CA3069934A1 - Enrichissement negatif-positif pour la detection d'acides nucleiques - Google Patents
Enrichissement negatif-positif pour la detection d'acides nucleiques Download PDFInfo
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- CA3069934A1 CA3069934A1 CA3069934A CA3069934A CA3069934A1 CA 3069934 A1 CA3069934 A1 CA 3069934A1 CA 3069934 A CA3069934 A CA 3069934A CA 3069934 A CA3069934 A CA 3069934A CA 3069934 A1 CA3069934 A1 CA 3069934A1
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- nucleic acid
- sample
- segment
- dna
- mutation
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Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6813—Hybridisation assays
- C12Q1/6827—Hybridisation assays for detection of mutation or polymorphism
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
Landscapes
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Life Sciences & Earth Sciences (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Microbiology (AREA)
- Biochemistry (AREA)
- Physics & Mathematics (AREA)
- Molecular Biology (AREA)
- Biotechnology (AREA)
- Biophysics (AREA)
- Analytical Chemistry (AREA)
- Immunology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Saccharide Compounds (AREA)
Abstract
L'invention concerne des procédés de détection d'une caractéristique d'intérêt dans un échantillon d'acide nucléique par enrichissement négatif et positif de l'échantillon visant à identifier les segments qui contiennent la caractéristique d'intérêt. L'enrichissement négatif peut comprendre la digestion des acides nucléiques qui ne contiennent pas les segments, et l'enrichissement positif peut comprendre la purification des segments. Ces procédés sont utiles pour le diagnostic d'éléments génétiques, p. ex., les éléments indicateurs de cancer.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CA3222142A CA3222142A1 (fr) | 2017-06-13 | 2018-06-13 | Enrichissement negatif-positif pour la detection d'acides nucleiques |
Applications Claiming Priority (11)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US201762519051P | 2017-06-13 | 2017-06-13 | |
| US62/519,051 | 2017-06-13 | ||
| US201762526091P | 2017-06-28 | 2017-06-28 | |
| US62/526,091 | 2017-06-28 | ||
| US201762568121P | 2017-10-04 | 2017-10-04 | |
| US62/568,121 | 2017-10-04 | ||
| US15/877,619 US10527608B2 (en) | 2017-06-13 | 2018-01-23 | Methods for rare event detection |
| US15/877,619 | 2018-01-23 | ||
| US201862656592P | 2018-04-12 | 2018-04-12 | |
| US62/656,592 | 2018-04-12 | ||
| PCT/US2018/037277 WO2018231945A1 (fr) | 2017-06-13 | 2018-06-13 | Enrichissement négatif-positif pour la détection d'acides nucléiques |
Related Child Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CA3222142A Division CA3222142A1 (fr) | 2017-06-13 | 2018-06-13 | Enrichissement negatif-positif pour la detection d'acides nucleiques |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CA3069934A1 true CA3069934A1 (fr) | 2018-12-20 |
Family
ID=64659281
Family Applications (2)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CA3069934A Abandoned CA3069934A1 (fr) | 2017-06-13 | 2018-06-13 | Enrichissement negatif-positif pour la detection d'acides nucleiques |
| CA3222142A Pending CA3222142A1 (fr) | 2017-06-13 | 2018-06-13 | Enrichissement negatif-positif pour la detection d'acides nucleiques |
Family Applications After (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CA3222142A Pending CA3222142A1 (fr) | 2017-06-13 | 2018-06-13 | Enrichissement negatif-positif pour la detection d'acides nucleiques |
Country Status (3)
| Country | Link |
|---|---|
| EP (1) | EP3638809A4 (fr) |
| CA (2) | CA3069934A1 (fr) |
| WO (1) | WO2018231945A1 (fr) |
Families Citing this family (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US10081829B1 (en) | 2017-06-13 | 2018-09-25 | Genetics Research, Llc | Detection of targeted sequence regions |
| EP3625356B1 (fr) | 2017-08-08 | 2021-05-19 | Depixus | Isolation et enrichissement in vitro d'acides nucléiques à l'aide de nucléases spécifiques à un site |
| US20210040537A1 (en) * | 2019-08-08 | 2021-02-11 | Genetics Research, Llc | Selective enrichment |
| AU2023364547A1 (en) | 2022-10-21 | 2025-04-10 | Watchmaker Genomics, Inc. | Methods and compositions for sequencing library normalization |
Family Cites Families (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CA2817990A1 (fr) * | 2009-12-23 | 2011-06-30 | Genetic Technologies Limited | Procedes d'enrichissement et de detection d'acides nucleiques foetaux |
| WO2015183025A1 (fr) * | 2014-05-28 | 2015-12-03 | 주식회사 툴젠 | Procédé permettant la détection sensible d'adn cible à l'aide d'une nucléase spécifique de cible |
| EP3633047B1 (fr) * | 2014-08-19 | 2022-12-28 | Pacific Biosciences of California, Inc. | Procédés de séquenage d' acides nucléiques basé sur un enrichissement d'acides nucléiques |
| US10421993B2 (en) * | 2015-02-11 | 2019-09-24 | Paragon Genomics, Inc. | Methods and compositions for reducing non-specific amplification products |
| WO2016134136A2 (fr) * | 2015-02-20 | 2016-08-25 | The Johns Hopkins University | Altérations génomiques dans la tumeur et circulation de patients atteints d'un cancer du pancréas |
| US10538758B2 (en) * | 2015-08-19 | 2020-01-21 | Arc Bio, Llc | Capture of nucleic acids using a nucleic acid-guided nuclease-based system |
| US11268144B2 (en) * | 2015-09-24 | 2022-03-08 | Sigma-Aldrich Co. Llc | Methods and reagents for molecular proximity detection using RNA-guided nucleic acid binding proteins |
| EP3625356B1 (fr) * | 2017-08-08 | 2021-05-19 | Depixus | Isolation et enrichissement in vitro d'acides nucléiques à l'aide de nucléases spécifiques à un site |
| CA3093846A1 (fr) * | 2018-03-15 | 2019-09-19 | Twinstrand Biosciences, Inc. | Procedes et reactifs pour l'enrichissement de materiau d'acide nucleique pour des applications de sequencage et d'autres interrogations de materiau d'acide nucleique |
-
2018
- 2018-06-13 CA CA3069934A patent/CA3069934A1/fr not_active Abandoned
- 2018-06-13 EP EP18817717.4A patent/EP3638809A4/fr not_active Withdrawn
- 2018-06-13 CA CA3222142A patent/CA3222142A1/fr active Pending
- 2018-06-13 WO PCT/US2018/037277 patent/WO2018231945A1/fr not_active Ceased
Also Published As
| Publication number | Publication date |
|---|---|
| EP3638809A4 (fr) | 2021-03-10 |
| EP3638809A1 (fr) | 2020-04-22 |
| CA3222142A1 (fr) | 2018-12-20 |
| WO2018231945A1 (fr) | 2018-12-20 |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| FZDE | Discontinued |
Effective date: 20231213 |