CA2993435C - Animaux resistants a des agents pathogenes ayant des genes cd163 modifies - Google Patents

Animaux resistants a des agents pathogenes ayant des genes cd163 modifies

Info

Publication number
CA2993435C
CA2993435C CA2993435A CA2993435A CA2993435C CA 2993435 C CA2993435 C CA 2993435C CA 2993435 A CA2993435 A CA 2993435A CA 2993435 A CA2993435 A CA 2993435A CA 2993435 C CA2993435 C CA 2993435C
Authority
CA
Canada
Prior art keywords
nucleotide
cell
base pair
deletion
sequence
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Active
Application number
CA2993435A
Other languages
English (en)
Other versions
CA2993435A1 (fr
Inventor
Randall S. Prather
Kevin D. Wells
Kristin M. Whitworth
Original Assignee
University of Missouri St Louis
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by University of Missouri St Louis filed Critical University of Missouri St Louis
Priority to CA3255239A priority Critical patent/CA3255239A1/en
Publication of CA2993435A1 publication Critical patent/CA2993435A1/fr
Application granted granted Critical
Publication of CA2993435C publication Critical patent/CA2993435C/fr
Active legal-status Critical Current
Anticipated expiration legal-status Critical

Links

Classifications

    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K67/00—Rearing or breeding animals, not otherwise provided for; New or modified breeds of animals
    • A01K67/027—New or modified breeds of vertebrates
    • A01K67/0275—Genetically modified vertebrates, e.g. transgenic
    • A01K67/0276—Knock-out vertebrates
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K67/00—Rearing or breeding animals, not otherwise provided for; New or modified breeds of animals
    • A01K67/027—New or modified breeds of vertebrates
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/705—Receptors; Cell surface antigens; Cell surface determinants
    • C07K14/70596—Molecules with a "CD"-designation not provided for elsewhere
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/113—Non-coding nucleic acids modulating the expression of genes, e.g. antisense oligonucleotides; Antisense DNA or RNA; Triplex- forming oligonucleotides; Catalytic nucleic acids, e.g. ribozymes; Nucleic acids used in co-suppression or gene silencing
    • C12N15/1138—Non-coding nucleic acids modulating the expression of genes, e.g. antisense oligonucleotides; Antisense DNA or RNA; Triplex- forming oligonucleotides; Catalytic nucleic acids, e.g. ribozymes; Nucleic acids used in co-suppression or gene silencing against receptors or cell surface proteins
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K2217/00—Genetically modified animals
    • A01K2217/07—Animals genetically altered by homologous recombination
    • A01K2217/075—Animals genetically altered by homologous recombination inducing loss of function, i.e. knock out
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K2227/00—Animals characterised by species
    • A01K2227/10—Mammal
    • A01K2227/108—Swine
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K2267/00—Animals characterised by purpose
    • A01K2267/02—Animal zootechnically ameliorated
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/113—Non-coding nucleic acids modulating the expression of genes, e.g. antisense oligonucleotides; Antisense DNA or RNA; Triplex- forming oligonucleotides; Catalytic nucleic acids, e.g. ribozymes; Nucleic acids used in co-suppression or gene silencing
    • C12N15/1131—Non-coding nucleic acids modulating the expression of genes, e.g. antisense oligonucleotides; Antisense DNA or RNA; Triplex- forming oligonucleotides; Catalytic nucleic acids, e.g. ribozymes; Nucleic acids used in co-suppression or gene silencing against viruses
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2310/00—Structure or type of the nucleic acid
    • C12N2310/10—Type of nucleic acid
    • C12N2310/20—Type of nucleic acid involving clustered regularly interspaced short palindromic repeats [CRISPR]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2770/00—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA ssRNA viruses positive-sense
    • C12N2770/00011—Details
    • C12N2770/10011—Arteriviridae

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Environmental Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Biotechnology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • Animal Behavior & Ethology (AREA)
  • Biodiversity & Conservation Biology (AREA)
  • Animal Husbandry (AREA)
  • General Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Biophysics (AREA)
  • Biochemistry (AREA)
  • Veterinary Medicine (AREA)
  • Plant Pathology (AREA)
  • Microbiology (AREA)
  • Physics & Mathematics (AREA)
  • Cell Biology (AREA)
  • Immunology (AREA)
  • Toxicology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Medicinal Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Virology (AREA)
  • Medicines Containing Material From Animals Or Micro-Organisms (AREA)

Abstract

La présente invention concerne des animaux non humains et leur descendance, comprenant au moins une séquence chromosomique modifiée dans un gène codant pour une protéine CD163. Des cellules animales qui contiennent de telles séquences chromosomiques modifiées sont également décrites. Les animaux et les cellules présentent une résistance accrue à des agents pathogènes, y compris le virus du syndrome reproducteur et respiratoire porcin (PRRSV). Les animaux et leur descendance présentent des modifications chromosomiques d'un gène CD163. L'invention concerne en outre des procédés d'amélioration génétique permettant de créer des animaux et des populations d'animaux résistants à des agents pathogènes produits au moyen de tels procédés.
CA2993435A 2015-08-06 2015-08-06 Animaux resistants a des agents pathogenes ayant des genes cd163 modifies Active CA2993435C (fr)

