CA2992170C - Promoteurs pg1-x artificiels - Google Patents

Promoteurs pg1-x artificiels Download PDF

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Publication number
CA2992170C
CA2992170C CA2992170A CA2992170A CA2992170C CA 2992170 C CA2992170 C CA 2992170C CA 2992170 A CA2992170 A CA 2992170A CA 2992170 A CA2992170 A CA 2992170A CA 2992170 C CA2992170 C CA 2992170C
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Prior art keywords
promoter
seq
nucleotide sequence
set forth
sequence set
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CA2992170A
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CA2992170A1 (fr
Inventor
Diethard Mattanovich
Brigitte Gasser
Roland PRIELHOFER
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Lonza AG
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Lonza AG
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/80—Vectors or expression systems specially adapted for eukaryotic hosts for fungi
    • C12N15/81—Vectors or expression systems specially adapted for eukaryotic hosts for fungi for yeasts
    • C12N15/815—Vectors or expression systems specially adapted for eukaryotic hosts for fungi for yeasts for yeasts other than Saccharomyces
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/14—Fungi; Culture media therefor
    • C12N1/16—Yeasts; Culture media therefor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P21/00—Preparation of peptides or proteins
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P21/00—Preparation of peptides or proteins
    • C12P21/02—Preparation of peptides or proteins having a known sequence of two or more amino acids, e.g. glutathione
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2830/00—Vector systems having a special element relevant for transcription
    • C12N2830/001—Vector systems having a special element relevant for transcription controllable enhancer/promoter combination

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  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Genetics & Genomics (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Biotechnology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Mycology (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Plant Pathology (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Medicinal Chemistry (AREA)
  • Botany (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Virology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Saccharide Compounds (AREA)
  • Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)

Abstract

La présente invention concerne un promoteur pG1-x isolé et/ou artificiel, qui est un variant fonctionnel du promoteur pG1 à source de carbone régulable de Pichia pastoris identifié par SEQ ID 1, ledit promoteur pG1-x étant constitué d'au moins une partie de SEQ ID 1 avec une longueur d'au moins 293 pb, caractérisé par les régions de promoteur suivantes : a) au moins une région régulatrice centrale comprenant les séquences nucléotidiques SEQ ID 2 et SEQ ID 3 ; et b) une région régulatrice non centrale, qui est n'importe quelle région à l'intérieur de la séquence du promoteur pG1-x autre que la région régulatrice centrale ; le promoteur pG1-x comprenant au moins une mutation dans n'importe quelle région du promoteur et une identité de séquence d'au moins 80 % dans SEQ ID 2 et SEQ ID 3, et une identité de séquence d'au moins 50 % dans n'importe quelle région autre que SEQ ID 2 ou SEQ ID 3 ; le promoteur pG1-x étant en outre caractérisé en ce qu'il présente une résistance de promoteur et un rapport d'induction égaux ou supérieurs à ceux du promoteur pG1, la résistance de promoteur étant est au moins 1,1 fois accrue à l'état induit par rapport au promoteur pG1, et/ou le rapport d'induction étant au moins 1,1 fois accru par comparaison avec le promoteur pG1.
CA2992170A 2015-08-05 2016-08-05 Promoteurs pg1-x artificiels Active CA2992170C (fr)

Applications Claiming Priority (5)

Application Number Priority Date Filing Date Title
EPPCT/EP2015/068024 2015-08-05
EP2015068024 2015-08-05
EP16163932 2016-04-05
EP16163932.3 2016-04-05
PCT/EP2016/068784 WO2017021541A1 (fr) 2015-08-05 2016-08-05 Variants de promoteur

Publications (2)

Publication Number Publication Date
CA2992170A1 CA2992170A1 (fr) 2017-02-09
CA2992170C true CA2992170C (fr) 2022-08-16

Family

ID=56684642

Family Applications (1)

Application Number Title Priority Date Filing Date
CA2992170A Active CA2992170C (fr) 2015-08-05 2016-08-05 Promoteurs pg1-x artificiels

Country Status (13)

Country Link
US (2) US10752907B2 (fr)
EP (1) EP3332005B1 (fr)
JP (1) JP6833812B2 (fr)
KR (1) KR102253479B1 (fr)
CN (1) CN108350447B (fr)
AU (1) AU2016303026B2 (fr)
BR (1) BR112018002395A2 (fr)
CA (1) CA2992170C (fr)
DK (1) DK3332005T3 (fr)
ES (1) ES2902044T3 (fr)
IL (1) IL257000B (fr)
TW (1) TW201718859A (fr)
WO (1) WO2017021541A1 (fr)

