CL2009000193A1 - Vector para la transferencia de genes capaz de reproducirse en plantas que contienen genes del virus lr-2, celula vegetal que lo comprende, metodo de produccion de dicha construccion molecular, y procedimiento para expresar un gen heterologo en una celula vegetal. - Google Patents

Vector para la transferencia de genes capaz de reproducirse en plantas que contienen genes del virus lr-2, celula vegetal que lo comprende, metodo de produccion de dicha construccion molecular, y procedimiento para expresar un gen heterologo en una celula vegetal.

Info

Publication number
CL2009000193A1
CL2009000193A1 CL2009000193A CL2009000193A CL2009000193A1 CL 2009000193 A1 CL2009000193 A1 CL 2009000193A1 CL 2009000193 A CL2009000193 A CL 2009000193A CL 2009000193 A CL2009000193 A CL 2009000193A CL 2009000193 A1 CL2009000193 A1 CL 2009000193A1
Authority
CL
Chile
Prior art keywords
vector
plant cell
genes
virus
cell
Prior art date
Application number
CL2009000193A
Other languages
English (en)
Inventor
Valerian V Dolja
Valera V Peremyslov
Original Assignee
The State Of Oregon Acting By And Through The State Board Of Higher Education On Behalf Of Oregon St
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by The State Of Oregon Acting By And Through The State Board Of Higher Education On Behalf Of Oregon St filed Critical The State Of Oregon Acting By And Through The State Board Of Higher Education On Behalf Of Oregon St
Publication of CL2009000193A1 publication Critical patent/CL2009000193A1/es

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/005—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from viruses
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8216—Methods for controlling, regulating or enhancing expression of transgenes in plant cells
    • C12N15/8237—Externally regulated expression systems
    • C12N15/8239—Externally regulated expression systems pathogen inducible
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8201—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation
    • C12N15/8202—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation by biological means, e.g. cell mediated or natural vector
    • C12N15/8203—Virus mediated transformation
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2770/00—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA ssRNA viruses positive-sense
    • C12N2770/00011—Details
    • C12N2770/00022—New viral proteins or individual genes, new structural or functional aspects of known viral proteins or genes

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Biomedical Technology (AREA)
  • Organic Chemistry (AREA)
  • Biotechnology (AREA)
  • Molecular Biology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Zoology (AREA)
  • General Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Biophysics (AREA)
  • Virology (AREA)
  • Physics & Mathematics (AREA)
  • Cell Biology (AREA)
  • Microbiology (AREA)
  • Plant Pathology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Medicinal Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Communicable Diseases (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)

Abstract

UN VECTOR PARA LA EXPRESION DE GENES CAPAZ DE REPLICARSE EN VIDES QUE COMPRENDE UN ÁCIDO NUCLEICO QUE CODIFICA: A) GENES VIRALES DEL GRAPEVINE LEAFROL-ASSOCIATED VIRUS-2 (LR-2) COMPRENDIENDO UNA METILTRANSFERASA, UNA ARN HELICASA, Y UNA ARN POLMERASA DEPENDIENTE DE ARN; B) UN ARNI SUPRESOR SELECCIONADO DE) GRUPO FORMADO POR P24 DE LR-2 Y P21 DEL VIRUS AMARILLO DE LA REMOLACHA; C) PROTEASAS DIRECTRICES L1 Y L2 DE LR-2; Y D) UN POLINUCLEÁTIDO HETERÓLOGO UNIDO OPERATIVAMENTE A UN PROMOTOR. UNA CÉLULA DE VID QUE COMPRENDE DICHO VECTOR. UN MÉTODO PARA EXPRESAR UN GEN HETERÓLOGO EN UNA CÉLULA DE VID QUE COMPRENDE INTRODUCIR DICHO VECTOR EN LA CÉLULA DE VID. UN MÉTODO PARA PRODUCIR DICHO VECTOR QUE ADEMÁS COMPRENDE RECUPERAR E) VECTOR DE A CÉLULA DE VID O DEL MEDIO DONDE SE CULTIVA LA CÉLULA DE VID.
CL2009000193A 2008-01-31 2009-01-29 Vector para la transferencia de genes capaz de reproducirse en plantas que contienen genes del virus lr-2, celula vegetal que lo comprende, metodo de produccion de dicha construccion molecular, y procedimiento para expresar un gen heterologo en una celula vegetal. CL2009000193A1 (es)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US6330508P 2008-01-31 2008-01-31
US8350408P 2008-07-24 2008-07-24

Publications (1)

Publication Number Publication Date
CL2009000193A1 true CL2009000193A1 (es) 2009-08-21

Family

ID=40952630

Family Applications (1)

Application Number Title Priority Date Filing Date
CL2009000193A CL2009000193A1 (es) 2008-01-31 2009-01-29 Vector para la transferencia de genes capaz de reproducirse en plantas que contienen genes del virus lr-2, celula vegetal que lo comprende, metodo de produccion de dicha construccion molecular, y procedimiento para expresar un gen heterologo en una celula vegetal.

