CA3013971A1 - Plateforme concue pour des tissus musculaires microstructures connectes - Google Patents

Plateforme concue pour des tissus musculaires microstructures connectes Download PDF

Info

Publication number: CA3013971A1
Authority: CA; Canada
Prior art keywords: protein; cell; micropattern; bridge; lanes
Prior art date: 2018-08-10
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.): Pending

Application number

CA3013971A

Other languages

English (en)

Inventor

Brett Nicholas Napiwocki

Max R. Salick

Wendy Catherine Crone

Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)

Wisconsin Alumni Research Foundation

Original Assignee

Wisconsin Alumni Research Foundation

Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)

2018-08-10

Filing date

2018-08-10

Publication date

2020-02-10

2018-08-10 Application filed by Wisconsin Alumni Research Foundation filed Critical Wisconsin Alumni Research Foundation

2018-08-10 Priority to CA3013971A priority Critical patent/CA3013971A1/fr

2020-02-10 Publication of CA3013971A1 publication Critical patent/CA3013971A1/fr

Status Pending legal-status Critical Current

Links

210000003205 muscle Anatomy 0.000 title abstract description 7
102000004169 proteins and genes Human genes 0.000 claims abstract description 187
108090000623 proteins and genes Proteins 0.000 claims abstract description 141
239000000758 substrate Substances 0.000 claims abstract description 59
210000004027 cell Anatomy 0.000 claims abstract description 54
238000000034 method Methods 0.000 claims abstract description 45
210000001519 tissue Anatomy 0.000 claims abstract description 36
210000000663 muscle cell Anatomy 0.000 claims abstract description 12
230000000638 stimulation Effects 0.000 claims description 13
230000021164 cell adhesion Effects 0.000 claims description 12
229920000642 polymer Polymers 0.000 claims description 10
102000010834 Extracellular Matrix Proteins Human genes 0.000 claims description 9
108010037362 Extracellular Matrix Proteins Proteins 0.000 claims description 9
210000002889 endothelial cell Anatomy 0.000 claims description 6
210000002950 fibroblast Anatomy 0.000 claims description 6
210000001778 pluripotent stem cell Anatomy 0.000 claims description 6
210000000130 stem cell Anatomy 0.000 claims description 6
239000003795 chemical substances by application Substances 0.000 claims description 5
238000012258 culturing Methods 0.000 claims description 5
239000011521 glass Substances 0.000 claims description 5
210000003061 neural cell Anatomy 0.000 claims description 5
229920003023 plastic Polymers 0.000 claims description 5
239000004033 plastic Substances 0.000 claims description 5
238000004113 cell culture Methods 0.000 claims description 4
239000010432 diamond Substances 0.000 claims description 3
238000010899 nucleation Methods 0.000 claims description 3
229910003460 diamond Inorganic materials 0.000 claims description 2
210000004165 myocardium Anatomy 0.000 abstract description 7
210000002027 skeletal muscle Anatomy 0.000 abstract description 5
239000000203 mixture Substances 0.000 abstract description 3
230000000144 pharmacologic effect Effects 0.000 abstract description 2
210000004413 cardiac myocyte Anatomy 0.000 description 23
-1 polydimethylsiloxane Polymers 0.000 description 11
238000000151 deposition Methods 0.000 description 9
239000000463 material Substances 0.000 description 9
230000008602 contraction Effects 0.000 description 8
239000004205 dimethyl polysiloxane Substances 0.000 description 7
229920000435 poly(dimethylsiloxane) Polymers 0.000 description 7
210000000107 myocyte Anatomy 0.000 description 6
210000003365 myofibril Anatomy 0.000 description 6
JWZZKOKVBUJMES-UHFFFAOYSA-N (+-)-Isoprenaline Chemical compound CC(C)NCC(O)C1=CC=C(O)C(O)=C1 JWZZKOKVBUJMES-UHFFFAOYSA-N 0.000 description 5
229940039009 isoproterenol Drugs 0.000 description 5
230000035800 maturation Effects 0.000 description 5
238000000813 microcontact printing Methods 0.000 description 5
230000008520 organization Effects 0.000 description 5
210000002235 sarcomere Anatomy 0.000 description 5
229920000954 Polyglycolide Polymers 0.000 description 4
230000000747 cardiac effect Effects 0.000 description 4
238000013461 design Methods 0.000 description 4
210000001671 embryonic stem cell Anatomy 0.000 description 4
210000004263 induced pluripotent stem cell Anatomy 0.000 description 4
230000001114 myogenic effect Effects 0.000 description 4
108090000765 processed proteins & peptides Proteins 0.000 description 4
