CA2993401C - Dispositif de detection d'activite metabolique electrochimique - Google Patents

Dispositif de detection d'activite metabolique electrochimique Download PDF

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Publication number
CA2993401C
CA2993401C CA2993401A CA2993401A CA2993401C CA 2993401 C CA2993401 C CA 2993401C CA 2993401 A CA2993401 A CA 2993401A CA 2993401 A CA2993401 A CA 2993401A CA 2993401 C CA2993401 C CA 2993401C
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well
bacteria
metabolic activity
target cells
resazurin
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Expired - Fee Related
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CA2993401A
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CA2993401A1 (fr
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Shana Olwyn KELLEY
Edward Hartley Sargent
Justin David BESANT
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University of Toronto
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University of Toronto
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    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
    • B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
    • B01L3/502761—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip specially adapted for handling suspended solids or molecules independently from the bulk fluid flow, e.g. for trapping or sorting beads or physically stretching molecules
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/02—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving viable microorganisms
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N1/00—Sampling; Preparing specimens for investigation
    • G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
    • G01N1/40—Concentrating samples
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/483—Physical analysis of biological material
    • G01N33/487—Physical analysis of biological material of liquid biological material
    • G01N33/48707—Physical analysis of biological material of liquid biological material by electrical means
    • G01N33/48735—Investigating suspensions of cells, e.g. measuring microbe concentration
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/483—Physical analysis of biological material
    • G01N33/487—Physical analysis of biological material of liquid biological material
    • G01N33/49—Blood
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/483—Physical analysis of biological material
    • G01N33/487—Physical analysis of biological material of liquid biological material
    • G01N33/493—Physical analysis of biological material of liquid biological material urine
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/5005—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2200/00—Solutions for specific problems relating to chemical or physical laboratory apparatus
    • B01L2200/06—Fluid handling related problems
    • B01L2200/0647—Handling flowable solids, e.g. microscopic beads, cells, particles
    • B01L2200/0668—Trapping microscopic beads
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2200/00—Solutions for specific problems relating to chemical or physical laboratory apparatus
    • B01L2200/06—Fluid handling related problems
    • B01L2200/0673—Handling of plugs of fluid surrounded by immiscible fluid
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/06—Auxiliary integrated devices, integrated components
    • B01L2300/0627—Sensor or part of a sensor is integrated
    • B01L2300/0645—Electrodes
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/06—Auxiliary integrated devices, integrated components
    • B01L2300/0681—Filter
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/08—Geometry, shape and general structure
    • B01L2300/0809—Geometry, shape and general structure rectangular shaped
    • B01L2300/0816—Cards, e.g. flat sample carriers usually with flow in two horizontal directions
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/08—Geometry, shape and general structure
    • B01L2300/0861—Configuration of multiple channels and/or chambers in a single devices
    • B01L2300/0864—Configuration of multiple channels and/or chambers in a single devices comprising only one inlet and multiple receiving wells, e.g. for separation, splitting
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2400/00—Moving or stopping fluids
    • B01L2400/04—Moving fluids with specific forces or mechanical means
    • B01L2400/0475—Moving fluids with specific forces or mechanical means specific mechanical means and fluid pressure
    • B01L2400/0487—Moving fluids with specific forces or mechanical means specific mechanical means and fluid pressure fluid pressure, pneumatics
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2400/00—Moving or stopping fluids
    • B01L2400/06—Valves, specific forms thereof
    • B01L2400/0633—Valves, specific forms thereof with moving parts

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Physics & Mathematics (AREA)
  • Biomedical Technology (AREA)
  • Immunology (AREA)
  • General Health & Medical Sciences (AREA)
  • Analytical Chemistry (AREA)
  • Hematology (AREA)
  • Biochemistry (AREA)
  • Molecular Biology (AREA)
  • Urology & Nephrology (AREA)
  • Pathology (AREA)
  • General Physics & Mathematics (AREA)
  • Organic Chemistry (AREA)
  • Medicinal Chemistry (AREA)
  • Biophysics (AREA)
  • Food Science & Technology (AREA)
  • Biotechnology (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Microbiology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Fluid Mechanics (AREA)
  • Dispersion Chemistry (AREA)
  • Clinical Laboratory Science (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Cell Biology (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Genetics & Genomics (AREA)
  • General Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Ecology (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

Des méthodes et des dispositifs permettent de détecter lactivité métabolique des cellules cibles dans un échantillon. Les cellules cibles sont concentrées dans un puits dune capacité dun nanolitre comprenant un microfiltre. Un composé de signalement qui présente un changement détat électrochimique en réponse à lactivité métabolique des cellules cibles est introduit. Lactivité métabolique ou la viabilité des cellules cibles est détectée selon un changement déterminé dans létat électrochimique du contenu du puits.
CA2993401A 2014-10-28 2015-10-28 Dispositif de detection d'activite metabolique electrochimique Expired - Fee Related CA2993401C (fr)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US201462069601P 2014-10-28 2014-10-28
US62/069,601 2014-10-28
CA2966007A CA2966007C (fr) 2014-10-28 2015-10-28 Dispositif de detection d'activite metabolique electrochimique

Related Parent Applications (1)

Application Number Title Priority Date Filing Date
CA2966007A Division CA2966007C (fr) 2014-10-28 2015-10-28 Dispositif de detection d'activite metabolique electrochimique

Publications (2)

