CL2011003084A1 - Celula cultivada que comprende un cristal de proteina formado mediante una pluralidad de un polipeptido de fusion que comprende una proteina cry fusionado a un polipeptido heterologo; cristal de proteina aislado de bacteria; acido nucleico y polipeptido que codifican polipeptido de fusion; metodo para aislar cristal de proteina. - Google Patents

Celula cultivada que comprende un cristal de proteina formado mediante una pluralidad de un polipeptido de fusion que comprende una proteina cry fusionado a un polipeptido heterologo; cristal de proteina aislado de bacteria; acido nucleico y polipeptido que codifican polipeptido de fusion; metodo para aislar cristal de proteina.

Info

Publication number
CL2011003084A1
CL2011003084A1 CL2011003084A CL2011003084A CL2011003084A1 CL 2011003084 A1 CL2011003084 A1 CL 2011003084A1 CL 2011003084 A CL2011003084 A CL 2011003084A CL 2011003084 A CL2011003084 A CL 2011003084A CL 2011003084 A1 CL2011003084 A1 CL 2011003084A1
Authority
CL
Chile
Prior art keywords
polypeptide
protein crystal
protein
fusion polypeptide
bacteria
Prior art date
Application number
CL2011003084A
Other languages
English (en)
Inventor
Michael K Chan
Manoj S Nair
Marianne M Lee
Original Assignee
Univ Ohio State Res Found
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Univ Ohio State Res Found filed Critical Univ Ohio State Res Found
Publication of CL2011003084A1 publication Critical patent/CL2011003084A1/es

Links

Classifications

    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K39/00—Medicinal preparations containing antigens or antibodies
    • A61K39/39—Medicinal preparations containing antigens or antibodies characterised by the immunostimulating additives, e.g. chemical adjuvants
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P37/00—Drugs for immunological or allergic disorders
    • A61P37/02—Immunomodulators
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P37/00—Drugs for immunological or allergic disorders
    • A61P37/02—Immunomodulators
    • A61P37/04—Immunostimulants
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/195—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria
    • C07K14/32—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria from Bacillus (G)
    • C07K14/325—Bacillus thuringiensis crystal peptides, i.e. delta-endotoxins
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/62—DNA sequences coding for fusion proteins
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K39/00—Medicinal preparations containing antigens or antibodies
    • A61K2039/555—Medicinal preparations containing antigens or antibodies characterised by a specific combination antigen/adjuvant
    • A61K2039/55511—Organic adjuvants
    • A61K2039/55544—Bacterial toxins
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/60—Fusion polypeptide containing spectroscopic/fluorescent detection, e.g. green fluorescent protein [GFP]
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/70—Fusion polypeptide containing domain for protein-protein interaction
    • C07K2319/735—Fusion polypeptide containing domain for protein-protein interaction containing a domain for self-assembly, e.g. a viral coat protein (includes phage display)

Landscapes

  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Immunology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Molecular Biology (AREA)
  • Medicinal Chemistry (AREA)
  • Biomedical Technology (AREA)
  • Biophysics (AREA)
  • Wood Science & Technology (AREA)
  • Public Health (AREA)
  • Veterinary Medicine (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Biochemistry (AREA)
  • General Chemical & Material Sciences (AREA)
  • Animal Behavior & Ethology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Microbiology (AREA)
  • Physics & Mathematics (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • Mycology (AREA)
  • Epidemiology (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Plant Pathology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Peptides Or Proteins (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
  • Medicines Containing Antibodies Or Antigens For Use As Internal Diagnostic Agents (AREA)
  • Agricultural Chemicals And Associated Chemicals (AREA)

Abstract

Célula cultivada que comprende un cristal de proteína formado mediante una pluralidad de un polipéptido de fusión que comprende una proteína cry fusionado a un polipéptido heterólogo; cristal de proteína aislado de bacteria; ácido nucleico y polipéptido que codifican polipéptido de fusión; método para aislar cristal de proteína.
CL2011003084A 2009-06-05 2011-12-05 Celula cultivada que comprende un cristal de proteina formado mediante una pluralidad de un polipeptido de fusion que comprende una proteina cry fusionado a un polipeptido heterologo; cristal de proteina aislado de bacteria; acido nucleico y polipeptido que codifican polipeptido de fusion; metodo para aislar cristal de proteina. CL2011003084A1 (es)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US18463709P 2009-06-05 2009-06-05
US31352510P 2010-03-12 2010-03-12

Publications (1)

Publication Number Publication Date
CL2011003084A1 true CL2011003084A1 (es) 2012-06-08

Family

ID=43298592

Family Applications (1)

Application Number Title Priority Date Filing Date
CL2011003084A CL2011003084A1 (es) 2009-06-05 2011-12-05 Celula cultivada que comprende un cristal de proteina formado mediante una pluralidad de un polipeptido de fusion que comprende una proteina cry fusionado a un polipeptido heterologo; cristal de proteina aislado de bacteria; acido nucleico y polipeptido que codifican polipeptido de fusion; metodo para aislar cristal de proteina.

