CL2012001353A1 - Método de detección de un evento de soja en una muestra basada en poner en contacto dicha muestra con un polinucleótido específico para el evento aad-12 pdab4468-0416; método para determinar cigocidad de dicho evento en una planta de soja y kit de detección de adn. - Google Patents

Método de detección de un evento de soja en una muestra basada en poner en contacto dicha muestra con un polinucleótido específico para el evento aad-12 pdab4468-0416; método para determinar cigocidad de dicho evento en una planta de soja y kit de detección de adn.

Info

Publication number
CL2012001353A1
CL2012001353A1 CL2012001353A CL2012001353A CL2012001353A1 CL 2012001353 A1 CL2012001353 A1 CL 2012001353A1 CL 2012001353 A CL2012001353 A CL 2012001353A CL 2012001353 A CL2012001353 A CL 2012001353A CL 2012001353 A1 CL2012001353 A1 CL 2012001353A1
Authority
CL
Chile
Prior art keywords
event
sample
soybean
pdab4468
contacting
Prior art date
Application number
CL2012001353A
Other languages
English (en)
Inventor
Stephen Novak
Yunxing Cory Cui
Thomas Greene
Ning Zhou
Original Assignee
Dow Agrosciences Llc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Dow Agrosciences Llc filed Critical Dow Agrosciences Llc
Publication of CL2012001353A1 publication Critical patent/CL2012001353A1/es

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • C12Q1/6888—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms
    • C12Q1/6895—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms for plants, fungi or algae
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8261—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield
    • C12N15/8271—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance
    • C12N15/8274—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance for herbicide resistance
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6816—Hybridisation assays characterised by the detection means
    • C12Q1/6818—Hybridisation assays characterised by the detection means involving interaction of two or more labels, e.g. resonant energy transfer
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2545/00—Reactions characterised by their quantitative nature
    • C12Q2545/10—Reactions characterised by their quantitative nature the purpose being quantitative analysis
    • C12Q2545/114—Reactions characterised by their quantitative nature the purpose being quantitative analysis involving a quantitation step
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2561/00—Nucleic acid detection characterised by assay method
    • C12Q2561/101—Taqman
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2565/00—Nucleic acid analysis characterised by mode or means of detection
    • C12Q2565/10—Detection mode being characterised by the assay principle
    • C12Q2565/101—Interaction between at least two labels
    • C12Q2565/1015—Interaction between at least two labels labels being on the same oligonucleotide
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2600/00—Oligonucleotides characterized by their use
    • C12Q2600/16—Primer sets for multiplex assays
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2600/00—Oligonucleotides characterized by their use
    • C12Q2600/166—Oligonucleotides used as internal standards, controls or normalisation probes

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Biotechnology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Analytical Chemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • Physics & Mathematics (AREA)
  • General Health & Medical Sciences (AREA)
  • Biophysics (AREA)
  • Microbiology (AREA)
  • Biomedical Technology (AREA)
  • Immunology (AREA)
  • Plant Pathology (AREA)
  • Cell Biology (AREA)
  • Botany (AREA)
  • Mycology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

Método de detección de un evento de soja en una muestra que comprende ADN de soja que comprende poner en contacto dicha muestra con un polinucleótido específico para el evento AAD-12 pDAB4468-0416; método para determinar cigocidad de dicho evento en una planta de soja; y kit de detección de ADN.
CL2012001353A 2009-11-24 2012-05-24 Método de detección de un evento de soja en una muestra basada en poner en contacto dicha muestra con un polinucleótido específico para el evento aad-12 pdab4468-0416; método para determinar cigocidad de dicho evento en una planta de soja y kit de detección de adn. CL2012001353A1 (es)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US26395009P 2009-11-24 2009-11-24
US32736910P 2010-04-23 2010-04-23

Publications (1)

Publication Number Publication Date
CL2012001353A1 true CL2012001353A1 (es) 2012-10-05

Family

ID=44212274

Family Applications (1)

Application Number Title Priority Date Filing Date
CL2012001353A CL2012001353A1 (es) 2009-11-24 2012-05-24 Método de detección de un evento de soja en una muestra basada en poner en contacto dicha muestra con un polinucleótido específico para el evento aad-12 pdab4468-0416; método para determinar cigocidad de dicho evento en una planta de soja y kit de detección de adn.

