CA3140900A1 - Sequencage par emergence - Google Patents

Sequencage par emergence Download PDF

Info

Publication number
CA3140900A1
CA3140900A1 CA3140900A CA3140900A CA3140900A1 CA 3140900 A1 CA3140900 A1 CA 3140900A1 CA 3140900 A CA3140900 A CA 3140900A CA 3140900 A CA3140900 A CA 3140900A CA 3140900 A1 CA3140900 A1 CA 3140900A1
Authority
CA
Canada
Prior art keywords
oligonucleotide probe
nucleic acid
probe species
species
target nucleic
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
Application number
CA3140900A
Other languages
English (en)
Inventor
Kalim Mir
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Xgenomes Corp
Original Assignee
Xgenomes Corp
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from US16/425,929 external-priority patent/US11427867B2/en
Application filed by Xgenomes Corp filed Critical Xgenomes Corp
Publication of CA3140900A1 publication Critical patent/CA3140900A1/fr
Pending legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6869—Methods for sequencing
    • C12Q1/6874—Methods for sequencing involving nucleic acid arrays, e.g. sequencing by hybridisation
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6816—Hybridisation assays characterised by the detection means
    • C12Q1/6818—Hybridisation assays characterised by the detection means involving interaction of two or more labels, e.g. resonant energy transfer
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6869—Methods for sequencing
    • G—PHYSICS
    • G11—INFORMATION STORAGE
    • G11C—STATIC STORES
    • G11C13/00—Digital stores characterised by the use of storage elements not covered by groups G11C11/00, G11C23/00, or G11C25/00
    • G11C13/0002—Digital stores characterised by the use of storage elements not covered by groups G11C11/00, G11C23/00, or G11C25/00 using resistive RAM [RRAM] elements
    • G11C13/0009—RRAM elements whose operation depends upon chemical change
    • G11C13/0014—RRAM elements whose operation depends upon chemical change comprising cells based on organic memory material
    • G11C13/0019—RRAM elements whose operation depends upon chemical change comprising cells based on organic memory material comprising bio-molecules
    • G—PHYSICS
    • G16—INFORMATION AND COMMUNICATION TECHNOLOGY [ICT] SPECIALLY ADAPTED FOR SPECIFIC APPLICATION FIELDS
    • G16B—BIOINFORMATICS, i.e. INFORMATION AND COMMUNICATION TECHNOLOGY [ICT] SPECIALLY ADAPTED FOR GENETIC OR PROTEIN-RELATED DATA PROCESSING IN COMPUTATIONAL MOLECULAR BIOLOGY
    • G16B20/00—ICT specially adapted for functional genomics or proteomics, e.g. genotype-phenotype associations
    • G16B20/30—Detection of binding sites or motifs
    • G—PHYSICS
    • G16—INFORMATION AND COMMUNICATION TECHNOLOGY [ICT] SPECIALLY ADAPTED FOR SPECIFIC APPLICATION FIELDS
    • G16B—BIOINFORMATICS, i.e. INFORMATION AND COMMUNICATION TECHNOLOGY [ICT] SPECIALLY ADAPTED FOR GENETIC OR PROTEIN-RELATED DATA PROCESSING IN COMPUTATIONAL MOLECULAR BIOLOGY
    • G16B25/00—ICT specially adapted for hybridisation; ICT specially adapted for gene or protein expression
    • G16B25/20—Polymerase chain reaction [PCR]; Primer or probe design; Probe optimisation
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2563/00—Nucleic acid detection characterized by the use of physical, structural and functional properties
    • C12Q2563/113—Nucleic acid detection characterized by the use of physical, structural and functional properties the label being electroactive, e.g. redox labels
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2600/00—Oligonucleotides characterized by their use
    • C12Q2600/156—Polymorphic or mutational markers

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Physics & Mathematics (AREA)
  • Organic Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biophysics (AREA)
  • Biotechnology (AREA)
  • Genetics & Genomics (AREA)
  • Molecular Biology (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • General Health & Medical Sciences (AREA)
  • Analytical Chemistry (AREA)
  • Spectroscopy & Molecular Physics (AREA)
  • General Engineering & Computer Science (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • Evolutionary Biology (AREA)
  • Medical Informatics (AREA)
  • Immunology (AREA)
  • Theoretical Computer Science (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

L'invention concerne un procédé de séquençage de polymères dans lequel la séquence d'un ou de plusieurs polymères est déterminée par l'intermédiaire d'une propriété émergente des interactions de liaison d'un répertoire de sondes moléculaires au(x) polymère(s).
CA3140900A 2019-05-29 2020-05-27 Sequencage par emergence Pending CA3140900A1 (fr)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US16/425,929 US11427867B2 (en) 2017-11-29 2019-05-29 Sequencing by emergence
US16/425,929 2019-05-29
PCT/US2020/034726 WO2020243187A1 (fr) 2019-05-29 2020-05-27 Séquençage par émergence

Publications (1)

Publication Number Publication Date
CA3140900A1 true CA3140900A1 (fr) 2020-12-03

Family

ID=73553066

Family Applications (1)