Country Status (6)

Country Link
US (1) US20100322977A1 (es)
EP (1) EP2438173A4 (es)
JP (1) JP2012529269A (es)
CA (1) CA2763492A1 (es)
CL (1) CL2011003084A1 (es)
WO (1) WO2010141953A2 (es)

Families Citing this family (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2013085540A2 (en) * 2011-12-09 2013-06-13 The Ohio State University Research Foundation Cry crystals for the production of antimicrobial proteins
CN104498501B (zh) * 2014-12-05 2017-07-18 江苏省农业科学院 一种人源杀虫基因及其编码杀虫肽与应用
AR105155A1 (es) * 2015-07-07 2017-09-13 Syngenta Participations Ag Composiciones y métodos para controlar plagas de plantas
EP3956350B1 (en) 2019-04-16 2024-11-27 The Chinese University Of Hong Kong Engineered cry proteins for delivery of therapeutics
US12404308B2 (en) 2019-04-26 2025-09-02 The Chinese University Of Hong Kong In vivo immobilization of proteins
GB201917381D0 (en) * 2019-11-28 2020-01-15 Cell Guidance Systems Ltd Phagocytosed self-assembling proteins
FR3135094B1 (fr) * 2022-05-02 2025-02-28 Institut National De Rech Pour Lagriculture Lalimentation Et Lenvironnement Système d’expression bactérien de protéines hétérologues
EP4644408A1 (en) 2024-04-19 2025-11-05 The Chinese University of Hong Kong Office of Research and Knowledge Transfer Services (ORKTS) Polyethylenimine-modified cry proteins and their use

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US5254799A (en) * 1985-01-18 1993-10-19 Plant Genetic Systems N.V. Transformation vectors allowing expression of Bacillus thuringiensis endotoxins in plants
AP9901541A0 (en) * 1996-11-20 1999-06-30 Ecogen Inc Broad-spectrum delta-endotoxins.
US7157089B1 (en) * 1996-11-26 2007-01-02 Stressgen Biotechnologies Corporation Immune responses using compositions containing stress proteins
KR100264953B1 (ko) * 1998-04-03 2001-03-02 박현우 재조합 베큘로바이러스, 이의 제조방법 및 이를 포함하는 미생물 살충제
GB9909796D0 (en) * 1999-04-28 1999-06-23 Plant Bioscience Ltd Pesticidal fumes
ATE316980T1 (de) * 1999-07-21 2006-02-15 Univ Southern California Fusions-polypeptide zur gewebegeneration und wundheilung zur gezielten matrixanwendung
AU2001259260A1 (en) * 2000-05-15 2001-11-26 Monsanto Technology Llc Polypeptide composionns toxic to anthonomus insects, and use thereof
CN1484700A (zh) * 2000-11-02 2004-03-24 �¼��¹�����ѧ aopB基因,蛋白,同系物,片段和它们的变体,以及它们在细胞表面展示方面的应用
EP1988099B1 (en) * 2001-01-09 2012-11-14 Bayer CropScience NV Bacillus thuringiensis insecticidal proteins
US20050106625A1 (en) * 2003-09-26 2005-05-19 Biohesion Incorporated Recombinant fusion proteins with high affinity binding to gold and applications thereof
KR101225918B1 (ko) * 2004-02-25 2013-01-24 파이어니어 하이 부렛드 인터내쇼날 인코포레이팃드 신규한 바실루스 투링기엔시스 결정 폴리펩티드,폴리뉴클레오티드, 및 이의 조성물
US7723063B2 (en) * 2004-04-28 2010-05-25 Intrinsic Lifesciences Methods for measuring levels of bioactive human hepcidin
US8617838B2 (en) * 2004-09-20 2013-12-31 University Of Massachusetts Fluorescent proteins and related methods and compounds
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EA200970559A1 (ru) * 2006-12-08 2009-12-30 Пайонир Хай-Бред Интернэшнл, Инк. Новые кристаллические полипептиды из bacillus thuringiensis, кодирующие их полинуклеотиды и композиции этих соединений
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EP2187973A2 (en) * 2007-08-13 2010-05-26 Sea Lane Biotechnologies,llc. Spore displaying antigens

Also Published As

Publication number Publication date
CA2763492A1 (en) 2010-12-09
US20100322977A1 (en) 2010-12-23
WO2010141953A2 (en) 2010-12-09
EP2438173A4 (en) 2012-10-31
EP2438173A2 (en) 2012-04-11
JP2012529269A (ja) 2012-11-22
WO2010141953A3 (en) 2011-04-21

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