Country Status (16)

Country Link
US (1) US8685677B2 (es)
EP (1) EP2504456B1 (es)
JP (1) JP5907885B2 (es)
KR (1) KR20120107974A (es)
CN (1) CN102782153B (es)
AR (1) AR079150A1 (es)
AU (1) AU2010324818B2 (es)
CA (1) CA2781622A1 (es)
CL (1) CL2012001353A1 (es)
CO (1) CO6561770A2 (es)
IL (1) IL219932A0 (es)
IN (1) IN2012DN04901A (es)
MX (1) MX2012006074A (es)
RU (1) RU2573898C2 (es)
UY (1) UY33058A (es)
ZA (1) ZA201203713B (es)

Families Citing this family (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2011066384A1 (en) 2009-11-24 2011-06-03 Dow Agrosciences Llc Aad-12 event 416, related transgenic soybean lines, and event-specific identification thereof
WO2016054071A1 (en) 2014-09-29 2016-04-07 Alforex Seeds LLC Low lignin non-transgenic alfalfa varieties and methods for producing the same
WO2018089099A1 (en) 2016-10-11 2018-05-17 Dow Agrosciences Llc Modulation of transgene expression in plants
CN116144671B (zh) * 2022-09-23 2025-07-11 隆平生物技术(海南)有限公司 转基因玉米事件lp026-3及其检测方法
CN116676417B (zh) * 2023-07-24 2023-09-29 捷康生物科技(海南)有限公司 转基因大豆事件jk1001-3及其检测方法
CN116694813B (zh) * 2023-07-25 2023-10-03 隆平生物技术(海南)有限公司 转基因大豆事件lp086-1及其检测方法
CN116694812B (zh) * 2023-07-25 2023-10-03 隆平生物技术(海南)有限公司 转基因大豆事件lp086-2及其检测方法
CN116694814B (zh) * 2023-08-01 2023-10-10 隆平生物技术(海南)有限公司 转基因大豆事件lp012-1及其检测方法
CN116694815B (zh) * 2023-08-01 2023-10-03 隆平生物技术(海南)有限公司 转基因大豆事件lp012-2及其检测方法

Family Cites Families (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20060127889A1 (en) 2000-07-25 2006-06-15 Dotson Stanton B Method for assessing transgene expression and copy number
CN1370841A (zh) * 2001-02-26 2002-09-25 安利忻 基因改造农作物及其加工产品的定量检测方法和试剂盒
US7955800B2 (en) * 2002-06-25 2011-06-07 Advpharma Inc. Metastasis-associated gene profiling for identification of tumor tissue, subtyping, and prediction of prognosis of patients
RU2270254C2 (ru) * 2004-04-30 2006-02-20 Институт Молекулярной Биологии Им. В.А. Энгельгардта Российской Академии Наук Способ идентификации трансгенных последовательностей днк в растительном материале и продуктах на его основе, набор олигонуклеотидов и биочип для осуществления этого способа
NZ608188A (en) * 2005-10-28 2014-10-31 Dow Agrosciences Llc Novel herbicide resistance genes
WO2011066384A1 (en) * 2009-11-24 2011-06-03 Dow Agrosciences Llc Aad-12 event 416, related transgenic soybean lines, and event-specific identification thereof
US8071741B2 (en) * 2007-04-20 2011-12-06 Defiante Farmaceutica, S.A. Stable recombinant adenosine deaminase