241000870659 Crassula perfoliata var. minor Species 0.000 description 3
239000004372 Polyvinyl alcohol Substances 0.000 description 3
230000015556 catabolic process Effects 0.000 description 3
238000006731 degradation reaction Methods 0.000 description 3
201000010099 disease Diseases 0.000 description 3
208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 3
238000006073 displacement reaction Methods 0.000 description 3
239000003102 growth factor Substances 0.000 description 3
238000001727 in vivo Methods 0.000 description 3
238000010348 incorporation Methods 0.000 description 3
239000010410 layer Substances 0.000 description 3
238000001000 micrograph Methods 0.000 description 3
229920001610 polycaprolactone Polymers 0.000 description 3
239000004633 polyglycolic acid Substances 0.000 description 3
229920000307 polymer substrate Polymers 0.000 description 3
229920002451 polyvinyl alcohol Polymers 0.000 description 3
102000004196 processed proteins & peptides Human genes 0.000 description 3
230000001360 synchronised effect Effects 0.000 description 3
FWBHETKCLVMNFS-UHFFFAOYSA-N 4',6-Diamino-2-phenylindol Chemical compound C1=CC(C(=N)N)=CC=C1C1=CC2=CC=C(C(N)=N)C=C2N1 FWBHETKCLVMNFS-UHFFFAOYSA-N 0.000 description 2
IYMAXBFPHPZYIK-BQBZGAKWSA-N Arg-Gly-Asp Chemical compound NC(N)=NCCC[C@H](N)C(=O)NCC(=O)N[C@@H](CC(O)=O)C(O)=O IYMAXBFPHPZYIK-BQBZGAKWSA-N 0.000 description 2
208000024172 Cardiovascular disease Diseases 0.000 description 2
229920001661 Chitosan Polymers 0.000 description 2
102000008186 Collagen Human genes 0.000 description 2
108010035532 Collagen Proteins 0.000 description 2
102000016942 Elastin Human genes 0.000 description 2
108010014258 Elastin Proteins 0.000 description 2
108010067306 Fibronectins Proteins 0.000 description 2
102000016359 Fibronectins Human genes 0.000 description 2
102000007547 Laminin Human genes 0.000 description 2
108010085895 Laminin Proteins 0.000 description 2
238000010162 Tukey test Methods 0.000 description 2
238000004458 analytical method Methods 0.000 description 2
QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 description 2
230000008901 benefit Effects 0.000 description 2
229920001436 collagen Polymers 0.000 description 2
230000003436 cytoskeletal effect Effects 0.000 description 2
230000034994 death Effects 0.000 description 2
231100000517 death Toxicity 0.000 description 2
230000004069 differentiation Effects 0.000 description 2
229920002549 elastin Polymers 0.000 description 2
229920005570 flexible polymer Polymers 0.000 description 2
210000005003 heart tissue Anatomy 0.000 description 2
230000006698 induction Effects 0.000 description 2
238000000386 microscopy Methods 0.000 description 2
230000003278 mimic effect Effects 0.000 description 2
210000003098 myoblast Anatomy 0.000 description 2
238000001543 one-way ANOVA Methods 0.000 description 2
239000001301 oxygen Substances 0.000 description 2
229910052760 oxygen Inorganic materials 0.000 description 2
238000009832 plasma treatment Methods 0.000 description 2
229920000747 poly(lactic acid) Polymers 0.000 description 2
229920001606 poly(lactic acid-co-glycolic acid) Polymers 0.000 description 2
239000004632 polycaprolactone Substances 0.000 description 2
239000004626 polylactic acid Substances 0.000 description 2
229920002635 polyurethane Polymers 0.000 description 2
239000004814 polyurethane Substances 0.000 description 2
230000002035 prolonged effect Effects 0.000 description 2
210000002363 skeletal muscle cell Anatomy 0.000 description 2
208000013363 skeletal muscle disease Diseases 0.000 description 2
150000003384 small molecules Chemical class 0.000 description 2
230000004083 survival effect Effects 0.000 description 2
238000012546 transfer Methods 0.000 description 2
238000011282 treatment Methods 0.000 description 2
KIUKXJAPPMFGSW-DNGZLQJQSA-N (2S,3S,4S,5R,6R)-6-[(2S,3R,4R,5S,6R)-3-Acetamido-2-[(2S,3S,4R,5R,6R)-6-[(2R,3R,4R,5S,6R)-3-acetamido-2,5-dihydroxy-6-(hydroxymethyl)oxan-4-yl]oxy-2-carboxy-4,5-dihydroxyoxan-3-yl]oxy-5-hydroxy-6-(hydroxymethyl)oxan-4-yl]oxy-3,4,5-trihydroxyoxane-2-carboxylic acid Chemical compound CC(=O)N[C@H]1[C@H](O)O[C@H](CO)[C@@H](O)[C@@H]1O[C@H]1[C@H](O)[C@@H](O)[C@H](O[C@H]2[C@@H]([C@@H](O[C@H]3[C@@H]([C@@H](O)[C@H](O)[C@H](O3)C(O)=O)O)[C@H](O)[C@@H](CO)O2)NC(C)=O)[C@@H](C(O)=O)O1 KIUKXJAPPMFGSW-DNGZLQJQSA-N 0.000 description 1
102000010825 Actinin Human genes 0.000 description 1
108010063503 Actinin Proteins 0.000 description 1
102000007469 Actins Human genes 0.000 description 1
108010085238 Actins Proteins 0.000 description 1
