CH633582A5 - Verfahren zur bestimmung des amylasegehalts von proben und mittel zur ausfuehrung des verfahrens. - Google Patents

Verfahren zur bestimmung des amylasegehalts von proben und mittel zur ausfuehrung des verfahrens. Download PDF

Info

Publication number: CH633582A5
Authority: CH; Switzerland
Prior art keywords: substrate; formula; solution; maltase; radical
Prior art date: 1976-07-13

Application number

CH864077A

Other languages

German (de)

English (en)

Inventor

Robert Carroll Menson

Venkatachalam Narayanswamy

Richard Charles Burns

Original Assignee

Du Pont

Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)

1976-07-13

Filing date

1977-07-12

Publication date

1982-12-15

1977-07-12 Application filed by Du Pont filed Critical Du Pont

1982-12-15 Publication of CH633582A5 publication Critical patent/CH633582A5/de

Links

239000004382 Amylase Substances 0.000 title claims description 33
238000000034 method Methods 0.000 title claims description 23
108010065511 Amylases Proteins 0.000 title claims description 18
102000013142 Amylases Human genes 0.000 title claims description 18
235000019418 amylase Nutrition 0.000 title claims description 18
239000000758 substrate Substances 0.000 claims description 58
108010028144 alpha-Glucosidases Proteins 0.000 claims description 21
102000016679 alpha-Glucosidases Human genes 0.000 claims description 19
150000004676 glycans Chemical class 0.000 claims description 15
229920001282 polysaccharide Polymers 0.000 claims description 15
239000005017 polysaccharide Substances 0.000 claims description 15
125000000636 p-nitrophenyl group Chemical group [H]C1=C([H])C(=C([H])C([H])=C1*)[N+]([O-])=O 0.000 claims description 14
-1 aromatic radical Chemical class 0.000 claims description 12
238000012360 testing method Methods 0.000 claims description 12
230000008033 biological extinction Effects 0.000 claims description 10
239000003153 chemical reaction reagent Substances 0.000 claims description 9
150000002338 glycosides Chemical group 0.000 claims description 7
125000004432 carbon atom Chemical group C* 0.000 claims description 6
FJCUPROCOFFUSR-GMMZZHHDSA-N maltopentaose Chemical compound O[C@@H]1[C@@H](O)[C@@H](O[C@H]([C@H](O)CO)[C@H](O)[C@@H](O)C=O)O[C@H](CO)[C@H]1O[C@@H]1[C@H](O)[C@@H](O)[C@H](O[C@@H]2[C@@H]([C@@H](O)[C@H](O[C@@H]3[C@@H]([C@@H](O)[C@H](O)[C@@H](CO)O3)O)[C@@H](CO)O2)O)[C@@H](CO)O1 FJCUPROCOFFUSR-GMMZZHHDSA-N 0.000 claims description 6
230000003595 spectral effect Effects 0.000 claims description 6
FTNIPWXXIGNQQF-UHFFFAOYSA-N UNPD130147 Natural products OC1C(O)C(O)C(CO)OC1OC1C(CO)OC(OC2C(OC(OC3C(OC(OC4C(OC(O)C(O)C4O)CO)C(O)C3O)CO)C(O)C2O)CO)C(O)C1O FTNIPWXXIGNQQF-UHFFFAOYSA-N 0.000 claims description 5
LUEWUZLMQUOBSB-UHFFFAOYSA-N UNPD55895 Natural products OC1C(O)C(O)C(CO)OC1OC1C(CO)OC(OC2C(OC(OC3C(OC(O)C(O)C3O)CO)C(O)C2O)CO)C(O)C1O LUEWUZLMQUOBSB-UHFFFAOYSA-N 0.000 claims description 5
OCIBBXPLUVYKCH-QXVNYKTNSA-N alpha-maltohexaose Chemical compound O[C@@H]1[C@@H](O)[C@H](O)[C@@H](CO)O[C@@H]1O[C@@H]1[C@@H](CO)O[C@H](O[C@@H]2[C@H](O[C@H](O[C@@H]3[C@H](O[C@H](O[C@@H]4[C@H](O[C@H](O[C@@H]5[C@H](O[C@H](O)[C@H](O)[C@H]5O)CO)[C@H](O)[C@H]4O)CO)[C@H](O)[C@H]3O)CO)[C@H](O)[C@H]2O)CO)[C@H](O)[C@H]1O OCIBBXPLUVYKCH-QXVNYKTNSA-N 0.000 claims description 5
DJMVHSOAUQHPSN-UHFFFAOYSA-N malto-hexaose Natural products OC1C(O)C(OC(C(O)CO)C(O)C(O)C=O)OC(CO)C1OC1C(O)C(O)C(OC2C(C(O)C(OC3C(C(O)C(OC4C(C(O)C(O)C(CO)O4)O)C(CO)O3)O)C(CO)O2)O)C(CO)O1 DJMVHSOAUQHPSN-UHFFFAOYSA-N 0.000 claims description 5
FJCUPROCOFFUSR-UHFFFAOYSA-N malto-pentaose Natural products OC1C(O)C(OC(C(O)CO)C(O)C(O)C=O)OC(CO)C1OC1C(O)C(O)C(OC2C(C(O)C(OC3C(C(O)C(O)C(CO)O3)O)C(CO)O2)O)C(CO)O1 FJCUPROCOFFUSR-UHFFFAOYSA-N 0.000 claims description 5
UYQJCPNSAVWAFU-UHFFFAOYSA-N malto-tetraose Natural products OC1C(O)C(OC(C(O)CO)C(O)C(O)C=O)OC(CO)C1OC1C(O)C(O)C(OC2C(C(O)C(O)C(CO)O2)O)C(CO)O1 UYQJCPNSAVWAFU-UHFFFAOYSA-N 0.000 claims description 5
