CA3179701A1 - Initiateurs reutilisables pour la synthese d'acides nucleiques - Google Patents

Initiateurs reutilisables pour la synthese d'acides nucleiques

Info

Publication number
CA3179701A1
CA3179701A1 CA3179701A CA3179701A CA3179701A1 CA 3179701 A1 CA3179701 A1 CA 3179701A1 CA 3179701 A CA3179701 A CA 3179701A CA 3179701 A CA3179701 A CA 3179701A CA 3179701 A1 CA3179701 A1 CA 3179701A1
Authority
CA
Canada
Prior art keywords
modified
nucleotide
group
exo
deoxyguanosine
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
Application number
CA3179701A
Other languages
English (en)
Inventor
Sanjay Agarwalla
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Molecular Assemblies Inc
Original Assignee
Molecular Assemblies Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from US16/841,214 external-priority patent/US11384377B2/en
Application filed by Molecular Assemblies Inc filed Critical Molecular Assemblies Inc
Publication of CA3179701A1 publication Critical patent/CA3179701A1/fr
Pending legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y207/00—Transferases transferring phosphorus-containing groups (2.7)
    • C12Y207/07—Nucleotidyltransferases (2.7.7)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00—Preparation of compounds containing saccharide radicals
    • C12P19/26—Preparation of nitrogen-containing carbohydrates
    • C12P19/28—N-glycosides
    • C12P19/30—Nucleotides
    • C12P19/34—Polynucleotides, e.g. nucleic acids, oligoribonucleotides
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
    • Y02P20/00—Technologies relating to chemical industry
    • Y02P20/50—Improvements relating to the production of bulk chemicals
    • Y02P20/55—Design of synthesis routes, e.g. reducing the use of auxiliary or protecting groups

Landscapes

  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Genetics & Genomics (AREA)
  • General Health & Medical Sciences (AREA)
  • General Engineering & Computer Science (AREA)
  • Biochemistry (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Microbiology (AREA)
  • Saccharide Compounds (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)

Abstract

L'invention concerne des procédés améliorés de synthèse de polynucléotides, tels que l'ADN et l'ARN, à l'aide d'initiateurs renouvelables couplés à un support solide. Au moyen des procédés de l'invention, des séquences spécifiques de polynucléotides peuvent être synthétisées de novo, base par base, dans un environnement aqueux, sans l'utilisation d'un modèle d'acide nucléique.
CA3179701A 2020-04-06 2021-04-06 Initiateurs reutilisables pour la synthese d'acides nucleiques Pending CA3179701A1 (fr)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US16/841,214 US11384377B2 (en) 2013-04-02 2020-04-06 Reusable initiators for synthesizing nucleic acids
US16/841,214 2020-04-06
PCT/US2021/025918 WO2021207158A1 (fr) 2020-04-06 2021-04-06 Initiateurs réutilisables pour la synthèse d'acides nucléiques

Publications (1)

Publication Number Publication Date
CA3179701A1 true CA3179701A1 (fr) 2021-10-14

Family

ID=78023444

Family Applications (1)

Application Number Title Priority Date Filing Date
CA3179701A Pending CA3179701A1 (fr) 2020-04-06 2021-04-06 Initiateurs reutilisables pour la synthese d'acides nucleiques

Country Status (6)

Country Link
EP (1) EP4133070A4 (fr)
JP (1) JP2023521704A (fr)
CN (1) CN116096882A (fr)
AU (1) AU2021252542A1 (fr)
CA (1) CA3179701A1 (fr)
WO (1) WO2021207158A1 (fr)

Family Cites Families (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5516664A (en) * 1992-12-23 1996-05-14 Hyman; Edward D. Enzymatic synthesis of repeat regions of oligonucleotides
US5872244A (en) * 1994-09-02 1999-02-16 Andrew C. Hiatt 3' protected nucleotides for enzyme catalyzed template-independent creation of phosphodiester bonds
SE9603171D0 (sv) * 1996-08-30 1996-08-30 Marek Kwiatkowski Solid phase synthesis
US10683536B2 (en) * 2013-04-02 2020-06-16 Molecular Assemblies, Inc. Reusable initiators for synthesizing nucleic acids
US8808989B1 (en) * 2013-04-02 2014-08-19 Molecular Assemblies, Inc. Methods and apparatus for synthesizing nucleic acids
US11331643B2 (en) * 2013-04-02 2022-05-17 Molecular Assemblies, Inc. Reusable initiators for synthesizing nucleic acids
JP6448097B2 (ja) * 2013-10-15 2019-01-09 モレキュラー アセンブリーズ， インコーポレイテッド 核酸を合成するための方法および装置
CA2958581C (fr) * 2014-08-18 2023-04-04 Molecular Assemblies, Inc. Procedes et appareil de synthese d'acides nucleiques
EP3826762A1 (fr) * 2018-07-23 2021-06-02 DNA Script Synthèse enzymatique massivement parallèle de brins d'acides nucléiques

Also Published As

Publication number Publication date
EP4133070A4 (fr) 2025-10-22
EP4133070A1 (fr) 2023-02-15
WO2021207158A1 (fr) 2021-10-14
JP2023521704A (ja) 2023-05-25
CN116096882A (zh) 2023-05-09
AU2021252542A1 (en) 2022-11-10

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US9695470B2 (en) Methods and apparatus for synthesizing nucleic acids
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JP7687954B2 (ja) 核酸を合成するための再使用可能な開始剤

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