CA3209578A1 - Emulsification avec des hydrogels magnetiques - Google Patents

Emulsification avec des hydrogels magnetiques Download PDF

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Publication number
CA3209578A1
CA3209578A1 CA3209578A CA3209578A CA3209578A1 CA 3209578 A1 CA3209578 A1 CA 3209578A1 CA 3209578 A CA3209578 A CA 3209578A CA 3209578 A CA3209578 A CA 3209578A CA 3209578 A1 CA3209578 A1 CA 3209578A1
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CA
Canada
Prior art keywords
analyte
hydrogel
sample
hydrogel beads
partitions
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Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
Application number
CA3209578A
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English (en)
Inventor
Yi XUE
Kristina FONTANEZ
Sepher Kiani
Robert MELTZER
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Fluent Biosciences Inc
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Fluent Biosciences Inc
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Publication date
Application filed by Fluent Biosciences Inc filed Critical Fluent Biosciences Inc
Publication of CA3209578A1 publication Critical patent/CA3209578A1/fr
Pending legal-status Critical Current

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1003—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor
    • C12N15/1006—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by means of a solid support carrier, e.g. particles, polymers
    • C12N15/1013—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by means of a solid support carrier, e.g. particles, polymers by using magnetic beads
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034—Isolating an individual clone by screening libraries
    • C12N15/1075—Isolating an individual clone by screening libraries by coupling phenotype to genotype, not provided for in other groups of this subclass
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
    • G01N33/54313—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals the carrier being characterised by its particulate form
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
    • G01N33/54313—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals the carrier being characterised by its particulate form
    • G01N33/54326—Magnetic particles
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
    • G01N33/54353—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals with ligand attached to the carrier via a chemical coupling agent
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
    • G01N33/544—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals the carrier being organic
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
    • G01N33/544—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals the carrier being organic
    • G01N33/545—Synthetic resin
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6806—Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Immunology (AREA)
  • Chemical & Material Sciences (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • Urology & Nephrology (AREA)
  • Hematology (AREA)
  • Biotechnology (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • Genetics & Genomics (AREA)
  • General Health & Medical Sciences (AREA)
  • Physics & Mathematics (AREA)
  • Analytical Chemistry (AREA)
  • Cell Biology (AREA)
  • Food Science & Technology (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Medicinal Chemistry (AREA)
  • Organic Chemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Physical Or Chemical Processes And Apparatus (AREA)

Abstract

La présente divulgation concerne de manière générale des procédés et des systèmes de préparation d'échantillons qui ne nécessitent pas de dispositifs microfluidiques coûteux ou certaines étapes chronophages, telles que la centrifugation. Au lieu de cela, les systèmes et les procédés de l'invention utilisent des hydrogels qui modélisent la formation de cloisons à l'intérieur d'émulsions et distinguent l'analyte à l'intérieur des cloisons modélisées. Les hydrogels ont un échafaudage d'hydrogel incorporé avec des nanoparticules magnétiques. La présence des nanoparticules magnétiques confère la capacité d'interagir avec les cloisons modélisées et de les manipuler. En outre, les hydrogels comprennent des liants moléculaires qui se lient à l'analyte cible à l'intérieur des cloisons rendant l'analyte cible sensible à des champs magnétiques pour manipuler l'analyte pendant la préparation d'échantillon.
CA3209578A 2021-01-25 2022-01-25 Emulsification avec des hydrogels magnetiques Pending CA3209578A1 (fr)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US202163141064P 2021-01-25 2021-01-25
US63/141,064 2021-01-25
PCT/US2022/013720 WO2022159896A2 (fr) 2021-01-25 2022-01-25 Émulsification avec des hydrogels magnétiques

Publications (1)

Publication Number Publication Date
CA3209578A1 true CA3209578A1 (fr) 2022-07-28

Family

ID=82549920

Family Applications (1)

Application Number Title Priority Date Filing Date
CA3209578A Pending CA3209578A1 (fr) 2021-01-25 2022-01-25 Emulsification avec des hydrogels magnetiques

Country Status (3)

Country Link
EP (1) EP4281586A4 (fr)
CA (1) CA3209578A1 (fr)
WO (1) WO2022159896A2 (fr)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2025029903A1 (fr) * 2023-08-01 2025-02-06 Pierce Biotechnology, Inc. Billes magnétiques fonctionnalisées et procédés de préparation et d'utilisation

Families Citing this family (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP3838268B1 (fr) 2017-02-24 2023-05-10 The Regents of the University of California Structures de particules-gouttes et procédé pour les préparer et les utiliser
CN115698282A (zh) 2020-01-13 2023-02-03 福路伦特生物科学公司 单细胞测序
WO2022221391A1 (fr) 2021-04-14 2022-10-20 Partillion Bioscience Corporation Chambres de réaction à l'échelle nanométrique et leurs procédés d'utilisation
US12534721B2 (en) 2022-10-06 2026-01-27 Illumina, Inc. Quantitative detection and analysis of moleculesip
CN120615168A (zh) * 2023-01-31 2025-09-09 基因泰克公司 磁性水凝胶颗粒及使用方法

Family Cites Families (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5512439A (en) * 1988-11-21 1996-04-30 Dynal As Oligonucleotide-linked magnetic particles and uses thereof
US5804684A (en) * 1995-08-24 1998-09-08 The Theobald Smith Research Institute, Inc. Method for isolating nucleic acids
US7914994B2 (en) * 1998-12-24 2011-03-29 Cepheid Method for separating an analyte from a sample
AU6380001A (en) * 2000-03-24 2001-10-03 Qiagen Gmbh Porous ferro- or ferrimagnetic glass particles for isolating molecules
US7527929B2 (en) * 2004-07-30 2009-05-05 Agencourt Bioscience Corporation Methods of isolating nucleic acids using multifunctional group-coated solid phase carriers
WO2019139650A2 (fr) * 2017-09-29 2019-07-18 The Regents Of The University Of California Procédé de génération d'émulsions monodispersées
WO2019076938A1 (fr) 2017-10-16 2019-04-25 Scherr Steven Systèmes et procédés d'encapsulation de particules dans des microgouttelettes
AU2019321593B2 (en) 2018-08-17 2025-01-23 The Regents Of The University Of California Particle-containing droplet systems with monodisperse fluid volumes
US20210340596A1 (en) * 2018-09-28 2021-11-04 Fluent Biosciences Inc. Target capture and barcoding in monodisperse droplets

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2025029903A1 (fr) * 2023-08-01 2025-02-06 Pierce Biotechnology, Inc. Billes magnétiques fonctionnalisées et procédés de préparation et d'utilisation

Also Published As

Publication number Publication date
EP4281586A2 (fr) 2023-11-29
WO2022159896A2 (fr) 2022-07-28
WO2022159896A3 (fr) 2022-09-01
EP4281586A4 (fr) 2024-12-25

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