CA2936646C - Methodes et compositions pour des sequences guidant le ciblage de cas9 - Google Patents

Methodes et compositions pour des sequences guidant le ciblage de cas9 Download PDF

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Publication number
CA2936646C
CA2936646C CA2936646A CA2936646A CA2936646C CA 2936646 C CA2936646 C CA 2936646C CA 2936646 A CA2936646 A CA 2936646A CA 2936646 A CA2936646 A CA 2936646A CA 2936646 C CA2936646 C CA 2936646C
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sequence
nucleic acid
acid construct
nucleotides
stitch
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CA2936646A1 (fr
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Rodolphe Barrangou
Kurt M. SELLE
Alexandra E. BRINER
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North Carolina State University
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North Carolina State University
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/113—Non-coding nucleic acids modulating the expression of genes, e.g. antisense oligonucleotides; Antisense DNA or RNA; Triplex- forming oligonucleotides; Catalytic nucleic acids, e.g. ribozymes; Nucleic acids used in co-suppression or gene silencing
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2310/00—Structure or type of the nucleic acid
    • C12N2310/10—Type of nucleic acid
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2310/00—Structure or type of the nucleic acid
    • C12N2310/10—Type of nucleic acid
    • C12N2310/20—Type of nucleic acid involving clustered regularly interspaced short palindromic repeats [CRISPR]

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  • Genetics & Genomics (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Health & Medical Sciences (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • Wood Science & Technology (AREA)
  • Organic Chemistry (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Zoology (AREA)
  • Physics & Mathematics (AREA)
  • Microbiology (AREA)
  • Plant Pathology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Biophysics (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Peptides Or Proteins (AREA)

Abstract

La présente invention concerne des méthodes et des compositions pour l'édition du génome et le ciblage de l'ADN de protéines.
CA2936646A 2014-01-24 2015-01-23 Methodes et compositions pour des sequences guidant le ciblage de cas9 Active CA2936646C (fr)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US201461931515P 2014-01-24 2014-01-24
US61/931,515 2014-01-24
PCT/US2015/012747 WO2015112896A2 (fr) 2014-01-24 2015-01-23 Méthodes et compositions pour des séquences guidant le ciblage de cas9

Publications (2)

Publication Number Publication Date
CA2936646A1 CA2936646A1 (fr) 2015-07-30
CA2936646C true CA2936646C (fr) 2024-04-30

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CA2936646A Active CA2936646C (fr) 2014-01-24 2015-01-23 Methodes et compositions pour des sequences guidant le ciblage de cas9

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EP (1) EP3097212A4 (fr)
JP (4) JP2017503514A (fr)
CA (1) CA2936646C (fr)
WO (1) WO2015112896A2 (fr)

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Also Published As

Publication number Publication date
JP7788744B2 (ja) 2025-12-19
EP3097212A4 (fr) 2017-10-04
JP2022106883A (ja) 2022-07-20
JP2017503514A (ja) 2017-02-02
CA2936646A1 (fr) 2015-07-30
WO2015112896A2 (fr) 2015-07-30
JP2024041934A (ja) 2024-03-27
WO2015112896A3 (fr) 2015-10-29
WO2015112896A9 (fr) 2015-11-26
JP2020103295A (ja) 2020-07-09
JP7429057B2 (ja) 2024-02-07
EP3097212A2 (fr) 2016-11-30

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