CL2009000210A1 - Acidos nucleicos que comprenden una secuencia que codifica un peptido de transito a plastidios de una glaucosafita enlazada a una segunda secuencia que codifica un peptido heterologo; metodo para la expresion estable de un polipeptido heterologo en una planta; polipeptido aislado codificado por el acido nucleico descrito anteriormente. - Google Patents

Acidos nucleicos que comprenden una secuencia que codifica un peptido de transito a plastidios de una glaucosafita enlazada a una segunda secuencia que codifica un peptido heterologo; metodo para la expresion estable de un polipeptido heterologo en una planta; polipeptido aislado codificado por el acido nucleico descrito anteriormente.

Info

Publication number
CL2009000210A1
CL2009000210A1 CL2009000210A CL2009000210A CL2009000210A1 CL 2009000210 A1 CL2009000210 A1 CL 2009000210A1 CL 2009000210 A CL2009000210 A CL 2009000210A CL 2009000210 A CL2009000210 A CL 2009000210A CL 2009000210 A1 CL2009000210 A1 CL 2009000210A1
Authority
CL
Chile
Prior art keywords
sequence encoding
heterologous
peptide
glaucosaphyte
linked
Prior art date
Application number
CL2009000210A
Other languages
English (en)
Inventor
Stacy Miles
Gregory W Warren
John Hipskind
Original Assignee
Syngenta Participations Ag
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Syngenta Participations Ag filed Critical Syngenta Participations Ag
Publication of CL2009000210A1 publication Critical patent/CL2009000210A1/es

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8242—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits
    • C12N15/8257—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits for the production of primary gene products, e.g. pharmaceutical products, interferon
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8216—Methods for controlling, regulating or enhancing expression of transgenes in plant cells
    • C12N15/8221—Transit peptides
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8242—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits
    • C12N15/8243—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine
    • C12N15/8245—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine involving modified carbohydrate or sugar alcohol metabolism, e.g. starch biosynthesis
    • C12N15/8246—Non-starch polysaccharides, e.g. cellulose, fructans, levans
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
    • C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
    • C12N9/2405—Glucanases
    • C12N9/2434—Glucanases acting on beta-1,4-glucosidic bonds
    • C12N9/2437—Cellulases (3.2.1.4; 3.2.1.74; 3.2.1.91; 3.2.1.150)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y302/00—Hydrolases acting on glycosyl compounds, i.e. glycosylases (3.2)
    • C12Y302/01—Glycosidases, i.e. enzymes hydrolysing O- and S-glycosyl compounds (3.2.1)
    • C12Y302/01091—Cellulose 1,4-beta-cellobiosidase (3.2.1.91)

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Biotechnology (AREA)
  • Molecular Biology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Physics & Mathematics (AREA)
  • Cell Biology (AREA)
  • Medicinal Chemistry (AREA)
  • Nutrition Science (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Peptides Or Proteins (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Fodder In General (AREA)

Abstract

Acido nucleico que comprende una secuencia que codifica un péptido de tránsito a plástidos de una glaucosafita enlazada a una segunda secuencia que codifica un péptido heterólogo; método para la expresión estable de un polipéptido heterólogo en una planta; polipéptido aislado que comprende el péptido de tránsito y heterólogo.
CL2009000210A 2008-01-30 2009-01-30 Acidos nucleicos que comprenden una secuencia que codifica un peptido de transito a plastidios de una glaucosafita enlazada a una segunda secuencia que codifica un peptido heterologo; metodo para la expresion estable de un polipeptido heterologo en una planta; polipeptido aislado codificado por el acido nucleico descrito anteriormente. CL2009000210A1 (es)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US2484408P 2008-01-30 2008-01-30
US4769208P 2008-04-24 2008-04-24

Publications (1)

Publication Number Publication Date
CL2009000210A1 true CL2009000210A1 (es) 2009-06-05

Family

ID=40386114

Family Applications (2)

Application Number Title Priority Date Filing Date
CL2009000211A CL2009000211A1 (es) 2008-01-30 2009-01-30 Molécula de ácido nucleico que comprende una secuencia de direccionamiento a vacuola de almacenamiento de un polipéptido heterologo; y procedimiento para la acumulacion de dichas proteinas en una planta.
CL2009000210A CL2009000210A1 (es) 2008-01-30 2009-01-30 Acidos nucleicos que comprenden una secuencia que codifica un peptido de transito a plastidios de una glaucosafita enlazada a una segunda secuencia que codifica un peptido heterologo; metodo para la expresion estable de un polipeptido heterologo en una planta; polipeptido aislado codificado por el acido nucleico descrito anteriormente.

Family Applications Before (1)

Application Number Title Priority Date Filing Date
CL2009000211A CL2009000211A1 (es) 2008-01-30 2009-01-30 Molécula de ácido nucleico que comprende una secuencia de direccionamiento a vacuola de almacenamiento de un polipéptido heterologo; y procedimiento para la acumulacion de dichas proteinas en una planta.

Country Status (7)

Country Link
US (2) US20090205075A1 (es)
CN (2) CN101960011A (es)
AR (2) AR070277A1 (es)
AU (1) AU2009210590A1 (es)
BR (2) BRPI0907621A2 (es)
CL (2) CL2009000211A1 (es)
WO (2) WO2009099749A1 (es)

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US20130071884A1 (en) 2009-11-06 2013-03-21 Agrivida, Inc. Plants expressing cell wall degrading enzymes and expression vectors
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Also Published As

Publication number Publication date
CN101960013A (zh) 2011-01-26
BRPI0906430A2 (pt) 2015-08-18
WO2009099783A2 (en) 2009-08-13
WO2009099749A1 (en) 2009-08-13
AR070277A1 (es) 2010-03-25
CN101960011A (zh) 2011-01-26
AU2009210590A1 (en) 2009-08-13
AR070314A1 (es) 2010-03-31
US8168863B2 (en) 2012-05-01
BRPI0907621A2 (pt) 2019-01-15
WO2009099783A3 (en) 2009-10-01
US20090193541A1 (en) 2009-07-30
US20090205075A1 (en) 2009-08-13
CL2009000211A1 (es) 2009-06-05

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