CA2975789C - Methode de synthese d'acide nucleique - Google Patents

Methode de synthese d'acide nucleique

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Publication number
CA2975789C
CA2975789C CA2975789A CA2975789A CA2975789C CA 2975789 C CA2975789 C CA 2975789C CA 2975789 A CA2975789 A CA 2975789A CA 2975789 A CA2975789 A CA 2975789A CA 2975789 C CA2975789 C CA 2975789C
Authority
CA
Canada
Prior art keywords
tdt
nucleic acid
deoxynucleotidyl transferase
initiator
dna
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Active
Application number
CA2975789A
Other languages
English (en)
Other versions
CA2975789A1 (fr
Inventor
Michael C. Chen
Radu A. Lazar
Jiahao Huang
Gordon R. McInroy
Original Assignee
Nuclera Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from GBGB1502152.0A external-priority patent/GB201502152D0/en
Application filed by Nuclera Ltd filed Critical Nuclera Ltd
Publication of CA2975789A1 publication Critical patent/CA2975789A1/fr
Application granted granted Critical
Publication of CA2975789C publication Critical patent/CA2975789C/fr
Active legal-status Critical Current
Anticipated expiration legal-status Critical

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Abstract

La présente invention concerne l'utilisation d'enzymes désoxynucléotidyle transférases terminales (TdT) spécifiques dans un procédé de synthèse d'acides nucléiques, des procédés de synthèse d'acides nucléiques, et l'utilisation de trousses comprenant lesdites enzymes dans un procédé de synthèse d'acides nucléiques. L'invention concerne également l'utilisation de désoxynucléotidyle transférases terminales et de nucléotides triphosphates bloqués en terminaison 3' dans un procédé de synthèse d'acides nucléiques indépendant de la matrice.
CA2975789A 2015-02-10 2016-02-10 Methode de synthese d'acide nucleique Active CA2975789C (fr)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
GB1502152.0 2015-02-10
GBGB1502152.0A GB201502152D0 (en) 2015-02-10 2015-02-10 Novel use
PCT/GB2016/050301 WO2016128731A1 (fr) 2015-02-10 2016-02-10 Nouvelle utilisation

Publications (2)

Publication Number Publication Date
CA2975789A1 CA2975789A1 (fr) 2016-08-18
CA2975789C true CA2975789C (fr) 2026-02-10

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