102100024505 Bone morphogenetic protein 4 Human genes 0.000 description 1
102000000905 Cadherin Human genes 0.000 description 1
108050007957 Cadherin Proteins 0.000 description 1
OYPRJOBELJOOCE-UHFFFAOYSA-N Calcium Chemical compound [Ca] OYPRJOBELJOOCE-UHFFFAOYSA-N 0.000 description 1
108010067225 Cell Adhesion Molecules Proteins 0.000 description 1
102000016289 Cell Adhesion Molecules Human genes 0.000 description 1
239000004593 Epoxy Substances 0.000 description 1
AEMRFAOFKBGASW-UHFFFAOYSA-N Glycolic acid Polymers OCC(O)=O AEMRFAOFKBGASW-UHFFFAOYSA-N 0.000 description 1
229920002971 Heparan sulfate Polymers 0.000 description 1
HTTJABKRGRZYRN-UHFFFAOYSA-N Heparin Chemical compound OC1C(NC(=O)C)C(O)OC(COS(O)(=O)=O)C1OC1C(OS(O)(=O)=O)C(O)C(OC2C(C(OS(O)(=O)=O)C(OC3C(C(O)C(O)C(O3)C(O)=O)OS(O)(=O)=O)C(CO)O2)NS(O)(=O)=O)C(C(O)=O)O1 HTTJABKRGRZYRN-UHFFFAOYSA-N 0.000 description 1
244000043261 Hevea brasiliensis Species 0.000 description 1
101000762379 Homo sapiens Bone morphogenetic protein 4 Proteins 0.000 description 1
101001023043 Homo sapiens Myoblast determination protein 1 Proteins 0.000 description 1
101000613490 Homo sapiens Paired box protein Pax-3 Proteins 0.000 description 1
101000601661 Homo sapiens Paired box protein Pax-7 Proteins 0.000 description 1
108060003951 Immunoglobulin Proteins 0.000 description 1
208000026350 Inborn Genetic disease Diseases 0.000 description 1
JVTAAEKCZFNVCJ-UHFFFAOYSA-M Lactate Chemical compound CC(O)C([O-])=O JVTAAEKCZFNVCJ-UHFFFAOYSA-M 0.000 description 1
102100035077 Myoblast determination protein 1 Human genes 0.000 description 1
102000005604 Myosin Heavy Chains Human genes 0.000 description 1
108010084498 Myosin Heavy Chains Proteins 0.000 description 1
229920000459 Nitrile rubber Polymers 0.000 description 1
CBENFWSGALASAD-UHFFFAOYSA-N Ozone Chemical compound [O-][O+]=O CBENFWSGALASAD-UHFFFAOYSA-N 0.000 description 1
102100040891 Paired box protein Pax-3 Human genes 0.000 description 1
102100037503 Paired box protein Pax-7 Human genes 0.000 description 1
239000004952 Polyamide Substances 0.000 description 1
239000004642 Polyimide Substances 0.000 description 1
108010029485 Protein Isoforms Proteins 0.000 description 1
102000001708 Protein Isoforms Human genes 0.000 description 1
102000016611 Proteoglycans Human genes 0.000 description 1
108010067787 Proteoglycans Proteins 0.000 description 1
229920002472 Starch Polymers 0.000 description 1
239000004433 Thermoplastic polyurethane Substances 0.000 description 1
239000002253 acid Substances 0.000 description 1
230000036982 action potential Effects 0.000 description 1
108010023082 activin A Proteins 0.000 description 1
239000000853 adhesive Substances 0.000 description 1
230000001070 adhesive effect Effects 0.000 description 1
150000001413 amino acids Chemical group 0.000 description 1
238000013459 approach Methods 0.000 description 1
108010072041 arginyl-glycyl-aspartic acid Proteins 0.000 description 1
206010003119 arrhythmia Diseases 0.000 description 1
238000003556 assay Methods 0.000 description 1
239000011324 bead Substances 0.000 description 1
229960000074 biopharmaceutical Drugs 0.000 description 1
230000015572 biosynthetic process Effects 0.000 description 1
229910052791 calcium Inorganic materials 0.000 description 1
239000011575 calcium Substances 0.000 description 1
150000001720 carbohydrates Chemical class 0.000 description 1
235000014633 carbohydrates Nutrition 0.000 description 1
230000024245 cell differentiation Effects 0.000 description 1
230000010261 cell growth Effects 0.000 description 1
230000012292 cell migration Effects 0.000 description 1
239000013553 cell monolayer Substances 0.000 description 1
210000003850 cellular structure Anatomy 0.000 description 1
238000000576 coating method Methods 0.000 description 1
238000010276 construction Methods 0.000 description 1
229920001577 copolymer Polymers 0.000 description 1
125000004122 cyclic group Chemical group 0.000 description 1
229940079593 drug Drugs 0.000 description 1
239000003814 drug Substances 0.000 description 1
238000009509 drug development Methods 0.000 description 1
238000007876 drug discovery Methods 0.000 description 1
238000007877 drug screening Methods 0.000 description 1
238000007878 drug screening assay Methods 0.000 description 1
210000002242 embryoid body Anatomy 0.000 description 1
125000003700 epoxy group Chemical group 0.000 description 1
238000002474 experimental method Methods 0.000 description 1
210000002744 extracellular matrix Anatomy 0.000 description 1
125000000524 functional group Chemical group 0.000 description 1
