CA3147797C - Procede de coiffage d'arn enzymatique - Google Patents

Procede de coiffage d'arn enzymatique

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Publication number
CA3147797C
CA3147797C CA3147797A CA3147797A CA3147797C CA 3147797 C CA3147797 C CA 3147797C CA 3147797 A CA3147797 A CA 3147797A CA 3147797 A CA3147797 A CA 3147797A CA 3147797 C CA3147797 C CA 3147797C
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CA
Canada
Prior art keywords
rna
seq
capping
cap
enzyme
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Active
Application number
CA3147797A
Other languages
English (en)
Other versions
CA3147797A1 (fr
Inventor
G. Brett Robb
Siu-Hong Chan
Bijoyita Roy
Original Assignee
New England Biolabs Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by New England Biolabs Inc filed Critical New England Biolabs Inc
Priority claimed from PCT/US2020/047521 external-priority patent/WO2021041260A1/fr
Publication of CA3147797A1 publication Critical patent/CA3147797A1/fr
Application granted granted Critical
Publication of CA3147797C publication Critical patent/CA3147797C/fr
Active legal-status Critical Current
Anticipated expiration legal-status Critical

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Abstract

L'invention concerne un procédé de coiffage efficace D'ARN <i>in vitro</i>. Dans certains modes de réalisation, la réaction de coiffage peut être effectuée à haute température à l'aide d'une enzyme de coiffage de la vaccine ou d'un de ses variants. Dans d'autres modes de réalisation, les réactions de coiffage peuvent comprendre une enzyme de coiffage à partir d'un grand virus de l'amoeba, <i>par exemple</i>, le Faustovirus, le mimivirus ou,moumouvirus, ou un variant de ses variants. L'invention concerne aussi des compositions et des kits pour mettre en œuvre les procédés de l'invention.
CA3147797A 2019-08-23 2020-08-21 Procede de coiffage d'arn enzymatique Active CA3147797C (fr)

Applications Claiming Priority (5)

Application Number Priority Date Filing Date Title
US201962890821P 2019-08-23 2019-08-23
US62/890,821 2019-08-23
PCT/US2020/047521 WO2021041260A1 (fr) 2019-08-23 2020-08-21 Procédé de coiffage d'arn enzymatique
PCT/US2020/047533 WO2021041267A1 (fr) 2019-08-23 2020-08-21 Procédé de coiffage d'arn enzymatique
USPCT/US2020/047521 2020-08-21

Publications (2)

Publication Number Publication Date
CA3147797A1 CA3147797A1 (fr) 2021-03-04
CA3147797C true CA3147797C (fr) 2026-03-10

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