CL2016000299A1 - Metodo para la deteccion de resistencia a piretroides en crustaceos usando secuencias oligonucleotidas utiles en la deteccion de resistencia a piretroide. - Google Patents

Metodo para la deteccion de resistencia a piretroides en crustaceos usando secuencias oligonucleotidas utiles en la deteccion de resistencia a piretroide.

Info

Publication number
CL2016000299A1
CL2016000299A1 CL2016000299A CL2016000299A CL2016000299A1 CL 2016000299 A1 CL2016000299 A1 CL 2016000299A1 CL 2016000299 A CL2016000299 A CL 2016000299A CL 2016000299 A CL2016000299 A CL 2016000299A CL 2016000299 A1 CL2016000299 A1 CL 2016000299A1
Authority
CL
Chile
Prior art keywords
detection
pyrethroid resistance
crustaceans
pyrethroid
resistance
Prior art date
Application number
CL2016000299A
Other languages
English (en)
Inventor
Frank Nielsen
Per Gunnar Espedal
Original Assignee
Patogen Analyse Ass
Bergen Teknologioverfoering As
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Patogen Analyse Ass, Bergen Teknologioverfoering As filed Critical Patogen Analyse Ass
Publication of CL2016000299A1 publication Critical patent/CL2016000299A1/es

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • C12Q1/6888—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2600/00—Oligonucleotides characterized by their use
    • C12Q2600/156—Polymorphic or mutational markers

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Analytical Chemistry (AREA)
  • Zoology (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Microbiology (AREA)
  • Physics & Mathematics (AREA)
  • Molecular Biology (AREA)
  • Immunology (AREA)
  • Biotechnology (AREA)
  • Biophysics (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

Un método in vitro para la detección de resistencia a los piretroides en uno o más crustáceos, CARACTERIZADO porque comprende las etapas de a) analizar el material genético mitocondrial del crustáceo, b) detección de uno o más polimorfismos de nucleótido único (SNP) asociado con la resistencia a piretroides en el ADN mitocondrial del crustáceo a ser analizado. Se refiere a las secuencias que comprenden un polimorfismo de nucleótido pequeño (SNP) asociados con resistencia a piretroides en crustáceos, y en el que los SNP están presentes en el ADN mitocondrial del crustáceo y donde dicho oligonucleótido aislado comprende al menos 8 nucleótidos continuos de la secuencia seleccionada del grupo que consiste de la SEQ ID No 4 y SEQ ID No 5, y al uso de dichas secuencias para la ejecución del método antes descrito.
CL2016000299A 2013-08-06 2016-02-05 Metodo para la deteccion de resistencia a piretroides en crustaceos usando secuencias oligonucleotidas utiles en la deteccion de resistencia a piretroide. CL2016000299A1 (es)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
NO20131075 2013-08-06

Publications (1)

Publication Number Publication Date
CL2016000299A1 true CL2016000299A1 (es) 2016-11-04

Family

ID=51301266

Family Applications (1)

Application Number Title Priority Date Filing Date
CL2016000299A CL2016000299A1 (es) 2013-08-06 2016-02-05 Metodo para la deteccion de resistencia a piretroides en crustaceos usando secuencias oligonucleotidas utiles en la deteccion de resistencia a piretroide.

Country Status (5)

Country Link
EP (1) EP3030674B1 (es)
CA (1) CA2920588A1 (es)
CL (1) CL2016000299A1 (es)
DK (1) DK179775B1 (es)
WO (1) WO2015018861A1 (es)

Families Citing this family (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP4085155A1 (en) 2020-01-02 2022-11-09 Patogen As Method for detecting hydrogen peroxide resistance in crustaceans
CN112301098A (zh) * 2020-10-22 2021-02-02 深圳市卫生健康发展研究中心 人线粒体dna全序列扩增的方法以及用于人线粒体dna全序列扩增的试剂盒

Family Cites Families (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6027876A (en) * 1992-12-30 2000-02-22 Kreitman; Martin Method for monitoring pesticide resistance
WO2001014592A2 (en) * 1999-08-25 2001-03-01 Clarity Biosciences, Inc. Identifying organisms by detecting intronic nucleic acid or encoded proteins
US8178503B2 (en) * 2006-03-03 2012-05-15 International Business Machines Corporation Ribonucleic acid interference molecules and binding sites derived by analyzing intergenic and intronic regions of genomes
US7901882B2 (en) * 2006-03-31 2011-03-08 Affymetrix, Inc. Analysis of methylation using nucleic acid arrays

Also Published As

Publication number Publication date
EP3030674A1 (en) 2016-06-15
WO2015018861A1 (en) 2015-02-12
DK201600137A1 (en) 2016-04-04
CA2920588A1 (en) 2015-02-12
EP3030674B1 (en) 2021-04-14
DK179775B1 (en) 2019-05-22

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