Families Citing this family (25)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR102128957B1 (ko) 2011-10-07 2020-07-02 론자 리미티드 조절가능한 프로모터
BR112020016258A2 (pt) 2018-02-12 2020-12-15 Lonza Ltd Uma célula hospedeira eucariótica modificada geneticamente projetada para reduzir a produção de proteínas da célula hospedeira, método de produzir uma proteína de interesse usando a célula hospedeira, método para reduzir a contaminação por proteína da célula hospedeira
EP3536784A1 (fr) 2018-03-05 2019-09-11 ACIB GmbH Cellule hôte conçue pour améliorer la production de métabolites
WO2019236451A1 (fr) * 2018-06-07 2019-12-12 Basf Se Promoteur pour levure
WO2020068018A1 (fr) 2018-09-24 2020-04-02 Orta Dogu Teknik Universitesi Variants de promoteur aox1 modifiés
US12091658B2 (en) * 2018-09-24 2024-09-17 Orta Dogu Teknik Universitesi Alcohol dehydrogenase 2 (ADH2) promoter variants by promoter engineering
ES2921137T5 (en) * 2019-01-11 2025-05-06 Lonza Ag Carbon-source regulated protein production in a recombinant host cell
EP3690052B1 (fr) * 2019-01-29 2023-04-19 Clariant Produkte (Deutschland) GmbH Promoteur chimérique pour une utilisation dans l'ingénierie métabolique
WO2020200414A1 (fr) 2019-04-01 2020-10-08 Lonza Ltd Production de protéines dans de la levure mut-méthylotrophe
WO2020200415A1 (fr) 2019-04-01 2020-10-08 Lonza Ltd Levure mut-méthylotrophe
US12270034B2 (en) 2019-04-01 2025-04-08 Universität Für Bodenkultur Wien Mut- methylotrophic yeast
US11618900B2 (en) * 2019-11-01 2023-04-04 The Johns Hopkins University Modulating SYNGAP
ES3034258T3 (en) 2020-04-01 2025-08-14 Lonza Ag Helper factors for expressing proteins in yeast
US20240026412A1 (en) * 2020-04-09 2024-01-25 Valneva Austria Gmbh Improved methods of producing a lipidated protein
KR20230075436A (ko) 2020-09-30 2023-05-31 론자 리미티드 번역 인자를 과발현하는 숙주 세포
EP4291659A1 (fr) 2021-02-12 2023-12-20 Boehringer Ingelheim RCV GmbH & Co KG Peptides signaux pour une sécrétion accrue de protéines
CN114657190B (zh) * 2022-04-06 2023-08-29 暨南大学 Msn2p作为负调控因子在提高宿主细胞中蛋白表达中的应用
CN114908092B (zh) * 2022-05-12 2023-07-18 华南理工大学 一种长效、高强度的杂合启动子及其重组菌株和应用
EP4353825A1 (fr) * 2022-10-10 2024-04-17 bisy GmbH Sequences promotrices modifiees
EP4638756A1 (fr) 2022-12-20 2025-10-29 Lonza Ltd. Cellules hôtes à inactivation de pat1 permettant une productivité accrue de protéine spécifique
EP4726051A1 (fr) 2023-06-06 2026-04-15 FUJIFILM Corporation Levure recombinante, procédé de production de protéine cible et kit et procédé de production de levure recombinante
WO2024256996A1 (fr) * 2023-06-15 2024-12-19 Danstar Ferment Ag Promoteurs sensibles a la source de carbone c2
WO2025247966A1 (fr) 2024-05-29 2025-12-04 Mybios Gmbh Procédé de production de protéines dans une cellule hôte de levure méthylotrophe
WO2026027251A1 (fr) 2024-08-01 2026-02-05 Boehringer Ingelheim Rcv Gmbh & Co Kg Peptides signaux permettant d'augmenter la sécrétion de protéines
BE1032887B1 (nl) * 2024-08-26 2026-04-14 Anheuser Busch Inbev Sa Geoptimaliseerd promotorontwerp voor industriëe toepassing

Family Cites Families (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
AT501955B1 (de) 2005-02-23 2007-08-15 Univ Graz Tech Mutierte aox1-promotoren
US8105811B2 (en) * 2008-06-13 2012-01-31 Wisconsin Alumni Research Foundation Sugar transport sequences, yeast strains having improved sugar uptake, and methods of use
JP2013509180A (ja) * 2009-10-30 2013-03-14 メルク・シャープ・エンド・ドーム・コーポレイション 改善された分泌効率を有する組換えタンパク質の産生方法
KR102128957B1 (ko) * 2011-10-07 2020-07-02 론자 리미티드 조절가능한 프로모터
EP3130598B1 (fr) * 2012-10-29 2020-02-12 Lonza Ltd Séquences d'expression

Also Published As

Publication number Publication date
EP3332005B1 (fr) 2021-09-22
CA2992170A1 (fr) 2017-02-09
WO2017021541A1 (fr) 2017-02-09
AU2016303026B2 (en) 2020-10-22
HK1251256A1 (zh) 2019-01-25
AU2016303026A1 (en) 2018-02-15
DK3332005T3 (da) 2022-01-03
CN108350447B (zh) 2021-08-31
US20180223293A1 (en) 2018-08-09
JP6833812B2 (ja) 2021-02-24
IL257000B (en) 2022-06-01
KR20180037237A (ko) 2018-04-11
JP2018522565A (ja) 2018-08-16
US20200347391A1 (en) 2020-11-05
EP3332005A1 (fr) 2018-06-13
CN108350447A (zh) 2018-07-31
IL257000A (en) 2018-03-29
US10752907B2 (en) 2020-08-25
TW201718859A (zh) 2017-06-01
ES2902044T3 (es) 2022-03-24
US12522837B2 (en) 2026-01-13
BR112018002395A2 (pt) 2018-09-18
KR102253479B1 (ko) 2021-05-21

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