208000016361 genetic disease Diseases 0.000 description 1
150000004676 glycans Chemical class 0.000 description 1
239000001963 growth medium Substances 0.000 description 1
230000036541 health Effects 0.000 description 1
208000019622 heart disease Diseases 0.000 description 1
229920002674 hyaluronan Polymers 0.000 description 1
229960003160 hyaluronic acid Drugs 0.000 description 1
230000005660 hydrophilic surface Effects 0.000 description 1
230000002209 hydrophobic effect Effects 0.000 description 1
230000005661 hydrophobic surface Effects 0.000 description 1
238000003384 imaging method Methods 0.000 description 1
102000018358 immunoglobulin Human genes 0.000 description 1
238000003364 immunohistochemistry Methods 0.000 description 1
230000006872 improvement Effects 0.000 description 1
238000000338 in vitro Methods 0.000 description 1
239000003112 inhibitor Substances 0.000 description 1
102000006495 integrins Human genes 0.000 description 1
108010044426 integrins Proteins 0.000 description 1
230000003993 interaction Effects 0.000 description 1
239000003446 ligand Substances 0.000 description 1
238000010859 live-cell imaging Methods 0.000 description 1
238000000691 measurement method Methods 0.000 description 1
230000005012 migration Effects 0.000 description 1
238000013508 migration Methods 0.000 description 1
238000002156 mixing Methods 0.000 description 1
238000012986 modification Methods 0.000 description 1
230000004048 modification Effects 0.000 description 1
208000010125 myocardial infarction Diseases 0.000 description 1
210000001087 myotubule Anatomy 0.000 description 1
229920003052 natural elastomer Polymers 0.000 description 1
229920001194 natural rubber Polymers 0.000 description 1
210000002569 neuron Anatomy 0.000 description 1
108010008217 nidogen Proteins 0.000 description 1
230000037361 pathway Effects 0.000 description 1
230000006461 physiological response Effects 0.000 description 1
229920000052 poly(p-xylylene) Polymers 0.000 description 1
229920002647 polyamide Polymers 0.000 description 1
229920000647 polyepoxide Polymers 0.000 description 1
229920000728 polyester Polymers 0.000 description 1
229920001223 polyethylene glycol Polymers 0.000 description 1
229920002338 polyhydroxyethylmethacrylate Polymers 0.000 description 1
229920001721 polyimide Polymers 0.000 description 1
229920001282 polysaccharide Polymers 0.000 description 1
239000005017 polysaccharide Substances 0.000 description 1
229920003225 polyurethane elastomer Polymers 0.000 description 1
229920000036 polyvinylpyrrolidone Polymers 0.000 description 1
239000001267 polyvinylpyrrolidone Substances 0.000 description 1
235000013855 polyvinylpyrrolidone Nutrition 0.000 description 1
238000011002 quantification Methods 0.000 description 1
230000005855 radiation Effects 0.000 description 1
238000009877 rendering Methods 0.000 description 1
230000004044 response Effects 0.000 description 1
230000011664 signaling Effects 0.000 description 1
229920002379 silicone rubber Polymers 0.000 description 1
238000004088 simulation Methods 0.000 description 1
230000007480 spreading Effects 0.000 description 1
238000010561 standard procedure Methods 0.000 description 1
235000019698 starch Nutrition 0.000 description 1
238000007619 statistical method Methods 0.000 description 1
229920003048 styrene butadiene rubber Polymers 0.000 description 1
235000000346 sugar Nutrition 0.000 description 1
150000008163 sugars Chemical class 0.000 description 1
238000004381 surface treatment Methods 0.000 description 1
229920003051 synthetic elastomer Polymers 0.000 description 1
239000005061 synthetic rubber Substances 0.000 description 1
238000012360 testing method Methods 0.000 description 1
230000001225 therapeutic effect Effects 0.000 description 1
238000002560 therapeutic procedure Methods 0.000 description 1
229920006344 thermoplastic copolyester Polymers 0.000 description 1
229920002725 thermoplastic elastomer Polymers 0.000 description 1
229920006345 thermoplastic polyamide Polymers 0.000 description 1
229920002803 thermoplastic polyurethane Polymers 0.000 description 1
238000012876 topography Methods 0.000 description 1
238000002723 toxicity assay Methods 0.000 description 1
239000003053 toxin Substances 0.000 description 1
231100000765 toxin Toxicity 0.000 description 1
108700012359 toxins Proteins 0.000 description 1
230000001052 transient effect Effects 0.000 description 1
238000002054 transplantation Methods 0.000 description 1
239000011800 void material Substances 0.000 description 1