Country Status (9)

Country Link
US (2) US8415147B2 (es)
EP (1) EP2240580B1 (es)
CN (1) CN101978051A (es)
AR (1) AR070326A1 (es)
AU (1) AU2009210508B2 (es)
CL (1) CL2009000193A1 (es)
ES (1) ES2478041T3 (es)
WO (1) WO2009099877A2 (es)
ZA (1) ZA201005866B (es)

Families Citing this family (11)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US8389804B2 (en) 2010-04-08 2013-03-05 University Of Florida Research Foundation, Inc. Viral based transient-expression vector system that allows multiple applications
ES2477141B2 (es) * 2010-04-08 2015-02-23 University Of Florida Research Foundation, Inc. Sistema basado en un vector viral de expresión transitoria que permite múltiples aplicaciones
US20140056908A1 (en) * 2010-10-08 2014-02-27 Fraunhofer Usa Inc. Closterovirus-based nucleic acid molecules and uses thereof
CN105939599B (zh) * 2011-09-21 2019-10-18 佛罗里达大学研究基金会有限公司 使用柑橘衰退病毒载体进行外源基因表达
US10017747B2 (en) 2011-09-21 2018-07-10 University Of Florida Research Foundation, Inc. Citrus tristeza virus based vectors for foreign gene/s expression
CN103421839B (zh) * 2013-07-24 2015-04-29 河南农业大学 瓜类褪绿黄化病毒侵染性载体的构建方法
CN105063038B (zh) * 2015-09-01 2017-12-26 中华人民共和国伊犁出入境检验检疫局 一种检测葡萄卷叶伴随病毒3号的rpa引物及试剂盒
CN109777792B (zh) * 2018-09-29 2020-12-11 中国科学院动物研究所 一种rna解旋酶3及其编码基因和应用
CN110144338A (zh) * 2019-05-29 2019-08-20 通用生物系统（安徽）有限公司 一种以rna为模板的rna聚合酶制备方法及该聚合酶的应用
US20240090512A1 (en) * 2021-01-21 2024-03-21 Yissum Research Development Company Of The Hebrew University Of Jerusalem Ltd. Rnai nanoparticles and methods of using same in agriculture
CN117305267B (zh) * 2023-08-09 2024-05-28 中国农业大学 葡萄类受体蛋白激酶herk1的新用途

Family Cites Families (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6093544A (en) 1997-05-20 2000-07-25 Cornell Research Foundation, Inc. Rupestris stem pitting associated virus nucleic acids, proteins, and their uses
ZA984232B (en) * 1997-05-20 1998-12-01 Cornell Res Foundation Inc Grapevine leafroll virus type 2 proteins coding sequences and methods
WO2002033041A2 (en) 2000-10-18 2002-04-25 Large Scale Biology Corporation Production of bovine lysozyme by plant viral vectors
DE10109354A1 (de) * 2001-02-27 2002-09-05 Icon Genetics Ag Rekombinante virale Schaltersysteme
US20060228800A1 (en) 2003-05-15 2006-10-12 Shi-Lung Lin Novel Transgenic Methods Using intronic RNA

Also Published As

Publication number Publication date
AU2009210508A1 (en) 2009-08-13
EP2240580A2 (en) 2010-10-20
ES2478041T3 (es) 2014-07-18
WO2009099877A2 (en) 2009-08-13
US20130133109A1 (en) 2013-05-23
US20100333230A1 (en) 2010-12-30
US8415147B2 (en) 2013-04-09
AU2009210508B2 (en) 2015-04-09
EP2240580A4 (en) 2011-09-07
EP2240580B1 (en) 2014-04-30
AR070326A1 (es) 2010-03-31
CN101978051A (zh) 2011-02-16
ZA201005866B (en) 2011-11-30
WO2009099877A3 (en) 2010-01-07

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