CL2014003419A1 - Célula microbiana recombinante que sobreexpresa una secuencia que codifica acil-coa: lisofosfatidilcolina aciltranferasa y fosofolípido: diacilglicerol aciltransferasas; y método de producción mejorada de ácidos grasos poliinsaturados. - Google Patents

Célula microbiana recombinante que sobreexpresa una secuencia que codifica acil-coa: lisofosfatidilcolina aciltranferasa y fosofolípido: diacilglicerol aciltransferasas; y método de producción mejorada de ácidos grasos poliinsaturados.

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Publication number
CL2014003419A1
CL2014003419A1 CL2014003419A CL2014003419A CL2014003419A1 CL 2014003419 A1 CL2014003419 A1 CL 2014003419A1 CL 2014003419 A CL2014003419 A CL 2014003419A CL 2014003419 A CL2014003419 A CL 2014003419A CL 2014003419 A1 CL2014003419 A1 CL 2014003419A1
Authority
CL
Chile
Prior art keywords
acyltranferase
lysophosphatidylcholine
overexpresses
coa
phospholipid
Prior art date
Application number
CL2014003419A
Other languages
English (en)
Inventor
Michael W Bostick
Hongxiang Zhang
Quinn Qun Zhu
Narandra S Yadav
Original Assignee
Du Pont
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Du Pont filed Critical Du Pont
Publication of CL2014003419A1 publication Critical patent/CL2014003419A1/es

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/64—Fats; Fatty oils; Ester-type waxes; Higher fatty acids, i.e. having at least seven carbon atoms in an unbroken chain bound to a carboxyl group; Oxidised oils or fats
    • C12P7/6409—Fatty acids
    • C12P7/6427—Polyunsaturated fatty acids [PUFA], i.e. having two or more double bonds in their backbone
    • C12P7/6432—Eicosapentaenoic acids [EPA]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/80—Vectors or expression systems specially adapted for eukaryotic hosts for fungi
    • C12N15/81—Vectors or expression systems specially adapted for eukaryotic hosts for fungi for yeasts
    • C12N15/815—Vectors or expression systems specially adapted for eukaryotic hosts for fungi for yeasts for yeasts other than Saccharomyces
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/1025—Acyltransferases (2.3)
    • C12N9/1029—Acyltransferases (2.3) transferring groups other than amino-acyl groups (2.3.1)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/64—Fats; Fatty oils; Ester-type waxes; Higher fatty acids, i.e. having at least seven carbon atoms in an unbroken chain bound to a carboxyl group; Oxidised oils or fats
    • C12P7/6409—Fatty acids
    • C12P7/6427—Polyunsaturated fatty acids [PUFA], i.e. having two or more double bonds in their backbone
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
    • Y02P20/00—Technologies relating to chemical industry
    • Y02P20/50—Improvements relating to the production of bulk chemicals
    • Y02P20/52—Improvements relating to the production of bulk chemicals using catalysts, e.g. selective catalysts

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Health & Medical Sciences (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Genetics & Genomics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • Biomedical Technology (AREA)
  • Oil, Petroleum & Natural Gas (AREA)
  • General Chemical & Material Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Molecular Biology (AREA)
  • Mycology (AREA)
  • Medicinal Chemistry (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Plant Pathology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Enzymes And Modification Thereof (AREA)
CL2014003419A 2012-06-19 2014-12-17 Célula microbiana recombinante que sobreexpresa una secuencia que codifica acil-coa: lisofosfatidilcolina aciltranferasa y fosofolípido: diacilglicerol aciltransferasas; y método de producción mejorada de ácidos grasos poliinsaturados. CL2014003419A1 (es)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US201261661623P 2012-06-19 2012-06-19
US201261661615P 2012-06-19 2012-06-19

Publications (1)

Publication Number Publication Date
CL2014003419A1 true CL2014003419A1 (es) 2015-08-28

Family

ID=48670865

Family Applications (2)

Application Number Title Priority Date Filing Date
CL2014003418A CL2014003418A1 (es) 2012-06-19 2014-12-17 Polinucleótido que codifica polipéptido mutante con actividad acil-coa: lisofosfatidilcolina aciltransferasa; célula recombinante que lo comprende; constructo; célula transformada que produce al menos un ácido graso poliinsaturado de cadena larga.
CL2014003419A CL2014003419A1 (es) 2012-06-19 2014-12-17 Célula microbiana recombinante que sobreexpresa una secuencia que codifica acil-coa: lisofosfatidilcolina aciltranferasa y fosofolípido: diacilglicerol aciltransferasas; y método de producción mejorada de ácidos grasos poliinsaturados.