Classifications

- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
- C12N5/06—Animal cells or tissues; Human cells or tissues
- C12N5/0602—Vertebrate cells
- C12N5/0652—Cells of skeletal and connective tissues; Mesenchyme
- C12N5/0657—Cardiomyocytes; Heart cells
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M21/00—Bioreactors or fermenters specially adapted for specific uses
- C12M21/08—Bioreactors or fermenters specially adapted for specific uses for producing artificial tissue or for ex-vivo cultivation of tissue
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M25/00—Means for supporting, enclosing or fixing the microorganisms, e.g. immunocoatings
- C12M25/14—Scaffolds; Matrices
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K35/00—Medicinal preparations containing materials or reaction products thereof with undetermined constitution
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2506/00—Differentiation of animal cells from one lineage to another; Differentiation of pluripotent cells
- C12N2506/45—Differentiation of animal cells from one lineage to another; Differentiation of pluripotent cells from artificially induced pluripotent stem cells
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2533/00—Supports or coatings for cell culture, characterised by material
- C12N2533/90—Substrates of biological origin, e.g. extracellular matrix, decellularised tissue
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2535/00—Supports or coatings for cell culture characterised by topography
- C12N2535/10—Patterned coating

Landscapes

Health & Medical Sciences (AREA)
Engineering & Computer Science (AREA)
Life Sciences & Earth Sciences (AREA)
Zoology (AREA)
Chemical & Material Sciences (AREA)
Biomedical Technology (AREA)
Organic Chemistry (AREA)
Wood Science & Technology (AREA)
Bioinformatics & Cheminformatics (AREA)
Genetics & Genomics (AREA)
Biotechnology (AREA)
Biochemistry (AREA)
General Engineering & Computer Science (AREA)
General Health & Medical Sciences (AREA)
Microbiology (AREA)
Sustainable Development (AREA)
Immunology (AREA)
Molecular Biology (AREA)
Cardiology (AREA)
Rheumatology (AREA)
Cell Biology (AREA)
Micro-Organisms Or Cultivation Processes Thereof (AREA)

