CA3100492A1 - Enrichissement et detection de methylation specifique a une sequence - Google Patents

Enrichissement et detection de methylation specifique a une sequence Download PDF

Info

Publication number
CA3100492A1
CA3100492A1 CA3100492A CA3100492A CA3100492A1 CA 3100492 A1 CA3100492 A1 CA 3100492A1 CA 3100492 A CA3100492 A CA 3100492A CA 3100492 A CA3100492 A CA 3100492A CA 3100492 A1 CA3100492 A1 CA 3100492A1
Authority
CA
Canada
Prior art keywords
sample
target
dna
target region
nucleic acid
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Abandoned
Application number
CA3100492A
Other languages
English (en)
Inventor
Anthony P. Shuber
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Genetics Research LLC
Original Assignee
Genetics Research LLC
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from US16/018,926 external-priority patent/US20190002964A1/en
Application filed by Genetics Research LLC filed Critical Genetics Research LLC
Publication of CA3100492A1 publication Critical patent/CA3100492A1/fr
Abandoned legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
    • C12N9/22—Ribonucleases [RNase]; Deoxyribonucleases [DNase]
    • C12N9/222—Clustered regularly interspaced short palindromic repeats [CRISPR]-associated [CAS] enzymes
    • C12N9/226—Class 2 CAS enzyme complex, e.g. single CAS protein
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
    • C12N9/22—Ribonucleases [RNase]; Deoxyribonucleases [DNase]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6804—Nucleic acid analysis using immunogens
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6827—Hybridisation assays for detection of mutation or polymorphism

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Genetics & Genomics (AREA)
  • Molecular Biology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • Immunology (AREA)
  • Analytical Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Medicinal Chemistry (AREA)
  • Biomedical Technology (AREA)
  • Pathology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

L'invention concerne des procédés permettant de détecter des changements épigénétiques, comprenant, mais sans s'y limiter, des changements de méthylation, directement à partir d'échantillons biologiques, sans avoir besoin de certaines étapes de préparation d'échantillon complexes. L'invention concerne des complexes de protéine Cas/ARN de guidage qui peuvent être introduits directement dans l'échantillon, où les complexes ciblent et se lient à la région cible. La région cible est ainsi enrichie et isolée d'une manière spécifique de séquence. La région cible peut ensuite être soumise à n'importe quel essai approprié d'amplification de signal pour détecter le changement épigénétique dans la région cible. La détection d'une hyperméthylation de l'ADN dans la région cible indique une maladie, telle qu'un cancer.
CA3100492A 2018-05-16 2018-10-03 Enrichissement et detection de methylation specifique a une sequence Abandoned CA3100492A1 (fr)

Applications Claiming Priority (5)

Application Number Priority Date Filing Date Title
US201862672217P 2018-05-16 2018-05-16
US62/672,217 2018-05-16
US16/018,926 US20190002964A1 (en) 2017-06-28 2018-06-26 Bodily fluid target enrichment
US16/018,926 2018-06-26
PCT/US2018/054188 WO2019221769A1 (fr) 2018-05-16 2018-10-03 Enrichissement et détection de méthylation spécifique à une séquence

Publications (1)

Publication Number Publication Date
CA3100492A1 true CA3100492A1 (fr) 2019-11-21

Family

ID=68540705

Family Applications (2)

Application Number Title Priority Date Filing Date
CA3100492A Abandoned CA3100492A1 (fr) 2018-05-16 2018-10-03 Enrichissement et detection de methylation specifique a une sequence
CA3069939A Abandoned CA3069939A1 (fr) 2018-05-16 2018-10-12 Detection et surveillance de tumeur

Family Applications After (1)

Application Number Title Priority Date Filing Date
CA3069939A Abandoned CA3069939A1 (fr) 2018-05-16 2018-10-12 Detection et surveillance de tumeur

Country Status (3)

Country Link
EP (2) EP3794139A4 (fr)
CA (2) CA3100492A1 (fr)
WO (2) WO2019221769A1 (fr)