LUEWUZLMQUOBSB-OUBHKODOSA-N maltotetraose Chemical compound O[C@H]1[C@H](O)[C@@H](O)[C@H](CO)O[C@H]1O[C@@H]1[C@H](CO)O[C@@H](O[C@@H]2[C@@H](O[C@@H](O[C@@H]3[C@@H](O[C@@H](O)[C@H](O)[C@H]3O)CO)[C@H](O)[C@H]2O)CO)[C@H](O)[C@H]1O LUEWUZLMQUOBSB-OUBHKODOSA-N 0.000 claims description 5
150000003254 radicals Chemical class 0.000 claims description 5
229930182470 glycoside Natural products 0.000 claims description 4
TWCMVXMQHSVIOJ-UHFFFAOYSA-N Aglycone of yadanzioside D Natural products COC(=O)C12OCC34C(CC5C(=CC(O)C(O)C5(C)C3C(O)C1O)C)OC(=O)C(OC(=O)C)C24 TWCMVXMQHSVIOJ-UHFFFAOYSA-N 0.000 claims description 3
PLMKQQMDOMTZGG-UHFFFAOYSA-N Astrantiagenin E-methylester Natural products CC12CCC(O)C(C)(CO)C1CCC1(C)C2CC=C2C3CC(C)(C)CCC3(C(=O)OC)CCC21C PLMKQQMDOMTZGG-UHFFFAOYSA-N 0.000 claims description 3
125000000217 alkyl group Chemical group 0.000 claims description 3
229910052736 halogen Inorganic materials 0.000 claims description 3
150000002367 halogens Chemical class 0.000 claims description 3
PFOARMALXZGCHY-UHFFFAOYSA-N homoegonol Natural products C1=C(OC)C(OC)=CC=C1C1=CC2=CC(CCCO)=CC(OC)=C2O1 PFOARMALXZGCHY-UHFFFAOYSA-N 0.000 claims description 3
ZHCAAFJSYLFLPX-UHFFFAOYSA-N nitrocyclohexatriene Chemical compound [O-][N+](=O)C1=CC=C=C[CH]1 ZHCAAFJSYLFLPX-UHFFFAOYSA-N 0.000 claims description 3
CBOIHMRHGLHBPB-UHFFFAOYSA-N hydroxymethyl Chemical compound O[CH2] CBOIHMRHGLHBPB-UHFFFAOYSA-N 0.000 claims 2
238000006243 chemical reaction Methods 0.000 description 23
239000000523 sample Substances 0.000 description 19
230000035945 sensitivity Effects 0.000 description 16
102000004190 Enzymes Human genes 0.000 description 11
108090000790 Enzymes Proteins 0.000 description 11
229940088598 enzyme Drugs 0.000 description 11
WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 description 8
150000001875 compounds Chemical class 0.000 description 8
239000008103 glucose Substances 0.000 description 8
BTJIUGUIPKRLHP-UHFFFAOYSA-N 4-nitrophenol Chemical compound OC1=CC=C([N+]([O-])=O)C=C1 BTJIUGUIPKRLHP-UHFFFAOYSA-N 0.000 description 6
OKKJLVBELUTLKV-UHFFFAOYSA-N Methanol Chemical compound OC OKKJLVBELUTLKV-UHFFFAOYSA-N 0.000 description 6
239000003085 diluting agent Substances 0.000 description 6
125000002791 glucosyl group Chemical group C1([C@H](O)[C@@H](O)[C@H](O)[C@H](O1)CO)* 0.000 description 6
229920001542 oligosaccharide Polymers 0.000 description 6
150000002482 oligosaccharides Chemical class 0.000 description 6
230000007062 hydrolysis Effects 0.000 description 5
238000006460 hydrolysis reaction Methods 0.000 description 5
HEMHJVSKTPXQMS-UHFFFAOYSA-M sodium hydroxide Inorganic materials [OH-].[Na+] HEMHJVSKTPXQMS-UHFFFAOYSA-M 0.000 description 5
238000001514 detection method Methods 0.000 description 4
238000005457 optimization Methods 0.000 description 4
150000002989 phenols Chemical class 0.000 description 4
239000012064 sodium phosphate buffer Substances 0.000 description 4
238000011156 evaluation Methods 0.000 description 3
238000002474 experimental method Methods 0.000 description 3
229920000642 polymer Polymers 0.000 description 3
210000002966 serum Anatomy 0.000 description 3
239000000126 substance Substances 0.000 description 3
BTJIUGUIPKRLHP-UHFFFAOYSA-M 4-nitrophenolate Chemical compound [O-]C1=CC=C([N+]([O-])=O)C=C1 BTJIUGUIPKRLHP-UHFFFAOYSA-M 0.000 description 2
102100024295 Maltase-glucoamylase Human genes 0.000 description 2
WQDUMFSSJAZKTM-UHFFFAOYSA-N Sodium methoxide Chemical compound [Na+].[O-]C WQDUMFSSJAZKTM-UHFFFAOYSA-N 0.000 description 2
238000002835 absorbance Methods 0.000 description 2
238000004458 analytical method Methods 0.000 description 2
150000001450 anions Chemical class 0.000 description 2
238000004140 cleaning Methods 0.000 description 2
230000007423 decrease Effects 0.000 description 2
230000001419 dependent effect Effects 0.000 description 2
125000003147 glycosyl group Chemical group 0.000 description 2
210000003296 saliva Anatomy 0.000 description 2