Priority Applications (1)

Application Number Priority Date Filing Date Title
CA3255239A CA3255239A1 (en) 2015-08-06 2015-08-06 Pathogen-resistant animals having modified cd163 genes

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
PCT/US2015/044113 WO2017023337A1 (fr) 2015-08-06 2015-08-06 Animaux résistants à des agents pathogènes ayant des gènes cd163 modifiés

Related Child Applications (1)

Application Number Title Priority Date Filing Date
CA3255239A Division CA3255239A1 (en) 2015-08-06 2015-08-06 Pathogen-resistant animals having modified cd163 genes

Publications (2)

Publication Number Publication Date
CA2993435A1 CA2993435A1 (fr) 2017-02-09
CA2993435C true CA2993435C (fr) 2025-09-09

Family

ID=57943379

Family Applications (2)

Application Number Title Priority Date Filing Date
CA3255239A Pending CA3255239A1 (en) 2015-08-06 2015-08-06 Pathogen-resistant animals having modified cd163 genes
CA2993435A Active CA2993435C (fr) 2015-08-06 2015-08-06 Animaux resistants a des agents pathogenes ayant des genes cd163 modifies

Family Applications Before (1)

Application Number Title Priority Date Filing Date
CA3255239A Pending CA3255239A1 (en) 2015-08-06 2015-08-06 Pathogen-resistant animals having modified cd163 genes

Country Status (4)

Country Link
KR (4) KR102495662B1 (fr)
AU (3) AU2015404563B2 (fr)
CA (2) CA3255239A1 (fr)
WO (1) WO2017023337A1 (fr)

Families Citing this family (10)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CA3255239A1 (en) * 2015-08-06 2026-03-02 The Curators Of The University Of Missouri Pathogen-resistant animals having modified cd163 genes
RS65691B1 (sr) * 2015-08-06 2024-07-31 Univ Missouri Svinja i ćelije rezistentne na virus reproduktivnog i respiratornog sindroma svinja (prrsv) koje imaju modifikovane cd163 gene
GB201617559D0 (en) * 2016-10-17 2016-11-30 University Court Of The University Of Edinburgh The Swine comprising modified cd163 and associated methods
WO2019090237A1 (fr) * 2017-11-03 2019-05-09 Genus Plc Éditions d'adn sélectivement ciblées chez le bétail
KR102680060B1 (ko) * 2018-04-17 2024-07-02 더 큐레이터스 오브 더 유니버시티 오브 미주리 바이러스에 의한 감염으로부터 돼지 태아를 보호하는 방법
US11160260B2 (en) 2018-04-17 2021-11-02 The Curators Of The University Of Missouri Methods for protecting porcine fetuses from infection with porcine reproductive and respiratory syndrome virus (PRRSV)
CN108753832A (zh) * 2018-04-20 2018-11-06 中山大学 一种利用CRISPR/Cas9编辑大白猪CD163基因的方法
CN108823248A (zh) * 2018-04-20 2018-11-16 中山大学 一种利用CRISPR/Cas9编辑陆川猪CD163基因的方法
MX2020010926A (es) * 2018-04-27 2021-01-08 Univ Missouri Animales resistentes a patogenos que tienen genes aminopeptidasa n (anpep) modificados.
CA3179710A1 (fr) * 2020-05-05 2021-11-11 Andrew Mark CIGAN Procedes pour l'amelioration de la sante d'especes porcines par inactivation ciblee de cd163

Family Cites Families (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US7196243B2 (en) * 2002-04-03 2007-03-27 Trillium Therapeutics Inc. Transgenic animal containing CD200 and uses therefor
US20110016546A1 (en) * 2008-12-04 2011-01-20 Sigma-Aldrich Co. Porcine genome editing with zinc finger nucleases
PT2709445T (pt) 2011-05-16 2018-11-27 Univ Missouri Animais resistentes ao vírus da síntese reprodutiva e respiratória porcino
CA2872789C (fr) * 2012-05-17 2019-09-03 Zoetis Llc Vaccination efficace contre le virus du syndrome reproducteur et respiratoire porcin (srrp) avant le sevrage
GB201313235D0 (en) * 2013-07-24 2013-09-04 Univ Edinburgh Antiviral Compositions Methods and Animals
CA3255239A1 (en) * 2015-08-06 2026-03-02 The Curators Of The University Of Missouri Pathogen-resistant animals having modified cd163 genes

