CA3241703A1 - Enzymes ayant des domaines ruvc - Google Patents

Enzymes ayant des domaines ruvc

Info

Publication number
CA3241703A1
CA3241703A1 CA3241703A CA3241703A CA3241703A1 CA 3241703 A1 CA3241703 A1 CA 3241703A1 CA 3241703 A CA3241703 A CA 3241703A CA 3241703 A CA3241703 A CA 3241703A CA 3241703 A1 CA3241703 A1 CA 3241703A1
Authority
CA
Canada
Prior art keywords
enzymes
ruvc domains
ruvc
domains
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Granted
Application number
CA3241703A
Other languages
English (en)
Other versions
CA3241703C (fr
Inventor
Brian Thomas
Christopher Brown
Rose KANTOR
Audra DEVOTO
Cristina Butterfield
Lisa ALEXANDER
Daniela S. A. GOLTSMAN
Jason Liu
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Metagenomi Therapeutics Inc
Original Assignee
Metagenomi Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Metagenomi Inc filed Critical Metagenomi Inc
Priority to CA3241703A priority Critical patent/CA3241703C/fr
Publication of CA3241703A1 publication Critical patent/CA3241703A1/fr
Application granted granted Critical
Publication of CA3241703C publication Critical patent/CA3241703C/fr
Active legal-status Critical Current
Anticipated expiration legal-status Critical

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    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14Hydrolases (3)
    • C12N9/16Hydrolases (3) acting on ester bonds (3.1)
    • C12N9/22Ribonucleases [RNase]; Deoxyribonucleases [DNase]
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/10Processes for the isolation, preparation or purification of DNA or RNA
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/10Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/102Mutagenizing nucleic acids
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/11DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/11DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/113Non-coding nucleic acids modulating the expression of genes, e.g. antisense oligonucleotides; Antisense DNA or RNA; Triplex- forming oligonucleotides; Catalytic nucleic acids, e.g. ribozymes; Nucleic acids used in co-suppression or gene silencing
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/87Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
    • C12N15/90Stable introduction of foreign DNA into chromosome
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/87Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
    • C12N15/90Stable introduction of foreign DNA into chromosome
    • C12N15/902Stable introduction of foreign DNA into chromosome using homologous recombination
    • C12N15/907Stable introduction of foreign DNA into chromosome using homologous recombination in mammalian cells
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12YENZYMES
    • C12Y301/00Hydrolases acting on ester bonds (3.1)
    • C12Y301/21Endodeoxyribonucleases producing 5'-phosphomonoesters (3.1.21)
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12N2310/00Structure or type of the nucleic acid
    • C12N2310/10Type of nucleic acid
    • C12N2310/20Type of nucleic acid involving clustered regularly interspaced short palindromic repeats [CRISPR]
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2310/00Structure or type of the nucleic acid
    • C12N2310/50Physical structure
    • C12N2310/53Physical structure partially self-complementary or closed
    • C12N2310/531Stem-loop; Hairpin

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Physics & Mathematics (AREA)
  • Medicinal Chemistry (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Mycology (AREA)
  • Cell Biology (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Immobilizing And Processing Of Enzymes And Microorganisms (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Peptides Or Proteins (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
CA3241703A 2019-02-14 2020-02-14 Enzymes ayant des domaines ruvc Active CA3241703C (fr)

Priority Applications (1)

Application Number Priority Date Filing Date Title
CA3241703A CA3241703C (fr) 2019-02-14 2020-02-14 Enzymes ayant des domaines ruvc

Applications Claiming Priority (10)

Application Number Priority Date Filing Date Title
US201962805899P 2019-02-14 2019-02-14
US201962805868P 2019-02-14 2019-02-14
US201962805878P 2019-02-14 2019-02-14
US62/805,868 2019-02-14
US62/805,878 2019-02-14
US62/805,899 2019-02-14
US201962874414P 2019-07-15 2019-07-15
US62/874,414 2019-07-15
CA3241703A CA3241703C (fr) 2019-02-14 2020-02-14 Enzymes ayant des domaines ruvc
CA3130135A CA3130135C (fr) 2019-02-14 2020-02-14 Enzymes ayant des domaines ruvc

Related Parent Applications (1)

Application Number Title Priority Date Filing Date
CA3130135A Division CA3130135C (fr) 2019-02-14 2020-02-14 Enzymes ayant des domaines ruvc

Publications (2)

Publication Number Publication Date
CA3241703A1 true CA3241703A1 (fr) 2020-08-20
CA3241703C CA3241703C (fr) 2026-02-24

Family

ID=72045639

Family Applications (2)

Application Number Title Priority Date Filing Date
CA3241703A Active CA3241703C (fr) 2019-02-14 2020-02-14 Enzymes ayant des domaines ruvc
CA3130135A Active CA3130135C (fr) 2019-02-14 2020-02-14 Enzymes ayant des domaines ruvc

Family Applications After (1)

Application Number Title Priority Date Filing Date
CA3130135A Active CA3130135C (fr) 2019-02-14 2020-02-14 Enzymes ayant des domaines ruvc

Country Status (9)

Country Link
US (1) US20240117330A1 (fr)
EP (1) EP3924482A4 (fr)
JP (3) JP2022520428A (fr)
KR (2) KR20240007322A (fr)
CN (2) CN116515797A (fr)
AU (3) AU2020223370B2 (fr)
CA (2) CA3241703C (fr)
MX (2) MX2021009886A (fr)
WO (1) WO2020168291A1 (fr)

