CA3146212C - Analyse de methylation du genome entier sans bisulfite - Google Patents

Analyse de methylation du genome entier sans bisulfite

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Publication number
CA3146212C
CA3146212C CA3146212A CA3146212A CA3146212C CA 3146212 C CA3146212 C CA 3146212C CA 3146212 A CA3146212 A CA 3146212A CA 3146212 A CA3146212 A CA 3146212A CA 3146212 C CA3146212 C CA 3146212C
Authority
CA
Canada
Prior art keywords
dna
target dna
5hmc
taps
5cac
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Active
Application number
CA3146212A
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English (en)
Other versions
CA3146212A1 (fr
Inventor
Chunxiao Song
Jingfei CHENG
Paulina SIEJKA-ZIELINSKA
Yibin Liu
Original Assignee
Ludwig Institute for Cancer Research Ltd
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Filing date
Publication date
Application filed by Ludwig Institute for Cancer Research Ltd filed Critical Ludwig Institute for Cancer Research Ltd
Priority claimed from PCT/IB2020/056435 external-priority patent/WO2021005537A1/fr
Publication of CA3146212A1 publication Critical patent/CA3146212A1/fr
Application granted granted Critical
Publication of CA3146212C publication Critical patent/CA3146212C/fr
Active legal-status Critical Current
Anticipated expiration legal-status Critical

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Abstract

La présente invention concerne des procédés économiques et sans bisulfite d'identification dans l'ADN, y compris dans l'intégralité de l'ADN génomique, de l'emplacement d'une ou plusieurs bases parmi la 5-méthylcytosine, la 5-hydroxyméthylcytosine, la 5-carboxylcytosine et la 5-formylcytosine. Les procédés décrits ici sont basés sur la conversion de la cytosine modifiée (5mC, 5hmC, 5fC, 5caC) en dihydrouracile (DHU), par exemple par traitement à la borane pyridine assisté par TET, suivi d'un clivage par endonucléase de la DHU et de l'identification du site de clivage, qui correspond à l'emplacement de la cytosine modifiée.
CA3146212A 2019-07-08 2020-07-08 Analyse de methylation du genome entier sans bisulfite Active CA3146212C (fr)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US201962871444P 2019-07-08 2019-07-08
US62/871,444 2019-07-08
PCT/IB2020/056435 WO2021005537A1 (fr) 2019-07-08 2020-07-08 Analyse de méthylation du génome entier sans bisulfite

Publications (2)

Publication Number Publication Date
CA3146212A1 CA3146212A1 (fr) 2021-01-14
CA3146212C true CA3146212C (fr) 2026-02-10

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