CA3077390A1 - Methodes et compositions pour la detection d'acides nucleiques - Google Patents

Methodes et compositions pour la detection d'acides nucleiques

Info

Publication number
CA3077390A1
CA3077390A1 CA3077390A CA3077390A CA3077390A1 CA 3077390 A1 CA3077390 A1 CA 3077390A1 CA 3077390 A CA3077390 A CA 3077390A CA 3077390 A CA3077390 A CA 3077390A CA 3077390 A1 CA3077390 A1 CA 3077390A1
Authority
CA
Canada
Prior art keywords
methods
compositions
nucleic acid
nucleic acids
acid detection
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
Application number
CA3077390A
Other languages
English (en)
Inventor
Aditya Rajagopal
Christopher Macdonald
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Amygdala Neurosciences Inc
Original Assignee
Amygdala Neurosciences Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Amygdala Neurosciences Inc filed Critical Amygdala Neurosciences Inc
Publication of CA3077390A1 publication Critical patent/CA3077390A1/fr
Pending legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/70—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving virus or bacteriophage
    • C12Q1/701—Specific hybridization probes
    • C12Q1/702—Specific hybridization probes for retroviruses
    • C12Q1/703—Viruses associated with AIDS
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/686—Polymerase chain reaction [PCR]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
    • C12N9/22—Ribonucleases [RNase]; Deoxyribonucleases [DNase]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6806—Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6816—Hybridisation assays characterised by the detection means
    • C12Q1/6818—Hybridisation assays characterised by the detection means involving interaction of two or more labels, e.g. resonant energy transfer
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6816—Hybridisation assays characterised by the detection means
    • C12Q1/6823—Release of bound markers
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/70—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving virus or bacteriophage
    • C12Q1/701—Specific hybridization probes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/70—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving virus or bacteriophage
    • C12Q1/701—Specific hybridization probes
    • C12Q1/706—Specific hybridization probes for hepatitis
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2521/00—Reaction characterised by the enzymatic activity
    • C12Q2521/30—Phosphoric diester hydrolysing, i.e. nuclease
    • C12Q2521/327—RNAse, e.g. RNAseH

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Engineering & Computer Science (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Genetics & Genomics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Molecular Biology (AREA)
  • Immunology (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Analytical Chemistry (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Virology (AREA)
  • Medicinal Chemistry (AREA)
  • Biomedical Technology (AREA)
  • Communicable Diseases (AREA)
  • AIDS & HIV (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
CA3077390A 2017-10-16 2018-10-15 Methodes et compositions pour la detection d'acides nucleiques Pending CA3077390A1 (fr)

Applications Claiming Priority (5)

Application Number Priority Date Filing Date Title
US201762573046P 2017-10-16 2017-10-16
US62/573,046 2017-10-16
US15/892,245 US20190112636A1 (en) 2017-10-16 2018-02-08 Methods and compositions for nucleic acid detection
US15/892,245 2018-02-08
PCT/US2018/055927 WO2019079204A1 (fr) 2017-10-16 2018-10-15 Méthodes et compositions pour la détection d'acides nucléiques

Publications (1)

Publication Number Publication Date
CA3077390A1 true CA3077390A1 (fr) 2019-04-25

Family

ID=66096918

Family Applications (1)

Application Number Title Priority Date Filing Date
CA3077390A Pending CA3077390A1 (fr) 2017-10-16 2018-10-15 Methodes et compositions pour la detection d'acides nucleiques

Country Status (5)

Country Link
US (2) US20190112636A1 (fr)
EP (2) EP3697931A4 (fr)
CN (1) CN111492067A (fr)
CA (1) CA3077390A1 (fr)
WO (1) WO2019079204A1 (fr)

Families Citing this family (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN107326085B (zh) 2012-02-03 2022-05-03 加州理工学院 多路生化测定中信号的编码和解码
IN2015DN01609A (fr) 2012-08-03 2015-07-03 California Inst Of Techn
CN112424377A (zh) 2018-04-17 2021-02-26 克罗玛科德公司 用于多重分析的方法和系统
US12203129B2 (en) 2018-07-03 2025-01-21 ChromaCode, Inc. Formulations and signal encoding and decoding methods for massively multiplexed biochemical assays
WO2024259564A1 (fr) * 2023-06-19 2024-12-26 深圳华大智造科技股份有限公司 Procédé pour la construction d'une banque ciblée selon un procédé en une étape et son utilisation