Families Citing this family (26)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2021178933A2 (fr) 2020-03-06 2021-09-10 Metagenomi Ip Technologies, Llc Systèmes crispr de classe ii, de type v
AU2015330699B2 (en) 2014-10-10 2021-12-02 Editas Medicine, Inc. Compositions and methods for promoting homology directed repair
EP3823633A4 (fr) 2018-06-29 2023-05-03 Editas Medicine, Inc. Molécules de guidage synthétiques, compositions et procédés associés
WO2021226363A1 (fr) * 2020-05-08 2021-11-11 Metagenomi Ip Technologies, Llc Enzymes à domaines ruvc
CA3241703C (fr) * 2019-02-14 2026-02-24 Metagenomi Therapeutics, Inc. Enzymes ayant des domaines ruvc
US10913941B2 (en) 2019-02-14 2021-02-09 Metagenomi Ip Technologies, Llc Enzymes with RuvC domains
GB2594339B (en) * 2020-02-14 2023-02-08 Metagenomi Inc Enzymes with RUVC domains
WO2021202568A1 (fr) 2020-03-31 2021-10-07 Metagenomi Ip Technologies, Llc Systèmes crispr de classe ii, type ii
EP4165177A4 (fr) * 2020-05-08 2024-08-28 Metagenomi, Inc. Enzymes à domaines ruvc
JP2023540797A (ja) 2020-09-11 2023-09-26 メタゲノミ,インコーポレイテッド 塩基編集酵素
GB2700205B (en) * 2020-09-11 2026-04-15 Metagenomi Therapeutics Inc Base editing enzymes
WO2022087135A1 (fr) * 2020-10-21 2022-04-28 Emendobio Inc. Nouvelles nucléases crispr omni-59, 58, 65, 68, 71, 75, 78 et 84
WO2022098681A2 (fr) * 2020-11-03 2022-05-12 Caspr Biotech Corporation Nouvelles endonucléases guidées par un arn crispr-cas de classe 2
CA3205865A1 (fr) * 2021-01-22 2022-07-28 Brian C. Thomas Nouvelles nucleases ingenierisees et chimeriques
WO2022159742A1 (fr) * 2021-01-22 2022-07-28 Metagenomi, Inc Nouvelles nucléases modifiées et chimériques
JP2024515936A (ja) * 2021-03-19 2024-04-11 メタゲノミ,インク. Cas酵素を用いたマルチプレックス編集
KR20240004618A (ko) * 2021-04-30 2024-01-11 메타지노미, 인크. Ruvc 도메인을 갖는 효소
AU2022335499A1 (en) 2021-08-27 2024-02-22 Metagenomi Therapeutics, Inc. Enzymes with ruvc domains
WO2023081855A1 (fr) 2021-11-05 2023-05-11 Metagenomi, Inc. Enzymes d'édition de base
AU2022396533A1 (en) 2021-11-24 2024-05-02 Metagenomi Therapeutics, Inc. Endonuclease systems
WO2023122663A2 (fr) * 2021-12-23 2023-06-29 Mammoth Biosciences, Inc. Protéines effectrices et procédés d'utilisation
WO2023194359A1 (fr) * 2022-04-04 2023-10-12 Alia Therapeutics Srl Compositions et méthodes de traitement du syndrome d'usher de type 2a
KR20250037788A (ko) * 2022-06-15 2025-03-18 빔 테라퓨틱스, 인크. 보체 활성화를 감소시키기 위한 조성물 및 방법
CA3267752A1 (fr) * 2022-09-16 2024-03-21 Alia Therapeutics Srl Protéines cas de type ii enqp et leurs applications
AU2024230342A1 (en) * 2023-03-01 2025-10-16 Metagenomi Therapeutics, Inc. Enzymes with ruvc domains
WO2025106934A1 (fr) * 2023-11-16 2025-05-22 Metagenomi, Inc. Enzymes à domaines ruvc

Family Cites Families (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN113355357B (zh) * 2012-12-12 2024-12-03 布罗德研究所有限公司 对用于序列操纵的改进的系统、方法和酶组合物进行的工程化和优化
WO2016196655A1 (fr) * 2015-06-03 2016-12-08 The Regents Of The University Of California Variants de cas9 et procédés d'utilisation associés
WO2016205759A1 (fr) * 2015-06-18 2016-12-22 The Broad Institute Inc. Modification et optimisation de systèmes, de méthodes, d'enzymes et d'échafaudages guides d'orthologues de cas9 et variant pour la manipulation de séquences
EP3500967A1 (fr) * 2016-08-17 2019-06-26 The Broad Institute, Inc. Méthodes d'identification de systèmes crispr-cas de classe 2
WO2019165168A1 (fr) * 2018-02-23 2019-08-29 Pioneer Hi-Bred International, Inc. Nouveaux orthologues de cas9
CA3241703C (fr) * 2019-02-14 2026-02-24 Metagenomi Therapeutics, Inc. Enzymes ayant des domaines ruvc

Also Published As

Publication number Publication date
EP3924482A1 (fr) 2021-12-22
US20240117330A1 (en) 2024-04-11
CA3130135C (fr) 2024-11-19
KR102623312B1 (ko) 2024-01-09
CA3130135A1 (fr) 2020-08-20
MX2021009886A (es) 2021-10-13
CA3241703C (fr) 2026-02-24
MX2023006575A (es) 2023-06-16
KR20240007322A (ko) 2024-01-16
AU2020223370A1 (en) 2021-10-07
AU2025279720A1 (en) 2026-01-15
JP7502537B2 (ja) 2024-06-18
JP2022520428A (ja) 2022-03-30
JP2024133476A (ja) 2024-10-02
JP2023179468A (ja) 2023-12-19
CN116515797A (zh) 2023-08-01
AU2020223370B2 (en) 2023-04-20
EP3924482A4 (fr) 2023-04-05
CN113728098A (zh) 2021-11-30
WO2020168291A1 (fr) 2020-08-20
KR20210139254A (ko) 2021-11-22
AU2023206079A1 (en) 2023-08-10

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