Application Number Title Priority Date Filing Date
CA3140900A Pending CA3140900A1 (fr) 2019-05-29 2020-05-27 Sequencage par emergence

Country Status (7)

Country Link
EP (1) EP3976828A4 (fr)
JP (1) JP2022534920A (fr)
KR (1) KR20220034054A (fr)
CN (1) CN114207149A (fr)
AU (1) AU2020282704A1 (fr)
CA (1) CA3140900A1 (fr)
WO (1) WO2020243187A1 (fr)

Families Citing this family (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US11486001B2 (en) * 2021-02-08 2022-11-01 Singular Genomics Systems, Inc. Methods and compositions for sequencing complementary polynucleotides
US20260078429A1 (en) * 2021-04-06 2026-03-19 Kalim Mir Systems, methods, and compositions for detecting epigenetic modifications of nucleic acids
EP4216105B1 (fr) * 2022-01-25 2025-03-12 Leica Microsystems CMS GmbH Marqueur et procédé d'analyse d'échantillons biologiques
EP4544077A1 (fr) * 2022-06-21 2025-04-30 Nautilus Subsidiary, Inc. Procédé pour la détection d'analytes sur des sites dont la distance n'est pas optiquement résolvable
US12347527B2 (en) 2022-08-23 2025-07-01 Leica Microsystems Cms Gmbh Data storage device and method for storing data using an oligonucleotide nanostructure backbone and labels with dyes
CN120019165A (zh) * 2023-03-01 2025-05-16 深圳华大智造科技股份有限公司 确定测序中反应时间的方法和测序方法及系统
CN116596933B (zh) * 2023-07-18 2023-09-29 深圳赛陆医疗科技有限公司 碱基簇检测方法及装置、基因测序仪及存储介质

Family Cites Families (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
FR2755149B1 (fr) * 1996-10-30 1999-01-15 Pasteur Institut Procede de diagnostic de maladies genetiques par peignage moleculaire et coffret de diagnostic
US7071324B2 (en) * 1998-10-13 2006-07-04 Brown University Research Foundation Systems and methods for sequencing by hybridization
KR20040036900A (ko) * 2001-07-15 2004-05-03 켁크 그래주에이트 인스티튜트 닉킹제를 사용한 핵산 단편의 증폭
US7276338B2 (en) * 2003-11-17 2007-10-02 Jacobson Joseph M Nucleotide sequencing via repetitive single molecule hybridization
WO2008134867A1 (fr) * 2007-05-04 2008-11-13 Genizon Biosciences Inc. Procédés, trousses et systèmes de séquençage d'acide nucléique par hybridation
WO2017087823A1 (fr) * 2015-11-18 2017-05-26 Mir Kalim U Séquençage super-résolution
EP3717645A4 (fr) * 2017-11-29 2021-09-01 XGenomes Corp. Séquençage d'acides nucléiques par émergence

Also Published As

Publication number Publication date
CN114207149A (zh) 2022-03-18
AU2020282704A1 (en) 2022-01-27
EP3976828A4 (fr) 2023-06-28
JP2022534920A (ja) 2022-08-04
WO2020243187A1 (fr) 2020-12-03
EP3976828A1 (fr) 2022-04-06
KR20220034054A (ko) 2022-03-17

Similar Documents

Publication Publication Date Title
US11427867B2 (en) Sequencing by emergence
US20240117413A1 (en) Sequencing by emergence
JP7532458B2 (ja) 化学組成物とそれを利用する方法
EP3411494B1 (fr) Séquençage super-résolution
WO2020243187A1 (fr) Séquençage par émergence
JP7457457B2 (ja) サンプル中の標的核酸を検出する方法
US20220359040A1 (en) Systems and methods for determining sequence
ES2984193T3 (es) Concatemerización de moldes en la superficie
US12509723B2 (en) Methods of sequencing with linked fragments
US20200082913A1 (en) Systems and methods for determining sequence
US10851411B2 (en) Molecular identification with subnanometer localization accuracy
US20220073980A1 (en) Sequencing by coalescence
HK40000881A (en) Super-resolution sequencing
HK40000881B (en) Super-resolution sequencing

Legal Events

Date Code Title Description
EEER Examination request

Effective date: 20240527

MFA Maintenance fee for application paid

Free format text: FEE DESCRIPTION TEXT: MF (APPLICATION, 5TH ANNIV.) - STANDARD

Year of fee payment: 5

U00 Fee paid

Free format text: ST27 STATUS EVENT CODE: A-2-2-U10-U00-U101 (AS PROVIDED BY THE NATIONAL OFFICE); EVENT TEXT: MAINTENANCE REQUEST RECEIVED

Effective date: 20251126

U11 Full renewal or maintenance fee paid

Free format text: ST27 STATUS EVENT CODE: A-2-2-U10-U11-U102 (AS PROVIDED BY THE NATIONAL OFFICE); EVENT TEXT: MAINTENANCE FEE PAYMENT PAID IN FULL

Effective date: 20251126

W00 Other event occurred

Free format text: ST27 STATUS EVENT CODE: A-2-2-W10-W00-W100 (AS PROVIDED BY THE NATIONAL OFFICE); EVENT TEXT: LETTER SENT

Effective date: 20251201