SQGYOTSLMSWVJD-UHFFFAOYSA-N silver(1+) nitrate Chemical compound [Ag+].[O-]N(=O)=O SQGYOTSLMSWVJD-UHFFFAOYSA-N 0.000 description 2
210000002700 urine Anatomy 0.000 description 2
OWEGMIWEEQEYGQ-UHFFFAOYSA-N 100676-05-9 Natural products OC1C(O)C(O)C(CO)OC1OCC1C(O)C(O)C(O)C(OC2C(OC(O)C(O)C2O)CO)O1 OWEGMIWEEQEYGQ-UHFFFAOYSA-N 0.000 description 1
QTBSBXVTEAMEQO-UHFFFAOYSA-M Acetate Chemical compound CC([O-])=O QTBSBXVTEAMEQO-UHFFFAOYSA-M 0.000 description 1
241000282412 Homo Species 0.000 description 1
GUBGYTABKSRVRQ-PICCSMPSSA-N Maltose Natural products O[C@@H]1[C@@H](O)[C@H](O)[C@@H](CO)O[C@@H]1O[C@@H]1[C@@H](CO)OC(O)[C@H](O)[C@H]1O GUBGYTABKSRVRQ-PICCSMPSSA-N 0.000 description 1
206010033645 Pancreatitis Diseases 0.000 description 1
206010033647 Pancreatitis acute Diseases 0.000 description 1
ISWSIDIOOBJBQZ-UHFFFAOYSA-N Phenol Chemical compound OC1=CC=CC=C1 ISWSIDIOOBJBQZ-UHFFFAOYSA-N 0.000 description 1
229920005654 Sephadex Polymers 0.000 description 1
239000012507 Sephadex™ Substances 0.000 description 1
229920002472 Starch Polymers 0.000 description 1
150000001242 acetic acid derivatives Chemical class 0.000 description 1
239000013543 active substance Substances 0.000 description 1
201000003229 acute pancreatitis Diseases 0.000 description 1
102000004139 alpha-Amylases Human genes 0.000 description 1
108090000637 alpha-Amylases Proteins 0.000 description 1
229940024171 alpha-amylase Drugs 0.000 description 1
238000013459 approach Methods 0.000 description 1
125000003118 aryl group Chemical group 0.000 description 1
238000003556 assay Methods 0.000 description 1
210000001124 body fluid Anatomy 0.000 description 1
239000010839 body fluid Substances 0.000 description 1
108010089934 carbohydrase Proteins 0.000 description 1
150000001720 carbohydrates Chemical class 0.000 description 1
239000003054 catalyst Substances 0.000 description 1
238000013375 chromatographic separation Methods 0.000 description 1
238000004587 chromatography analysis Methods 0.000 description 1
238000003776 cleavage reaction Methods 0.000 description 1
238000007796 conventional method Methods 0.000 description 1
230000006196 deacetylation Effects 0.000 description 1
238000003381 deacetylation reaction Methods 0.000 description 1
230000000694 effects Effects 0.000 description 1
238000006911 enzymatic reaction Methods 0.000 description 1
230000009229 glucose formation Effects 0.000 description 1
239000003112 inhibitor Substances 0.000 description 1
230000000977 initiatory effect Effects 0.000 description 1
230000003993 interaction Effects 0.000 description 1
238000004811 liquid chromatography Methods 0.000 description 1
238000005259 measurement Methods 0.000 description 1
XELZGAJCZANUQH-UHFFFAOYSA-N methyl 1-acetylthieno[3,2-c]pyrazole-5-carboxylate Chemical compound CC(=O)N1N=CC2=C1C=C(C(=O)OC)S2 XELZGAJCZANUQH-UHFFFAOYSA-N 0.000 description 1
239000000203 mixture Substances 0.000 description 1
230000001575 pathological effect Effects 0.000 description 1
229940031826 phenolate Drugs 0.000 description 1
238000002360 preparation method Methods 0.000 description 1
102000004169 proteins and genes Human genes 0.000 description 1
108090000623 proteins and genes Proteins 0.000 description 1
238000000746 purification Methods 0.000 description 1
230000036632 reaction speed Effects 0.000 description 1
230000009257 reactivity Effects 0.000 description 1
210000003079 salivary gland Anatomy 0.000 description 1
239000012488 sample solution Substances 0.000 description 1
230000007017 scission Effects 0.000 description 1
230000028327 secretion Effects 0.000 description 1
238000007086 side reaction Methods 0.000 description 1
229910001961 silver nitrate Inorganic materials 0.000 description 1
239000008107 starch Substances 0.000 description 1
235000019698 starch Nutrition 0.000 description 1
YNJBWRMUSHSURL-UHFFFAOYSA-N trichloroacetic acid Chemical compound OC(=O)C(Cl)(Cl)Cl YNJBWRMUSHSURL-UHFFFAOYSA-N 0.000 description 1

