CL2016003427A1 - Una secuencia peptídica de una proteína guía para la producción de un péptido de interés; un vector de expresión; una célula huésped; un proceso para para la producción de un péptido de interés. - Google Patents

Una secuencia peptídica de una proteína guía para la producción de un péptido de interés; un vector de expresión; una célula huésped; un proceso para para la producción de un péptido de interés.

Info

Publication number
CL2016003427A1
CL2016003427A1 CL2016003427A CL2016003427A CL2016003427A1 CL 2016003427 A1 CL2016003427 A1 CL 2016003427A1 CL 2016003427 A CL2016003427 A CL 2016003427A CL 2016003427 A CL2016003427 A CL 2016003427A CL 2016003427 A1 CL2016003427 A1 CL 2016003427A1
Authority
CL
Chile
Prior art keywords
peptide
interest
production
host cell
expression vector
Prior art date
Application number
CL2016003427A
Other languages
English (en)
Inventor
Anne Andrews Farrow Bárbara
Alfonso Asenjo De Leuze Juan
Rodríguez Gallardo Vida
Original Assignee
Univ Chile
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Univ Chile filed Critical Univ Chile
Priority to CL2016003427A priority Critical patent/CL2016003427A1/es
Publication of CL2016003427A1 publication Critical patent/CL2016003427A1/es
Priority to US16/474,805 priority patent/US11261472B2/en
Priority to PCT/IB2017/058480 priority patent/WO2018122777A1/es

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P21/00—Preparation of peptides or proteins
    • C12P21/02—Preparation of peptides or proteins having a known sequence of two or more amino acids, e.g. glutathione
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/70—Vectors or expression systems specially adapted for E. coli
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/46—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/46—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates
    • C07K14/47—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates from mammals
    • C07K14/4701—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates from mammals not used
    • C07K14/4746—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates from mammals not used p53
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K19/00—Hybrid peptides, i.e. peptides covalently bound to nucleic acids, or non-covalently bound protein-protein complexes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/62—DNA sequences coding for fusion proteins
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/67—General methods for enhancing the expression
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y304/00—Hydrolases acting on peptide bonds, i.e. peptidases (3.4)
    • C12Y304/21—Serine endopeptidases (3.4.21)
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/01—Fusion polypeptide containing a localisation/targetting motif
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/01—Fusion polypeptide containing a localisation/targetting motif
    • C07K2319/10—Fusion polypeptide containing a localisation/targetting motif containing a tag for extracellular membrane crossing, e.g. TAT or VP22
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/50—Fusion polypeptide containing protease site

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Biochemistry (AREA)
  • Molecular Biology (AREA)
  • General Health & Medical Sciences (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • Biophysics (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Microbiology (AREA)
  • Medicinal Chemistry (AREA)
  • Plant Pathology (AREA)
  • Physics & Mathematics (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Toxicology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Peptides Or Proteins (AREA)
CL2016003427A 2016-12-30 2016-12-30 Una secuencia peptídica de una proteína guía para la producción de un péptido de interés; un vector de expresión; una célula huésped; un proceso para para la producción de un péptido de interés. CL2016003427A1 (es)

Priority Applications (3)

Application Number Priority Date Filing Date Title
CL2016003427A CL2016003427A1 (es) 2016-12-30 2016-12-30 Una secuencia peptídica de una proteína guía para la producción de un péptido de interés; un vector de expresión; una célula huésped; un proceso para para la producción de un péptido de interés.
US16/474,805 US11261472B2 (en) 2016-12-30 2017-12-28 Peptide sequence of a guide protein for the production of a peptide of interest, an expression vector, a host cell, and a method for the production of a peptide of interest
PCT/IB2017/058480 WO2018122777A1 (es) 2016-12-30 2017-12-28 Una secuencia peptídica de una proteína guía para la producción de un péptido de interés; un vector de expresión; una célula huésped; un proceso para para la producción de un péptido de interés

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
CL2016003427A CL2016003427A1 (es) 2016-12-30 2016-12-30 Una secuencia peptídica de una proteína guía para la producción de un péptido de interés; un vector de expresión; una célula huésped; un proceso para para la producción de un péptido de interés.

Publications (1)

Publication Number Publication Date
CL2016003427A1 true CL2016003427A1 (es) 2017-06-23

Family

ID=59858446

Family Applications (1)

Application Number Title Priority Date Filing Date
CL2016003427A CL2016003427A1 (es) 2016-12-30 2016-12-30 Una secuencia peptídica de una proteína guía para la producción de un péptido de interés; un vector de expresión; una célula huésped; un proceso para para la producción de un péptido de interés.

Country Status (3)

Country Link
US (1) US11261472B2 (es)
CL (1) CL2016003427A1 (es)
WO (1) WO2018122777A1 (es)

Families Citing this family (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN117164722B (zh) * 2023-08-09 2024-04-09 东北农业大学 一种螺旋束damp4-pr-fo融合蛋白d2l及其制备方法和应用
CN117965667B (zh) * 2024-01-23 2024-08-16 陕西普罗安蒂生物科技发展有限公司 一种通过切割融合蛋白制备多肽的方法

Family Cites Families (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0796335A1 (en) 1994-12-07 1997-09-24 Bionebraska, Inc. Production of peptides using recombinant fusion protein constructs
US7072771B2 (en) * 2001-06-07 2006-07-04 University Of Kentucky Research Foundation Selective PARP-1 targeting for designing chemo/radio sensitizing agents
US20100234568A1 (en) 2006-10-19 2010-09-16 Linda Jane Decarolis Identification of peptide tags for the production of insoluble peptides by sequence scanning
US8796431B2 (en) 2009-11-09 2014-08-05 The Regents Of The University Of Colorado, A Body Corporate Efficient production of peptides
US9714274B2 (en) 2012-03-23 2017-07-25 Suzhou Kunpeng Biotech Co., Ltd. Fusion proteins of superfolder green fluorescent protein and use thereof

Also Published As

Publication number Publication date
US20200385774A1 (en) 2020-12-10
WO2018122777A1 (es) 2018-07-05
US11261472B2 (en) 2022-03-01

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