CA3073372C - Technologie de modification specifique de sequence cible a l'aide d'une reconnaissance de cible nucleotidique - Google Patents

Technologie de modification specifique de sequence cible a l'aide d'une reconnaissance de cible nucleotidique

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Publication number
CA3073372C
CA3073372C CA3073372A CA3073372A CA3073372C CA 3073372 C CA3073372 C CA 3073372C CA 3073372 A CA3073372 A CA 3073372A CA 3073372 A CA3073372 A CA 3073372A CA 3073372 C CA3073372 C CA 3073372C
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CA
Canada
Prior art keywords
sequence
nucleotide sequence
cas5d
target nucleotide
cas7d
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Active
Application number
CA3073372A
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English (en)
Other versions
CA3073372A1 (fr
Inventor
Keishi Osakabe
Yuriko Osakabe
Original Assignee
University of Tokushima NUC
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Publication date
Application filed by University of Tokushima NUC filed Critical University of Tokushima NUC
Priority claimed from PCT/JP2018/030607 external-priority patent/WO2019039417A1/fr
Publication of CA3073372A1 publication Critical patent/CA3073372A1/fr
Application granted granted Critical
Publication of CA3073372C publication Critical patent/CA3073372C/fr
Active legal-status Critical Current
Anticipated expiration legal-status Critical

Links

Abstract

L'invention concerne un procédé pour cibler une séquence nucléotidique cible. Le procédé comprend l'introduction, dans une cellule : (i) de protéines associées à CRISPR de type I-D Cas5d, Cas6d et Cas7d ou d'acides nucléiques codant pour ces protéines ; et (ii) d'un ARN guide qui comprend une séquence complémentaire de ladite séquence nucléotidique cible et une séquence répétitive commune dérivée du locus du gène CRISPR avant et après ladite séquence complémentaire ou d'un ADN codant pour ledit ARN guide.
CA3073372A 2017-08-21 2018-08-20 Technologie de modification specifique de sequence cible a l'aide d'une reconnaissance de cible nucleotidique Active CA3073372C (fr)

Applications Claiming Priority (5)

Application Number Priority Date Filing Date Title
JP2017-158876 2017-08-21
JP2017158876 2017-08-21
JP2017-236518 2017-12-08
JP2017236518 2017-12-08
PCT/JP2018/030607 WO2019039417A1 (fr) 2017-08-21 2018-08-20 Technologie de modification spécifique de séquence cible à l'aide d'une reconnaissance de cible nucléotidique

Publications (2)

Publication Number Publication Date
CA3073372A1 CA3073372A1 (fr) 2019-02-28
CA3073372C true CA3073372C (fr) 2025-11-25

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