Classifications

- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/34—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving hydrolase
- C12Q1/40—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving hydrolase involving amylase
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q2334/00—O-linked chromogens for determinations of hydrolase enzymes, e.g. glycosidases, phosphatases, esterases
- C12Q2334/10—O-linked chromogens for determinations of hydrolase enzymes, e.g. glycosidases, phosphatases, esterases p-Nitrophenol derivatives

Landscapes

Chemical & Material Sciences (AREA)
Organic Chemistry (AREA)
Life Sciences & Earth Sciences (AREA)
Zoology (AREA)
Wood Science & Technology (AREA)
Proteomics, Peptides & Aminoacids (AREA)
Health & Medical Sciences (AREA)
Engineering & Computer Science (AREA)
Microbiology (AREA)
Biochemistry (AREA)
Physics & Mathematics (AREA)
Molecular Biology (AREA)
Biotechnology (AREA)
Biophysics (AREA)
Analytical Chemistry (AREA)
Immunology (AREA)
Bioinformatics & Cheminformatics (AREA)
General Engineering & Computer Science (AREA)
General Health & Medical Sciences (AREA)
Genetics & Genomics (AREA)
Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Saccharide Compounds (AREA)

CH864077A 1976-07-13 1977-07-12 Verfahren zur bestimmung des amylasegehalts von proben und mittel zur ausfuehrung des verfahrens. CH633582A5 (de)

Applications Claiming Priority (1)

Application Number	Priority Date	Filing Date	Title
US70497676A	1976-07-13	1976-07-13

Publications (1)

Publication Number	Publication Date
CH633582A5 true CH633582A5 (de)	1982-12-15

Family

ID=24831606

Family Applications (1)

Application Number	Title	Priority Date	Filing Date
CH864077A CH633582A5 (de)	1976-07-13	1977-07-12	Verfahren zur bestimmung des amylasegehalts von proben und mittel zur ausfuehrung des verfahrens.