Family Cites Families (15)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5882856A (en) * 1995-06-07 1999-03-16 Genzyme Corporation Universal primer sequence for multiplex DNA amplification
DE69838210T2 (de) * 1997-12-15 2008-05-15 Csl Behring Gmbh Markierter Primer, geeignet für die Detektion von Nukleinsäuren
US6379888B1 (en) * 1999-09-27 2002-04-30 Becton, Dickinson And Company Universal probes and methods for detection of nucleic acids
US20040022764A1 (en) * 2002-07-31 2004-02-05 Hanan Polansky Inhibition of microcompetition with a foreign polynucleotide as treatment of chronic disease
WO2008118998A2 (fr) * 2007-03-27 2008-10-02 Primera Biosystems Inc. Procédé de détection multiplex et de quantification d'acides nucléiques
US20120231473A1 (en) * 2009-11-24 2012-09-13 Catholic University Industry Academic Cooperation Foundation Flow cytometry method through the control of fluorescence intensities
KR20130101952A (ko) * 2012-02-02 2013-09-16 주식회사 씨젠 Ｐｔｏ 절단과 연장-의존적 혼성화를 이용한 타겟 핵산서열의 검출
IN2015DN01609A (fr) * 2012-08-03 2015-07-03 California Inst Of Techn
GB201218909D0 (en) * 2012-10-22 2012-12-05 Univ Singapore Assay for the parallel detection of biological material based on PCR
US11254977B2 (en) * 2013-03-12 2022-02-22 Life Technologies Corporation Universal reporter-based genotyping methods and materials
CN106148521B (zh) * 2014-08-28 2019-11-15 广州和实生物技术有限公司 一种多点突变单管快速检测方法及试剂盒
CN104404162A (zh) * 2014-12-16 2015-03-11 郑州安图生物工程股份有限公司 采用引物关联通用探针检测多重基因或不同靶标的实时荧光pcr方法
ES2961374T3 (es) * 2015-04-24 2024-03-11 Atila Biosystems Incorporated Amplificación con cebadores de composición de nucleótidos limitada
EP4219519A3 (fr) * 2016-04-01 2023-09-13 Chromacode, Inc. Sondes compétitives destinées à la production d'un signal d'ingénierie
CN106636409A (zh) * 2016-12-28 2017-05-10 健路生物科技（苏州）有限公司 一种通用荧光探针及其检测方法和应用

Also Published As

Publication number Publication date
US20210087607A1 (en) 2021-03-25
EP4083229A1 (fr) 2022-11-02
US20190112636A1 (en) 2019-04-18
WO2019079204A1 (fr) 2019-04-25
EP3697931A1 (fr) 2020-08-26
EP3697931A4 (fr) 2021-10-13
CN111492067A (zh) 2020-08-04

Similar Documents

Publication Publication Date Title
EP4303314A3 (fr) Nucléotides à marquage polypeptidique et leur utilisation dans le séquençage d'acide nucléique par détection par nanopores
WO2018107129A8 (fr) Diagnostics basés sur un système effecteur crispr
EP4306659A3 (fr) Procédé et kit pour déterminer l'origine tissulaire ou cellulaire d'adn
WO2015058008A3 (fr) Identification et détection d'acide nucléique améliorées
EP4379065A3 (fr) Mesures fluorimétriques multiplexées avec systèmes de pcr en gouttelettes
EP4647507A3 (fr) Procédé de détection d'acide nucléique
WO2016064894A3 (fr) Compositions et procédés pour la détection d'un virus à arn
SG10201805453XA (en) Multiplexed analysis of target nucleic acids
NZ701145A (en) Compositions and methods for quantifying a nucleic acid sequence in a sample
MX376755B (es) Detección de ácidos nucleicos diana mediante el uso de hibridación.
WO2015196130A3 (fr) Molécules d'acide nucléique alternatives et leurs utilisations
SG11201903066RA (en) Method for multiplex detection of methylated dna
GB201017978D0 (en) Multiplex amplification and detection
SG10201806000YA (en) Primer set for pcr for bacterial dna amplification, kit for detection and/or identification of bacterial species, and, method of detection and/or identification of bacterial species
BR112016029907A2 (pt) enriquecimento geneticamente adaptado e reação em cadeia da polimerase para a rápida detecção de microrganismos".
WO2016059473A3 (fr) Adn de conversion de séquence et amplificateur de signal comportant des acides nucléiques verrouillés et procédés de détection l'utilisant
WO2013134401A3 (fr) Compositions de nanocapteur enzymatique et procédés
EP4321867A3 (fr) Sondes pour discrimination et multiplexage améliorés de fusion dans des dosages d'acides nucléiques
WO2012064978A3 (fr) Compositions, procédés et kits pour détecter et identifier des mycobactéries
EP4282961A3 (fr) Compositions et procédés pour la détection et l'analyse de mycobacterium tuberculosis
GB2542576A (en) Method and kit of detecting the absence of micro-oranisms
WO2019239218A3 (fr) Détermination de modifications épigénétiques par séquençage de nanopores
AU2015215750A2 (en) Nucleic acid detection or quantification method using mask oligonucleotide, and device for same
EP4464412A3 (fr) Détection d'amplification par recombinase polymérase à l'aide d'une sonde à double hapten
AU2014306512A8 (en) Compositions and methods for detecting HEV nucleic acid