CA2997906C - Procede a base de gouttelettes et appareil pour l'analyse composite d'acide nucleique de cellules uniques - Google Patents

Procede a base de gouttelettes et appareil pour l'analyse composite d'acide nucleique de cellules uniques

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Publication number
CA2997906C
CA2997906C CA2997906A CA2997906A CA2997906C CA 2997906 C CA2997906 C CA 2997906C CA 2997906 A CA2997906 A CA 2997906A CA 2997906 A CA2997906 A CA 2997906A CA 2997906 C CA2997906 C CA 2997906C
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CA
Canada
Prior art keywords
cell
cells
rna
capture
sequence
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Active
Application number
CA2997906A
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English (en)
Other versions
CA2997906A1 (fr
Inventor
Aviv Regev
Evan Zane MACOSKO
Steven Andrew McCarroll
Alexander K. Shalek
Anindita BASU
Christopher B. FORD
Hongkun Park
David A. Weitz
Original Assignee
Massachusetts Institute of Technology
Broad Institute Inc
Harvard University
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Application filed by Massachusetts Institute of Technology, Broad Institute Inc, Harvard University filed Critical Massachusetts Institute of Technology
Priority claimed from PCT/US2015/049178 external-priority patent/WO2016040476A1/fr
Publication of CA2997906A1 publication Critical patent/CA2997906A1/fr
Application granted granted Critical
Publication of CA2997906C publication Critical patent/CA2997906C/fr
Active legal-status Critical Current
Anticipated expiration legal-status Critical

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Abstract

La présente invention concerne de manière générale une combinaison de marquage par code-barres moléculaire et de techniques microfluidiques à base d'émulsion pour isoler, lyser, marquer par code-barres, et préparer des acides nucléiques à partir de cellules individuelles selon un processus à haut débit.
CA2997906A 2014-09-09 2015-09-09 Procede a base de gouttelettes et appareil pour l'analyse composite d'acide nucleique de cellules uniques Active CA2997906C (fr)

Applications Claiming Priority (5)

Application Number Priority Date Filing Date Title
US201462048227P 2014-09-09 2014-09-09
US62/048,227 2014-09-09
US201562146642P 2015-04-13 2015-04-13
US62/146,642 2015-04-13
PCT/US2015/049178 WO2016040476A1 (fr) 2014-09-09 2015-09-09 Procédé à base de gouttelettes et appareil pour l'analyse composite d'acide nucléique de cellules uniques

Publications (2)

Publication Number Publication Date
CA2997906A1 CA2997906A1 (fr) 2016-03-17
CA2997906C true CA2997906C (fr) 2025-10-07

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