Also Published As

Publication number Publication date
AU2010324818B2 (en) 2015-09-17
IL219932A0 (en) 2012-07-31
CO6561770A2 (es) 2012-11-15
IN2012DN04901A (es) 2015-09-25
UY33058A (es) 2011-06-30
EP2504456A1 (en) 2012-10-03
EP2504456B1 (en) 2017-12-20
CN102782153A (zh) 2012-11-14
CA2781622A1 (en) 2011-06-03
MX2012006074A (es) 2012-06-19
KR20120107974A (ko) 2012-10-04
AU2010324818A1 (en) 2012-06-14
ZA201203713B (en) 2013-01-30
CN102782153B (zh) 2015-08-12
AR079150A1 (es) 2011-12-28
EP2504456A4 (en) 2014-04-09
RU2012126088A (ru) 2013-12-27
RU2573898C2 (ru) 2016-01-27
JP2013512661A (ja) 2013-04-18
JP5907885B2 (ja) 2016-04-26
US8685677B2 (en) 2014-04-01
US20130029329A1 (en) 2013-01-31

Similar Documents

Publication Publication Date Title
CR20110567A (es) Dispositivos para diagnostico y metodos relacionados
MX373248B (es) Prediccion de riesgo de evento cardiovascular y usos del mismo.
CL2013001717A1 (es) Molecula de acido nucleico que codifica un polipeptido con actividad enzimatica de una enzima hidroxifenilpiruvato dioxigenasa (hppd); metodo de produccion de una planta de soja resistente a un inhibidor de hppd y/o glufosinato; metodo de control de malezas; metodo para identificar el evento syht0h2 en una muestra; y kit de deteccion.
WO2011116006A3 (en) A rapid method to measure cyanide in biological samples
CL2011002973A1 (es) Metodo para detectar anticuerpos en una muestra que comprende contactar una muestra con un soporte que tiene fijado un peptoide; kit para detectar anticuerpos que son indicativos de una enfermedad neurodegenerativa.
WO2011065666A3 (en) Centrifugal micro-fluidic device and method for detecting target in fluid sample
CL2011002228A1 (es) Aparato para el analisis integrado de acidos nucleicos en tiempo real, y metodo de detecicion de dichas moleculas.
EP2576833A4 (en) METHOD AND DEVICE FOR THE POINT-OF-CARE DETECTION OF NUCLEIC ACID IN A SAMPLE
CL2013003605A1 (es) Metodo para la evaluacion del riesgo de un evento tromboembólico en un sujeto que comprende determinar la presencia de serpin a 10 (inhibidor de la proteina z); kit que comprende reactivos para detectar serpin a 10.
NZ605698A (en) Methods and compositions for diagnosis and prognosis of renal injury and renal failure
ES2364314T8 (es) Detección y predicción de preeclampsia.
PE20141450A1 (es) Ensayo de lisiloxidasa-homologo 2 y metodos de uso del mismo
WO2011087789A3 (en) Methods for the detection of microorganisms
UY33058A (es) Detección del evento de soja aad-12 416
AR059361A1 (es) Dispositivo y metodos para detectar y cuantificar uno o mas agentes objetivos
EA201171178A1 (ru) Устройство и способ для определения и количественного анализа аналитов, в частности, микотоксинов
WO2012025637A9 (en) An agglutination assay method and device
MX2009004852A (es) Procedimiento para la determinacion de la fragmentacion del adn en microorganismos.
CL2008001621A1 (es) Metodo para detectar el componente caliqueamicina en un conjugado covalente caliqueamicina-portador en una muestra de fluido, donde la caliqueamicina se detecta luego de romper dicha union, preferentemente por espectrometria de masas; kit para determinar la concentracion de caliqueamicina en una muestra.
BRPI1009573A2 (pt) método para avaliar uma condição artrítica em um sujeito, kit para detectar a presença de um auto-anticorpo, e, método para determinar o subtipo de artrite em um paciente.
WO2012145516A3 (en) Methods and kits for quantitative determination of total organic acid content in a coolant
MA35427B1 (fr) Immunoessai permettant de detecter des antibiotiques
MX2012000041A (es) Metodo para someter a prueba a un sujeto que se cree esta predispuesto a tener cancer metastasico usando delta133p53beta.
DK2418486T3 (da) Testreagenskit og dets anvendelse i en fremgangsmåde til måling af en analyt i testprøve
CL2011003063A1 (es) Metodo de alto rendimiento para la deteccion de la presencia de dos o mas proteinas de interes con secuencias de aminoacidos conocidas en una muestra de planta, y procedimiento para mantener el genotipo de una variedad de la planta transgenica.