Publication Number Publication Date
CA2993401A1 CA2993401A1 (fr) 2016-05-06
CA2993401C true CA2993401C (fr) 2018-09-18

Family

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Family Applications (2)

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CA2966007A Expired - Fee Related CA2966007C (fr) 2014-10-28 2015-10-28 Dispositif de detection d'activite metabolique electrochimique
CA2993401A Expired - Fee Related CA2993401C (fr) 2014-10-28 2015-10-28 Dispositif de detection d'activite metabolique electrochimique

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CA2966007A Expired - Fee Related CA2966007C (fr) 2014-10-28 2015-10-28 Dispositif de detection d'activite metabolique electrochimique

Country Status (5)

Country Link
US (2) US10449542B2 (fr)
EP (1) EP3213067A4 (fr)
CN (1) CN107209170A (fr)
CA (2) CA2966007C (fr)
WO (1) WO2016065475A1 (fr)

Families Citing this family (13)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN107209170A (zh) * 2014-10-28 2017-09-26 多伦多大学管理委员会 电化学代谢活性检测装置
CN108350408B (zh) * 2015-08-25 2022-12-16 阿威尔斯医疗公司 用于检测流体样品中有活力的微生物的设备、系统和方法
DK3443337T3 (da) * 2016-04-12 2020-12-14 Univ Danmarks Tekniske Elektrokemisk indretning til detektering af udvalgte quorumsensorsignaler
WO2018111234A1 (fr) 2016-12-13 2018-06-21 Avails Medical, Inc. Dispositifs, systèmes et procédés de détection de la présence de bactéries d'hydrolyse d'un antibiotique ss-lactamine dans un échantillon
WO2019005296A1 (fr) 2017-06-27 2019-01-03 Avails Medical, Inc. Appareil, systèmes et procédés permettant de déterminer la sensibilité de micro-organismes à des anti-infectieux
JP7209701B2 (ja) 2017-10-03 2023-01-20 アベイルズ メディカル，インコーポレイテッド レドックス反応に基づいて微生物の濃度及び抗感染剤に対する微生物の感受性を決定する装置、システム、及び方法
CN117451472A (zh) 2017-12-05 2024-01-26 阿威尔斯医疗公司 制备用于下游测试的包含确定浓度的感染原的输出样品
WO2020117650A1 (fr) 2018-12-03 2020-06-11 Avails Medical, Inc. Appareils, systèmes, et procédés de quantification d'agents infectieux
CN110530957B (zh) * 2019-08-27 2021-01-29 江南大学 一种细菌耐药性电化学检测方法
CN114746741A (zh) * 2019-10-18 2022-07-12 分离生物公司 在厌氧条件下使用试卤灵监测细胞代谢活性
EP4264237A4 (fr) * 2020-12-17 2024-10-23 Isolation Bio Inc. Criblage de microbes fluorescents à l'aide d'un dispositif microfabriqué
JPWO2024004371A1 (fr) * 2022-06-28 2024-01-04
NL2037988B1 (en) * 2024-06-19 2026-01-14 Nostics B V Method and device for analysing antimicrobial compound susceptibility of pathogenic microorganisms in body fluid samples

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US7004184B2 (en) * 2000-07-24 2006-02-28 The Reagents Of The University Of Michigan Compositions and methods for liquid metering in microchannels
CA2495021A1 (fr) * 2001-08-06 2003-07-03 Vanderbilt University Systeme et procedes de mesure d'au moins un taux metabolique de plusieurs cellules
WO2004065618A2 (fr) * 2003-01-16 2004-08-05 Thermogenic Imaging Procedes et dispositifs permettant de controler le metabolisme cellulaire dans des chambres de retenue de cellules
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AU2009262959A1 (en) * 2008-06-27 2009-12-30 Massachusetts Institute Of Technology Microfluidic droplets for metabolic engineering and other applications
WO2010011939A2 (fr) * 2008-07-25 2010-01-28 Life Bioscience, Inc. Plaques, procédés et systèmes d'analyse comprenant un ou plusieurs éléments gravés
US8888969B2 (en) * 2008-09-02 2014-11-18 The Governing Council Of The University Of Toronto Nanostructured microelectrodes and biosensing devices incorporating the same
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EP2859340B1 (fr) * 2012-05-24 2017-05-03 The Governing Council Of The University Of Toronto Systèmes et procédés de détection électrochimique multiplexée
WO2014166000A1 (fr) * 2013-04-11 2014-10-16 The Governing Council Of The University Of Toronto Dispositif de prélèvement de particules dans un courant
US10527626B2 (en) * 2013-07-05 2020-01-07 University Of Washington Through Its Center For Commercialization Methods, compositions and systems for microfluidic assays
CN107209170A (zh) * 2014-10-28 2017-09-26 多伦多大学管理委员会 电化学代谢活性检测装置

Also Published As

Publication number Publication date
WO2016065475A1 (fr) 2016-05-06
US20200030803A1 (en) 2020-01-30
CA2966007C (fr) 2019-10-22
CN107209170A (zh) 2017-09-26
EP3213067A4 (fr) 2018-05-30
US20190046984A1 (en) 2019-02-14
EP3213067A1 (fr) 2017-09-06
CA2966007A1 (fr) 2016-05-06
US10449542B2 (en) 2019-10-22
US10888864B2 (en) 2021-01-12
CA2993401A1 (fr) 2016-05-06

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