICE); EVENT TEXT: MAINTENANCE FEE AND LATE FEE NOT PAID BY DEADLINE OF NOTICE

Effective date: 20260203

W00 Other event occurred

Free format text: ST27 STATUS EVENT CODE: N-6-6-W10-W00-W100 (AS PROVIDED BY THE NATIONAL OFFICE); EVENT TEXT: LETTER SENT

Effective date: 20260205