Family Applications Before (1)

Application Number Title Priority Date Filing Date
CL2014003418A CL2014003418A1 (es) 2012-06-19 2014-12-17 Polinucleótido que codifica polipéptido mutante con actividad acil-coa: lisofosfatidilcolina aciltransferasa; célula recombinante que lo comprende; constructo; célula transformada que produce al menos un ácido graso poliinsaturado de cadena larga.

Country Status (8)

Country Link
US (2) US9347075B2 (es)
EP (2) EP2861727B1 (es)
JP (2) JP6351582B2 (es)
BR (2) BR112014031784A2 (es)
CA (2) CA2877279A1 (es)
CL (2) CL2014003418A1 (es)
DK (2) DK2861728T3 (es)
WO (2) WO2013192002A1 (es)

Families Citing this family (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2013192002A1 (en) * 2012-06-19 2013-12-27 E. I. Du Pont De Nemours And Company IMPROVED PRODUCTION OF POLYUNSATURATED FATTY ACIDS BY COEXPRESSION OF ACYL-CoA:LYSOPHOSPHATIDYLCHOLINE ACYLTRANSFERASES AND PHOSPHOLIPID:DIACYLGLYCEROL ACYLTRANSFERASES
JP2016502852A (ja) * 2012-12-21 2016-02-01 イー・アイ・デュポン・ドウ・ヌムール・アンド・カンパニーＥ．Ｉ．Ｄｕ Ｐｏｎｔ Ｄｅ Ｎｅｍｏｕｒｓ Ａｎｄ Ｃｏｍｐａｎｙ 乾燥細胞重量で少なくとも２８％のエイコサペンタエン酸を産生する組換え微生物細胞
JP2017195777A (ja) * 2014-08-29 2017-11-02 国立大学法人京都大学 エイコサペンタエン酸を高含有する脂質の生産方法
CN108330114B (zh) * 2018-03-21 2020-11-03 北京大学 一种利用epa的甘油二酯酰基转移酶及其应用
CN115820586B (zh) * 2022-09-06 2025-07-22 中国农业科学院油料作物研究所 一种溶血磷脂酰胆碱酰基转移酶、核酸分子及其应用