Also Published As

Publication number Publication date
KR20180033548A (ko) 2018-04-03
AU2026200102A1 (en) 2026-01-29
KR102631856B1 (ko) 2024-02-01
KR20230021174A (ko) 2023-02-13
AU2023200913B2 (en) 2025-10-09
KR20240017121A (ko) 2024-02-06
AU2015404563A1 (en) 2018-02-15
WO2017023337A1 (fr) 2017-02-09
CA3255239A1 (en) 2026-03-02
KR20250016515A (ko) 2025-02-03
AU2015404563B2 (en) 2022-11-17
KR102760488B1 (ko) 2025-02-03
AU2023200913A1 (en) 2023-04-27
KR102495662B1 (ko) 2023-02-06
CA2993435A1 (fr) 2017-02-09

Similar Documents

Publication Publication Date Title
US12225889B2 (en) Pathogen-resistant animals having modified CD163 genes
AU2023200913B2 (en) Pathogen-resistant animals having modified cd163 genes
US12433263B2 (en) Methods for protecting porcine fetuses from infection with porcine reproductive and respiratory syndrome virus (PRRSV)
EP3169778B1 (fr) Knock-out nanos qui permet de pratiquer l'ablation des cellules de la lignée germinale
WO2019203807A1 (fr) Procédés de protection de fœtus porcins contre une infection par un virus
HK40105681A (en) Pathogen-resistant animals having modified cd163 genes
HK1256742B (en) Porcine reproductive and respiratory syndrome virus (prrsv)-resistant porcine and cells having modified cd163 genes

Legal Events

Date Code Title Description
EEER Examination request

Effective date: 20200728

MFA Maintenance fee for application paid

Free format text: FEE DESCRIPTION TEXT: MF (APPLICATION, 9TH ANNIV.) - STANDARD

Year of fee payment: 9

U00 Fee paid

Free format text: ST27 STATUS EVENT CODE: A-2-2-U10-U00-U101 (AS PROVIDED BY THE NATIONAL OFFICE); EVENT TEXT: MAINTENANCE REQUEST RECEIVED

Effective date: 20240705

D00 Search and/or examination requested or commenced

Free format text: ST27 STATUS EVENT CODE: A-2-2-D10-D00-D164 (AS PROVIDED BY THE NATIONAL OFFICE); EVENT TEXT: RESPONSE TO NOTICE OF ALLOWANCE

Effective date: 20241015

U11 Full renewal or maintenance fee paid

Free format text: ST27 STATUS EVENT CODE: A-2-2-U10-U11-U102 (AS PROVIDED BY THE NATIONAL OFFICE); EVENT TEXT: MAINTENANCE FEE PAYMENT DETERMINED COMPLIANT

Effective date: 20250123

Free format text: ST27 STATUS EVENT CODE: A-2-2-U10-U11-U102 (AS PROVIDED BY THE NATIONAL OFFICE); EVENT TEXT: MAINTENANCE FEE PAYMENT PAID IN FULL

Effective date: 20250123

D22 Grant of ip right intended

Free format text: ST27 STATUS EVENT CODE: A-2-4-D10-D22-D143 (AS PROVIDED BY THE NATIONAL OFFICE); EVENT TEXT: PRE-GRANT

Effective date: 20250616

W00 Other event occurred

Free format text: ST27 STATUS EVENT CODE: A-2-2-W10-W00-W111 (AS PROVIDED BY THE NATIONAL OFFICE); EVENT TEXT: CORRESPONDENT DETERMINED COMPLIANT

Effective date: 20250616

MFA Maintenance fee for application paid

Free format text: FEE DESCRIPTION TEXT: MF (APPLICATION, 10TH ANNIV.) - STANDARD

Year of fee payment: 10

U00 Fee paid

Free format text: ST27 STATUS EVENT CODE: A-4-4-U10-U00-U101 (AS PROVIDED BY THE NATIONAL OFFICE); EVENT TEXT: MAINTENANCE REQUEST RECEIVED

Effective date: 20250702

U11 Full renewal or maintenance fee paid

Free format text: ST27 STATUS EVENT CODE: A-4-4-U10-U11-U102 (AS PROVIDED BY THE NATIONAL OFFICE); EVENT TEXT: MAINTENANCE FEE PAYMENT PAID IN FULL

Effective date: 20250702

Q17 Modified document published

Free format text: ST27 STATUS EVENT CODE: A-4-4-Q10-Q17-Q103 (AS PROVIDED BY THE NATIONAL OFFICE); EVENT TEXT: DOCUMENT PUBLISHED

Effective date: 20250905

F11 Ip right granted following substantive examination

Free format text: ST27 STATUS EVENT CODE: A-4-4-F10-F11-X000 (AS PROVIDED BY THE NATIONAL OFFICE); EVENT TEXT: GRANT BY ISSUANCE

Effective date: 20250909