CA3139169A1 - Procede de sequencage a canal unique base sur l'auto-luminescence - Google Patents

Procede de sequencage a canal unique base sur l'auto-luminescence Download PDF

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Publication number
CA3139169A1
CA3139169A1 CA3139169A CA3139169A CA3139169A1 CA 3139169 A1 CA3139169 A1 CA 3139169A1 CA 3139169 A CA3139169 A CA 3139169A CA 3139169 A CA3139169 A CA 3139169A CA 3139169 A1 CA3139169 A1 CA 3139169A1
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CA
Canada
Prior art keywords
molecular label
luciferase
linked
nucleic acid
nucleotide
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
Application number
CA3139169A
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English (en)
Inventor
Sha LIAO
Xi Chen
Ao CHEN
Wenwei Zhang
Chongjun Xu
Hongmin Chen
Jie Zhao
Defeng FU
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Qingdao MGI Tech Co Ltd
Original Assignee
Egi Tech Shenzhen Co Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Egi Tech Shenzhen Co Ltd filed Critical Egi Tech Shenzhen Co Ltd
Publication of CA3139169A1 publication Critical patent/CA3139169A1/fr
Pending legal-status Critical Current

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6869—Methods for sequencing
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6869—Methods for sequencing
    • C12Q1/6874—Methods for sequencing involving nucleic acid arrays, e.g. sequencing by hybridisation
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6853—Nucleic acid amplification reactions using modified primers or templates

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  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • Biotechnology (AREA)
  • Molecular Biology (AREA)
  • Biophysics (AREA)
  • Analytical Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Immunology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

La présente invention concerne un procédé de séquençage basé sur un seul colorant fluorescent, comprenant l'utilisation de signaux auto-lumineux pour distinguer l'incorporation séquentielle de différents nucléotides, ce qui permet de déterminer une séquence polynucléotidique.
CA3139169A 2019-05-15 2019-05-15 Procede de sequencage a canal unique base sur l'auto-luminescence Pending CA3139169A1 (fr)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
PCT/CN2019/086974 WO2020227953A1 (fr) 2019-05-15 2019-05-15 Procédé de séquençage à canal unique basé sur l'auto-luminescence

Publications (1)

Publication Number Publication Date
CA3139169A1 true CA3139169A1 (fr) 2020-11-19

Family

ID=73289989

Family Applications (1)

Application Number Title Priority Date Filing Date
CA3139169A Pending CA3139169A1 (fr) 2019-05-15 2019-05-15 Procede de sequencage a canal unique base sur l'auto-luminescence

Country Status (12)

Country Link
US (1) US12565677B2 (fr)
EP (1) EP3988670B1 (fr)
JP (2) JP7485483B2 (fr)
KR (1) KR102843262B1 (fr)
CN (2) CN118931711A (fr)
AU (1) AU2019445584B2 (fr)
BR (1) BR112021022809A2 (fr)
CA (1) CA3139169A1 (fr)
MX (1) MX2021013983A (fr)
SG (1) SG11202111778YA (fr)
WO (1) WO2020227953A1 (fr)
ZA (1) ZA202110159B (fr)

Families Citing this family (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN114286867B (zh) * 2019-08-20 2024-04-16 青岛华大智造科技有限责任公司 一种基于发光标记物光信号动力学及二次发光信号对多核苷酸进行测序的方法
IL315955A (en) 2022-03-30 2024-11-01 Peptidream Inc Peptide complex having trkb binding activity
CN115232867B (zh) * 2022-07-21 2024-01-30 深圳赛陆医疗科技有限公司 一种快速的二代基因测序方法
JP2025531816A (ja) 2022-09-09 2025-09-25 ビージーアイ シェンチェン 核酸検出におけるヘテロ芳香環化合物の使用
US20250297306A1 (en) 2022-09-28 2025-09-25 Mgi Tech Co., Ltd. Sequencing method
WO2024098185A1 (fr) * 2022-11-07 2024-05-16 GeneSense Technology Inc., Shanghai (CN) Séquençage d'acide nucléique à l'aide d'une auto-luminescence
EP4592403A4 (fr) 2022-11-22 2025-11-26 Mgi Tech Co Ltd Puce de séquençage de gènes, structure de conditionnement, système et procédés de nettoyage, de production et de séquençage

Family Cites Families (16)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1991006678A1 (fr) 1989-10-26 1991-05-16 Sri International Sequençage d'adn
WO2000006770A1 (fr) 1998-07-30 2000-02-10 Solexa Ltd. Biomolecules en rangees et leur utilisation dans une procedure de sequençage
AU2001296645A1 (en) 2000-10-06 2002-04-15 The Trustees Of Columbia University In The City Of New York Massive parallel method for decoding dna and rna
WO2004018497A2 (fr) 2002-08-23 2004-03-04 Solexa Limited Nucleotides modifies
EP1701785A1 (fr) 2004-01-07 2006-09-20 Solexa Ltd. Reseaux moleculaires modifies
GB0427236D0 (en) 2004-12-13 2005-01-12 Solexa Ltd Improved method of nucleotide detection
CN1854308A (zh) * 2005-04-21 2006-11-01 株式会社日立制作所 碱基序列检测方法
CA2859660C (fr) * 2011-09-23 2021-02-09 Illumina, Inc. Procedes et compositions de sequencage d'acides nucleiques
WO2014139596A1 (fr) 2013-03-15 2014-09-18 Illumina Cambridge Limited Nucléosides ou nucléotides modifiés
ES2803079T3 (es) * 2014-09-12 2021-01-22 Illumina Inc Métodos para detectar la presencia de subunidades de polímeros usando quimioluminiscencia
EP3411494B1 (fr) * 2015-11-18 2025-08-13 Kalim U. Mir Séquençage super-résolution
CN113321943A (zh) * 2015-11-20 2021-08-31 加利福尼亚太平洋生物科学股份有限公司 标记的核苷酸类似物、反应混合物以及测序方法和系统
RU2760737C2 (ru) 2016-12-27 2021-11-30 Еги Тек (Шэнь Чжэнь) Ко., Лимитед Способ секвенирования на основе одного флуоресцентного красителя
ES3026859T3 (en) 2017-01-04 2025-06-12 Mgi Tech Co Ltd Stepwise sequencing by non-labeled reversible terminators or natural nucleotides
BR112019014683A2 (pt) 2017-03-07 2020-02-18 Illumina, Inc. Sequenciamento com dois canais óticos, com fonte de luz única
WO2019071474A1 (fr) * 2017-10-11 2019-04-18 深圳华大智造科技有限公司 Nucléoside ou nucléotide modifié

Also Published As

Publication number Publication date
CN118931711A (zh) 2024-11-12
JP2022533916A (ja) 2022-07-27
EP3988670A1 (fr) 2022-04-27
CN113748216B (zh) 2024-04-23
SG11202111778YA (en) 2021-12-30
KR102843262B1 (ko) 2025-08-06
KR20220009992A (ko) 2022-01-25
AU2019445584B2 (en) 2024-09-05
JP2024099789A (ja) 2024-07-25
CN113748216A (zh) 2021-12-03
EP3988670A4 (fr) 2023-01-25
BR112021022809A2 (pt) 2022-01-25
MX2021013983A (es) 2022-02-10
JP7485483B2 (ja) 2024-05-16
EP3988670B1 (fr) 2026-04-22
JP7825660B2 (ja) 2026-03-06
AU2019445584A1 (en) 2022-01-20
US20220205036A1 (en) 2022-06-30
ZA202110159B (en) 2022-08-31
US12565677B2 (en) 2026-03-03
WO2020227953A1 (fr) 2020-11-19

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