CA3234821A1 - Methodes de culture de cellules immunitaires - Google Patents

Methodes de culture de cellules immunitaires Download PDF

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Publication number
CA3234821A1
CA3234821A1 CA3234821A CA3234821A CA3234821A1 CA 3234821 A1 CA3234821 A1 CA 3234821A1 CA 3234821 A CA3234821 A CA 3234821A CA 3234821 A CA3234821 A CA 3234821A CA 3234821 A1 CA3234821 A1 CA 3234821A1
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Prior art keywords
tils
aspects
culture
concentration
less
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Pending
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CA3234821A
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English (en)
Inventor
Suman Kumar VODNALA
Yogin PATEL
Nicholas P. Restifo
Benjamin Harris
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Lyell Immunopharma Inc
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Lyell Immunopharma Inc
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Publication of CA3234821A1 publication Critical patent/CA3234821A1/fr
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    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0634—Cells from the blood or the immune system
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K40/00—Cellular immunotherapy
    • A61K40/10—Cellular immunotherapy characterised by the cell type used
    • A61K40/11—T-cells, e.g. tumour infiltrating lymphocytes [TIL] or regulatory T [Treg] cells; Lymphokine-activated killer [LAK] cells
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K40/00—Cellular immunotherapy
    • A61K40/40—Cellular immunotherapy characterised by antigens that are targeted or presented by cells of the immune system
    • A61K40/41—Vertebrate antigens
    • A61K40/42—Cancer antigens
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P35/00—Antineoplastic agents
    • A61P35/02—Antineoplastic agents specific for leukemia
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    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0634—Cells from the blood or the immune system
    • C12N5/0636—T lymphocytes
    • C—CHEMISTRY; METALLURGY
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    • C12N2500/00—Specific components of cell culture medium
    • C12N2500/05—Inorganic components
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    • C12N2500/00—Specific components of cell culture medium
    • C12N2500/05—Inorganic components
    • C12N2500/10—Metals; Metal chelators
    • C12N2500/12—Light metals, i.e. alkali, alkaline earth, Be, Al, Mg
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    • C12N2500/00—Specific components of cell culture medium
    • C12N2500/05—Inorganic components
    • C12N2500/10—Metals; Metal chelators
    • C12N2500/12—Light metals, i.e. alkali, alkaline earth, Be, Al, Mg
    • C12N2500/14—Calcium; Ca chelators; Calcitonin
    • C—CHEMISTRY; METALLURGY
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    • C12N2500/00—Specific components of cell culture medium
    • C12N2500/30—Organic components
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12N2500/00—Specific components of cell culture medium
    • C12N2500/30—Organic components
    • C12N2500/34—Sugars
    • C—CHEMISTRY; METALLURGY
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    • C12N2500/00—Specific components of cell culture medium
    • C12N2500/30—Organic components
    • C12N2500/38—Vitamins
    • C—CHEMISTRY; METALLURGY
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    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/20—Cytokines; Chemokines
    • C12N2501/23—Interleukins [IL]
    • C12N2501/2302—Interleukin-2 (IL-2)
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    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/20—Cytokines; Chemokines
    • C12N2501/23—Interleukins [IL]
    • C12N2501/2307—Interleukin-7 (IL-7)
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    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/20—Cytokines; Chemokines
    • C12N2501/23—Interleukins [IL]
    • C12N2501/2315—Interleukin-15 (IL-15)
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    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/20—Cytokines; Chemokines
    • C12N2501/23—Interleukins [IL]
    • C12N2501/2321—Interleukin-21 (IL-21)
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    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/50—Cell markers; Cell surface determinants
    • C12N2501/51—B7 molecules, e.g. CD80, CD86, CD28 (ligand), CD152 (ligand)
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    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/50—Cell markers; Cell surface determinants
    • C12N2501/515—CD3, T-cell receptor complex
    • C—CHEMISTRY; METALLURGY
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    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/70—Enzymes
    • C12N2501/72—Transferases [EC 2.]
    • C12N2501/727—Kinases (EC 2.7.)
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    • C12N2502/30—Coculture with; Conditioned medium produced by tumour cells
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    • C12N2527/00—Culture process characterised by the use of mechanical forces, e.g. strain, vibration

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  • Chemical Kinetics & Catalysis (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • Oncology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Medicines Containing Material From Animals Or Micro-Organisms (AREA)
  • Developmental Biology & Embryology (AREA)
  • Virology (AREA)
  • Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
  • Medicines Containing Antibodies Or Antigens For Use As Internal Diagnostic Agents (AREA)

Abstract

La présente divulgation concerne des méthodes de culture de TIL dans un milieu comprenant des ions potassium à une concentration d'au moins environ 30 mM à au moins environ 100 mM. Selon certains aspects, les méthodes divulguées ici améliorent l'expansion des TIL CD8+, par rapport aux TIL CD4+. Selon certains aspects, les méthodes divulguées ici augmentent le nombre de cellules moins différenciées, par exemple de TIL moins différenciés, dans la population de cellules. Selon certains aspects, les méthodes divulguées ici assurent un enrichissement en TIL réagissant aux tumeurs, par exemple spécifiques à une tumeur, de façon à préserver la diversité clonale. Selon certains aspects, les cellules, par exemple les TIL, sont administrées à un sujet en ayant besoin.
CA3234821A 2021-10-28 2022-10-27 Methodes de culture de cellules immunitaires Pending CA3234821A1 (fr)

Applications Claiming Priority (7)

Application Number Priority Date Filing Date Title
US202163273138P 2021-10-28 2021-10-28
US63/273,138 2021-10-28
US202263365326P 2022-05-25 2022-05-25
US63/365,326 2022-05-25
US202263379632P 2022-10-14 2022-10-14
US63/379,632 2022-10-14
PCT/US2022/078827 WO2023077034A1 (fr) 2021-10-28 2022-10-27 Méthodes de culture de cellules immunitaires

Publications (1)

Publication Number Publication Date
CA3234821A1 true CA3234821A1 (fr) 2023-05-04

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ID=84604187

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CA3234821A Pending CA3234821A1 (fr) 2021-10-28 2022-10-27 Methodes de culture de cellules immunitaires

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EP (1) EP4423249A1 (fr)
JP (1) JP2024540106A (fr)
KR (1) KR20240099331A (fr)
AU (1) AU2022377637A1 (fr)
CA (1) CA3234821A1 (fr)
IL (1) IL312027A (fr)
WO (1) WO2023077034A1 (fr)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2025006811A1 (fr) * 2023-06-27 2025-01-02 Lyell Immunopharma, Inc. Méthodes de culture de cellules immunitaires

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Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2025006811A1 (fr) * 2023-06-27 2025-01-02 Lyell Immunopharma, Inc. Méthodes de culture de cellules immunitaires

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EP4423249A1 (fr) 2024-09-04
WO2023077034A1 (fr) 2023-05-04
JP2024540106A (ja) 2024-10-31
KR20240099331A (ko) 2024-06-28
AU2022377637A1 (en) 2024-05-02
IL312027A (en) 2024-06-01

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