Country Status (12)

Country	Link
JP (1)	JPS5311092A (fr)
BE (1)	BE856737A (fr)
CA (1)	CA1104912A (fr)
CH (1)	CH633582A5 (fr)
DE (1)	DE2731421A1 (fr)
DK (1)	DK155751C (fr)
FR (1)	FR2358660A1 (fr)
GB (1)	GB1571642A (fr)
IE (1)	IE45395B1 (fr)
IT (1)	IT1114891B (fr)
LU (1)	LU77751A1 (fr)
NL (1)	NL179399C (fr)

Families Citing this family (9)

* Cited by examiner, † Cited by third party
Publication number	Priority date	Publication date	Assignee	Title
US4102747A (en) *	1977-07-28	1978-07-25	American Hospital Supply Corporation	Amylase determination
AT362526B (de) *	1977-09-13	1981-05-25	Boehringer Mannheim Gmbh	Verfahren und reagens zur bestimmung von alpha- -amylase
DE2741192C2 (de) *	1977-09-13	1982-07-01	Boehringer Mannheim Gmbh, 6800 Mannheim	Verfahren zur Bestimmung von alpha- Amylase
DE2755803A1 (de) *	1977-12-14	1979-06-21	Boehringer Mannheim Gmbh	Verfahren und reagens zur bestimmung von alpha-amylase
DE2752501A1 (de) *	1977-11-24	1979-05-31	Kurt Prof Dr Wallenfels	Mit indikatorgruppen substituierte alpha-d-maltodextrine, verfahren zu deren herstellung und ihre verwendung zur bestimmung von glukosidasen und amylasen
JPS5768798A (en) *	1980-10-14	1982-04-27	Toyo Jozo Co Ltd	Novel measurement of amylase activity
DE3328616A1 (de) *	1983-08-08	1985-02-28	Boehringer Mannheim Gmbh, 6800 Mannheim	Oligoglucosidderivate
US4649108A (en) *	1984-07-26	1987-03-10	Genzyme Corporation	Alpha amylase assay
US4963479A (en) *	1986-10-07	1990-10-16	Hoechst Celanese Corporation	Reagent system for an alpha-amylase assay containing aromatic substituted glycoside

Family Cites Families (8)

* Cited by examiner, † Cited by third party
Publication number	Priority date	Publication date	Assignee	Title
US2094693A (en) *	1935-06-17	1937-10-05	Trojan Powder Co	Nitration of sugars and their glycosides
GB1167083A (en) *	1966-01-20	1969-10-15	Warner Lambert Pharmaceutical	Assay Method for Amylase
FR1508496A (fr) *	1966-01-20	1968-01-05	Warner Lambert Pharmaceutical	Produit et procédé pour le dosage de l'amylase
SE336915B (fr) *	1968-01-15	1971-07-19	Pharmacia Ab
CA973499A (en) *	1970-07-28	1975-08-26	Hayashibara, Ken	Process for the preparation of amylose as the substrate for the quantitative analysis of amylose
US3879263A (en) *	1973-09-06	1975-04-22	Du Pont	Method for the determination of amylase
JPS5185790A (en) *	1975-01-24	1976-07-27	Ono Pharmaceutical Co	Hitononyooyobitaiekinogurukoamiraazenobiryoteiryoho
CA1096376A (fr) *	1976-07-13	1981-02-24	William B. Farnham	Procede de preparation de glycosides nitroaromatiques