CA3013971A 2018-08-10 2018-08-10 Plateforme concue pour des tissus musculaires microstructures connectes Pending CA3013971A1 (fr)

Priority Applications (1)

Application Number	Priority Date	Filing Date	Title
CA3013971A CA3013971A1 (fr)	2018-08-10	2018-08-10	Plateforme concue pour des tissus musculaires microstructures connectes

Applications Claiming Priority (1)

Application Number	Priority Date	Filing Date	Title
CA3013971A CA3013971A1 (fr)	2018-08-10	2018-08-10	Plateforme concue pour des tissus musculaires microstructures connectes

Publications (1)

Publication Number	Publication Date
CA3013971A1 true CA3013971A1 (fr)	2020-02-10

Family

ID=69514018

Family Applications (1)

Application Number	Title	Priority Date	Filing Date
CA3013971A Pending CA3013971A1 (fr)	2018-08-10	2018-08-10	Plateforme concue pour des tissus musculaires microstructures connectes

Country Status (1)

Country	Link
CA (1)	CA3013971A1 (fr)

2018
- 2018-08-10 CA CA3013971A patent/CA3013971A1/fr active Pending

Publication	Publication Date	Title
Mueller et al.	2021	Effects of external stimulators on engineered skeletal muscle tissue maturation
JP7227211B2 (ja)	2023-02-21	人工三次元皮膚組織、そのアレイ、およびその製造方法
Deutsch et al.	2000	Fabrication of microtextured membranes for cardiac myocyte attachment and orientation
Choi et al.	2012	The alignment and fusion assembly of adipose-derived stem cells on mechanically patterned matrices
EP1939280B1 (fr)	2010-07-07	Construction cellulaire cultivée qui continent des sphéroïdes de cellules animales cultivées et leur utilisation
Tan et al.	2003	Microfluidic patterning of cells in extracellular matrix biopolymers: effects of channel size, cell type, and matrix composition on pattern integrity
Ostrovidov et al.	2014	Skeletal muscle tissue engineering: methods to form skeletal myotubes and their applications
Khademhosseini et al.	2004	Layer-by-layer deposition of hyaluronic acid and poly-L-lysine for patterned cell co-cultures
Chan et al.	2012	Multi-material bio-fabrication of hydrogel cantilevers and actuators with stereolithography
Ricotti et al.	2010	Adhesion and proliferation of skeletal muscle cells on single layer poly (lactic acid) ultra-thin films
EP2766473A1 (fr)	2014-08-20	Système microphysiologique in vitro pour une organisation tissulaire 3d à haut débit et fonction biologique
WO2002026939A2 (fr)	2002-04-04	Microfabrication de membranes destinees a la croissance de cellules
Shimizu et al.	2013	Evaluation systems of generated forces of skeletal muscle cell-based bio-actuators
US20250304896A1 (en)	2025-10-02	Native Extracellular Matrix-Derived Membrane Inserts for Organs-On-Chips, Multilayer Microfluidics Microdevices, Bioreactors, Tissue Culture Inserts, and Two-Dimensional and Three-Dimensional Cell Culture Systems
Tsimbouri et al.	2014	Cell–material interactions
US20200048611A1 (en)	2020-02-13	Engineered platform for connected micropatterned cardiac tissues
EP3359213B1 (fr)	2022-02-23	Plate-forme à haut rendement permettant la bio-impression de modules tissulaires
CA3013971A1 (fr)	2020-02-10	Plateforme concue pour des tissus musculaires microstructures connectes
Desai et al.	1999	Microtextured cell culture platforms: Biomimetic substrates for the growth of cardiac myocytes and fibroblasts
US20230087578A1 (en)	2023-03-23	Device and methods for engineering 3d complex tissues
Portone et al.	2019	Quasi-3D morphology and modulation of focal adhesions of human adult stem cells through combinatorial concave elastomeric surfaces with varied stiffness
US20240400962A1 (en)	2024-12-05	Anchored cell construct culture
Ahrens	2022	Programming Complex Alignment in Engineered Cardiac Tissue
Deutsch et al.	2000	Microfrabricated in-vitro cell culture systems for investigating cellular interactions: fabricating a model system for cardiac myocytes
Srivastava	2012	Stem cell differentiation on stretchable conducting substrates