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US5071764A (en) 1983-10-06 1991-12-10 Pfizer Inc. Process for integrative transformation of yarrowia lipolytica
US4683202A (en) 1985-03-28 1987-07-28 Cetus Corporation Process for amplifying nucleic acid sequences
EP1598422B1 (en) 1997-04-11 2013-01-02 Calgene LLC Methods and compositions for synthesis of long chain polyunsaturated fatty acids in plants
BR0009510A (pt) * 1999-04-01 2002-04-23 Basf Plant Science Gmbh Enzima, sequência de nucleotìdeos, construção de gene, vetor, organismo ou célula transgênica, processo para a produção de triacilglicerol, triacilgliceróis, e, uso de uma sequência de nucleotìdeos
TWI377253B (en) 2001-04-16 2012-11-21 Martek Biosciences Corp Product and process for transformation of thraustochytriales microorganisms
US20040172682A1 (en) 2003-02-12 2004-09-02 Kinney Anthony J. Production of very long chain polyunsaturated fatty acids in oilseed plants
BRPI0407138A (pt) 2003-02-27 2006-01-10 Basf Plant Science Gmbh Sequência de ácido nucleico isolada, sequência de aminoácido, construção de gene, vetor, organismo transgênico não humano, processo para produzir ácidos graxos poliinsaturados, óleo, lipìdeo, ou um ácido graxo poliinsaturado ou uma fração dos mesmos, composições de óleo, de lipìdeos, ou de ácido graxo, e, uso do óleo, lipìdeos ou ácidos graxos ou de composições de óleo, de lipìdeos ou de ácido graxo
AU2004225838B2 (en) 2003-03-31 2009-11-05 University Of Bristol Novel plant acyltransferases specific for long-chained, multiply unsaturated fatty acids
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US7125672B2 (en) 2003-05-07 2006-10-24 E. I. Du Pont De Nemours And Company Codon-optimized genes for the production of polyunsaturated fatty acids in oleaginous yeasts
US8313911B2 (en) 2003-05-07 2012-11-20 E I Du Pont De Nemours And Company Production of polyunsaturated fatty acids in oleaginous yeasts
US7267976B2 (en) 2003-07-02 2007-09-11 E.I. Du Pont De Nemours And Company Acyltransferases for alteration of polyunsaturated fatty acids and oil content in oleaginous yeasts
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US7732155B2 (en) 2006-12-13 2010-06-08 National Research Council Of Canada Methods for identifying lysophosphatidylcholine acyltransferases
BRPI0808577A2 (pt) 2007-04-03 2019-09-24 E.I. Du Pont De Nemours And Company multienzima, polinuleotídeos isolados, construção recombinante, célula hospedeira, yarrowia sp. transformada, método de transformaçao celular, método para a produção de uma planta transformada, de leveduras, planta, sementes, óleos, produtos alimentícios ou rações, bebidas, moléculas isoladas de ácido nucléico, método de produção de uma multienzima, método para alterar o perfil de ácidos graxos, progênie, células hospedeiras microbianas recombinantes, métodos para conversão de ácido linoléico para ácido dihomo gamma-linolênico, ácido a-linolênico para ácido eicosatrienóico, ácido eicosapentaenóico para ácido docosahexaenóico, ácido araquidônico para ácido docosapentaenóico, método para a identificação de um polipeptídeo
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JP5346290B2 (ja) 2007-07-23 2013-11-20 サントリーホールディングス株式会社 新規な脂肪酸組成を有する脂肪酸組成物
US8323935B2 (en) 2007-10-03 2012-12-04 E I Du Pont De Nemours And Company Optimized strains of Yarrowia lipolytica for high eicosapentaenoic acid production
WO2009129582A1 (en) 2008-04-25 2009-10-29 Commonwealth Scientific Industrial Research Organisation Polypeptides and methods for producing triacylglycerols comprising modified fatty acids
WO2010033753A2 (en) * 2008-09-19 2010-03-25 E. I. Du Pont De Nemours And Company Mutant delta5 desaturases and their use in making polyunsaturated fatty acids
US8637298B2 (en) 2009-06-16 2014-01-28 E I Du Pont De Nemours And Company Optimized strains of yarrowia lipolytica for high eicosapentaenoic acid production
BRPI1009649A2 (pt) * 2009-06-16 2016-03-15 Du Pont célula hospedeira yarrowia sp, recombinante, método para fabricar um óleo microbiano e célula hospedeira recombinante para produção de um óleo
JP5931721B2 (ja) * 2009-06-16 2016-06-08 イー・アイ・デュポン・ドウ・ヌムール・アンド・カンパニーＥ．Ｉ．Ｄｕ Ｐｏｎｔ Ｄｅ Ｎｅｍｏｕｒｓ Ａｎｄ Ｃｏｍｐａｎｙ アシルＣｏＡリゾリン脂質アシルトランスフェラーゼの発現による長鎖オメガ−３及びオメガ−６多価不飽和脂肪酸生合成の改良
CA2807834C (en) 2010-08-26 2019-06-25 E. I. Du Pont De Nemours And Company Recombinant microbial host cells for high eicosapentaenoic acid production
WO2013192002A1 (en) * 2012-06-19 2013-12-27 E. I. Du Pont De Nemours And Company IMPROVED PRODUCTION OF POLYUNSATURATED FATTY ACIDS BY COEXPRESSION OF ACYL-CoA:LYSOPHOSPHATIDYLCHOLINE ACYLTRANSFERASES AND PHOSPHOLIPID:DIACYLGLYCEROL ACYLTRANSFERASES

Also Published As

Publication number Publication date
DK2861727T3 (en) 2018-06-14
EP2861727B1 (en) 2018-03-21
EP2861728B1 (en) 2017-12-27
JP6351582B2 (ja) 2018-07-04
CL2014003418A1 (es) 2015-08-28
JP2015519921A (ja) 2015-07-16
DK2861728T3 (en) 2018-03-26
BR112014031784A2 (pt) 2019-10-01
CA2877334A1 (en) 2013-12-27
WO2013192002A1 (en) 2013-12-27
EP2861727A1 (en) 2015-04-22
JP2015519920A (ja) 2015-07-16
BR112014031827A2 (pt) 2017-06-27
US9416382B2 (en) 2016-08-16
US20130337514A1 (en) 2013-12-19
EP2861728A1 (en) 2015-04-22
US9347075B2 (en) 2016-05-24
WO2013192007A1 (en) 2013-12-27
US20130337538A1 (en) 2013-12-19
CA2877279A1 (en) 2013-12-27

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