1977
- 1977-07-11 CA CA000282419A patent/CA1104912A/fr not_active Expired
- 1977-07-11 NL NL7707682A patent/NL179399C/xx not_active IP Right Cessation
- 1977-07-12 CH CH864077A patent/CH633582A5/de not_active IP Right Cessation
- 1977-07-12 IT IT2565377A patent/IT1114891B/it active
- 1977-07-12 DE DE19772731421 patent/DE2731421A1/de active Granted
- 1977-07-12 FR FR7721470A patent/FR2358660A1/fr active Granted
- 1977-07-12 DK DK315777A patent/DK155751C/da not_active IP Right Cessation
- 1977-07-12 BE BE179280A patent/BE856737A/fr not_active IP Right Cessation
- 1977-07-13 LU LU77751A patent/LU77751A1/xx unknown
- 1977-07-13 GB GB2950477A patent/GB1571642A/en not_active Expired
- 1977-07-13 JP JP8400177A patent/JPS5311092A/ja active Granted
- 1977-07-13 IE IE145577A patent/IE45395B1/en not_active IP Right Cessation

Also Published As

Publication number	Publication date
JPS5311092A (en)	1978-02-01
LU77751A1 (fr)	1978-02-02
BE856737A (fr)	1978-01-12
FR2358660A1 (fr)	1978-02-10
GB1571642A (en)	1980-07-16
JPS5753079B2 (fr)	1982-11-11
CA1104912A (fr)	1981-07-14
FR2358660B1 (fr)	1983-08-12
NL179399B (nl)	1986-04-01
NL7707682A (nl)	1978-01-17
DK315777A (da)	1978-01-14
IT1114891B (it)	1986-01-27
DE2731421C2 (fr)	1988-07-07
IE45395L (en)	1978-01-12
IE45395B1 (en)	1982-08-11
NL179399C (nl)	1986-09-01
DE2731421A1 (de)	1978-02-09
DK155751B (da)	1989-05-08
DK155751C (da)	1989-10-23

Legal Events

Date	Code	Title	Description
1994-03-31	PL	Patent ceased
2024-10-31	PL	Patent ceased

Publication	Publication Date	Title
DE3751253T2 (de)	1995-09-21	Mit Aromaten substituiertes Glykosid.
DE3208832C2 (fr)	1991-06-13
US4233403A (en)	1980-11-11	Amylase assay
DE68925420T2 (de)	1996-05-30	Limulus amoebozyt-lysat g-faktor-aktivierungs inhibitor
O'Brien	1977	Neuraminidase deficiency in the cherry red spot-myoclonus syndrome
EP0157384B1 (fr)	1995-01-18	Substrats pour hydrolases, leur méthode de préparation et leur emploi
EP0250991B1 (fr)	1993-01-27	Procédé pour déterminer la teneur en fructoseamine de sérum et mélange de réactifs approprié
EP0133329B1 (fr)	1990-02-28	Méthode et réactif pour la détermination d'alpha-amylase
CH651590A5 (de)	1985-09-30	Verfahren und reagensmischung zur bestimmung von alpha-amylase.
CH633582A5 (de)	1982-12-15	Verfahren zur bestimmung des amylasegehalts von proben und mittel zur ausfuehrung des verfahrens.
EP0146866B1 (fr)	1991-06-19	Phénylsulphonphtaleinyl-bêta-D-galactosides, leur méthode de préparation et leur emploi pour la détermination de la bêta-D-galactosidase
EP0031941A1 (fr)	1981-07-15	Indoxylmaltodextrines, procédé pour leur préparation et leur utilisation
DE69836496T2 (de)	2007-10-31	Verfahren zur herstellung von beta-(1,3)-d-glukan aus pilzen
DE2705859C2 (fr)	1988-09-08
DE1643196C2 (de)	1985-02-21	Wasserlösliche aus Stärke, Amylose oder Amylopektin und einem Farbstoff bestehende Verbindungen sowie Verfahren zu deren Herstellung
DE69619973T2 (de)	2002-11-07	Verwendung von beta-d-galactopyranosyl-d-xylosen zur herstellung von zusammensetzungen und lösungen, die zur bewertung der intestinalen lactoseaktivität bestimmt sind, und herstellungsverfahren
EP0034692B1 (fr)	1986-01-08	L'application des dérivés d'amedon pour les agents d'indication
DE69219855T2 (de)	1997-09-11	Verfahren zur Bestimmung der alpha-Amylase-Aktivität
DD233142A5 (de)	1986-02-19	Hybridoma-antikoerper
DE4338375A1 (de)	1994-06-23	Reagens zur Bestimmung der alpha-Amylaseaktivität und Verfahren zur Bestimmung der alpha-Amylaseaktivität
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DE69314844T2 (de)	1998-03-05	Stabiles, flüssiges Alpha-Amylase-Reagenz
EP0021310B1 (fr)	1983-07-20	Procédé et réactif pour la détermination du fructose