CA3214973A1 - Enzymes variantes de guanylate kinase modifiees - Google Patents

Enzymes variantes de guanylate kinase modifiees Download PDF

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Publication number
CA3214973A1
CA3214973A1 CA3214973A CA3214973A CA3214973A1 CA 3214973 A1 CA3214973 A1 CA 3214973A1 CA 3214973 A CA3214973 A CA 3214973A CA 3214973 A CA3214973 A CA 3214973A CA 3214973 A1 CA3214973 A1 CA 3214973A1
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CA
Canada
Prior art keywords
engineered
guanylate kinase
sequence
seq
polypeptide
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
Application number
CA3214973A
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English (en)
Inventor
Da DUAN
Auric Anthony Sowell-Kantz
Aksiniya Lyubenova Petkova
Oscar Alvizo
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Codexis Inc
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Codexis Inc
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Publication date
Application filed by Codexis Inc filed Critical Codexis Inc
Publication of CA3214973A1 publication Critical patent/CA3214973A1/fr
Pending legal-status Critical Current

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/52—Genes encoding for enzymes or proenzymes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/12—Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)
    • C12N9/1229—Phosphotransferases with a phosphate group as acceptor (2.7.4)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/12—Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00—Preparation of compounds containing saccharide radicals
    • C12P19/26—Preparation of nitrogen-containing carbohydrates
    • C12P19/28—N-glycosides
    • C12P19/30—Nucleotides
    • C12P19/32—Nucleotides having a condensed ring system containing a six-membered ring having two N-atoms in the same ring, e.g. purine nucleotides, nicotineamide-adenine dinucleotide
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y207/00—Transferases transferring phosphorus-containing groups (2.7)
    • C12Y207/04—Phosphotransferases with a phosphate group as acceptor (2.7.4)
    • C12Y207/04008—Guanylate kinase (2.7.4.8)

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • Molecular Biology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Medicinal Chemistry (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)

Abstract

La présente invention concerne des enzymes guanylate kinase (GK) modifiées, des polypeptides ayant une activité GK, et des polynucléotides codant pour ces enzymes, ainsi que des vecteurs et des cellules hôtes comprenant ces polynucléotides et polypeptides. L'invention concerne également des méthodes de production d'enzymes GK. La présente invention concerne en outre des compositions comprenant les enzymes GK et des méthodes d'utilisation des enzymes GK modifiées. La présente invention trouve une utilisation particulière dans la production de composés pharmaceutiques.
CA3214973A 2021-04-02 2022-04-01 Enzymes variantes de guanylate kinase modifiees Pending CA3214973A1 (fr)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US202163170004P 2021-04-02 2021-04-02
US63/170,004 2021-04-02
PCT/US2022/023030 WO2022212828A1 (fr) 2021-04-02 2022-04-01 Enzymes variantes de guanylate kinase modifiées

Publications (1)

Publication Number Publication Date
CA3214973A1 true CA3214973A1 (fr) 2022-10-06

Family

ID=81388761

Family Applications (1)

Application Number Title Priority Date Filing Date
CA3214973A Pending CA3214973A1 (fr) 2021-04-02 2022-04-01 Enzymes variantes de guanylate kinase modifiees

Country Status (6)

Country Link
US (1) US12473557B2 (fr)
EP (1) EP4314262A1 (fr)
CN (1) CN117120599A (fr)
CA (1) CA3214973A1 (fr)
IL (1) IL305922A (fr)
WO (1) WO2022212828A1 (fr)

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Also Published As

Publication number Publication date
US20220325284A1 (en) 2022-10-13
WO2022212828A1 (fr) 2022-10-06
IL305922A (en) 2023-11-01
CN117120599A (zh) 2023-11-24
EP4314262A1 (fr) 2024-02-07
US12473557B2 (en) 2025-11-18

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