Families Citing this family (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US10081829B1 (en) 2017-06-13 2018-09-25 Genetics Research, Llc Detection of targeted sequence regions
WO2021108366A1 (fr) * 2019-11-27 2021-06-03 Genetics Research, Llc Enrichissement de crispr d'allèle rare ciblé
JP2025522763A (ja) * 2022-06-30 2025-07-17 ガーダント ヘルス， インコーポレイテッド 異常にメチル化されたｄｎａの富化

Family Cites Families (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US9109256B2 (en) * 2004-10-27 2015-08-18 Esoterix Genetic Laboratories, Llc Method for monitoring disease progression or recurrence
US8916530B2 (en) * 2005-11-18 2014-12-23 Gradalis, Inc. Individualized cancer therapy
CA2955382C (fr) * 2014-07-21 2023-07-18 Illumina, Inc. Enrichissement de polynucleotides a l'aide de systemes crispr-cas
EP3633047B1 (fr) * 2014-08-19 2022-12-28 Pacific Biosciences of California, Inc. Procédés de séquenage d' acides nucléiques basé sur un enrichissement d'acides nucléiques
AU2016263026A1 (en) * 2015-05-15 2017-11-09 Pioneer Hi-Bred International, Inc. Guide RNA/Cas endonuclease systems
US20170014449A1 (en) * 2015-07-13 2017-01-19 Elwha LLC, a limited liability company of the State of Delaware Site-specific epigenetic editing
US10081829B1 (en) * 2017-06-13 2018-09-25 Genetics Research, Llc Detection of targeted sequence regions

Also Published As

Publication number Publication date
EP3655546A4 (fr) 2021-04-07
WO2019221773A1 (fr) 2019-11-21
EP3794139A1 (fr) 2021-03-24
WO2019221769A1 (fr) 2019-11-21
EP3794139A4 (fr) 2022-06-01
EP3655546A1 (fr) 2020-05-27
CA3069939A1 (fr) 2019-11-21

Similar Documents

Publication Publication Date Title
US20240200122A1 (en) Bodily fluid target enrichment
US20240309355A1 (en) Solid phase negative enrichment
US20190032116A1 (en) Sequence specific methylation enrichment and detection
EP2505666B1 (fr) Procédés et compositions pour l'enrichissement soit de polynucléotides cibles ou de polynucléotides non-cibles à partir d'un mélange de polynucléotides cibles et non-cibles
JP2025060959A (ja) 配列決定用途および他の核酸物質インテロゲーションのための核酸物質を濃縮するための方法および試薬
EP1169479B1 (fr) Procedes de detection d'acides nucleiques revelateurs de cancer
US20190187031A1 (en) Concentration of analytes
EP3885448A1 (fr) Analyse de la chromatine au moyen d'une enzyme de coupure
JP2002539765A (ja) 糞便サンプル調製のための方法
EP3481952A1 (fr) Marqueurs de méthylation dans le traitement de la leucémie et leurs utilisations
US20190048425A1 (en) Tumor detection and monitoring
US20190382824A1 (en) Negative-positive enrichment for nucleic acid detection
US20180355437A1 (en) Plasma/serum target enrichment
CA3100492A1 (fr) Enrichissement et detection de methylation specifique a une sequence
WO2018231945A1 (fr) Enrichissement négatif-positif pour la détection d'acides nucléiques
US20190071716A1 (en) Bodily fluid enrichment
US20250188511A1 (en) Contamination-free metagenomic dna sequencing
WO2020190613A1 (fr) Procédés de détection d'une maladie résiduelle minimale
WO2017090543A1 (fr) Procédé d'analyse d'interaction entre adn
CN121674542A (zh) 基于MeDIP-MSRE的全基因组甲基化检测方法
CN120400318A (zh) 一种单细胞CUT&Tag文库制备方法
CN120738328A (zh) 基于甲基化敏感限制性内切酶的文库污染特异性降解方法及试剂盒
WO2021055385A1 (fr) Dosage diagnostique pour le cancer

Legal Events

Date Code Title Description
FZDE Discontinued

Effective date: 20240403